Amino acid dipepetide frequency for Bacillus phage vB_BthS_BMBphi

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
1.671AlaAla: 1.671 ± 0.728
0.348AlaCys: 0.348 ± 0.167
3.411AlaAsp: 3.411 ± 0.467
4.386AlaGlu: 4.386 ± 0.692
3.133AlaPhe: 3.133 ± 0.498
3.899AlaGly: 3.899 ± 1.015
1.044AlaHis: 1.044 ± 0.252
3.411AlaIle: 3.411 ± 0.464
5.291AlaLys: 5.291 ± 0.662
5.221AlaLeu: 5.221 ± 0.571
1.392AlaMet: 1.392 ± 0.331
2.228AlaAsn: 2.228 ± 0.347
2.576AlaPro: 2.576 ± 0.42
2.646AlaGln: 2.646 ± 0.505
2.854AlaArg: 2.854 ± 0.477
4.456AlaSer: 4.456 ± 0.608
4.247AlaThr: 4.247 ± 0.667
3.272AlaVal: 3.272 ± 0.438
0.766AlaTrp: 0.766 ± 0.296
2.715AlaTyr: 2.715 ± 0.589
0.0AlaXaa: 0.0 ± 0.0
Cys
0.278CysAla: 0.278 ± 0.136
0.139CysCys: 0.139 ± 0.109
0.975CysAsp: 0.975 ± 0.255
0.418CysGlu: 0.418 ± 0.128
0.348CysPhe: 0.348 ± 0.192
0.627CysGly: 0.627 ± 0.218
0.348CysHis: 0.348 ± 0.149
0.557CysIle: 0.557 ± 0.234
0.696CysLys: 0.696 ± 0.187
0.627CysLeu: 0.627 ± 0.178
0.209CysMet: 0.209 ± 0.123
0.418CysAsn: 0.418 ± 0.215
0.418CysPro: 0.418 ± 0.165
0.627CysGln: 0.627 ± 0.231
0.487CysArg: 0.487 ± 0.169
0.418CysSer: 0.418 ± 0.161
0.835CysThr: 0.835 ± 0.216
0.278CysVal: 0.278 ± 0.137
0.07CysTrp: 0.07 ± 0.07
0.348CysTyr: 0.348 ± 0.171
0.0CysXaa: 0.0 ± 0.0
Asp
3.411AspAla: 3.411 ± 0.429
0.696AspCys: 0.696 ± 0.205
2.367AspAsp: 2.367 ± 0.408
4.386AspGlu: 4.386 ± 0.547
2.994AspPhe: 2.994 ± 0.581
4.804AspGly: 4.804 ± 0.553
0.418AspHis: 0.418 ± 0.17
5.709AspIle: 5.709 ± 0.642
6.335AspLys: 6.335 ± 0.747
4.247AspLeu: 4.247 ± 0.369
1.671AspMet: 1.671 ± 0.345
2.297AspAsn: 2.297 ± 0.406
1.601AspPro: 1.601 ± 0.321
1.114AspGln: 1.114 ± 0.296
2.576AspArg: 2.576 ± 0.452
3.342AspSer: 3.342 ± 0.497
3.551AspThr: 3.551 ± 0.41
4.107AspVal: 4.107 ± 0.416
0.557AspTrp: 0.557 ± 0.199
2.576AspTyr: 2.576 ± 0.387
0.0AspXaa: 0.0 ± 0.0
Glu
4.873GluAla: 4.873 ± 0.559
0.905GluCys: 0.905 ± 0.317
5.082GluAsp: 5.082 ± 0.611
7.449GluGlu: 7.449 ± 1.057
3.411GluPhe: 3.411 ± 0.418
5.152GluGly: 5.152 ± 0.47
1.601GluHis: 1.601 ± 0.345
5.778GluIle: 5.778 ± 0.563
5.221GluLys: 5.221 ± 0.577
6.057GluLeu: 6.057 ± 0.647
3.202GluMet: 3.202 ± 0.47
4.456GluAsn: 4.456 ± 0.617
1.601GluPro: 1.601 ± 0.337
2.576GluGln: 2.576 ± 0.513
4.177GluArg: 4.177 ± 0.599
4.038GluSer: 4.038 ± 0.617
4.177GluThr: 4.177 ± 0.51
6.614GluVal: 6.614 ± 0.791
1.88GluTrp: 1.88 ± 0.301
4.247GluTyr: 4.247 ± 0.593
0.0GluXaa: 0.0 ± 0.0
Phe
2.437PheAla: 2.437 ± 0.458
0.487PheCys: 0.487 ± 0.169
2.158PheAsp: 2.158 ± 0.469
3.411PheGlu: 3.411 ± 0.506
1.044PhePhe: 1.044 ± 0.26
2.854PheGly: 2.854 ± 0.473
0.835PheHis: 0.835 ± 0.281
2.785PheIle: 2.785 ± 0.407
3.69PheLys: 3.69 ± 0.464
2.785PheLeu: 2.785 ± 0.38
1.601PheMet: 1.601 ± 0.34
2.506PheAsn: 2.506 ± 0.368
1.392PhePro: 1.392 ± 0.34
1.323PheGln: 1.323 ± 0.348
2.297PheArg: 2.297 ± 0.362
2.506PheSer: 2.506 ± 0.425
2.854PheThr: 2.854 ± 0.451
1.74PheVal: 1.74 ± 0.346
0.418PheTrp: 0.418 ± 0.174
1.949PheTyr: 1.949 ± 0.343
0.0PheXaa: 0.0 ± 0.0
Gly
3.272GlyAla: 3.272 ± 0.721
0.696GlyCys: 0.696 ± 0.219
4.107GlyAsp: 4.107 ± 0.534
5.291GlyGlu: 5.291 ± 0.655
3.202GlyPhe: 3.202 ± 0.44
4.804GlyGly: 4.804 ± 0.834
1.044GlyHis: 1.044 ± 0.268
5.152GlyIle: 5.152 ± 0.712
5.848GlyLys: 5.848 ± 0.558
4.804GlyLeu: 4.804 ± 0.601
1.81GlyMet: 1.81 ± 0.309
3.759GlyAsn: 3.759 ± 0.636
0.0GlyPro: 0.0 ± 0.0
2.228GlyGln: 2.228 ± 0.42
3.272GlyArg: 3.272 ± 0.499
2.924GlySer: 2.924 ± 0.619
4.316GlyThr: 4.316 ± 0.557
3.899GlyVal: 3.899 ± 0.632
1.184GlyTrp: 1.184 ± 0.259
3.481GlyTyr: 3.481 ± 0.674
0.0GlyXaa: 0.0 ± 0.0
His
1.323HisAla: 1.323 ± 0.282
0.139HisCys: 0.139 ± 0.125
0.627HisAsp: 0.627 ± 0.217
1.253HisGlu: 1.253 ± 0.351
0.835HisPhe: 0.835 ± 0.226
1.253HisGly: 1.253 ± 0.29
0.209HisHis: 0.209 ± 0.127
1.74HisIle: 1.74 ± 0.424
1.392HisLys: 1.392 ± 0.35
1.532HisLeu: 1.532 ± 0.343
0.766HisMet: 0.766 ± 0.274
1.044HisAsn: 1.044 ± 0.235
0.975HisPro: 0.975 ± 0.243
0.348HisGln: 0.348 ± 0.156
1.532HisArg: 1.532 ± 0.371
1.184HisSer: 1.184 ± 0.221
0.348HisThr: 0.348 ± 0.153
1.114HisVal: 1.114 ± 0.293
0.348HisTrp: 0.348 ± 0.137
0.766HisTyr: 0.766 ± 0.256
0.0HisXaa: 0.0 ± 0.0
Ile
3.69IleAla: 3.69 ± 0.488
0.627IleCys: 0.627 ± 0.179
4.943IleAsp: 4.943 ± 0.494
5.987IleGlu: 5.987 ± 0.677
2.089IlePhe: 2.089 ± 0.319
3.202IleGly: 3.202 ± 0.432
1.253IleHis: 1.253 ± 0.305
3.899IleIle: 3.899 ± 0.559
6.405IleLys: 6.405 ± 0.739
4.664IleLeu: 4.664 ± 0.608
1.462IleMet: 1.462 ± 0.324
3.829IleAsn: 3.829 ± 0.568
2.715IlePro: 2.715 ± 0.474
1.532IleGln: 1.532 ± 0.334
3.063IleArg: 3.063 ± 0.447
4.247IleSer: 4.247 ± 0.553
3.759IleThr: 3.759 ± 0.579
4.664IleVal: 4.664 ± 0.596
0.766IleTrp: 0.766 ± 0.213
2.715IleTyr: 2.715 ± 0.492
0.0IleXaa: 0.0 ± 0.0
Lys
5.569LysAla: 5.569 ± 0.806
0.627LysCys: 0.627 ± 0.224
5.082LysAsp: 5.082 ± 0.597
8.215LysGlu: 8.215 ± 0.979
3.481LysPhe: 3.481 ± 0.528
5.291LysGly: 5.291 ± 0.633
2.646LysHis: 2.646 ± 0.422
4.664LysIle: 4.664 ± 0.516
8.006LysLys: 8.006 ± 0.944
6.475LysLeu: 6.475 ± 0.559
2.297LysMet: 2.297 ± 0.434
4.038LysAsn: 4.038 ± 0.511
3.69LysPro: 3.69 ± 0.554
3.272LysGln: 3.272 ± 0.479
3.481LysArg: 3.481 ± 0.497
3.968LysSer: 3.968 ± 0.49
3.759LysThr: 3.759 ± 0.461
5.152LysVal: 5.152 ± 0.537
1.532LysTrp: 1.532 ± 0.254
3.411LysTyr: 3.411 ± 0.49
0.0LysXaa: 0.0 ± 0.0
Leu
5.709LeuAla: 5.709 ± 0.681
0.278LeuCys: 0.278 ± 0.152
4.664LeuAsp: 4.664 ± 0.587
7.38LeuGlu: 7.38 ± 0.754
2.437LeuPhe: 2.437 ± 0.398
5.569LeuGly: 5.569 ± 0.871
1.392LeuHis: 1.392 ± 0.287
5.013LeuIle: 5.013 ± 0.555
5.848LeuLys: 5.848 ± 0.664
4.734LeuLeu: 4.734 ± 0.612
1.949LeuMet: 1.949 ± 0.386
4.316LeuAsn: 4.316 ± 0.618
3.411LeuPro: 3.411 ± 0.589
3.551LeuGln: 3.551 ± 0.505
3.551LeuArg: 3.551 ± 0.471
4.107LeuSer: 4.107 ± 0.401
4.943LeuThr: 4.943 ± 0.484
5.082LeuVal: 5.082 ± 0.561
0.975LeuTrp: 0.975 ± 0.251
2.576LeuTyr: 2.576 ± 0.425
0.0LeuXaa: 0.0 ± 0.0
Met
2.297MetAla: 2.297 ± 0.494
0.139MetCys: 0.139 ± 0.088
1.323MetAsp: 1.323 ± 0.342
2.646MetGlu: 2.646 ± 0.447
0.835MetPhe: 0.835 ± 0.209
1.532MetGly: 1.532 ± 0.272
0.348MetHis: 0.348 ± 0.152
1.949MetIle: 1.949 ± 0.372
3.202MetLys: 3.202 ± 0.384
1.392MetLeu: 1.392 ± 0.319
0.557MetMet: 0.557 ± 0.184
1.949MetAsn: 1.949 ± 0.437
0.418MetPro: 0.418 ± 0.163
0.835MetGln: 0.835 ± 0.268
1.949MetArg: 1.949 ± 0.309
2.437MetSer: 2.437 ± 0.476
2.158MetThr: 2.158 ± 0.367
1.253MetVal: 1.253 ± 0.31
0.278MetTrp: 0.278 ± 0.124
1.114MetTyr: 1.114 ± 0.277
0.0MetXaa: 0.0 ± 0.0
Asn
3.411AsnAla: 3.411 ± 0.424
0.557AsnCys: 0.557 ± 0.198
3.063AsnAsp: 3.063 ± 0.38
3.481AsnGlu: 3.481 ± 0.482
2.297AsnPhe: 2.297 ± 0.405
3.968AsnGly: 3.968 ± 0.498
1.323AsnHis: 1.323 ± 0.438
3.62AsnIle: 3.62 ± 0.493
3.342AsnLys: 3.342 ± 0.538
6.057AsnLeu: 6.057 ± 0.929
1.949AsnMet: 1.949 ± 0.374
3.063AsnAsn: 3.063 ± 0.505
2.924AsnPro: 2.924 ± 0.532
1.253AsnGln: 1.253 ± 0.279
2.924AsnArg: 2.924 ± 0.481
2.646AsnSer: 2.646 ± 0.408
3.133AsnThr: 3.133 ± 0.592
2.228AsnVal: 2.228 ± 0.411
0.696AsnTrp: 0.696 ± 0.245
2.437AsnTyr: 2.437 ± 0.491
0.0AsnXaa: 0.0 ± 0.0
Pro
1.323ProAla: 1.323 ± 0.318
0.209ProCys: 0.209 ± 0.105
1.81ProAsp: 1.81 ± 0.361
2.854ProGlu: 2.854 ± 0.517
1.81ProPhe: 1.81 ± 0.368
0.07ProGly: 0.07 ± 0.067
0.696ProHis: 0.696 ± 0.277
2.228ProIle: 2.228 ± 0.333
2.506ProLys: 2.506 ± 0.533
2.715ProLeu: 2.715 ± 0.436
0.557ProMet: 0.557 ± 0.235
2.019ProAsn: 2.019 ± 0.431
1.323ProPro: 1.323 ± 0.373
1.74ProGln: 1.74 ± 0.357
1.323ProArg: 1.323 ± 0.309
2.854ProSer: 2.854 ± 0.497
2.228ProThr: 2.228 ± 0.4
2.228ProVal: 2.228 ± 0.47
0.418ProTrp: 0.418 ± 0.182
1.323ProTyr: 1.323 ± 0.352
0.0ProXaa: 0.0 ± 0.0
Gln
2.715GlnAla: 2.715 ± 0.457
0.418GlnCys: 0.418 ± 0.176
1.671GlnAsp: 1.671 ± 0.354
3.063GlnGlu: 3.063 ± 0.48
1.323GlnPhe: 1.323 ± 0.262
2.576GlnGly: 2.576 ± 0.45
0.696GlnHis: 0.696 ± 0.195
1.949GlnIle: 1.949 ± 0.38
2.785GlnLys: 2.785 ± 0.478
3.829GlnLeu: 3.829 ± 0.492
1.114GlnMet: 1.114 ± 0.349
1.532GlnAsn: 1.532 ± 0.312
0.905GlnPro: 0.905 ± 0.271
1.81GlnGln: 1.81 ± 0.337
1.949GlnArg: 1.949 ± 0.347
2.228GlnSer: 2.228 ± 0.479
1.671GlnThr: 1.671 ± 0.401
2.646GlnVal: 2.646 ± 0.461
0.278GlnTrp: 0.278 ± 0.141
1.392GlnTyr: 1.392 ± 0.326
0.0GlnXaa: 0.0 ± 0.0
Arg
2.089ArgAla: 2.089 ± 0.337
0.487ArgCys: 0.487 ± 0.203
2.576ArgAsp: 2.576 ± 0.414
4.525ArgGlu: 4.525 ± 0.713
2.367ArgPhe: 2.367 ± 0.346
3.342ArgGly: 3.342 ± 0.415
0.696ArgHis: 0.696 ± 0.198
2.924ArgIle: 2.924 ± 0.386
4.386ArgLys: 4.386 ± 0.615
3.759ArgLeu: 3.759 ± 0.533
1.392ArgMet: 1.392 ± 0.333
3.133ArgAsn: 3.133 ± 0.405
0.835ArgPro: 0.835 ± 0.279
2.297ArgGln: 2.297 ± 0.354
2.367ArgArg: 2.367 ± 0.396
2.437ArgSer: 2.437 ± 0.355
2.576ArgThr: 2.576 ± 0.439
3.551ArgVal: 3.551 ± 0.477
0.835ArgTrp: 0.835 ± 0.237
2.019ArgTyr: 2.019 ± 0.294
0.0ArgXaa: 0.0 ± 0.0
Ser
3.69SerAla: 3.69 ± 0.512
0.348SerCys: 0.348 ± 0.146
3.62SerAsp: 3.62 ± 0.464
4.456SerGlu: 4.456 ± 0.506
1.949SerPhe: 1.949 ± 0.385
4.386SerGly: 4.386 ± 0.623
0.975SerHis: 0.975 ± 0.277
3.968SerIle: 3.968 ± 0.458
4.873SerLys: 4.873 ± 0.484
4.664SerLeu: 4.664 ± 0.646
1.671SerMet: 1.671 ± 0.309
3.968SerAsn: 3.968 ± 0.464
2.228SerPro: 2.228 ± 0.377
2.785SerGln: 2.785 ± 0.429
2.854SerArg: 2.854 ± 0.431
3.202SerSer: 3.202 ± 0.516
3.551SerThr: 3.551 ± 0.466
2.715SerVal: 2.715 ± 0.348
0.627SerTrp: 0.627 ± 0.188
2.576SerTyr: 2.576 ± 0.462
0.0SerXaa: 0.0 ± 0.0
Thr
3.69ThrAla: 3.69 ± 0.63
0.696ThrCys: 0.696 ± 0.232
3.759ThrAsp: 3.759 ± 0.385
3.481ThrGlu: 3.481 ± 0.437
3.411ThrPhe: 3.411 ± 0.607
4.664ThrGly: 4.664 ± 0.669
0.905ThrHis: 0.905 ± 0.303
3.342ThrIle: 3.342 ± 0.55
4.107ThrLys: 4.107 ± 0.512
4.107ThrLeu: 4.107 ± 0.501
1.323ThrMet: 1.323 ± 0.333
2.646ThrAsn: 2.646 ± 0.442
1.88ThrPro: 1.88 ± 0.309
2.854ThrGln: 2.854 ± 0.44
2.019ThrArg: 2.019 ± 0.323
4.316ThrSer: 4.316 ± 0.687
3.133ThrThr: 3.133 ± 0.415
4.316ThrVal: 4.316 ± 0.457
1.462ThrTrp: 1.462 ± 0.292
2.715ThrTyr: 2.715 ± 0.512
0.0ThrXaa: 0.0 ± 0.0
Val
4.595ValAla: 4.595 ± 0.591
0.557ValCys: 0.557 ± 0.177
3.899ValAsp: 3.899 ± 0.577
4.943ValGlu: 4.943 ± 0.674
2.089ValPhe: 2.089 ± 0.376
3.551ValGly: 3.551 ± 0.427
1.253ValHis: 1.253 ± 0.277
4.038ValIle: 4.038 ± 0.495
5.918ValLys: 5.918 ± 0.696
4.595ValLeu: 4.595 ± 0.479
1.323ValMet: 1.323 ± 0.344
3.411ValAsn: 3.411 ± 0.51
1.88ValPro: 1.88 ± 0.357
1.74ValGln: 1.74 ± 0.472
2.297ValArg: 2.297 ± 0.439
4.316ValSer: 4.316 ± 0.526
3.968ValThr: 3.968 ± 0.631
3.968ValVal: 3.968 ± 0.533
0.975ValTrp: 0.975 ± 0.216
2.506ValTyr: 2.506 ± 0.372
0.0ValXaa: 0.0 ± 0.0
Trp
0.835TrpAla: 0.835 ± 0.184
0.418TrpCys: 0.418 ± 0.168
0.557TrpAsp: 0.557 ± 0.198
1.671TrpGlu: 1.671 ± 0.44
0.348TrpPhe: 0.348 ± 0.19
0.766TrpGly: 0.766 ± 0.222
0.418TrpHis: 0.418 ± 0.169
0.696TrpIle: 0.696 ± 0.209
1.949TrpLys: 1.949 ± 0.426
1.184TrpLeu: 1.184 ± 0.278
0.696TrpMet: 0.696 ± 0.216
1.323TrpAsn: 1.323 ± 0.283
0.0TrpPro: 0.0 ± 0.0
0.696TrpGln: 0.696 ± 0.324
0.696TrpArg: 0.696 ± 0.194
0.696TrpSer: 0.696 ± 0.216
0.835TrpThr: 0.835 ± 0.251
0.557TrpVal: 0.557 ± 0.217
0.07TrpTrp: 0.07 ± 0.076
0.209TrpTyr: 0.209 ± 0.117
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.089TyrAla: 2.089 ± 0.407
0.348TyrCys: 0.348 ± 0.137
3.063TyrAsp: 3.063 ± 0.41
3.202TyrGlu: 3.202 ± 0.431
1.671TyrPhe: 1.671 ± 0.384
2.785TyrGly: 2.785 ± 0.405
0.627TyrHis: 0.627 ± 0.209
2.089TyrIle: 2.089 ± 0.341
2.924TyrLys: 2.924 ± 0.536
4.038TyrLeu: 4.038 ± 0.594
1.601TyrMet: 1.601 ± 0.337
2.437TyrAsn: 2.437 ± 0.37
1.462TyrPro: 1.462 ± 0.353
1.323TyrGln: 1.323 ± 0.333
2.924TyrArg: 2.924 ± 0.528
2.785TyrSer: 2.785 ± 0.4
2.785TyrThr: 2.785 ± 0.499
2.437TyrVal: 2.437 ± 0.404
0.487TyrTrp: 0.487 ± 0.179
2.297TyrTyr: 2.297 ± 0.389
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 79 proteins (14365 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski