Rhodotorula diobovata

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Basidiomycota; Pucciniomycotina; Microbotryomycetes; Sporidiobolales; Sporidiobolaceae; Rhodotorula

Average proteome isoelectric point is 6.82

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7905 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5C5FM42|A0A5C5FM42_9BASI C2 domain-containing protein OS=Rhodotorula diobovata OX=5288 GN=DMC30DRAFT_126338 PE=4 SV=1
MM1 pKa = 7.56HH2 pKa = 7.29ARR4 pKa = 11.84SLLSLAVLATSAVALSPADD23 pKa = 3.82FSRR26 pKa = 11.84LARR29 pKa = 11.84RR30 pKa = 11.84DD31 pKa = 3.56LASDD35 pKa = 3.8TAAAMDD41 pKa = 3.97AATALGQAAVTAAGDD56 pKa = 3.78NQCQDD61 pKa = 3.76TCPQWLDD68 pKa = 3.33SATACATDD76 pKa = 3.48VTDD79 pKa = 3.53VAGTLEE85 pKa = 4.59CACSEE90 pKa = 4.44DD91 pKa = 4.28FLSSMKK97 pKa = 10.59ACAACIAEE105 pKa = 4.75DD106 pKa = 4.61APSQAQTISDD116 pKa = 3.93TCASAAASMSDD127 pKa = 3.45GSSSLPQSAASSASSAISSGASSVSSAFSSASSAASSAASSASSAASSAGSASASGTAPAASASGEE193 pKa = 4.33SGASTGKK200 pKa = 8.33TLFAGAAAAVGAAAVAMLAA219 pKa = 3.93

Molecular weight: 20.42 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5C5FMR0|A0A5C5FMR0_9BASI Uncharacterized protein OS=Rhodotorula diobovata OX=5288 GN=DMC30DRAFT_449631 PE=3 SV=1
GG1 pKa = 6.72TPPSSSSLTLSLAPSLTPHH20 pKa = 5.73SHH22 pKa = 6.55LALVAKK28 pKa = 9.77VRR30 pKa = 11.84NAVQSSLRR38 pKa = 11.84LQSSQAVRR46 pKa = 11.84LKK48 pKa = 9.98PSPRR52 pKa = 11.84RR53 pKa = 11.84RR54 pKa = 11.84RR55 pKa = 11.84RR56 pKa = 11.84RR57 pKa = 11.84RR58 pKa = 11.84RR59 pKa = 11.84RR60 pKa = 11.84RR61 pKa = 11.84VRR63 pKa = 11.84VPHH66 pKa = 6.3LRR68 pKa = 11.84LLFLLLRR75 pKa = 11.84LHH77 pKa = 6.34QSSLPPRR84 pKa = 11.84SRR86 pKa = 11.84SLFALAKK93 pKa = 7.87GTAWLALRR101 pKa = 11.84SSTRR105 pKa = 11.84APRR108 pKa = 11.84RR109 pKa = 11.84TCSTLASSGVSTPPSTLTLRR129 pKa = 11.84LTVAAFASPFLSTFRR144 pKa = 11.84SKK146 pKa = 10.77LARR149 pKa = 11.84VPLLPVRR156 pKa = 11.84SSSLHH161 pKa = 6.03FPTASTPFSASCGSVIPARR180 pKa = 11.84GSLPITSSTFPQASRR195 pKa = 11.84RR196 pKa = 11.84RR197 pKa = 11.84ASTTSRR203 pKa = 11.84RR204 pKa = 11.84RR205 pKa = 11.84PLPLSRR211 pKa = 11.84IATLSTSAFLAAPP224 pKa = 4.42

Molecular weight: 24.29 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7905

0

7905

4044272

49

5391

511.6

55.08

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.189 ± 0.04

1.086 ± 0.011

5.601 ± 0.023

5.836 ± 0.023

3.135 ± 0.016

7.839 ± 0.024

2.351 ± 0.013

2.908 ± 0.021

3.665 ± 0.025

9.399 ± 0.031

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.503 ± 0.009

2.14 ± 0.014

7.481 ± 0.039

3.446 ± 0.02

7.402 ± 0.03

8.802 ± 0.036

5.467 ± 0.016

6.427 ± 0.021

1.274 ± 0.009

2.047 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski