uncultured SUP05 cluster bacterium

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Gammaproteobacteria incertae sedis; Candidatus Thioglobus; environmental samples

Average proteome isoelectric point is 6.4

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1283 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D1KBD7|D1KBD7_9GAMM Protein-tyrosine-phosphatase OS=uncultured SUP05 cluster bacterium OX=655186 GN=Sup05_0233 PE=4 SV=1
MM1 pKa = 6.86VTTIDD6 pKa = 4.28ADD8 pKa = 4.0LQAMPSTTWSAWVKK22 pKa = 10.73PNGTSGWQVIFGMEE36 pKa = 3.84DD37 pKa = 3.34GGWDD41 pKa = 3.0RR42 pKa = 11.84FLILEE47 pKa = 4.4SGSLNVSMGHH57 pKa = 6.01TSNRR61 pKa = 11.84WQTGATLTNDD71 pKa = 3.07VWQHH75 pKa = 4.35VASIYY80 pKa = 11.12DD81 pKa = 3.4NGTMKK86 pKa = 10.56FYY88 pKa = 11.25LNGTEE93 pKa = 4.24YY94 pKa = 8.16TTGTDD99 pKa = 3.48EE100 pKa = 4.97GNHH103 pKa = 5.93SSTGKK108 pKa = 8.63FTIGGNQNGASNLYY122 pKa = 9.06VGRR125 pKa = 11.84VDD127 pKa = 5.83EE128 pKa = 4.26VAVWNEE134 pKa = 3.45ALTKK138 pKa = 10.76DD139 pKa = 4.51EE140 pKa = 5.31IVALYY145 pKa = 10.86NSGSPLLASSDD156 pKa = 3.68SGDD159 pKa = 3.65YY160 pKa = 10.78ASSGNLVGYY169 pKa = 7.94YY170 pKa = 10.06TMNSNDD176 pKa = 3.28GSGTSVTDD184 pKa = 3.58DD185 pKa = 3.58SGNSEE190 pKa = 4.18TATITGGSIWSTDD203 pKa = 3.02VVAITDD209 pKa = 3.69TTAPTLSSSTPADD222 pKa = 3.24NAASVAANANIVLNFSEE239 pKa = 4.5AVDD242 pKa = 3.87VEE244 pKa = 4.58SGKK247 pKa = 8.53ITIKK251 pKa = 9.69KK252 pKa = 8.47TSDD255 pKa = 2.89NSTVATIAVGDD266 pKa = 3.97AQVTGTGTTAITINPTNDD284 pKa = 2.9LSGGTEE290 pKa = 4.31YY291 pKa = 11.25YY292 pKa = 11.16VLIDD296 pKa = 3.42ATAFDD301 pKa = 4.95DD302 pKa = 4.58SSSNSYY308 pKa = 11.08AGISSTTALSFTTADD323 pKa = 3.52TTSPTISSTTVAADD337 pKa = 3.3NPTIDD342 pKa = 3.29VTFNEE347 pKa = 4.49AVFNATGGSGALQASDD363 pKa = 3.42FTISISGGTKK373 pKa = 8.81TLSSATPSSISISGNVYY390 pKa = 8.9TLGLDD395 pKa = 4.28LSGTANGSEE404 pKa = 4.61TITVVPSSSTAIYY417 pKa = 10.4DD418 pKa = 3.73GSDD421 pKa = 3.11NAASTSQSNNTVSLNSEE438 pKa = 4.33DD439 pKa = 3.9SSAPTLSSSSPTDD452 pKa = 3.32GAGSVAVTSNIVLTFSEE469 pKa = 4.8TVDD472 pKa = 3.56VEE474 pKa = 4.3SGDD477 pKa = 3.49IVLYY481 pKa = 10.57KK482 pKa = 10.58ADD484 pKa = 3.66GTVVEE489 pKa = 4.8TFSLPSAKK497 pKa = 9.0VTGTGSTTITINPTANLSSSTDD519 pKa = 3.43YY520 pKa = 11.04YY521 pKa = 11.22LQIAATAFDD530 pKa = 4.48DD531 pKa = 4.41SASNSYY537 pKa = 11.13AGITDD542 pKa = 3.63TTTLNFTTVGSDD554 pKa = 2.92PTTNKK559 pKa = 9.5TSPLQSKK566 pKa = 7.38QQ567 pKa = 3.12

Molecular weight: 57.93 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D1KDM2|D1KDM2_9GAMM DUF4396 domain-containing protein OS=uncultured SUP05 cluster bacterium OX=655186 GN=Sup05_0638 PE=4 SV=1
MM1 pKa = 6.99AQVVKK6 pKa = 10.61RR7 pKa = 11.84KK8 pKa = 6.54PTSPGRR14 pKa = 11.84RR15 pKa = 11.84FVVSIVDD22 pKa = 3.49KK23 pKa = 10.48DD24 pKa = 3.63LHH26 pKa = 7.05KK27 pKa = 10.53GAPYY31 pKa = 10.82APLTEE36 pKa = 4.29SKK38 pKa = 10.93NRR40 pKa = 11.84ISGRR44 pKa = 11.84NNRR47 pKa = 11.84GKK49 pKa = 8.86ITVRR53 pKa = 11.84HH54 pKa = 6.09KK55 pKa = 11.19GGGHH59 pKa = 5.21KK60 pKa = 9.8RR61 pKa = 11.84RR62 pKa = 11.84YY63 pKa = 7.88RR64 pKa = 11.84TIDD67 pKa = 3.41FKK69 pKa = 11.0RR70 pKa = 11.84IKK72 pKa = 10.41DD73 pKa = 4.34DD74 pKa = 3.2IPATVEE80 pKa = 3.74RR81 pKa = 11.84LEE83 pKa = 4.03YY84 pKa = 10.85DD85 pKa = 3.48PNRR88 pKa = 11.84SANIALVLYY97 pKa = 10.67ADD99 pKa = 4.34GEE101 pKa = 4.34RR102 pKa = 11.84TYY104 pKa = 10.63IIAPNGLKK112 pKa = 10.73VGDD115 pKa = 4.03TVVSGTSVAIQAGNVMPCANIPLGSVIHH143 pKa = 6.55CIEE146 pKa = 4.45LKK148 pKa = 9.83PGKK151 pKa = 8.83GAQMARR157 pKa = 11.84SAGTSAQLIAKK168 pKa = 8.31EE169 pKa = 4.17GTYY172 pKa = 9.08VTLRR176 pKa = 11.84LRR178 pKa = 11.84SGEE181 pKa = 3.97VRR183 pKa = 11.84KK184 pKa = 10.26VLAEE188 pKa = 3.87CRR190 pKa = 11.84ATIGEE195 pKa = 4.22VSKK198 pKa = 11.08KK199 pKa = 7.95EE200 pKa = 3.6HH201 pKa = 6.15SLRR204 pKa = 11.84KK205 pKa = 9.46LGKK208 pKa = 10.11AGATRR213 pKa = 11.84WRR215 pKa = 11.84GVRR218 pKa = 11.84PTVRR222 pKa = 11.84GVVMNPVDD230 pKa = 3.77HH231 pKa = 6.67PHH233 pKa = 6.77GGGEE237 pKa = 4.38GKK239 pKa = 9.02TSGGRR244 pKa = 11.84HH245 pKa = 5.43PVSPWGTPTKK255 pKa = 10.39GYY257 pKa = 7.85KK258 pKa = 8.71TRR260 pKa = 11.84SNKK263 pKa = 8.79RR264 pKa = 11.84TDD266 pKa = 3.07KK267 pKa = 11.11LIVRR271 pKa = 11.84RR272 pKa = 11.84RR273 pKa = 11.84NKK275 pKa = 10.26

Molecular weight: 30.03 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1283

0

1283

338736

67

1499

264.0

29.41

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.463 ± 0.069

1.007 ± 0.028

6.085 ± 0.055

5.911 ± 0.055

4.267 ± 0.049

6.806 ± 0.064

2.232 ± 0.038

7.615 ± 0.057

7.143 ± 0.075

9.911 ± 0.085

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.712 ± 0.04

4.94 ± 0.053

3.388 ± 0.04

4.013 ± 0.05

3.757 ± 0.047

6.724 ± 0.059

5.313 ± 0.047

6.719 ± 0.057

0.986 ± 0.028

3.008 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski