Amino acid dipepetide frequency for uncultured SUP05 cluster bacterium

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.555AlaAla: 4.555 ± 0.166
0.847AlaCys: 0.847 ± 0.055
4.278AlaAsp: 4.278 ± 0.117
3.805AlaGlu: 3.805 ± 0.116
3.112AlaPhe: 3.112 ± 0.096
5.172AlaGly: 5.172 ± 0.133
1.671AlaHis: 1.671 ± 0.079
5.907AlaIle: 5.907 ± 0.15
6.031AlaLys: 6.031 ± 0.151
8.414AlaLeu: 8.414 ± 0.171
2.32AlaMet: 2.32 ± 0.099
3.54AlaAsn: 3.54 ± 0.103
2.196AlaPro: 2.196 ± 0.091
3.516AlaGln: 3.516 ± 0.118
2.796AlaArg: 2.796 ± 0.108
4.741AlaSer: 4.741 ± 0.125
4.0AlaThr: 4.0 ± 0.119
4.782AlaVal: 4.782 ± 0.129
0.714AlaTrp: 0.714 ± 0.05
2.226AlaTyr: 2.226 ± 0.079
0.0AlaXaa: 0.0 ± 0.0
Cys
0.776CysAla: 0.776 ± 0.051
0.124CysCys: 0.124 ± 0.021
0.673CysAsp: 0.673 ± 0.04
0.564CysGlu: 0.564 ± 0.047
0.372CysPhe: 0.372 ± 0.031
0.933CysGly: 0.933 ± 0.058
0.345CysHis: 0.345 ± 0.041
0.649CysIle: 0.649 ± 0.051
0.528CysLys: 0.528 ± 0.04
0.886CysLeu: 0.886 ± 0.051
0.236CysMet: 0.236 ± 0.029
0.475CysAsn: 0.475 ± 0.042
0.614CysPro: 0.614 ± 0.046
0.387CysGln: 0.387 ± 0.034
0.354CysArg: 0.354 ± 0.036
0.629CysSer: 0.629 ± 0.047
0.502CysThr: 0.502 ± 0.04
0.717CysVal: 0.717 ± 0.049
0.059CysTrp: 0.059 ± 0.014
0.245CysTyr: 0.245 ± 0.03
0.0CysXaa: 0.0 ± 0.0
Asp
4.381AspAla: 4.381 ± 0.121
0.582AspCys: 0.582 ± 0.046
3.646AspAsp: 3.646 ± 0.105
4.473AspGlu: 4.473 ± 0.123
2.999AspPhe: 2.999 ± 0.09
3.835AspGly: 3.835 ± 0.114
1.19AspHis: 1.19 ± 0.062
4.526AspIle: 4.526 ± 0.135
4.526AspLys: 4.526 ± 0.125
6.099AspLeu: 6.099 ± 0.16
1.579AspMet: 1.579 ± 0.056
2.926AspAsn: 2.926 ± 0.099
1.686AspPro: 1.686 ± 0.074
2.595AspGln: 2.595 ± 0.087
2.356AspArg: 2.356 ± 0.087
3.498AspSer: 3.498 ± 0.096
2.926AspThr: 2.926 ± 0.099
4.03AspVal: 4.03 ± 0.144
0.794AspTrp: 0.794 ± 0.045
2.205AspTyr: 2.205 ± 0.077
0.0AspXaa: 0.0 ± 0.0
Glu
4.384GluAla: 4.384 ± 0.144
0.517GluCys: 0.517 ± 0.042
3.156GluAsp: 3.156 ± 0.107
3.262GluGlu: 3.262 ± 0.117
2.778GluPhe: 2.778 ± 0.093
3.475GluGly: 3.475 ± 0.124
1.337GluHis: 1.337 ± 0.067
4.791GluIle: 4.791 ± 0.124
4.706GluLys: 4.706 ± 0.12
6.138GluLeu: 6.138 ± 0.152
1.733GluMet: 1.733 ± 0.07
2.751GluAsn: 2.751 ± 0.091
1.479GluPro: 1.479 ± 0.068
2.988GluGln: 2.988 ± 0.107
2.506GluArg: 2.506 ± 0.102
3.649GluSer: 3.649 ± 0.102
2.616GluThr: 2.616 ± 0.103
4.502GluVal: 4.502 ± 0.12
0.487GluTrp: 0.487 ± 0.044
1.854GluTyr: 1.854 ± 0.068
0.0GluXaa: 0.0 ± 0.0
Phe
3.053PheAla: 3.053 ± 0.099
0.517PheCys: 0.517 ± 0.038
3.466PheAsp: 3.466 ± 0.11
2.734PheGlu: 2.734 ± 0.1
1.96PhePhe: 1.96 ± 0.088
3.053PheGly: 3.053 ± 0.12
0.824PheHis: 0.824 ± 0.054
3.07PheIle: 3.07 ± 0.108
2.828PheLys: 2.828 ± 0.096
3.702PheLeu: 3.702 ± 0.117
1.098PheMet: 1.098 ± 0.06
2.306PheAsn: 2.306 ± 0.092
1.243PhePro: 1.243 ± 0.057
1.08PheGln: 1.08 ± 0.048
1.308PheArg: 1.308 ± 0.07
3.495PheSer: 3.495 ± 0.105
2.134PheThr: 2.134 ± 0.09
2.799PheVal: 2.799 ± 0.103
0.525PheTrp: 0.525 ± 0.043
1.473PheTyr: 1.473 ± 0.068
0.0PheXaa: 0.0 ± 0.0
Gly
5.293GlyAla: 5.293 ± 0.174
0.883GlyCys: 0.883 ± 0.062
3.737GlyAsp: 3.737 ± 0.115
4.171GlyGlu: 4.171 ± 0.108
3.457GlyPhe: 3.457 ± 0.101
4.93GlyGly: 4.93 ± 0.154
1.541GlyHis: 1.541 ± 0.07
5.193GlyIle: 5.193 ± 0.12
4.995GlyLys: 4.995 ± 0.123
6.681GlyLeu: 6.681 ± 0.163
2.016GlyMet: 2.016 ± 0.09
2.716GlyAsn: 2.716 ± 0.094
1.52GlyPro: 1.52 ± 0.079
2.205GlyGln: 2.205 ± 0.084
2.684GlyArg: 2.684 ± 0.1
4.245GlySer: 4.245 ± 0.13
3.262GlyThr: 3.262 ± 0.109
5.367GlyVal: 5.367 ± 0.144
0.8GlyTrp: 0.8 ± 0.057
2.362GlyTyr: 2.362 ± 0.102
0.0GlyXaa: 0.0 ± 0.0
His
1.574HisAla: 1.574 ± 0.066
0.325HisCys: 0.325 ± 0.032
0.989HisAsp: 0.989 ± 0.057
1.057HisGlu: 1.057 ± 0.056
0.939HisPhe: 0.939 ± 0.048
1.538HisGly: 1.538 ± 0.077
0.815HisHis: 0.815 ± 0.05
1.485HisIle: 1.485 ± 0.069
1.181HisLys: 1.181 ± 0.059
2.456HisLeu: 2.456 ± 0.086
0.54HisMet: 0.54 ± 0.037
1.066HisAsn: 1.066 ± 0.057
1.234HisPro: 1.234 ± 0.064
1.237HisGln: 1.237 ± 0.059
0.986HisArg: 0.986 ± 0.054
1.447HisSer: 1.447 ± 0.069
1.142HisThr: 1.142 ± 0.064
1.181HisVal: 1.181 ± 0.061
0.304HisTrp: 0.304 ± 0.028
0.824HisTyr: 0.824 ± 0.046
0.0HisXaa: 0.0 ± 0.0
Ile
6.353IleAla: 6.353 ± 0.141
0.673IleCys: 0.673 ± 0.051
5.562IleAsp: 5.562 ± 0.149
5.284IleGlu: 5.284 ± 0.13
2.766IlePhe: 2.766 ± 0.103
5.287IleGly: 5.287 ± 0.122
1.618IleHis: 1.618 ± 0.062
5.281IleIle: 5.281 ± 0.127
5.727IleLys: 5.727 ± 0.132
6.48IleLeu: 6.48 ± 0.139
1.603IleMet: 1.603 ± 0.065
4.446IleAsn: 4.446 ± 0.126
2.707IlePro: 2.707 ± 0.098
2.551IleGln: 2.551 ± 0.084
2.728IleArg: 2.728 ± 0.083
5.388IleSer: 5.388 ± 0.147
4.399IleThr: 4.399 ± 0.133
4.768IleVal: 4.768 ± 0.134
0.564IleTrp: 0.564 ± 0.036
1.969IleTyr: 1.969 ± 0.078
0.0IleXaa: 0.0 ± 0.0
Lys
5.281LysAla: 5.281 ± 0.14
0.44LysCys: 0.44 ± 0.035
4.573LysAsp: 4.573 ± 0.116
4.062LysGlu: 4.062 ± 0.128
2.338LysPhe: 2.338 ± 0.086
4.405LysGly: 4.405 ± 0.128
1.695LysHis: 1.695 ± 0.071
5.674LysIle: 5.674 ± 0.153
5.585LysLys: 5.585 ± 0.173
6.229LysLeu: 6.229 ± 0.139
2.069LysMet: 2.069 ± 0.088
4.044LysAsn: 4.044 ± 0.124
2.512LysPro: 2.512 ± 0.115
3.481LysGln: 3.481 ± 0.099
2.94LysArg: 2.94 ± 0.095
4.47LysSer: 4.47 ± 0.128
4.481LysThr: 4.481 ± 0.102
4.664LysVal: 4.664 ± 0.124
0.52LysTrp: 0.52 ± 0.037
1.966LysTyr: 1.966 ± 0.07
0.0LysXaa: 0.0 ± 0.0
Leu
7.451LeuAla: 7.451 ± 0.17
0.868LeuCys: 0.868 ± 0.046
6.35LeuAsp: 6.35 ± 0.163
6.052LeuGlu: 6.052 ± 0.15
4.098LeuPhe: 4.098 ± 0.134
6.935LeuGly: 6.935 ± 0.163
1.73LeuHis: 1.73 ± 0.074
7.36LeuIle: 7.36 ± 0.181
7.135LeuLys: 7.135 ± 0.143
9.825LeuLeu: 9.825 ± 0.217
2.675LeuMet: 2.675 ± 0.102
4.983LeuAsn: 4.983 ± 0.149
3.838LeuPro: 3.838 ± 0.097
2.813LeuGln: 2.813 ± 0.087
3.475LeuArg: 3.475 ± 0.099
8.561LeuSer: 8.561 ± 0.187
5.317LeuThr: 5.317 ± 0.131
6.341LeuVal: 6.341 ± 0.144
0.865LeuTrp: 0.865 ± 0.062
2.441LeuTyr: 2.441 ± 0.106
0.0LeuXaa: 0.0 ± 0.0
Met
2.131MetAla: 2.131 ± 0.082
0.218MetCys: 0.218 ± 0.025
1.562MetAsp: 1.562 ± 0.07
1.326MetGlu: 1.326 ± 0.085
0.93MetPhe: 0.93 ± 0.051
2.034MetGly: 2.034 ± 0.091
0.596MetHis: 0.596 ± 0.043
1.854MetIle: 1.854 ± 0.077
1.86MetLys: 1.86 ± 0.07
2.506MetLeu: 2.506 ± 0.087
0.835MetMet: 0.835 ± 0.059
1.355MetAsn: 1.355 ± 0.064
1.228MetPro: 1.228 ± 0.062
1.204MetGln: 1.204 ± 0.057
1.151MetArg: 1.151 ± 0.056
2.164MetSer: 2.164 ± 0.075
1.467MetThr: 1.467 ± 0.073
1.951MetVal: 1.951 ± 0.084
0.192MetTrp: 0.192 ± 0.022
0.558MetTyr: 0.558 ± 0.044
0.0MetXaa: 0.0 ± 0.0
Asn
3.838AsnAla: 3.838 ± 0.103
0.446AsnCys: 0.446 ± 0.035
2.657AsnAsp: 2.657 ± 0.09
2.816AsnGlu: 2.816 ± 0.093
1.978AsnPhe: 1.978 ± 0.077
2.967AsnGly: 2.967 ± 0.105
1.157AsnHis: 1.157 ± 0.058
4.275AsnIle: 4.275 ± 0.116
3.705AsnLys: 3.705 ± 0.117
4.242AsnLeu: 4.242 ± 0.125
1.063AsnMet: 1.063 ± 0.058
2.923AsnAsn: 2.923 ± 0.148
2.297AsnPro: 2.297 ± 0.08
2.4AsnGln: 2.4 ± 0.091
1.83AsnArg: 1.83 ± 0.073
2.689AsnSer: 2.689 ± 0.117
3.1AsnThr: 3.1 ± 0.141
2.834AsnVal: 2.834 ± 0.092
0.561AsnTrp: 0.561 ± 0.044
1.618AsnTyr: 1.618 ± 0.071
0.0AsnXaa: 0.0 ± 0.0
Pro
2.185ProAla: 2.185 ± 0.08
0.41ProCys: 0.41 ± 0.037
1.904ProAsp: 1.904 ± 0.075
2.329ProGlu: 2.329 ± 0.091
1.606ProPhe: 1.606 ± 0.073
2.067ProGly: 2.067 ± 0.074
0.735ProHis: 0.735 ± 0.045
2.737ProIle: 2.737 ± 0.092
2.486ProLys: 2.486 ± 0.092
3.318ProLeu: 3.318 ± 0.095
1.148ProMet: 1.148 ± 0.06
1.798ProAsn: 1.798 ± 0.084
1.045ProPro: 1.045 ± 0.057
1.125ProGln: 1.125 ± 0.054
0.968ProArg: 0.968 ± 0.052
2.303ProSer: 2.303 ± 0.08
1.819ProThr: 1.819 ± 0.07
2.309ProVal: 2.309 ± 0.081
0.469ProTrp: 0.469 ± 0.044
1.122ProTyr: 1.122 ± 0.066
0.0ProXaa: 0.0 ± 0.0
Gln
3.54GlnAla: 3.54 ± 0.109
0.342GlnCys: 0.342 ± 0.035
2.335GlnAsp: 2.335 ± 0.087
2.002GlnGlu: 2.002 ± 0.085
1.42GlnPhe: 1.42 ± 0.069
2.654GlnGly: 2.654 ± 0.081
1.013GlnHis: 1.013 ± 0.059
3.126GlnIle: 3.126 ± 0.096
2.893GlnLys: 2.893 ± 0.097
4.059GlnLeu: 4.059 ± 0.11
1.075GlnMet: 1.075 ± 0.057
1.804GlnAsn: 1.804 ± 0.074
1.033GlnPro: 1.033 ± 0.05
1.733GlnGln: 1.733 ± 0.085
1.408GlnArg: 1.408 ± 0.071
2.825GlnSer: 2.825 ± 0.095
2.344GlnThr: 2.344 ± 0.082
2.94GlnVal: 2.94 ± 0.085
0.36GlnTrp: 0.36 ± 0.036
1.225GlnTyr: 1.225 ± 0.066
0.0GlnXaa: 0.0 ± 0.0
Arg
2.743ArgAla: 2.743 ± 0.102
0.325ArgCys: 0.325 ± 0.034
2.037ArgAsp: 2.037 ± 0.081
2.279ArgGlu: 2.279 ± 0.097
1.792ArgPhe: 1.792 ± 0.072
2.406ArgGly: 2.406 ± 0.093
0.838ArgHis: 0.838 ± 0.043
3.12ArgIle: 3.12 ± 0.092
2.468ArgLys: 2.468 ± 0.088
4.009ArgLeu: 4.009 ± 0.113
1.101ArgMet: 1.101 ± 0.062
1.609ArgAsn: 1.609 ± 0.071
1.181ArgPro: 1.181 ± 0.066
1.455ArgGln: 1.455 ± 0.07
1.621ArgArg: 1.621 ± 0.077
2.309ArgSer: 2.309 ± 0.086
1.703ArgThr: 1.703 ± 0.07
2.651ArgVal: 2.651 ± 0.097
0.443ArgTrp: 0.443 ± 0.038
1.479ArgTyr: 1.479 ± 0.061
0.0ArgXaa: 0.0 ± 0.0
Ser
5.379SerAla: 5.379 ± 0.134
0.661SerCys: 0.661 ± 0.041
3.935SerAsp: 3.935 ± 0.107
3.805SerGlu: 3.805 ± 0.104
2.979SerPhe: 2.979 ± 0.095
5.004SerGly: 5.004 ± 0.142
1.414SerHis: 1.414 ± 0.066
5.464SerIle: 5.464 ± 0.133
4.629SerLys: 4.629 ± 0.119
6.713SerLeu: 6.713 ± 0.148
1.816SerMet: 1.816 ± 0.071
3.277SerAsn: 3.277 ± 0.111
1.943SerPro: 1.943 ± 0.064
2.604SerGln: 2.604 ± 0.097
2.462SerArg: 2.462 ± 0.097
4.626SerSer: 4.626 ± 0.152
3.534SerThr: 3.534 ± 0.118
4.599SerVal: 4.599 ± 0.126
0.59SerTrp: 0.59 ± 0.041
1.81SerTyr: 1.81 ± 0.074
0.0SerXaa: 0.0 ± 0.0
Thr
3.661ThrAla: 3.661 ± 0.121
0.555ThrCys: 0.555 ± 0.045
3.236ThrAsp: 3.236 ± 0.118
2.436ThrGlu: 2.436 ± 0.079
1.99ThrPhe: 1.99 ± 0.076
4.074ThrGly: 4.074 ± 0.1
1.429ThrHis: 1.429 ± 0.07
3.974ThrIle: 3.974 ± 0.116
3.315ThrLys: 3.315 ± 0.102
6.067ThrLeu: 6.067 ± 0.123
1.334ThrMet: 1.334 ± 0.06
2.601ThrAsn: 2.601 ± 0.086
2.489ThrPro: 2.489 ± 0.083
2.577ThrGln: 2.577 ± 0.093
1.839ThrArg: 1.839 ± 0.079
3.02ThrSer: 3.02 ± 0.116
3.174ThrThr: 3.174 ± 0.112
3.315ThrVal: 3.315 ± 0.121
0.514ThrTrp: 0.514 ± 0.036
1.529ThrTyr: 1.529 ± 0.072
0.0ThrXaa: 0.0 ± 0.0
Val
5.184ValAla: 5.184 ± 0.151
0.765ValCys: 0.765 ± 0.049
4.582ValAsp: 4.582 ± 0.116
4.579ValGlu: 4.579 ± 0.13
3.174ValPhe: 3.174 ± 0.1
4.629ValGly: 4.629 ± 0.132
1.199ValHis: 1.199 ± 0.062
5.219ValIle: 5.219 ± 0.131
4.52ValLys: 4.52 ± 0.122
6.657ValLeu: 6.657 ± 0.137
1.881ValMet: 1.881 ± 0.08
2.896ValAsn: 2.896 ± 0.104
2.161ValPro: 2.161 ± 0.074
1.913ValGln: 1.913 ± 0.078
2.379ValArg: 2.379 ± 0.086
4.765ValSer: 4.765 ± 0.118
3.283ValThr: 3.283 ± 0.103
5.143ValVal: 5.143 ± 0.134
0.54ValTrp: 0.54 ± 0.044
1.724ValTyr: 1.724 ± 0.073
0.0ValXaa: 0.0 ± 0.0
Trp
0.596TrpAla: 0.596 ± 0.042
0.186TrpCys: 0.186 ± 0.023
0.567TrpAsp: 0.567 ± 0.045
0.528TrpGlu: 0.528 ± 0.037
0.452TrpPhe: 0.452 ± 0.037
0.7TrpGly: 0.7 ± 0.055
0.348TrpHis: 0.348 ± 0.033
0.679TrpIle: 0.679 ± 0.049
0.549TrpLys: 0.549 ± 0.04
1.075TrpLeu: 1.075 ± 0.064
0.339TrpMet: 0.339 ± 0.03
0.469TrpAsn: 0.469 ± 0.041
0.213TrpPro: 0.213 ± 0.026
0.561TrpGln: 0.561 ± 0.04
0.434TrpArg: 0.434 ± 0.038
0.561TrpSer: 0.561 ± 0.039
0.419TrpThr: 0.419 ± 0.035
0.682TrpVal: 0.682 ± 0.049
0.165TrpTrp: 0.165 ± 0.026
0.339TrpTyr: 0.339 ± 0.039
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.27TyrAla: 2.27 ± 0.087
0.387TyrCys: 0.387 ± 0.033
1.606TyrAsp: 1.606 ± 0.061
1.544TyrGlu: 1.544 ± 0.069
1.529TyrPhe: 1.529 ± 0.069
1.963TyrGly: 1.963 ± 0.085
0.824TyrHis: 0.824 ± 0.047
1.768TyrIle: 1.768 ± 0.073
1.762TyrLys: 1.762 ± 0.075
3.557TyrLeu: 3.557 ± 0.124
0.658TyrMet: 0.658 ± 0.037
1.411TyrAsn: 1.411 ± 0.063
1.264TyrPro: 1.264 ± 0.062
1.706TyrGln: 1.706 ± 0.073
1.343TyrArg: 1.343 ± 0.064
1.86TyrSer: 1.86 ± 0.083
1.506TyrThr: 1.506 ± 0.065
1.615TyrVal: 1.615 ± 0.065
0.396TyrTrp: 0.396 ± 0.037
1.107TyrTyr: 1.107 ± 0.065
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 1283 proteins (338736 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski