Geitlerinema sp. PCC 7407

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Cyanobacteria/Melainabacteria group; Cyanobacteria; Oscillatoriophycideae; Oscillatoriales; Coleofasciculaceae; Geitlerinema; unclassified Geitlerinema

Average proteome isoelectric point is 6.11

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3813 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|K9SE77|K9SE77_9CYAN Thiamine-phosphate synthase OS=Geitlerinema sp. PCC 7407 OX=1173025 GN=thiE PE=3 SV=1
MM1 pKa = 7.89AFRR4 pKa = 11.84LLLFRR9 pKa = 11.84RR10 pKa = 11.84RR11 pKa = 11.84FMTLHH16 pKa = 6.88PSDD19 pKa = 4.78SNTPTSSNSQDD30 pKa = 3.64PEE32 pKa = 6.11AIDD35 pKa = 4.13PKK37 pKa = 11.19ASGDD41 pKa = 4.72DD42 pKa = 3.52FDD44 pKa = 6.55DD45 pKa = 5.0YY46 pKa = 11.97NDD48 pKa = 4.46PTCQWTPYY56 pKa = 8.72PTDD59 pKa = 3.7EE60 pKa = 4.8DD61 pKa = 4.26CGDD64 pKa = 4.02GLSCPPCSS72 pKa = 3.62

Molecular weight: 8.05 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|K9SDA4|K9SDA4_9CYAN Multi-sensor hybrid histidine kinase OS=Geitlerinema sp. PCC 7407 OX=1173025 GN=GEI7407_3711 PE=4 SV=1
MM1 pKa = 6.61TQRR4 pKa = 11.84TLGGTCRR11 pKa = 11.84KK12 pKa = 9.61RR13 pKa = 11.84KK14 pKa = 7.61RR15 pKa = 11.84TSGFRR20 pKa = 11.84ARR22 pKa = 11.84MRR24 pKa = 11.84TKK26 pKa = 10.49NGQNVIKK33 pKa = 10.53ARR35 pKa = 11.84RR36 pKa = 11.84NKK38 pKa = 9.33GRR40 pKa = 11.84ARR42 pKa = 11.84LAVV45 pKa = 3.42

Molecular weight: 5.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3813

0

3813

1300425

30

4135

341.1

37.6

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.244 ± 0.047

0.968 ± 0.013

5.172 ± 0.039

6.146 ± 0.047

3.556 ± 0.025

7.279 ± 0.039

1.927 ± 0.019

5.201 ± 0.029

2.923 ± 0.031

11.505 ± 0.054

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.851 ± 0.02

2.909 ± 0.038

5.593 ± 0.044

5.51 ± 0.041

6.524 ± 0.037

6.159 ± 0.036

5.326 ± 0.036

6.975 ± 0.035

1.554 ± 0.02

2.678 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski