Megamonas hypermegale

Taxonomy: cellular organisms;

Average proteome isoelectric point is 6.56

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2073 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A239T8R0|A0A239T8R0_9FIRM NADPH azoreductase OS=Megamonas hypermegale OX=158847 GN=azr PE=4 SV=1
MM1 pKa = 7.19EE2 pKa = 5.42RR3 pKa = 11.84EE4 pKa = 3.91EE5 pKa = 4.25VLLKK9 pKa = 10.32IAQLISNNDD18 pKa = 2.9SDD20 pKa = 5.08VIIEE24 pKa = 4.06VEE26 pKa = 4.06EE27 pKa = 5.13CIDD30 pKa = 5.25DD31 pKa = 3.62IQKK34 pKa = 10.48YY35 pKa = 8.64VATHH39 pKa = 5.9LEE41 pKa = 4.26EE42 pKa = 5.48YY43 pKa = 9.83EE44 pKa = 4.13DD45 pKa = 4.6RR46 pKa = 11.84SMTLEE51 pKa = 4.77DD52 pKa = 5.29DD53 pKa = 4.69SEE55 pKa = 5.44EE56 pKa = 4.04DD57 pKa = 3.88LQWLGMVNCLIRR69 pKa = 11.84NNYY72 pKa = 9.0VYY74 pKa = 9.74EE75 pKa = 4.22TDD77 pKa = 4.57DD78 pKa = 3.37NVQITEE84 pKa = 4.56FGWEE88 pKa = 4.19LKK90 pKa = 10.22NLCGFKK96 pKa = 10.36EE97 pKa = 4.22YY98 pKa = 11.04GLSLDD103 pKa = 4.49SKK105 pKa = 10.94FLDD108 pKa = 2.9VDD110 pKa = 4.03YY111 pKa = 11.39GVDD114 pKa = 3.17RR115 pKa = 11.84YY116 pKa = 11.02DD117 pKa = 3.87INEE120 pKa = 4.42DD121 pKa = 3.64DD122 pKa = 4.97EE123 pKa = 6.04DD124 pKa = 4.39VFDD127 pKa = 4.49YY128 pKa = 11.36DD129 pKa = 3.82EE130 pKa = 5.16EE131 pKa = 4.59EE132 pKa = 5.78DD133 pKa = 5.58FIEE136 pKa = 5.31DD137 pKa = 3.69DD138 pKa = 6.3NIIGWCKK145 pKa = 10.52YY146 pKa = 10.63LDD148 pKa = 3.78NKK150 pKa = 9.1WADD153 pKa = 3.63EE154 pKa = 4.09NVCIGMININSDD166 pKa = 3.28SYY168 pKa = 11.38IIFPIDD174 pKa = 3.51CDD176 pKa = 3.73VLEE179 pKa = 4.49EE180 pKa = 4.97LEE182 pKa = 5.0DD183 pKa = 3.86LADD186 pKa = 4.22SIDD189 pKa = 3.46RR190 pKa = 11.84HH191 pKa = 5.74ISSVYY196 pKa = 10.22

Molecular weight: 23.08 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A239TK73|A0A239TK73_9FIRM Uncharacterized protein OS=Megamonas hypermegale OX=158847 GN=SAMEA4364220_00887 PE=4 SV=1
MM1 pKa = 7.49LKK3 pKa = 10.65DD4 pKa = 3.97KK5 pKa = 10.68IKK7 pKa = 9.64KK8 pKa = 7.48TVGILTMACFLATPIGYY25 pKa = 9.75VISASTTEE33 pKa = 4.47AAPPPPPPRR42 pKa = 11.84HH43 pKa = 6.34RR44 pKa = 11.84IEE46 pKa = 4.68APPPSHH52 pKa = 7.02HH53 pKa = 7.13KK54 pKa = 10.0GPSWKK59 pKa = 8.81RR60 pKa = 11.84TPPPPQHH67 pKa = 6.36RR68 pKa = 11.84WKK70 pKa = 10.39DD71 pKa = 3.36DD72 pKa = 2.9HH73 pKa = 6.93RR74 pKa = 11.84RR75 pKa = 11.84PDD77 pKa = 3.9APPPPPRR84 pKa = 11.84HH85 pKa = 4.95HH86 pKa = 7.71RR87 pKa = 11.84YY88 pKa = 9.39RR89 pKa = 5.14

Molecular weight: 10.11 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2073

0

2073

651883

30

3257

314.5

35.25

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.443 ± 0.066

1.306 ± 0.023

5.721 ± 0.051

6.19 ± 0.052

4.328 ± 0.047

6.576 ± 0.052

1.829 ± 0.024

8.798 ± 0.065

7.728 ± 0.06

9.134 ± 0.066

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.955 ± 0.027

5.508 ± 0.058

3.387 ± 0.033

3.254 ± 0.028

3.669 ± 0.037

5.46 ± 0.039

5.335 ± 0.054

6.632 ± 0.047

0.834 ± 0.02

3.913 ± 0.037
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski