Larkinella punicea
Average proteome isoelectric point is 6.9
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6294 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A368JVB2|A0A368JVB2_9BACT AraC family transcriptional regulator OS=Larkinella punicea OX=2315727 GN=DUE52_06505 PE=4 SV=1
MM1 pKa = 7.43 SLLAVMGMLVSIPPAYY17 pKa = 10.05 AQSCYY22 pKa = 11.15 APISGTAVQVGGVISATVCAGPLCLGTGTYY52 pKa = 10.75 NEE54 pKa = 4.28 LQKK57 pKa = 9.83 ITDD60 pKa = 3.47 NSLEE64 pKa = 4.04 TFANINTTIAAQIAQGISVKK84 pKa = 9.38 RR85 pKa = 11.84 TNGTYY90 pKa = 9.57 TGEE93 pKa = 4.11 NVVGFILSRR102 pKa = 11.84 SALVDD107 pKa = 3.18 VSVQSSVTIRR117 pKa = 11.84 TYY119 pKa = 11.5 LNGNPVSAAQPLNALVSGSALGSVEE144 pKa = 4.33 KK145 pKa = 10.13 MYY147 pKa = 10.11 ATFTTNQEE155 pKa = 3.73 FDD157 pKa = 3.54 EE158 pKa = 4.42 VRR160 pKa = 11.84 LNTSLLSAEE169 pKa = 4.28 VFSNLRR175 pKa = 11.84 IFAAVAFPTSCSTPVSTTACNDD197 pKa = 3.7 YY198 pKa = 10.9 ISGLGTDD205 pKa = 3.15 ATYY208 pKa = 10.87 YY209 pKa = 11.22 GSVTCAACSLTDD221 pKa = 3.65 RR222 pKa = 11.84 ANLIDD227 pKa = 4.52 GNNANYY233 pKa = 10.61 AVLTPTAGVASEE245 pKa = 4.34 ISVSVIDD252 pKa = 3.67 TKK254 pKa = 11.13 KK255 pKa = 10.29 KK256 pKa = 10.12 YY257 pKa = 9.94 KK258 pKa = 10.7 AGDD261 pKa = 3.22 RR262 pKa = 11.84 AGFVIAKK269 pKa = 8.84 NQVNALLALNALSTITIEE287 pKa = 4.22 TYY289 pKa = 11.0 LFGTLQEE296 pKa = 4.56 AKK298 pKa = 10.73 SPGDD302 pKa = 3.35 PTNVVGLSLLAGSNTIPKK320 pKa = 9.59 QKK322 pKa = 10.65 LGFTTTLDD330 pKa = 3.62 FNEE333 pKa = 3.4 VRR335 pKa = 11.84 IRR337 pKa = 11.84 INSLATANVGAIRR350 pKa = 11.84 LYY352 pKa = 10.77 GAFVEE357 pKa = 5.2 ATTCTNCEE365 pKa = 4.02 TLLNNAGTGKK375 pKa = 10.56 YY376 pKa = 9.92 NGAIVTGFQSLGLPWTGVHH395 pKa = 6.68 GLGLHH400 pKa = 6.89 ALTNSGNAVTSTPTDD415 pKa = 3.28 DD416 pKa = 3.45 FATYY420 pKa = 9.18 TSVVGVLGAGLQFTVEE436 pKa = 3.85 NDD438 pKa = 3.54 GTDD441 pKa = 3.86 FTANTTFAGFHH452 pKa = 5.86 ISKK455 pKa = 8.79 TGSLIALGLLDD466 pKa = 5.87 AITIKK471 pKa = 10.69 VYY473 pKa = 11.1 NGTTLVDD480 pKa = 3.88 EE481 pKa = 4.57 STSASLLGASLISGTTTRR499 pKa = 11.84 SVVGFYY505 pKa = 10.49 PDD507 pKa = 3.14 AAFDD511 pKa = 5.01 RR512 pKa = 11.84 IQLIVNEE519 pKa = 4.63 GLLSADD525 pKa = 3.52 LAGDD529 pKa = 3.55 YY530 pKa = 10.72 LIYY533 pKa = 10.56 DD534 pKa = 3.95 AFVIEE539 pKa = 4.97 DD540 pKa = 3.76 ADD542 pKa = 4.03 GDD544 pKa = 4.42 GVPDD548 pKa = 4.06 CQDD551 pKa = 2.81 VCGTGTGNDD560 pKa = 3.95 LLDD563 pKa = 4.11 FDD565 pKa = 5.76 GDD567 pKa = 4.77 GIPDD571 pKa = 3.71 TCDD574 pKa = 3.65 PDD576 pKa = 5.03 DD577 pKa = 5.85 DD578 pKa = 5.14 NDD580 pKa = 5.16 GIPDD584 pKa = 3.71 TVEE587 pKa = 3.94 NPEE590 pKa = 4.14 GDD592 pKa = 3.82 PNRR595 pKa = 11.84 DD596 pKa = 2.98 TDD598 pKa = 4.06 GDD600 pKa = 4.07 SSPDD604 pKa = 3.92 LRR606 pKa = 11.84 DD607 pKa = 3.5 LDD609 pKa = 4.27 SDD611 pKa = 3.78 NDD613 pKa = 4.74 GIPDD617 pKa = 3.89 SVEE620 pKa = 3.88 KK621 pKa = 11.09 GPDD624 pKa = 3.25 GNNPSNTDD632 pKa = 3.06 GTDD635 pKa = 3.15 VPDD638 pKa = 3.92 YY639 pKa = 10.81 RR640 pKa = 11.84 DD641 pKa = 3.8 PDD643 pKa = 3.78 SDD645 pKa = 3.91 NDD647 pKa = 4.73 GIPDD651 pKa = 3.68 SVEE654 pKa = 3.76 AGPNGDD660 pKa = 3.82 EE661 pKa = 4.61 PRR663 pKa = 11.84 NTDD666 pKa = 3.11 GDD668 pKa = 4.27 ALPDD672 pKa = 3.9 YY673 pKa = 11.2 LDD675 pKa = 5.06 LDD677 pKa = 4.06 SDD679 pKa = 3.94 NDD681 pKa = 4.73 GILDD685 pKa = 3.95 TVEE688 pKa = 4.63 KK689 pKa = 10.8 GSDD692 pKa = 3.41 GNNPTNSDD700 pKa = 3.85 TNTGSNPDD708 pKa = 3.62 GRR710 pKa = 11.84 PDD712 pKa = 4.83 YY713 pKa = 11.01 IDD715 pKa = 5.67 LDD717 pKa = 4.07 ADD719 pKa = 3.53 NDD721 pKa = 4.74 GINDD725 pKa = 3.8 VIEE728 pKa = 4.53 ANPNGSPTKK737 pKa = 10.36 DD738 pKa = 3.4 SNGDD742 pKa = 3.72 GIADD746 pKa = 4.11 GTPGATGIPASAGSGLTPPDD766 pKa = 3.38 TDD768 pKa = 4.54 GDD770 pKa = 4.2 TKK772 pKa = 11.21 PDD774 pKa = 3.64 FQDD777 pKa = 3.15 IDD779 pKa = 3.73 TDD781 pKa = 3.95 GDD783 pKa = 4.4 GIPDD787 pKa = 4.33 LYY789 pKa = 11.09 EE790 pKa = 4.74 SGIPNPNALDD800 pKa = 3.75 TNKK803 pKa = 10.9 DD804 pKa = 3.66 GIIDD808 pKa = 4.11 DD809 pKa = 4.93 ASEE812 pKa = 4.1 TDD814 pKa = 3.3 NDD816 pKa = 5.44 GIPTVNAPAAVVDD829 pKa = 4.59 GANGTYY835 pKa = 10.88 GDD837 pKa = 4.53 ANSPALPNNDD847 pKa = 2.17 GDD849 pKa = 4.31 AYY851 pKa = 10.21 PNYY854 pKa = 10.04 RR855 pKa = 11.84 DD856 pKa = 4.59 LDD858 pKa = 3.84 SDD860 pKa = 3.75 NDD862 pKa = 4.86 GITDD866 pKa = 3.88 TVEE869 pKa = 3.92 KK870 pKa = 10.57 GIDD873 pKa = 3.53 GANPTNTDD881 pKa = 2.79 GDD883 pKa = 4.53 LLPDD887 pKa = 3.94 YY888 pKa = 11.18 LDD890 pKa = 4.96 LDD892 pKa = 4.15 SDD894 pKa = 3.93 NDD896 pKa = 4.59 GINDD900 pKa = 3.5 VVEE903 pKa = 4.39 ATGSPTNDD911 pKa = 3.15 SNGDD915 pKa = 3.84 GIADD919 pKa = 4.11 GTPGATGIPSSAGSGVTPADD939 pKa = 3.84 ADD941 pKa = 3.76 NDD943 pKa = 3.75 GSPNYY948 pKa = 9.88 RR949 pKa = 11.84 DD950 pKa = 4.12 LDD952 pKa = 3.7 SDD954 pKa = 3.83 NDD956 pKa = 4.14 SKK958 pKa = 11.71 KK959 pKa = 10.71 DD960 pKa = 3.56 LYY962 pKa = 11.19 EE963 pKa = 4.8 SGIPNPASLDD973 pKa = 3.76 TNGDD977 pKa = 3.84 GVVDD981 pKa = 4.17 AADD984 pKa = 3.77 GTDD987 pKa = 3.45 NDD989 pKa = 5.84 GIPQTVDD996 pKa = 3.29 GEE998 pKa = 4.37 PNAYY1002 pKa = 9.73 GDD1004 pKa = 4.13 SGNPTLPNEE1013 pKa = 4.6 DD1014 pKa = 3.23 TDD1016 pKa = 4.08 EE1017 pKa = 4.65 NPDD1020 pKa = 3.91 YY1021 pKa = 10.73 IDD1023 pKa = 3.81 PAGVDD1028 pKa = 4.19 LYY1030 pKa = 9.47 PTLSMPDD1037 pKa = 3.39 RR1038 pKa = 11.84 LYY1040 pKa = 11.13 GASGGAKK1047 pKa = 8.42 NLKK1050 pKa = 8.04 VQVYY1054 pKa = 10.17 NLIPATNTNGTITLTISRR1072 pKa = 11.84 PSPFYY1077 pKa = 11.07 TMALSTAEE1085 pKa = 4.07 ALQDD1089 pKa = 3.23 QWILTTEE1096 pKa = 3.94 TLLFKK1101 pKa = 10.63 LVSKK1105 pKa = 9.73 TGVTIAGSSFNEE1117 pKa = 3.64 IEE1119 pKa = 4.27 MVLTASGSAAPGLANLNINILAGSGGEE1146 pKa = 4.1 TNTANNVASAQITIEE1161 pKa = 3.9
Molecular weight: 119.49 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.82
IPC2_protein 3.49
IPC_protein 3.567
Toseland 3.312
ProMoST 3.745
Dawson 3.579
Bjellqvist 3.732
Wikipedia 3.541
Rodwell 3.376
Grimsley 3.223
Solomon 3.579
Lehninger 3.528
Nozaki 3.681
DTASelect 3.999
Thurlkill 3.376
EMBOSS 3.554
Sillero 3.694
Patrickios 2.104
IPC_peptide 3.567
IPC2_peptide 3.668
IPC2.peptide.svr19 3.692
Protein with the highest isoelectric point:
>tr|A0A368JGN6|A0A368JGN6_9BACT 3-oxoacyl-[acyl-carrier-protein] synthase 3 OS=Larkinella punicea OX=2315727 GN=fabH PE=3 SV=1
MM1 pKa = 7.64 PKK3 pKa = 10.07 SLSFRR8 pKa = 11.84 SGLGSVLFWSVISAAFIGPGTVTTCAMAGSRR39 pKa = 11.84 FGLDD43 pKa = 3.69 LLWALTFSTIGTIVLQEE60 pKa = 3.5 AAARR64 pKa = 11.84 ITIASGLSLGQVVARR79 pKa = 11.84 SYY81 pKa = 10.1 GTGSRR86 pKa = 11.84 WIAGALLFSIVLGCAAYY103 pKa = 10.05 QAGNILGAVAGLSMILPVSPVWLTAGIGLVCIFLLWQGSTRR144 pKa = 11.84 LIANFLGVIVFVMGVAFAYY163 pKa = 10.18 VATRR167 pKa = 11.84 TDD169 pKa = 2.93 VSAGMFVKK177 pKa = 10.74 ALVVPVATADD187 pKa = 3.66 SFVLVIGLIGTTIVPYY203 pKa = 10.94 NLFLASGLGKK213 pKa = 10.22 GQSLAEE219 pKa = 4.01 MRR221 pKa = 11.84 WGIGLAVLIGGLISMAILVTGTLVTGEE248 pKa = 4.75 FSFQQVADD256 pKa = 3.84 ALANRR261 pKa = 11.84 VGGWSRR267 pKa = 11.84 RR268 pKa = 11.84 LFAFGLFAAGFTSALTAPMAAAVTSQSLLGWAGSSWRR305 pKa = 11.84 YY306 pKa = 8.62 RR307 pKa = 11.84 LVWLGVMGVGLMFGLMNARR326 pKa = 11.84 PVPVIIVVQAINGVLLPVVTIFLFRR351 pKa = 11.84 AVNDD355 pKa = 3.48 KK356 pKa = 11.05 KK357 pKa = 10.96 LIPEE361 pKa = 4.75 AYY363 pKa = 9.81 RR364 pKa = 11.84 NSGLQNLAMLVVVAVSLLFGGWNVWLAVQNWLKK397 pKa = 11.12
Molecular weight: 41.61 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.34
IPC2_protein 9.78
IPC_protein 10.628
Toseland 10.511
ProMoST 10.335
Dawson 10.672
Bjellqvist 10.423
Wikipedia 10.906
Rodwell 10.833
Grimsley 10.745
Solomon 10.76
Lehninger 10.716
Nozaki 10.511
DTASelect 10.409
Thurlkill 10.54
EMBOSS 10.921
Sillero 10.599
Patrickios 10.526
IPC_peptide 10.76
IPC2_peptide 9.575
IPC2.peptide.svr19 8.41
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6294
0
6294
2371370
21
2512
376.8
42.09
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.941 ± 0.028
0.717 ± 0.009
5.236 ± 0.024
5.51 ± 0.027
4.672 ± 0.021
7.223 ± 0.029
1.815 ± 0.016
5.862 ± 0.026
5.449 ± 0.028
10.058 ± 0.035
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.071 ± 0.015
4.731 ± 0.024
4.639 ± 0.022
4.271 ± 0.02
5.3 ± 0.024
6.13 ± 0.024
6.313 ± 0.032
6.913 ± 0.022
1.363 ± 0.011
3.786 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here