Symmachiella dynata
Average proteome isoelectric point is 6.08
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6115 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A517ZJ75|A0A517ZJ75_9PLAN PDZ domain-containing protein OS=Symmachiella dynata OX=2527995 GN=Mal52_10150 PE=4 SV=1
MM1 pKa = 7.64 FEE3 pKa = 4.05 LTAGGTYY10 pKa = 10.64 SLLIEE15 pKa = 4.6 GTLDD19 pKa = 3.32 DD20 pKa = 4.3 TPVTRR25 pKa = 11.84 PYY27 pKa = 11.17 AFQIEE32 pKa = 4.44 LLEE35 pKa = 4.39 LSSTSSLSATNSGSISASGDD55 pKa = 2.99 LHH57 pKa = 7.28 AFTFSATAGEE67 pKa = 4.51 NYY69 pKa = 10.02 FVDD72 pKa = 4.31 FADD75 pKa = 4.49 AATTDD80 pKa = 3.64 HH81 pKa = 6.44 YY82 pKa = 11.72 LSIRR86 pKa = 11.84 DD87 pKa = 3.53 AAGVEE92 pKa = 4.54 VARR95 pKa = 11.84 VAYY98 pKa = 10.29 DD99 pKa = 3.29 SAAQPNSVSTFTVADD114 pKa = 3.56 SGTYY118 pKa = 8.51 TVVVGADD125 pKa = 3.34 NSSTGSFEE133 pKa = 3.97 FRR135 pKa = 11.84 LVEE138 pKa = 4.2 LDD140 pKa = 3.71 GNNLTAIDD148 pKa = 4.87 ADD150 pKa = 3.97 TAGDD154 pKa = 4.1 FTASGEE160 pKa = 3.71 RR161 pKa = 11.84 DD162 pKa = 3.38 VFHH165 pKa = 7.52 FEE167 pKa = 3.61 GAAGRR172 pKa = 11.84 EE173 pKa = 3.91 VNLRR177 pKa = 11.84 IDD179 pKa = 3.31 AWGTAAQMSAASGRR193 pKa = 11.84 LVDD196 pKa = 3.92 NGGVEE201 pKa = 4.39 DD202 pKa = 5.51 GYY204 pKa = 11.49 DD205 pKa = 4.01 AIDD208 pKa = 3.74 EE209 pKa = 4.51 ALQHH213 pKa = 6.79 LRR215 pKa = 11.84 FRR217 pKa = 11.84 DD218 pKa = 3.37 NAATHH223 pKa = 6.95 FILITDD229 pKa = 4.03 EE230 pKa = 5.5 DD231 pKa = 4.6 RR232 pKa = 11.84 DD233 pKa = 3.94 NTSPNLTYY241 pKa = 11.14 NDD243 pKa = 3.86 IFNDD247 pKa = 3.78 LDD249 pKa = 3.74 TAGYY253 pKa = 7.18 TLHH256 pKa = 7.13 SIVNMQVDD264 pKa = 3.91 TTNQSEE270 pKa = 4.24 NALGIDD276 pKa = 3.95 GAGTSNSAYY285 pKa = 10.35 YY286 pKa = 10.96 ADD288 pKa = 3.98 GSGGYY293 pKa = 8.15 TSEE296 pKa = 4.24 TSGGVAFTRR305 pKa = 11.84 YY306 pKa = 6.83 STPVFDD312 pKa = 4.47 YY313 pKa = 10.92 NNNITTEE320 pKa = 3.73 ADD322 pKa = 3.88 YY323 pKa = 11.28 IDD325 pKa = 4.52 LAFDD329 pKa = 4.75 LEE331 pKa = 4.41 GTTWDD336 pKa = 3.62 LHH338 pKa = 4.87 QLRR341 pKa = 11.84 AGGLTGATAAQVLATEE357 pKa = 4.6 SFTAAFVDD365 pKa = 3.27 ILTNKK370 pKa = 7.8 ITADD374 pKa = 3.37 QMDD377 pKa = 5.04 LVSTDD382 pKa = 3.52 PNVTFEE388 pKa = 4.54 ILSEE392 pKa = 4.12 TLTEE396 pKa = 5.03 DD397 pKa = 5.04 DD398 pKa = 2.98 ISYY401 pKa = 10.27 DD402 pKa = 3.44 IRR404 pKa = 11.84 FTGDD408 pKa = 2.52 GTQYY412 pKa = 11.01 VFDD415 pKa = 4.16 LQFVRR420 pKa = 11.84 DD421 pKa = 3.78 QNGTDD426 pKa = 3.44 TVLGTIPVTLRR437 pKa = 11.84 THH439 pKa = 6.31 YY440 pKa = 10.91 VQLTEE445 pKa = 3.97 NSNFQTLYY453 pKa = 10.65 EE454 pKa = 4.48 DD455 pKa = 4.52 ALTIPADD462 pKa = 3.66 AEE464 pKa = 3.99 ALEE467 pKa = 4.93 LRR469 pKa = 11.84 FFDD472 pKa = 5.0 LQFDD476 pKa = 4.19 TADD479 pKa = 3.33 GAAINDD485 pKa = 3.64 AFEE488 pKa = 4.35 IALLDD493 pKa = 4.16 SNGNPLAGTISGGRR507 pKa = 11.84 DD508 pKa = 2.7 AFFNMTEE515 pKa = 4.31 GEE517 pKa = 4.26 DD518 pKa = 3.73 PLLGGGVVYY527 pKa = 10.38 DD528 pKa = 4.34 AEE530 pKa = 4.7 TGTVTLDD537 pKa = 3.06 ISHH540 pKa = 6.85 LPEE543 pKa = 4.74 GLAATLAVRR552 pKa = 11.84 LVNNDD557 pKa = 3.31 ADD559 pKa = 4.04 STSTVSVDD567 pKa = 2.93 VHH569 pKa = 5.3 PTILTTSPVANPSLTSPSGDD589 pKa = 2.4 GHH591 pKa = 6.93 APNTIDD597 pKa = 3.78 FDD599 pKa = 4.18 HH600 pKa = 6.5 MVDD603 pKa = 3.31 VTAAVSWDD611 pKa = 3.63 YY612 pKa = 11.32 QVTSFDD618 pKa = 3.85 EE619 pKa = 4.68 SSDD622 pKa = 3.56 TLFTGLTLTNDD633 pKa = 4.76 GISPIRR639 pKa = 11.84 GPLLVAVHH647 pKa = 6.98 HH648 pKa = 6.94 IDD650 pKa = 3.47 NPRR653 pKa = 11.84 VQATGYY659 pKa = 10.89 DD660 pKa = 3.38 GLTPNGYY667 pKa = 10.58 AYY669 pKa = 10.79 FDD671 pKa = 3.67 VTHH674 pKa = 7.01 LMEE677 pKa = 7.78 DD678 pKa = 3.66 GMLDD682 pKa = 3.32 PAAALSGLALRR693 pKa = 11.84 FHH695 pKa = 7.13 NPQQVQFNYY704 pKa = 10.11 EE705 pKa = 4.01 LQILADD711 pKa = 4.38 LNQPPEE717 pKa = 4.25 FVSDD721 pKa = 3.63 PVTEE725 pKa = 4.43 VVAGEE730 pKa = 4.24 SYY732 pKa = 10.55 EE733 pKa = 4.03 YY734 pKa = 10.85 DD735 pKa = 3.47 AAAVDD740 pKa = 4.0 PDD742 pKa = 4.03 GDD744 pKa = 3.76 AVTYY748 pKa = 10.56 SILSGPGTMAIDD760 pKa = 4.16 AEE762 pKa = 4.51 TGMITWSPTSGDD774 pKa = 3.3 VGGHH778 pKa = 4.2 VVIVQATDD786 pKa = 3.45 PLGSYY791 pKa = 10.5 DD792 pKa = 3.82 LQTYY796 pKa = 6.94 TLSVSEE802 pKa = 4.44 NVPNRR807 pKa = 11.84 PPLFTTIPIVDD818 pKa = 4.56 AYY820 pKa = 11.6 LHH822 pKa = 5.1 TTYY825 pKa = 10.3 FYY827 pKa = 11.15 DD828 pKa = 4.83 SDD830 pKa = 4.63 ASDD833 pKa = 4.28 PDD835 pKa = 4.58 GDD837 pKa = 4.36 DD838 pKa = 3.2 LTYY841 pKa = 11.35 SLVDD845 pKa = 4.16 GPQGMAIDD853 pKa = 5.01 ADD855 pKa = 3.87 TGAISWDD862 pKa = 3.55 VTDD865 pKa = 3.81 VSLVGTLVSVTLQVTEE881 pKa = 4.33 TGEE884 pKa = 4.01 SGAGHH889 pKa = 6.97 EE890 pKa = 4.41 ATQEE894 pKa = 3.77 YY895 pKa = 10.33 LIAVHH900 pKa = 5.94 EE901 pKa = 5.05 AIGNHH906 pKa = 6.15 DD907 pKa = 5.22 PIIISDD913 pKa = 3.78 PQLTFEE919 pKa = 5.2 LPGFSGNTATGDD931 pKa = 3.47 VTPTEE936 pKa = 4.48 LEE938 pKa = 3.99 LHH940 pKa = 6.43 LSAGEE945 pKa = 3.87 VSEE948 pKa = 4.74 QIVSLDD954 pKa = 3.37 YY955 pKa = 10.93 EE956 pKa = 4.26 YY957 pKa = 11.22 DD958 pKa = 3.74 GNFSADD964 pKa = 2.64 IVLIVDD970 pKa = 4.28 EE971 pKa = 4.72 SVSMTGGQAWLDD983 pKa = 4.26 EE984 pKa = 4.21 IAEE987 pKa = 4.19 ALNAEE992 pKa = 4.68 LEE994 pKa = 4.2 AAGYY998 pKa = 9.42 FEE1000 pKa = 4.82 NRR1002 pKa = 11.84 FAIVGFTADD1011 pKa = 3.5 SAVLSTVDD1019 pKa = 3.23 DD1020 pKa = 4.14 PFRR1023 pKa = 11.84 VSVYY1027 pKa = 10.65 GPDD1030 pKa = 3.54 DD1031 pKa = 4.15 ALVKK1035 pKa = 10.4 SVWVPEE1041 pKa = 4.09 RR1042 pKa = 11.84 EE1043 pKa = 4.32 LFSSALAGWEE1053 pKa = 4.14 LPSSGEE1059 pKa = 3.75 YY1060 pKa = 10.29 SVVVEE1065 pKa = 4.26 NTDD1068 pKa = 2.89 GSTATEE1074 pKa = 3.92 YY1075 pKa = 11.27 SFVLEE1080 pKa = 4.24 TVEE1083 pKa = 4.7 EE1084 pKa = 4.33 DD1085 pKa = 4.01 VSTQSLTLGTSYY1097 pKa = 10.99 SGTIEE1102 pKa = 4.01 TYY1104 pKa = 10.44 AAQDD1108 pKa = 3.87 DD1109 pKa = 4.63 YY1110 pKa = 11.34 TFTIAADD1117 pKa = 3.66 DD1118 pKa = 3.94 TLVLFDD1124 pKa = 5.13 ALANNDD1130 pKa = 3.52 QLQWSLTGPAGAVVSDD1146 pKa = 4.62 RR1147 pKa = 11.84 SLDD1150 pKa = 3.63 QPDD1153 pKa = 3.88 SVLSLDD1159 pKa = 3.68 SGSYY1163 pKa = 8.92 TLAIEE1168 pKa = 4.27 AVGFRR1173 pKa = 11.84 SEE1175 pKa = 5.13 DD1176 pKa = 3.39 YY1177 pKa = 10.77 QFRR1180 pKa = 11.84 LVDD1183 pKa = 4.32 LSAATSVTLGNSTSGTLDD1201 pKa = 3.31 PVGEE1205 pKa = 4.43 SVGYY1209 pKa = 8.19 TVTISTAARR1218 pKa = 11.84 YY1219 pKa = 9.9 DD1220 pKa = 3.7 FTAEE1224 pKa = 3.93 SWDD1227 pKa = 3.37 GSAAARR1233 pKa = 11.84 WRR1235 pKa = 11.84 LVDD1238 pKa = 3.57 DD1239 pKa = 4.0 TGTEE1243 pKa = 4.02 VFNALLSEE1251 pKa = 4.29 TQSLVSLAVGDD1262 pKa = 3.88 YY1263 pKa = 11.17 VLIIDD1268 pKa = 5.26 GDD1270 pKa = 3.8 VDD1272 pKa = 4.56 DD1273 pKa = 5.57 PASQPQYY1280 pKa = 10.8 QFTVEE1285 pKa = 4.01 SGTYY1289 pKa = 9.38 SLSGTAISLDD1299 pKa = 3.33 QTVSDD1304 pKa = 4.52 NLTSGQYY1311 pKa = 11.02 NFTLSADD1318 pKa = 3.26 ALVYY1322 pKa = 10.44 FDD1324 pKa = 3.62 VTEE1327 pKa = 4.73 EE1328 pKa = 3.93 ISGGTTQTMSWTLEE1342 pKa = 4.07 GADD1345 pKa = 3.57 GTFVSSRR1352 pKa = 11.84 WLEE1355 pKa = 3.65 EE1356 pKa = 4.33 SNAGGYY1362 pKa = 9.62 SGSDD1366 pKa = 3.38 PDD1368 pKa = 4.54 PLVFDD1373 pKa = 5.15 LSAGGYY1379 pKa = 10.06 NLTIDD1384 pKa = 3.55 NSSLNDD1390 pKa = 3.24 YY1391 pKa = 8.48 EE1392 pKa = 4.37 FRR1394 pKa = 11.84 VVDD1397 pKa = 3.15 IHH1399 pKa = 6.12 STLLSGVSSLTLDD1412 pKa = 3.51 TVVNGTLNSARR1423 pKa = 11.84 EE1424 pKa = 3.98 THH1426 pKa = 6.66 SYY1428 pKa = 9.38 TFSATVGDD1436 pKa = 4.21 VLAFDD1441 pKa = 5.15 ALTSAAQSSARR1452 pKa = 11.84 WRR1454 pKa = 11.84 LIGPDD1459 pKa = 3.63 GSVLSTNGLLTDD1471 pKa = 4.14 LPVYY1475 pKa = 10.8 VITQTGDD1482 pKa = 3.12 YY1483 pKa = 10.58 ALLIEE1488 pKa = 5.17 GGTDD1492 pKa = 3.28 DD1493 pKa = 4.78 SSTSRR1498 pKa = 11.84 TYY1500 pKa = 10.93 SFEE1503 pKa = 4.19 VQSLRR1508 pKa = 11.84 LATVGSLTDD1517 pKa = 3.34 GTLAADD1523 pKa = 3.46 EE1524 pKa = 4.12 THH1526 pKa = 7.37 RR1527 pKa = 11.84 YY1528 pKa = 9.83 GFTLDD1533 pKa = 4.09 EE1534 pKa = 4.61 DD1535 pKa = 3.81 ALLYY1539 pKa = 10.46 FDD1541 pKa = 5.66 ALTDD1545 pKa = 3.58 EE1546 pKa = 6.17 ADD1548 pKa = 4.19 FAWQLLDD1555 pKa = 3.7 SSGSTIVSQRR1565 pKa = 11.84 LFTASDD1571 pKa = 3.69 GDD1573 pKa = 4.11 DD1574 pKa = 3.44 VSDD1577 pKa = 4.14 ANAVLDD1583 pKa = 4.64 LSAGTYY1589 pKa = 10.11 VLDD1592 pKa = 3.76 VLSLNSATGDD1602 pKa = 3.28 YY1603 pKa = 11.0 DD1604 pKa = 3.89 LRR1606 pKa = 11.84 LVNLADD1612 pKa = 3.62 VAASDD1617 pKa = 4.17 FLGTTGDD1624 pKa = 3.85 ALSAGNSTDD1633 pKa = 2.8 VYY1635 pKa = 10.36 RR1636 pKa = 11.84 FQAIPGDD1643 pKa = 3.87 TLTIDD1648 pKa = 3.37 VSSYY1652 pKa = 10.71 SGPSGSQWRR1661 pKa = 11.84 VVNLDD1666 pKa = 3.17 GTVIDD1671 pKa = 4.57 SGLISASQDD1680 pKa = 3.38 TVSITAHH1687 pKa = 5.6 GEE1689 pKa = 3.91 YY1690 pKa = 10.6 YY1691 pKa = 10.98 LLIEE1695 pKa = 4.87 GDD1697 pKa = 3.59 ISDD1700 pKa = 4.03 TASSQSYY1707 pKa = 8.25 TLDD1710 pKa = 3.19 ATLSQGGTPTALTLGSTTSGTINSADD1736 pKa = 3.56 TFDD1739 pKa = 4.05 VYY1741 pKa = 11.08 SISGTAGQEE1750 pKa = 4.25 LFLDD1754 pKa = 4.06 WLSGATTDD1762 pKa = 3.3 MTLWLRR1768 pKa = 11.84 DD1769 pKa = 3.17 ADD1771 pKa = 4.33 GNLLLSEE1778 pKa = 4.37 SRR1780 pKa = 11.84 SEE1782 pKa = 3.9 EE1783 pKa = 3.5 SDD1785 pKa = 3.43 YY1786 pKa = 11.75 TKK1788 pKa = 10.89 FPLVTLATTGTYY1800 pKa = 9.42 TLLVGDD1806 pKa = 4.23 AAGGTGAYY1814 pKa = 9.53 SFRR1817 pKa = 11.84 VLDD1820 pKa = 4.63 KK1821 pKa = 10.11 ATQSQLVLDD1830 pKa = 5.15 DD1831 pKa = 3.87 EE1832 pKa = 4.8 TSSVFTATDD1841 pKa = 3.09 EE1842 pKa = 4.04 DD1843 pKa = 3.61 RR1844 pKa = 11.84 LYY1846 pKa = 10.86 RR1847 pKa = 11.84 FSGTAGQKK1855 pKa = 8.98 MVLRR1859 pKa = 11.84 LDD1861 pKa = 3.4 AWADD1865 pKa = 3.6 AEE1867 pKa = 5.24 DD1868 pKa = 4.05 VDD1870 pKa = 6.27 DD1871 pKa = 3.72 MTSRR1875 pKa = 11.84 LKK1877 pKa = 10.19 TEE1879 pKa = 4.31 GVIEE1883 pKa = 4.62 DD1884 pKa = 4.41 GYY1886 pKa = 10.85 EE1887 pKa = 4.36 AIHH1890 pKa = 6.1 TALNGLNFRR1899 pKa = 11.84 DD1900 pKa = 3.75 KK1901 pKa = 11.08 VSRR1904 pKa = 11.84 QLVLITDD1911 pKa = 4.12 EE1912 pKa = 5.98 DD1913 pKa = 5.4 RR1914 pKa = 11.84 DD1915 pKa = 4.1 TTDD1918 pKa = 5.02 ANLTYY1923 pKa = 10.09 NTILDD1928 pKa = 4.05 EE1929 pKa = 4.72 LDD1931 pKa = 3.2 EE1932 pKa = 6.18 HH1933 pKa = 7.08 DD1934 pKa = 5.24 ASLHH1938 pKa = 5.72 SLLDD1942 pKa = 3.72 VILNEE1947 pKa = 4.45 VPGSGPYY1954 pKa = 10.87 ALGTDD1959 pKa = 3.77 GDD1961 pKa = 4.21 TAADD1965 pKa = 3.24 NMYY1968 pKa = 10.09 WIGTGGDD1975 pKa = 3.58 YY1976 pKa = 10.79 TVTSGTAPNLSTSWAIEE1993 pKa = 3.85 TEE1995 pKa = 4.4 YY1996 pKa = 10.85 IDD1998 pKa = 4.37 LTFDD2002 pKa = 4.03 ADD2004 pKa = 3.69 GTVWDD2009 pKa = 4.68 GLTLEE2014 pKa = 4.45 WGVPDD2019 pKa = 4.01 EE2020 pKa = 4.54 VNSFIAALADD2030 pKa = 3.94 VIRR2033 pKa = 11.84 GDD2035 pKa = 3.38 VSEE2038 pKa = 5.27 DD2039 pKa = 3.39 LDD2041 pKa = 3.98 VTLTASDD2048 pKa = 4.41 PNVQFEE2054 pKa = 4.83 ILTKK2058 pKa = 9.78 TYY2060 pKa = 10.77 NGTSFDD2066 pKa = 3.53 FDD2068 pKa = 3.51 VRR2070 pKa = 11.84 ITGDD2074 pKa = 3.8 GNPHH2078 pKa = 6.67 AFDD2081 pKa = 3.55 LQFVRR2086 pKa = 11.84 EE2087 pKa = 4.18 NNPGVVVGTIPTEE2100 pKa = 3.23 IWVDD2104 pKa = 3.06 YY2105 pKa = 10.09 FYY2107 pKa = 11.3 DD2108 pKa = 3.53 VDD2110 pKa = 6.44 AIDD2113 pKa = 5.02 PDD2115 pKa = 4.71 DD2116 pKa = 6.12 DD2117 pKa = 3.91 EE2118 pKa = 4.47 LTYY2121 pKa = 10.68 TLVGEE2126 pKa = 4.32 THH2128 pKa = 6.93 GATFDD2133 pKa = 3.76 NEE2135 pKa = 4.26 TGLLRR2140 pKa = 11.84 WAPTVTGEE2148 pKa = 4.33 YY2149 pKa = 10.25 EE2150 pKa = 4.0 FTALVEE2156 pKa = 4.5 DD2157 pKa = 4.34 GRR2159 pKa = 11.84 GGSDD2163 pKa = 3.07 LQTWTVTVGDD2173 pKa = 4.03 TTSGNTAPVLASAGPFTIQSGRR2195 pKa = 11.84 PFEE2198 pKa = 4.37 FMAVASDD2205 pKa = 3.59 VDD2207 pKa = 4.31 GDD2209 pKa = 3.94 ALQYY2213 pKa = 10.62 QILDD2217 pKa = 3.48 IPGSANAKK2225 pKa = 7.62 PAGMTIDD2232 pKa = 4.14 PLTGVVSWTPDD2243 pKa = 2.93 VLQAGEE2249 pKa = 4.02 YY2250 pKa = 10.5 DD2251 pKa = 3.51 IVIRR2255 pKa = 11.84 AADD2258 pKa = 3.59 GRR2260 pKa = 11.84 GGIDD2264 pKa = 3.97 SITVSITVVVPTIIEE2279 pKa = 4.3 TLDD2282 pKa = 3.28 NAHH2285 pKa = 6.65 PVIVSTPVTSAMLGIQYY2302 pKa = 9.67 TYY2304 pKa = 11.21 DD2305 pKa = 3.54 VQATDD2310 pKa = 4.31 ADD2312 pKa = 4.86 LDD2314 pKa = 3.92 PLLYY2318 pKa = 10.37 EE2319 pKa = 5.04 LALAPDD2325 pKa = 4.38 GMAIDD2330 pKa = 4.66 PVTGLISWVPSFSDD2344 pKa = 4.54 LDD2346 pKa = 3.6 DD2347 pKa = 4.9 TIAGQHH2353 pKa = 5.37 HH2354 pKa = 5.88 VAVRR2358 pKa = 11.84 VTDD2361 pKa = 3.7 GRR2363 pKa = 11.84 GGIALQKK2370 pKa = 10.54 FIVDD2374 pKa = 4.28 LQAGLPIITSSPNLSATVGTEE2395 pKa = 3.24 WTYY2398 pKa = 11.62 QLTASDD2404 pKa = 4.55 PDD2406 pKa = 3.71 LPNDD2410 pKa = 3.31 VLEE2413 pKa = 4.43 YY2414 pKa = 10.31 EE2415 pKa = 4.42 LTYY2418 pKa = 11.09 GPDD2421 pKa = 3.31 GATIGASDD2429 pKa = 4.99 GLLSWTPTATGTNLFVVTVYY2449 pKa = 11.18 DD2450 pKa = 4.01 LAGHH2454 pKa = 7.31 GYY2456 pKa = 8.5 QQMFLLPVIDD2466 pKa = 4.24 TGSNTAPTILSTPRR2480 pKa = 11.84 THH2482 pKa = 7.59 TIADD2486 pKa = 3.44 QTFLTRR2492 pKa = 11.84 ITASDD2497 pKa = 3.87 PEE2499 pKa = 4.82 GEE2501 pKa = 4.41 VITYY2505 pKa = 10.29 SLIDD2509 pKa = 4.26 GPSTMTIDD2517 pKa = 3.68 GATGMVQWLPGLEE2530 pKa = 4.08 DD2531 pKa = 3.63 VNAVSAPHH2539 pKa = 6.24 QYY2541 pKa = 8.51 TVRR2544 pKa = 11.84 ATDD2547 pKa = 3.12 ARR2549 pKa = 11.84 GLYY2552 pKa = 10.21 DD2553 pKa = 3.65 EE2554 pKa = 4.56 QTFDD2558 pKa = 3.57 LHH2560 pKa = 7.31 VVAPLVDD2567 pKa = 4.56 DD2568 pKa = 5.67 DD2569 pKa = 4.35 GTSVNHH2575 pKa = 6.13 SPEE2578 pKa = 3.98 ITSTPDD2584 pKa = 2.95 KK2585 pKa = 10.84 VWIVDD2590 pKa = 3.33 RR2591 pKa = 11.84 LYY2593 pKa = 10.62 RR2594 pKa = 11.84 YY2595 pKa = 8.95 QATATDD2601 pKa = 3.91 ADD2603 pKa = 4.11 SDD2605 pKa = 3.95 QLLWSLDD2612 pKa = 3.34 SDD2614 pKa = 4.27 VANMEE2619 pKa = 4.11 IDD2621 pKa = 3.6 PATGEE2626 pKa = 4.58 LLWLPDD2632 pKa = 3.38 ASQIGNTEE2640 pKa = 3.57 VTIRR2644 pKa = 11.84 VIDD2647 pKa = 4.03 PFGGLATQSFVISVSDD2663 pKa = 3.79 SNHH2666 pKa = 5.92 PPEE2669 pKa = 4.64 ITSDD2673 pKa = 3.68 PPQSGRR2679 pKa = 11.84 SGEE2682 pKa = 4.06 AYY2684 pKa = 8.99 TYY2686 pKa = 10.7 NVEE2689 pKa = 4.11 ATDD2692 pKa = 4.07 PDD2694 pKa = 4.42 EE2695 pKa = 4.59 DD2696 pKa = 4.24 TLVYY2700 pKa = 10.89 ALDD2703 pKa = 4.04 NPPTGMSIDD2712 pKa = 3.82 SDD2714 pKa = 3.69 TGVISWTPSSEE2725 pKa = 3.83 GSHH2728 pKa = 5.86 SVTVTATDD2736 pKa = 3.59 PSGWYY2741 pKa = 9.9 AYY2743 pKa = 7.45 QTYY2746 pKa = 10.73 DD2747 pKa = 3.28 LTIYY2751 pKa = 10.74 APASNLPPQINSNPEE2766 pKa = 3.48 QTGMVGTAYY2775 pKa = 10.09 SYY2777 pKa = 10.9 QVIAFDD2783 pKa = 4.32 PDD2785 pKa = 3.57 EE2786 pKa = 6.07 DD2787 pKa = 3.95 EE2788 pKa = 5.55 LDD2790 pKa = 3.73 YY2791 pKa = 11.61 SLGGTPPIGMSIDD2804 pKa = 4.04 PDD2806 pKa = 3.72 TGLLTWTPAAGQTGEE2821 pKa = 4.64 HH2822 pKa = 5.8 SVQVIVSDD2830 pKa = 4.06 GTDD2833 pKa = 3.06 SDD2835 pKa = 4.1 DD2836 pKa = 3.43 QTFEE2840 pKa = 3.64 ISVRR2844 pKa = 11.84 DD2845 pKa = 3.46 NAAPVLTAIPDD2856 pKa = 3.74 GTLTAGQLYY2865 pKa = 9.75 EE2866 pKa = 4.46 FDD2868 pKa = 4.35 VSATDD2873 pKa = 3.64 ADD2875 pKa = 4.4 GDD2877 pKa = 4.06 ALTYY2881 pKa = 10.83 SLLGAPSNMTIDD2893 pKa = 3.57 AGTGRR2898 pKa = 11.84 IRR2900 pKa = 11.84 WQTTLDD2906 pKa = 4.58 DD2907 pKa = 4.6 INLHH2911 pKa = 5.84 SDD2913 pKa = 2.7 ITVAVTDD2920 pKa = 4.16 PFGLYY2925 pKa = 8.07 DD2926 pKa = 3.72 TLMFDD2931 pKa = 5.05 LDD2933 pKa = 4.46 VVPPAADD2940 pKa = 3.89 DD2941 pKa = 4.53 GGDD2944 pKa = 3.75 GNSGDD2949 pKa = 4.06 GGGPSINSYY2958 pKa = 10.57 PYY2960 pKa = 8.32 ITSTPSYY2967 pKa = 9.13 TAVVDD2972 pKa = 4.04 NLYY2975 pKa = 10.49 LYY2977 pKa = 10.4 QATATDD2983 pKa = 4.41 PDD2985 pKa = 4.28 HH2986 pKa = 7.88 DD2987 pKa = 4.35 PLEE2990 pKa = 4.29 WLLDD2994 pKa = 3.6 SSVRR2998 pKa = 11.84 NMQIDD3003 pKa = 4.04 RR3004 pKa = 11.84 DD3005 pKa = 3.94 TGLVSWSPDD3014 pKa = 2.87 EE3015 pKa = 5.03 DD3016 pKa = 3.58 QIGDD3020 pKa = 3.7 VEE3022 pKa = 4.76 VKK3024 pKa = 10.26 VRR3026 pKa = 11.84 ARR3028 pKa = 11.84 DD3029 pKa = 3.48 PFGASSQQTFLISVVGTNQTPLFTTDD3055 pKa = 4.83 PITSGRR3061 pKa = 11.84 VGDD3064 pKa = 4.44 DD3065 pKa = 3.14 YY3066 pKa = 11.82 SYY3068 pKa = 11.4 AANAIDD3074 pKa = 4.83 PDD3076 pKa = 4.5 GDD3078 pKa = 3.9 PLTYY3082 pKa = 11.07 ALLDD3086 pKa = 4.04 EE3087 pKa = 4.64 PTGMTIDD3094 pKa = 5.66 PITGVISWTPGATGTSEE3111 pKa = 4.62 VIVTATDD3118 pKa = 3.76 PSGLAAEE3125 pKa = 4.25 QTYY3128 pKa = 10.75 QLVITAAATNGRR3140 pKa = 11.84 PEE3142 pKa = 4.2 ITSTPDD3148 pKa = 3.08 WIALVGSEE3156 pKa = 3.89 YY3157 pKa = 10.08 TYY3159 pKa = 11.16 QVTATDD3165 pKa = 4.34 PDD3167 pKa = 4.92 DD3168 pKa = 4.14 DD3169 pKa = 3.96 TLEE3172 pKa = 4.14 YY3173 pKa = 10.75 SLAGTPPNGMSIDD3186 pKa = 4.28 PDD3188 pKa = 3.66 TGLLTWTPNGGQIGTHH3204 pKa = 4.87 SLEE3207 pKa = 4.63 VIASDD3212 pKa = 4.12 GSLGDD3217 pKa = 3.49 VQSFDD3222 pKa = 4.28 LRR3224 pKa = 11.84 VRR3226 pKa = 11.84 DD3227 pKa = 3.73 NAAPTIAPIPDD3238 pKa = 3.48 LTRR3241 pKa = 11.84 AAGEE3245 pKa = 4.33 TYY3247 pKa = 9.76 QFRR3250 pKa = 11.84 AYY3252 pKa = 10.47 GSDD3255 pKa = 3.31 TDD3257 pKa = 4.96 GDD3259 pKa = 4.06 ALTYY3263 pKa = 10.0 EE3264 pKa = 4.77 LSGEE3268 pKa = 4.11 PAGMTINEE3276 pKa = 4.07 FTGNIEE3282 pKa = 4.03 WLTTIDD3288 pKa = 5.84 DD3289 pKa = 3.58 IGTYY3293 pKa = 10.84 ADD3295 pKa = 3.16 ITVTVTDD3302 pKa = 3.69 QYY3304 pKa = 11.75 GLSASEE3310 pKa = 4.2 TFDD3313 pKa = 4.47 LEE3315 pKa = 4.51 IIAPPTSGGGSNQNGGPHH3333 pKa = 5.72 ITSTAGSLAIVGEE3346 pKa = 4.28 QYY3348 pKa = 10.6 TYY3350 pKa = 11.07 QATATDD3356 pKa = 4.17 PDD3358 pKa = 4.61 GDD3360 pKa = 3.9 TLLWSLEE3367 pKa = 3.85 TDD3369 pKa = 3.79 VANMEE3374 pKa = 4.42 INPTTGEE3381 pKa = 4.25 LLWTPDD3387 pKa = 2.95 ASQIGTVSATILVSDD3402 pKa = 3.98 PHH3404 pKa = 6.44 GAWSLQEE3411 pKa = 3.45 IHH3413 pKa = 6.63 VAVVDD3418 pKa = 4.01 NNLPPQFTSTPPASGHH3434 pKa = 5.53 SDD3436 pKa = 3.05 EE3437 pKa = 6.07 DD3438 pKa = 3.33 YY3439 pKa = 10.42 TYY3441 pKa = 11.43 NVTATDD3447 pKa = 4.04 PEE3449 pKa = 4.66 EE3450 pKa = 4.04 EE3451 pKa = 4.31 TLTFSLSDD3459 pKa = 3.89 GPSGMTIDD3467 pKa = 4.57 PNTGVLTWTPTGEE3480 pKa = 4.11 GTFDD3484 pKa = 3.59 VVVTVADD3491 pKa = 3.61 PAGYY3495 pKa = 9.95 AVHH3498 pKa = 6.6 QDD3500 pKa = 3.01 YY3501 pKa = 11.18 DD3502 pKa = 3.77 LTIFAPAANLPPQITSTAVLTGMVGTAYY3530 pKa = 10.24 SYY3532 pKa = 11.12 QVTATDD3538 pKa = 4.35 PEE3540 pKa = 6.03 DD3541 pKa = 3.66 DD3542 pKa = 3.71 TLEE3545 pKa = 4.19 FSLGGTPPIGMSIDD3559 pKa = 4.0 PDD3561 pKa = 3.65 TGLLSWTPTSGQTGLNTFEE3580 pKa = 5.13 VIVSDD3585 pKa = 5.27 GNDD3588 pKa = 3.08 TDD3590 pKa = 3.81 TQYY3593 pKa = 11.81 LGISIRR3599 pKa = 11.84 DD3600 pKa = 3.51 NTAPTITTIPDD3611 pKa = 3.26 ATVTAGNGYY3620 pKa = 10.18 QYY3622 pKa = 11.23 DD3623 pKa = 3.97 VSAADD3628 pKa = 3.71 ADD3630 pKa = 4.34 GDD3632 pKa = 4.03 ALTYY3636 pKa = 10.46 SISGEE3641 pKa = 4.1 PTGMTIDD3648 pKa = 4.12 ADD3650 pKa = 3.67 TGRR3653 pKa = 11.84 IEE3655 pKa = 3.94 WQTAIGDD3662 pKa = 3.81 IGAHH3666 pKa = 5.84 ANITVTVTDD3675 pKa = 3.75 EE3676 pKa = 4.15 FGLYY3680 pKa = 8.51 NTDD3683 pKa = 3.36 TFDD3686 pKa = 3.4 VTVAADD3692 pKa = 3.66 DD3693 pKa = 3.97 TPPVVDD3699 pKa = 3.33 VTYY3702 pKa = 11.16 SHH3704 pKa = 7.71 DD3705 pKa = 3.79 PAQLGTPLSIYY3716 pKa = 9.62 IQAVDD3721 pKa = 3.85 DD3722 pKa = 4.24 VGVDD3726 pKa = 4.63 EE3727 pKa = 4.86 ITLDD3731 pKa = 3.45 INGVDD3736 pKa = 3.66 VAVDD3740 pKa = 3.48 ANGNAVYY3747 pKa = 10.35 DD3748 pKa = 4.24 VVSLDD3753 pKa = 3.71 PLVVTVTATDD3763 pKa = 3.49 TVDD3766 pKa = 3.11 NEE3768 pKa = 4.76 TIVVDD3773 pKa = 3.9 NLTVIDD3779 pKa = 4.91 PGDD3782 pKa = 3.46 VTAPEE3787 pKa = 4.0 VAITSITGGEE3797 pKa = 4.41 EE3798 pKa = 3.67 LTEE3801 pKa = 4.17 PTDD3804 pKa = 3.53 ILGTVDD3810 pKa = 5.75 DD3811 pKa = 5.34 LDD3813 pKa = 5.0 DD3814 pKa = 4.23 NLVYY3818 pKa = 8.29 WTLIATAIDD3827 pKa = 3.38 SGRR3830 pKa = 11.84 EE3831 pKa = 3.65 FTLATGGNEE3840 pKa = 3.42 ITTPALLGNFDD3851 pKa = 3.48 TTAIANGLYY3860 pKa = 10.35 RR3861 pKa = 11.84 LTLTAVDD3868 pKa = 3.89 AGGNTATDD3876 pKa = 3.4 NKK3878 pKa = 10.61 LVTVEE3883 pKa = 4.47 GNLKK3887 pKa = 10.42 LGAFSISFLDD3897 pKa = 3.65 MQIPAPGFPITITRR3911 pKa = 11.84 SYY3913 pKa = 9.31 STLDD3917 pKa = 3.16 AGEE3920 pKa = 4.25 QGDD3923 pKa = 4.8 FGYY3926 pKa = 10.86 GWSLDD3931 pKa = 3.51 IANTSVNVEE3940 pKa = 4.12 HH3941 pKa = 7.4 ADD3943 pKa = 3.66 SGLSNHH3949 pKa = 6.59 GPYY3952 pKa = 10.0 TPFKK3956 pKa = 10.36 NGDD3959 pKa = 3.42 RR3960 pKa = 11.84 VVITLPDD3967 pKa = 3.41 GTTEE3971 pKa = 4.03 GFTFYY3976 pKa = 11.2 AEE3978 pKa = 4.06 PAFSYY3983 pKa = 10.53 GIIAYY3988 pKa = 9.31 DD3989 pKa = 3.57 YY3990 pKa = 10.35 KK3991 pKa = 11.04 PKK3993 pKa = 10.46 FVADD3997 pKa = 3.78 AGVDD4001 pKa = 3.45 SEE4003 pKa = 5.5 LFVNEE4008 pKa = 4.4 ADD4010 pKa = 3.79 VHH4012 pKa = 5.6 LQQAGDD4018 pKa = 3.35 EE4019 pKa = 4.5 WVDD4022 pKa = 3.3 HH4023 pKa = 6.3 SKK4025 pKa = 10.76 QGLAYY4030 pKa = 10.42 NPALEE4035 pKa = 4.43 EE4036 pKa = 3.88 FGNAYY4041 pKa = 9.23 VLRR4044 pKa = 11.84 LRR4046 pKa = 11.84 TGVEE4050 pKa = 3.61 LTINATSGEE4059 pKa = 4.22 LASIHH4064 pKa = 6.77 DD4065 pKa = 4.19 RR4066 pKa = 11.84 YY4067 pKa = 11.36 GNALYY4072 pKa = 8.28 FTGDD4076 pKa = 3.73 GIEE4079 pKa = 4.16 SSNGRR4084 pKa = 11.84 GVDD4087 pKa = 4.18 FEE4089 pKa = 5.48 HH4090 pKa = 7.88 DD4091 pKa = 3.59 YY4092 pKa = 11.75 ADD4094 pKa = 5.55 RR4095 pKa = 11.84 ITKK4098 pKa = 8.41 ITDD4101 pKa = 3.08 HH4102 pKa = 6.46 VGNFLLYY4109 pKa = 10.45 DD4110 pKa = 3.81 YY4111 pKa = 11.65 DD4112 pKa = 4.32 EE4113 pKa = 4.84 DD4114 pKa = 4.01 TGNLIAVTDD4123 pKa = 3.82 RR4124 pKa = 11.84 TGATTQFTYY4133 pKa = 11.2 LDD4135 pKa = 4.11 GPNDD4139 pKa = 3.69 PEE4141 pKa = 5.8 HH4142 pKa = 6.92 YY4143 pKa = 9.79 LDD4145 pKa = 5.8 EE4146 pKa = 6.82 IIDD4149 pKa = 3.83 PLGRR4153 pKa = 11.84 SAATTTYY4160 pKa = 10.78 DD4161 pKa = 2.94 EE4162 pKa = 4.55 RR4163 pKa = 11.84 GRR4165 pKa = 11.84 VEE4167 pKa = 4.54 AVTDD4171 pKa = 3.9 ADD4173 pKa = 4.05 GQTISYY4179 pKa = 9.11 TYY4181 pKa = 11.02 DD4182 pKa = 3.15 SGNKK4186 pKa = 7.51 TQTITDD4192 pKa = 3.63 QLNYY4196 pKa = 9.67 TSTVTFDD4203 pKa = 2.99 ARR4205 pKa = 11.84 GNIIRR4210 pKa = 11.84 EE4211 pKa = 4.01 EE4212 pKa = 4.32 DD4213 pKa = 3.59 PLGGITKK4220 pKa = 9.02 RR4221 pKa = 11.84 TFDD4224 pKa = 5.24 DD4225 pKa = 4.67 DD4226 pKa = 5.71 DD4227 pKa = 4.38 NLLTEE4232 pKa = 4.16 IMVIGLEE4239 pKa = 4.17 DD4240 pKa = 3.68 TVSEE4244 pKa = 4.3 EE4245 pKa = 4.4 TNDD4248 pKa = 3.77 LLTTYY4253 pKa = 9.97 TYY4255 pKa = 8.36 DD4256 pKa = 3.19 TNGFVSSVITPDD4268 pKa = 3.45 GLEE4271 pKa = 3.96 TTYY4274 pKa = 11.17 VNHH4277 pKa = 6.0 EE4278 pKa = 4.36 TGTPLIVTDD4287 pKa = 3.82 PFGNTTEE4294 pKa = 4.44 TVVDD4298 pKa = 4.26 EE4299 pKa = 4.47 YY4300 pKa = 11.64 GRR4302 pKa = 11.84 PLYY4305 pKa = 9.92 LTDD4308 pKa = 3.69 ALEE4311 pKa = 4.25 NRR4313 pKa = 11.84 TQLIYY4318 pKa = 11.19 YY4319 pKa = 9.68 GVGTNAADD4327 pKa = 3.59 NGQVRR4332 pKa = 11.84 YY4333 pKa = 9.71 IVTPWKK4339 pKa = 9.3 TDD4341 pKa = 3.57 DD4342 pKa = 3.5 YY4343 pKa = 11.14 HH4344 pKa = 8.0 GPSDD4348 pKa = 3.54 NTAVSFEE4355 pKa = 3.98 YY4356 pKa = 10.67 DD4357 pKa = 3.21 EE4358 pKa = 4.63 EE4359 pKa = 6.03 GNLISTTNWDD4369 pKa = 3.35 QWSTPANAGDD4379 pKa = 3.56 GNYY4382 pKa = 7.61 RR4383 pKa = 11.84 TNRR4386 pKa = 11.84 FTYY4389 pKa = 10.48 DD4390 pKa = 3.34 DD4391 pKa = 4.1 YY4392 pKa = 11.92 GNQIGTSRR4400 pKa = 11.84 LWQDD4404 pKa = 3.29 PNDD4407 pKa = 4.0 AEE4409 pKa = 4.36 NTVTLTTSSSYY4420 pKa = 11.45 DD4421 pKa = 3.38 DD4422 pKa = 3.66 NNRR4425 pKa = 11.84 LISHH4429 pKa = 7.17 TDD4431 pKa = 3.26 AQGHH4435 pKa = 4.92 TTTTTYY4441 pKa = 11.21 DD4442 pKa = 3.03 VAGQVLSTTRR4452 pKa = 11.84 IIGEE4456 pKa = 4.35 DD4457 pKa = 3.52 DD4458 pKa = 3.41 STSAEE4463 pKa = 4.16 NDD4465 pKa = 3.57 DD4466 pKa = 3.73 QTTTMLYY4473 pKa = 10.42 DD4474 pKa = 3.38 PRR4476 pKa = 11.84 GQNIQTTYY4484 pKa = 11.29 SDD4486 pKa = 3.46 GTITRR4491 pKa = 11.84 TVYY4494 pKa = 9.98 DD4495 pKa = 3.54 AKK4497 pKa = 10.94 GRR4499 pKa = 11.84 AVYY4502 pKa = 7.93 TTDD4505 pKa = 2.63 RR4506 pKa = 11.84 HH4507 pKa = 6.37 LPADD4511 pKa = 4.13 DD4512 pKa = 3.88 TQGTYY4517 pKa = 10.77 YY4518 pKa = 10.03 IYY4520 pKa = 10.64 DD4521 pKa = 3.59 ALDD4524 pKa = 3.15 RR4525 pKa = 11.84 VVEE4528 pKa = 4.37 SKK4530 pKa = 10.65 RR4531 pKa = 11.84 YY4532 pKa = 9.1 DD4533 pKa = 3.43 GMVIDD4538 pKa = 4.74 VDD4540 pKa = 4.36 TVSGDD4545 pKa = 3.24 STTSLNTLGTLLYY4558 pKa = 10.16 SSSSTYY4564 pKa = 11.33 DD4565 pKa = 3.34 NVSGWLLSTTGSTGVVTTYY4584 pKa = 10.55 EE4585 pKa = 3.94 YY4586 pKa = 11.29 DD4587 pKa = 3.3 DD4588 pKa = 4.02 FGRR4591 pKa = 11.84 TKK4593 pKa = 10.67 KK4594 pKa = 8.94 ITEE4597 pKa = 4.2 TAGSEE4602 pKa = 4.26 SRR4604 pKa = 11.84 STEE4607 pKa = 3.49 YY4608 pKa = 10.52 TYY4610 pKa = 11.62 DD4611 pKa = 3.43 DD4612 pKa = 4.52 FGRR4615 pKa = 11.84 QTAVKK4620 pKa = 9.61 DD4621 pKa = 3.3 ALGRR4625 pKa = 11.84 YY4626 pKa = 6.51 TLTHH4630 pKa = 5.85 YY4631 pKa = 10.88 DD4632 pKa = 3.1 EE4633 pKa = 5.08 YY4634 pKa = 11.42 GRR4636 pKa = 11.84 SDD4638 pKa = 3.3 RR4639 pKa = 11.84 TTFDD4643 pKa = 3.51 DD4644 pKa = 4.04 GTFVEE4649 pKa = 4.6 TTYY4652 pKa = 11.58 DD4653 pKa = 2.94 KK4654 pKa = 10.78 FGRR4657 pKa = 11.84 VVGEE4661 pKa = 4.02 TDD4663 pKa = 3.97 QMGQTKK4669 pKa = 9.26 TYY4671 pKa = 10.11 EE4672 pKa = 3.83 YY4673 pKa = 10.57 DD4674 pKa = 3.55 PQHH4677 pKa = 6.1 GRR4679 pKa = 11.84 LTAVVLPAVINPATDD4694 pKa = 3.13 QLEE4697 pKa = 4.29 NPRR4700 pKa = 11.84 YY4701 pKa = 8.85 EE4702 pKa = 4.03 YY4703 pKa = 10.65 EE4704 pKa = 3.55 YY4705 pKa = 10.55 DD4706 pKa = 3.53 YY4707 pKa = 11.53 YY4708 pKa = 11.68 GNQTVIRR4715 pKa = 11.84 DD4716 pKa = 3.61 PLGRR4720 pKa = 11.84 EE4721 pKa = 3.67 TTFTYY4726 pKa = 11.11 NEE4728 pKa = 4.2 FGQQVTRR4735 pKa = 11.84 EE4736 pKa = 4.12 LPLGQTEE4743 pKa = 4.62 YY4744 pKa = 9.42 FTYY4747 pKa = 10.52 DD4748 pKa = 2.63 EE4749 pKa = 4.21 YY4750 pKa = 11.71 GRR4752 pKa = 11.84 RR4753 pKa = 11.84 EE4754 pKa = 3.87 LHH4756 pKa = 6.24 TSFEE4760 pKa = 4.68 GNLTEE4765 pKa = 5.56 SVYY4768 pKa = 11.37 SDD4770 pKa = 4.55 LGFLDD4775 pKa = 3.48 EE4776 pKa = 4.64 LRR4778 pKa = 11.84 YY4779 pKa = 10.12 YY4780 pKa = 10.64 EE4781 pKa = 5.13 PGDD4784 pKa = 4.32 DD4785 pKa = 4.51 PSIDD4789 pKa = 3.62 PADD4792 pKa = 3.54 EE4793 pKa = 4.18 VVAFVYY4799 pKa = 10.59 DD4800 pKa = 3.55 DD4801 pKa = 4.07 EE4802 pKa = 4.71 FRR4804 pKa = 11.84 QTSVTDD4810 pKa = 3.62 GRR4812 pKa = 11.84 GEE4814 pKa = 4.15 TEE4816 pKa = 3.34 FDD4818 pKa = 3.19 YY4819 pKa = 11.66 DD4820 pKa = 3.56 EE4821 pKa = 5.08 FDD4823 pKa = 4.26 RR4824 pKa = 11.84 LSQVDD4829 pKa = 3.96 APEE4832 pKa = 4.16 GTLNYY4837 pKa = 10.22 EE4838 pKa = 3.72 YY4839 pKa = 10.82 DD4840 pKa = 3.27 ALGRR4844 pKa = 11.84 KK4845 pKa = 6.32 TRR4847 pKa = 11.84 AYY4849 pKa = 9.24 IGSPADD4855 pKa = 3.49 PTHH4858 pKa = 6.5 DD4859 pKa = 3.08 WEE4861 pKa = 4.44 YY4862 pKa = 10.84 TYY4864 pKa = 10.99 DD4865 pKa = 3.01 AWGRR4869 pKa = 11.84 LLDD4872 pKa = 3.65 VKK4874 pKa = 10.18 TIEE4877 pKa = 4.7 RR4878 pKa = 11.84 NDD4880 pKa = 3.66 EE4881 pKa = 4.08 VLSTPEE4887 pKa = 3.82 VTTYY4891 pKa = 10.97 GYY4893 pKa = 8.69 DD4894 pKa = 3.39 TIGNLQRR4901 pKa = 11.84 IDD4903 pKa = 3.86 HH4904 pKa = 6.79 ANGMITFYY4912 pKa = 10.79 EE4913 pKa = 4.2 YY4914 pKa = 9.28 DD4915 pKa = 3.36 TLNRR4919 pKa = 11.84 LDD4921 pKa = 3.34 VLTYY4925 pKa = 10.42 YY4926 pKa = 10.83 DD4927 pKa = 4.7 ADD4929 pKa = 3.83 GTPLDD4934 pKa = 4.42 LSDD4937 pKa = 3.5 NAKK4940 pKa = 10.45 LYY4942 pKa = 10.63 EE4943 pKa = 3.88 FDD4945 pKa = 3.57 YY4946 pKa = 10.78 TVRR4949 pKa = 11.84 ADD4951 pKa = 3.4 GRR4953 pKa = 11.84 RR4954 pKa = 11.84 AALTEE4959 pKa = 4.41 TYY4961 pKa = 9.6 WDD4963 pKa = 3.37 STTPYY4968 pKa = 9.3 VTTTNWTYY4976 pKa = 11.41 DD4977 pKa = 3.17 ALNRR4981 pKa = 11.84 LVEE4984 pKa = 4.21 EE4985 pKa = 5.3 EE4986 pKa = 4.42 YY4987 pKa = 10.93 DD4988 pKa = 4.02 SHH4990 pKa = 9.21 DD4991 pKa = 4.99 DD4992 pKa = 3.87 DD4993 pKa = 6.41 LDD4995 pKa = 3.75 YY4996 pKa = 10.65 ATTFEE5001 pKa = 4.26 YY5002 pKa = 11.16 DD5003 pKa = 3.22 LVGNRR5008 pKa = 11.84 LSKK5011 pKa = 11.09 VIDD5014 pKa = 4.45 GIIDD5018 pKa = 3.27 KK5019 pKa = 9.53 TLTYY5023 pKa = 10.26 EE5024 pKa = 3.96 YY5025 pKa = 10.69 DD5026 pKa = 3.68 DD5027 pKa = 4.11 NDD5029 pKa = 4.32 RR5030 pKa = 11.84 LLTSWKK5036 pKa = 10.27 IIGEE5040 pKa = 3.89 VDD5042 pKa = 3.37 DD5043 pKa = 4.98 AVNLEE5048 pKa = 4.44 TNDD5051 pKa = 3.13 TTTTYY5056 pKa = 10.49 GYY5058 pKa = 10.73 SGTLQTSKK5066 pKa = 9.38 TVKK5069 pKa = 8.72 EE5070 pKa = 4.55 TYY5072 pKa = 8.78 STQTLSVVDD5081 pKa = 3.67 YY5082 pKa = 10.2 EE5083 pKa = 4.57 YY5084 pKa = 11.46 NLQGRR5089 pKa = 11.84 MSKK5092 pKa = 10.46 VVIDD5096 pKa = 4.36 TYY5098 pKa = 11.49 DD5099 pKa = 3.42 SNGDD5103 pKa = 3.49 VVKK5106 pKa = 10.83 RR5107 pKa = 11.84 EE5108 pKa = 4.0 TTEE5111 pKa = 3.55 YY5112 pKa = 10.54 VYY5114 pKa = 10.83 GYY5116 pKa = 11.06 DD5117 pKa = 3.28 GMRR5120 pKa = 11.84 RR5121 pKa = 11.84 GATYY5125 pKa = 10.18 KK5126 pKa = 10.49 VEE5128 pKa = 4.36 EE5129 pKa = 4.85 DD5130 pKa = 3.18 TDD5132 pKa = 3.95 QNPNTALVVTKK5143 pKa = 10.69 YY5144 pKa = 10.81 EE5145 pKa = 4.09 EE5146 pKa = 3.61 TSYY5149 pKa = 11.42 LVDD5152 pKa = 3.21 SMNFTGYY5159 pKa = 9.29 EE5160 pKa = 3.91 QVVEE5164 pKa = 4.58 EE5165 pKa = 4.22 ITRR5168 pKa = 11.84 DD5169 pKa = 3.33 VATQNVTNAKK5179 pKa = 10.18 LFTLGHH5185 pKa = 6.32 DD5186 pKa = 4.47 VIDD5189 pKa = 3.44 QTTFVPGTQTAGSALTLLYY5208 pKa = 10.33 DD5209 pKa = 3.55 GHH5211 pKa = 6.84 GSTRR5215 pKa = 11.84 AVVDD5219 pKa = 3.63 DD5220 pKa = 5.68 AIAILEE5226 pKa = 4.28 RR5227 pKa = 11.84 YY5228 pKa = 9.72 AYY5230 pKa = 8.04 TAYY5233 pKa = 10.5 GLPIGFDD5240 pKa = 3.08 QAAALTALQYY5250 pKa = 10.8 SGEE5253 pKa = 4.05 LHH5255 pKa = 6.88 DD5256 pKa = 6.08 VRR5258 pKa = 11.84 IGWDD5262 pKa = 3.32 YY5263 pKa = 11.38 LRR5265 pKa = 11.84 RR5266 pKa = 11.84 RR5267 pKa = 11.84 YY5268 pKa = 9.79 YY5269 pKa = 10.44 GPSVGGFNRR5278 pKa = 11.84 LDD5280 pKa = 3.62 DD5281 pKa = 4.24 FAGNMHH5287 pKa = 7.24 DD5288 pKa = 3.62 PQSLHH5293 pKa = 6.62 KK5294 pKa = 10.26 YY5295 pKa = 9.82 AYY5297 pKa = 8.68 THH5299 pKa = 6.95 GDD5301 pKa = 4.06 PINGIDD5307 pKa = 3.68 PTGMANLVTGLKK5319 pKa = 10.26 ALAINAFLTGVAIGRR5334 pKa = 11.84 TILPVAAAAALNVQYY5349 pKa = 10.85 LVYY5352 pKa = 10.2 AHH5354 pKa = 6.99 LWKK5357 pKa = 10.9 VEE5359 pKa = 3.82 MGLVFAYY5366 pKa = 10.43 FGLTVADD5373 pKa = 5.42 AGLSWVDD5380 pKa = 3.31 KK5381 pKa = 10.89 AVTKK5385 pKa = 10.64 SIEE5388 pKa = 3.97 NEE5390 pKa = 3.97 LPHH5393 pKa = 6.62 TDD5395 pKa = 3.18 RR5396 pKa = 11.84 NGGNFFEE5403 pKa = 5.24 KK5404 pKa = 9.91 VTGANGTDD5412 pKa = 3.86 YY5413 pKa = 11.26 QSIDD5417 pKa = 3.33 HH5418 pKa = 7.12 RR5419 pKa = 11.84 EE5420 pKa = 3.84 LDD5422 pKa = 3.47 SKK5424 pKa = 11.29 GRR5426 pKa = 11.84 YY5427 pKa = 5.93 TVVSLGSKK5435 pKa = 10.5 GGDD5438 pKa = 2.87 PLMSEE5443 pKa = 4.18 EE5444 pKa = 4.96 KK5445 pKa = 10.4 FIQRR5449 pKa = 11.84 ALKK5452 pKa = 10.7 EE5453 pKa = 4.15 MGTKK5457 pKa = 9.88 ARR5459 pKa = 11.84 RR5460 pKa = 11.84 ISTNRR5465 pKa = 11.84 QFEE5468 pKa = 4.64 PNPGARR5474 pKa = 11.84 VPALMPGQVGSRR5486 pKa = 11.84 VVLFGIPEE5494 pKa = 4.03 TRR5496 pKa = 11.84 IGIYY5500 pKa = 9.68 QNPDD5504 pKa = 2.81 FVLGLRR5510 pKa = 11.84 MLQRR5514 pKa = 11.84 QFRR5517 pKa = 11.84 NVRR5520 pKa = 11.84 MVIVPLKK5527 pKa = 9.68 GWRR5530 pKa = 11.84 PRR5532 pKa = 3.36
Molecular weight: 596.11 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.689
IPC2_protein 3.694
IPC_protein 3.757
Toseland 3.516
ProMoST 3.923
Dawson 3.757
Bjellqvist 3.897
Wikipedia 3.694
Rodwell 3.579
Grimsley 3.427
Solomon 3.757
Lehninger 3.706
Nozaki 3.859
DTASelect 4.139
Thurlkill 3.567
EMBOSS 3.694
Sillero 3.872
Patrickios 0.935
IPC_peptide 3.745
IPC2_peptide 3.859
IPC2.peptide.svr19 3.798
Protein with the highest isoelectric point:
>tr|A0A517ZJJ8|A0A517ZJJ8_9PLAN Uncharacterized protein OS=Symmachiella dynata OX=2527995 GN=Mal52_11110 PE=4 SV=1
MM1 pKa = 7.83 AKK3 pKa = 8.26 TQRR6 pKa = 11.84 KK7 pKa = 8.43 LKK9 pKa = 9.77 KK10 pKa = 8.85 ANHH13 pKa = 5.94 GARR16 pKa = 11.84 PASSKK21 pKa = 10.39 ARR23 pKa = 11.84 KK24 pKa = 9.19 AKK26 pKa = 9.88 RR27 pKa = 11.84 AKK29 pKa = 10.58 LKK31 pKa = 9.57 TT32 pKa = 3.52
Molecular weight: 3.56 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.361
IPC2_protein 10.847
IPC_protein 12.325
Toseland 12.515
ProMoST 12.983
Dawson 12.53
Bjellqvist 12.501
Wikipedia 12.983
Rodwell 12.574
Grimsley 12.559
Solomon 12.998
Lehninger 12.896
Nozaki 12.515
DTASelect 12.501
Thurlkill 12.515
EMBOSS 13.013
Sillero 12.515
Patrickios 12.31
IPC_peptide 12.998
IPC2_peptide 11.974
IPC2.peptide.svr19 8.937
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6115
0
6115
2198892
30
6482
359.6
39.73
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.695 ± 0.035
1.161 ± 0.013
6.092 ± 0.033
6.324 ± 0.027
3.828 ± 0.019
7.597 ± 0.03
2.291 ± 0.015
5.208 ± 0.02
3.881 ± 0.031
9.858 ± 0.032
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.232 ± 0.016
3.251 ± 0.017
5.246 ± 0.02
4.186 ± 0.027
6.406 ± 0.028
5.841 ± 0.019
5.691 ± 0.031
7.105 ± 0.029
1.543 ± 0.015
2.564 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here