Amino acid dipepetide frequency for Ectocarpus sp. CCAP 1310/34

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	20.671AlaAla: 20.671 ± 0.173	1.641AlaCys: 1.641 ± 0.015	5.122AlaAsp: 5.122 ± 0.031	6.983AlaGlu: 6.983 ± 0.058	2.838AlaPhe: 2.838 ± 0.019	10.572AlaGly: 10.572 ± 0.05	1.712AlaHis: 1.712 ± 0.014	3.107AlaIle: 3.107 ± 0.022	4.383AlaLys: 4.383 ± 0.029	8.066AlaLeu: 8.066 ± 0.039	2.288AlaMet: 2.288 ± 0.014	2.511AlaAsn: 2.511 ± 0.016	5.732AlaPro: 5.732 ± 0.049	2.823AlaGln: 2.823 ± 0.017	7.107AlaArg: 7.107 ± 0.039	8.542AlaSer: 8.542 ± 0.039	6.529AlaThr: 6.529 ± 0.033	7.839AlaVal: 7.839 ± 0.04	1.256AlaTrp: 1.256 ± 0.012	1.578AlaTyr: 1.578 ± 0.014	0.038AlaXaa: 0.038 ± 0.002
Cys	1.349CysAla: 1.349 ± 0.013	0.477CysCys: 0.477 ± 0.009	0.857CysAsp: 0.857 ± 0.011	0.81CysGlu: 0.81 ± 0.01	0.706CysPhe: 0.706 ± 0.008	1.607CysGly: 1.607 ± 0.013	0.395CysHis: 0.395 ± 0.006	0.546CysIle: 0.546 ± 0.008	0.586CysLys: 0.586 ± 0.008	1.397CysLeu: 1.397 ± 0.015	0.384CysMet: 0.384 ± 0.006	0.485CysAsn: 0.485 ± 0.007	0.886CysPro: 0.886 ± 0.014	0.438CysGln: 0.438 ± 0.007	1.172CysArg: 1.172 ± 0.011	1.433CysSer: 1.433 ± 0.014	0.924CysThr: 0.924 ± 0.011	1.154CysVal: 1.154 ± 0.013	0.238CysTrp: 0.238 ± 0.005	0.354CysTyr: 0.354 ± 0.007	0.006CysXaa: 0.006 ± 0.001
Asp	5.43AspAla: 5.43 ± 0.038	0.803AspCys: 0.803 ± 0.01	4.689AspAsp: 4.689 ± 0.04	4.238AspGlu: 4.238 ± 0.03	1.751AspPhe: 1.751 ± 0.017	6.386AspGly: 6.386 ± 0.032	1.056AspHis: 1.056 ± 0.011	1.943AspIle: 1.943 ± 0.017	2.152AspLys: 2.152 ± 0.016	4.142AspLeu: 4.142 ± 0.022	1.177AspMet: 1.177 ± 0.01	1.677AspAsn: 1.677 ± 0.014	2.646AspPro: 2.646 ± 0.019	1.481AspGln: 1.481 ± 0.013	3.364AspArg: 3.364 ± 0.021	3.689AspSer: 3.689 ± 0.024	2.603AspThr: 2.603 ± 0.021	4.138AspVal: 4.138 ± 0.041	0.66AspTrp: 0.66 ± 0.008	1.072AspTyr: 1.072 ± 0.011	0.017AspXaa: 0.017 ± 0.001
Glu	7.491GluAla: 7.491 ± 0.054	0.919GluCys: 0.919 ± 0.011	4.364GluAsp: 4.364 ± 0.029	7.323GluGlu: 7.323 ± 0.061	1.671GluPhe: 1.671 ± 0.014	6.361GluGly: 6.361 ± 0.035	1.308GluHis: 1.308 ± 0.012	2.007GluIle: 2.007 ± 0.016	3.025GluLys: 3.025 ± 0.023	4.913GluLeu: 4.913 ± 0.028	1.443GluMet: 1.443 ± 0.013	1.636GluAsn: 1.636 ± 0.012	2.395GluPro: 2.395 ± 0.018	2.568GluGln: 2.568 ± 0.022	4.86GluArg: 4.86 ± 0.03	3.889GluSer: 3.889 ± 0.021	3.274GluThr: 3.274 ± 0.018	4.312GluVal: 4.312 ± 0.022	0.839GluTrp: 0.839 ± 0.009	1.203GluTyr: 1.203 ± 0.01	0.025GluXaa: 0.025 ± 0.002
Phe	2.457PheAla: 2.457 ± 0.013	0.657PheCys: 0.657 ± 0.01	1.835PheAsp: 1.835 ± 0.018	1.683PheGlu: 1.683 ± 0.014	1.232PhePhe: 1.232 ± 0.013	2.584PheGly: 2.584 ± 0.021	0.79PheHis: 0.79 ± 0.01	0.951PheIle: 0.951 ± 0.009	1.279PheLys: 1.279 ± 0.012	2.722PheLeu: 2.722 ± 0.016	0.675PheMet: 0.675 ± 0.009	0.964PheAsn: 0.964 ± 0.013	1.442PhePro: 1.442 ± 0.014	0.905PheGln: 0.905 ± 0.009	2.027PheArg: 2.027 ± 0.016	2.295PheSer: 2.295 ± 0.017	1.553PheThr: 1.553 ± 0.014	2.31PheVal: 2.31 ± 0.016	0.363PheTrp: 0.363 ± 0.006	0.612PheTyr: 0.612 ± 0.009	0.009PheXaa: 0.009 ± 0.001
Gly	9.598GlyAla: 9.598 ± 0.046	1.626GlyCys: 1.626 ± 0.014	5.99GlyAsp: 5.99 ± 0.034	6.165GlyGlu: 6.165 ± 0.026	2.535GlyPhe: 2.535 ± 0.019	18.813GlyGly: 18.813 ± 0.161	1.862GlyHis: 1.862 ± 0.016	2.748GlyIle: 2.748 ± 0.02	4.182GlyLys: 4.182 ± 0.026	6.237GlyLeu: 6.237 ± 0.025	2.094GlyMet: 2.094 ± 0.016	2.679GlyAsn: 2.679 ± 0.017	3.637GlyPro: 3.637 ± 0.025	2.693GlyGln: 2.693 ± 0.017	7.36GlyArg: 7.36 ± 0.039	8.034GlySer: 8.034 ± 0.042	4.714GlyThr: 4.714 ± 0.038	6.621GlyVal: 6.621 ± 0.03	1.216GlyTrp: 1.216 ± 0.011	1.686GlyTyr: 1.686 ± 0.015	0.075GlyXaa: 0.075 ± 0.003
His	2.081HisAla: 2.081 ± 0.016	0.386HisCys: 0.386 ± 0.006	1.07HisAsp: 1.07 ± 0.009	1.175HisGlu: 1.175 ± 0.011	0.728HisPhe: 0.728 ± 0.008	1.887HisGly: 1.887 ± 0.013	0.791HisHis: 0.791 ± 0.011	0.688HisIle: 0.688 ± 0.008	0.78HisLys: 0.78 ± 0.011	1.88HisLeu: 1.88 ± 0.016	0.453HisMet: 0.453 ± 0.007	0.618HisAsn: 0.618 ± 0.007	1.288HisPro: 1.288 ± 0.013	0.897HisGln: 0.897 ± 0.012	1.746HisArg: 1.746 ± 0.013	1.392HisSer: 1.392 ± 0.011	0.964HisThr: 0.964 ± 0.01	1.503HisVal: 1.503 ± 0.013	0.288HisTrp: 0.288 ± 0.005	0.427HisTyr: 0.427 ± 0.007	0.007HisXaa: 0.007 ± 0.001
Ile	2.837IleAla: 2.837 ± 0.02	0.499IleCys: 0.499 ± 0.007	1.839IleAsp: 1.839 ± 0.015	1.809IleGlu: 1.809 ± 0.016	1.026IlePhe: 1.026 ± 0.011	2.336IleGly: 2.336 ± 0.018	0.613IleHis: 0.613 ± 0.008	1.067IleIle: 1.067 ± 0.012	1.425IleLys: 1.425 ± 0.013	2.519IleLeu: 2.519 ± 0.017	0.689IleMet: 0.689 ± 0.008	0.951IleAsn: 0.951 ± 0.012	1.697IlePro: 1.697 ± 0.013	1.036IleGln: 1.036 ± 0.012	1.916IleArg: 1.916 ± 0.016	2.33IleSer: 2.33 ± 0.018	1.65IleThr: 1.65 ± 0.014	2.234IleVal: 2.234 ± 0.018	0.356IleTrp: 0.356 ± 0.006	0.618IleTyr: 0.618 ± 0.008	0.01IleXaa: 0.01 ± 0.001
Lys	4.654LysAla: 4.654 ± 0.032	0.585LysCys: 0.585 ± 0.01	2.261LysAsp: 2.261 ± 0.018	3.369LysGlu: 3.369 ± 0.027	1.109LysPhe: 1.109 ± 0.011	3.664LysGly: 3.664 ± 0.023	1.027LysHis: 1.027 ± 0.012	1.465LysIle: 1.465 ± 0.014	3.169LysLys: 3.169 ± 0.033	3.324LysLeu: 3.324 ± 0.021	1.031LysMet: 1.031 ± 0.01	1.255LysAsn: 1.255 ± 0.011	1.902LysPro: 1.902 ± 0.017	1.664LysGln: 1.664 ± 0.015	3.663LysArg: 3.663 ± 0.027	2.67LysSer: 2.67 ± 0.018	2.235LysThr: 2.235 ± 0.018	2.915LysVal: 2.915 ± 0.021	0.643LysTrp: 0.643 ± 0.009	0.873LysTyr: 0.873 ± 0.01	0.02LysXaa: 0.02 ± 0.001
Leu	8.013LeuAla: 8.013 ± 0.037	1.386LeuCys: 1.386 ± 0.012	4.285LeuAsp: 4.285 ± 0.022	5.485LeuGlu: 5.485 ± 0.03	2.566LeuPhe: 2.566 ± 0.02	6.107LeuGly: 6.107 ± 0.025	1.893LeuHis: 1.893 ± 0.014	1.982LeuIle: 1.982 ± 0.016	3.489LeuLys: 3.489 ± 0.022	7.766LeuLeu: 7.766 ± 0.04	1.695LeuMet: 1.695 ± 0.015	2.052LeuAsn: 2.052 ± 0.015	4.778LeuPro: 4.778 ± 0.028	3.127LeuGln: 3.127 ± 0.019	6.095LeuArg: 6.095 ± 0.032	6.194LeuSer: 6.194 ± 0.026	3.796LeuThr: 3.796 ± 0.022	5.334LeuVal: 5.334 ± 0.025	1.129LeuTrp: 1.129 ± 0.011	1.591LeuTyr: 1.591 ± 0.012	0.034LeuXaa: 0.034 ± 0.002
Met	2.234MetAla: 2.234 ± 0.018	0.399MetCys: 0.399 ± 0.006	1.251MetAsp: 1.251 ± 0.011	1.444MetGlu: 1.444 ± 0.012	0.716MetPhe: 0.716 ± 0.009	1.56MetGly: 1.56 ± 0.013	0.423MetHis: 0.423 ± 0.006	0.655MetIle: 0.655 ± 0.009	0.994MetLys: 0.994 ± 0.011	2.033MetLeu: 2.033 ± 0.015	0.648MetMet: 0.648 ± 0.009	0.57MetAsn: 0.57 ± 0.007	1.114MetPro: 1.114 ± 0.013	0.76MetGln: 0.76 ± 0.009	1.532MetArg: 1.532 ± 0.013	1.679MetSer: 1.679 ± 0.014	1.175MetThr: 1.175 ± 0.011	1.562MetVal: 1.562 ± 0.015	0.37MetTrp: 0.37 ± 0.006	0.432MetTyr: 0.432 ± 0.007	0.007MetXaa: 0.007 ± 0.001
Asn	2.576AsnAla: 2.576 ± 0.017	0.404AsnCys: 0.404 ± 0.007	1.503AsnAsp: 1.503 ± 0.015	1.506AsnGlu: 1.506 ± 0.012	0.828AsnPhe: 0.828 ± 0.008	2.87AsnGly: 2.87 ± 0.021	0.601AsnHis: 0.601 ± 0.008	0.977AsnIle: 0.977 ± 0.009	1.344AsnLys: 1.344 ± 0.012	2.109AsnLeu: 2.109 ± 0.015	0.649AsnMet: 0.649 ± 0.009	1.168AsnAsn: 1.168 ± 0.017	1.567AsnPro: 1.567 ± 0.012	0.867AsnGln: 0.867 ± 0.01	1.793AsnArg: 1.793 ± 0.013	2.067AsnSer: 2.067 ± 0.013	1.49AsnThr: 1.49 ± 0.012	1.773AsnVal: 1.773 ± 0.016	0.313AsnTrp: 0.313 ± 0.005	0.554AsnTyr: 0.554 ± 0.007	0.01AsnXaa: 0.01 ± 0.001
Pro	6.488ProAla: 6.488 ± 0.054	0.769ProCys: 0.769 ± 0.01	2.592ProAsp: 2.592 ± 0.027	3.166ProGlu: 3.166 ± 0.023	1.491ProPhe: 1.491 ± 0.014	4.564ProGly: 4.564 ± 0.03	1.083ProHis: 1.083 ± 0.01	1.427ProIle: 1.427 ± 0.014	1.96ProLys: 1.96 ± 0.015	4.183ProLeu: 4.183 ± 0.025	0.937ProMet: 0.937 ± 0.009	1.193ProAsn: 1.193 ± 0.012	6.077ProPro: 6.077 ± 0.058	1.807ProGln: 1.807 ± 0.015	3.537ProArg: 3.537 ± 0.024	5.177ProSer: 5.177 ± 0.038	3.452ProThr: 3.452 ± 0.03	3.452ProVal: 3.452 ± 0.025	0.632ProTrp: 0.632 ± 0.009	0.842ProTyr: 0.842 ± 0.01	0.027ProXaa: 0.027 ± 0.001
Gln	3.572GlnAla: 3.572 ± 0.024	0.417GlnCys: 0.417 ± 0.007	1.584GlnAsp: 1.584 ± 0.016	2.508GlnGlu: 2.508 ± 0.019	0.717GlnPhe: 0.717 ± 0.009	2.894GlnGly: 2.894 ± 0.02	1.074GlnHis: 1.074 ± 0.01	0.92GlnIle: 0.92 ± 0.01	1.358GlnLys: 1.358 ± 0.014	2.821GlnLeu: 2.821 ± 0.019	0.663GlnMet: 0.663 ± 0.008	0.811GlnAsn: 0.811 ± 0.009	1.936GlnPro: 1.936 ± 0.019	3.614GlnGln: 3.614 ± 0.053	2.949GlnArg: 2.949 ± 0.021	1.979GlnSer: 1.979 ± 0.013	1.511GlnThr: 1.511 ± 0.012	1.983GlnVal: 1.983 ± 0.015	0.424GlnTrp: 0.424 ± 0.007	0.646GlnTyr: 0.646 ± 0.01	0.01GlnXaa: 0.01 ± 0.001
Arg	6.893ArgAla: 6.893 ± 0.037	1.319ArgCys: 1.319 ± 0.013	3.426ArgAsp: 3.426 ± 0.021	5.027ArgGlu: 5.027 ± 0.032	2.065ArgPhe: 2.065 ± 0.017	6.981ArgGly: 6.981 ± 0.037	1.771ArgHis: 1.771 ± 0.015	2.007ArgIle: 2.007 ± 0.014	3.583ArgLys: 3.583 ± 0.024	5.96ArgLeu: 5.96 ± 0.029	1.562ArgMet: 1.562 ± 0.012	1.911ArgAsn: 1.911 ± 0.014	3.635ArgPro: 3.635 ± 0.024	3.017ArgGln: 3.017 ± 0.02	7.769ArgArg: 7.769 ± 0.051	5.572ArgSer: 5.572 ± 0.029	3.47ArgThr: 3.47 ± 0.02	4.572ArgVal: 4.572 ± 0.023	1.18ArgTrp: 1.18 ± 0.012	1.297ArgTyr: 1.297 ± 0.013	0.036ArgXaa: 0.036 ± 0.002
Ser	8.021SerAla: 8.021 ± 0.039	1.23SerCys: 1.23 ± 0.012	3.886SerAsp: 3.886 ± 0.026	3.791SerGlu: 3.791 ± 0.022	2.383SerPhe: 2.383 ± 0.021	8.066SerGly: 8.066 ± 0.046	1.392SerHis: 1.392 ± 0.012	2.205SerIle: 2.205 ± 0.016	3.113SerLys: 3.113 ± 0.024	5.924SerLeu: 5.924 ± 0.032	1.619SerMet: 1.619 ± 0.014	2.183SerAsn: 2.183 ± 0.015	5.085SerPro: 5.085 ± 0.038	2.066SerGln: 2.066 ± 0.012	5.589SerArg: 5.589 ± 0.028	9.889SerSer: 9.889 ± 0.064	4.84SerThr: 4.84 ± 0.023	4.812SerVal: 4.812 ± 0.03	1.016SerTrp: 1.016 ± 0.011	1.3SerTyr: 1.3 ± 0.014	0.027SerXaa: 0.027 ± 0.002
Thr	6.665ThrAla: 6.665 ± 0.035	0.874ThrCys: 0.874 ± 0.009	2.431ThrAsp: 2.431 ± 0.017	2.876ThrGlu: 2.876 ± 0.022	1.626ThrPhe: 1.626 ± 0.015	4.658ThrGly: 4.658 ± 0.037	1.014ThrHis: 1.014 ± 0.01	1.73ThrIle: 1.73 ± 0.016	2.29ThrLys: 2.29 ± 0.017	4.141ThrLeu: 4.141 ± 0.022	1.085ThrMet: 1.085 ± 0.01	1.434ThrAsn: 1.434 ± 0.012	3.817ThrPro: 3.817 ± 0.031	1.423ThrGln: 1.423 ± 0.011	3.42ThrArg: 3.42 ± 0.02	4.449ThrSer: 4.449 ± 0.029	3.957ThrThr: 3.957 ± 0.029	3.729ThrVal: 3.729 ± 0.026	0.652ThrTrp: 0.652 ± 0.008	1.011ThrTyr: 1.011 ± 0.011	0.026ThrXaa: 0.026 ± 0.002
Val	7.534ValAla: 7.534 ± 0.032	1.129ValCys: 1.129 ± 0.011	4.243ValAsp: 4.243 ± 0.028	4.652ValGlu: 4.652 ± 0.03	2.27ValPhe: 2.27 ± 0.018	5.892ValGly: 5.892 ± 0.024	1.417ValHis: 1.417 ± 0.011	1.995ValIle: 1.995 ± 0.016	2.878ValLys: 2.878 ± 0.02	5.884ValLeu: 5.884 ± 0.027	1.537ValMet: 1.537 ± 0.013	1.742ValAsn: 1.742 ± 0.017	3.769ValPro: 3.769 ± 0.036	2.11ValGln: 2.11 ± 0.016	4.573ValArg: 4.573 ± 0.023	4.967ValSer: 4.967 ± 0.036	3.447ValThr: 3.447 ± 0.025	5.765ValVal: 5.765 ± 0.034	0.966ValTrp: 0.966 ± 0.011	1.399ValTyr: 1.399 ± 0.016	0.027ValXaa: 0.027 ± 0.002
Trp	1.168TrpAla: 1.168 ± 0.011	0.272TrpCys: 0.272 ± 0.005	0.731TrpAsp: 0.731 ± 0.009	0.828TrpGlu: 0.828 ± 0.01	0.44TrpPhe: 0.44 ± 0.007	1.083TrpGly: 1.083 ± 0.011	0.325TrpHis: 0.325 ± 0.006	0.364TrpIle: 0.364 ± 0.006	0.733TrpLys: 0.733 ± 0.01	1.05TrpLeu: 1.05 ± 0.013	0.355TrpMet: 0.355 ± 0.006	0.441TrpAsn: 0.441 ± 0.007	0.606TrpPro: 0.606 ± 0.012	0.461TrpGln: 0.461 ± 0.006	1.139TrpArg: 1.139 ± 0.012	0.894TrpSer: 0.894 ± 0.01	0.677TrpThr: 0.677 ± 0.009	0.879TrpVal: 0.879 ± 0.009	0.218TrpTrp: 0.218 ± 0.005	0.322TrpTyr: 0.322 ± 0.006	0.006TrpXaa: 0.006 ± 0.001
Tyr	1.581TyrAla: 1.581 ± 0.013	0.404TyrCys: 0.404 ± 0.007	1.145TyrAsp: 1.145 ± 0.012	0.968TyrGlu: 0.968 ± 0.011	0.641TyrPhe: 0.641 ± 0.008	1.642TyrGly: 1.642 ± 0.014	0.498TyrHis: 0.498 ± 0.007	0.662TyrIle: 0.662 ± 0.009	0.765TyrLys: 0.765 ± 0.01	1.714TyrLeu: 1.714 ± 0.015	0.416TyrMet: 0.416 ± 0.007	0.679TyrAsn: 0.679 ± 0.009	0.829TyrPro: 0.829 ± 0.011	0.625TyrGln: 0.625 ± 0.007	1.418TyrArg: 1.418 ± 0.013	1.274TyrSer: 1.274 ± 0.011	1.033TyrThr: 1.033 ± 0.011	1.29TyrVal: 1.29 ± 0.013	0.229TyrTrp: 0.229 ± 0.005	0.463TyrTyr: 0.463 ± 0.008	0.007TyrXaa: 0.007 ± 0.001
Xaa	0.024XaaAla: 0.024 ± 0.002	0.007XaaCys: 0.007 ± 0.001	0.01XaaAsp: 0.01 ± 0.001	0.019XaaGlu: 0.019 ± 0.001	0.008XaaPhe: 0.008 ± 0.001	0.038XaaGly: 0.038 ± 0.002	0.005XaaHis: 0.005 ± 0.001	0.008XaaIle: 0.008 ± 0.001	0.011XaaLys: 0.011 ± 0.001	0.014XaaLeu: 0.014 ± 0.001	0.159XaaMet: 0.159 ± 0.004	0.007XaaAsn: 0.007 ± 0.001	0.02XaaPro: 0.02 ± 0.001	0.009XaaGln: 0.009 ± 0.001	0.022XaaArg: 0.022 ± 0.001	0.023XaaSer: 0.023 ± 0.002	0.013XaaThr: 0.013 ± 0.001	0.019XaaVal: 0.019 ± 0.001	0.004XaaTrp: 0.004 ± 0.001	0.004XaaTyr: 0.004 ± 0.001	6.339XaaXaa: 6.339 ± 0.249

Statistics based on 25330 proteins (11448718 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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