Streptomyces phage phiSASD1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.53

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 43 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D7NW84|D7NW84_9CAUD Gp15 OS=Streptomyces phage phiSASD1 OX=747763 GN=15 PE=4 SV=1
MM1 pKa = 7.82SDD3 pKa = 4.31LEE5 pKa = 4.22ALGDD9 pKa = 4.08PEE11 pKa = 5.08HH12 pKa = 7.01KK13 pKa = 10.42CDD15 pKa = 5.35DD16 pKa = 3.49SCAPYY21 pKa = 10.65EE22 pKa = 4.13PLTDD26 pKa = 3.71RR27 pKa = 11.84DD28 pKa = 3.91LEE30 pKa = 4.12EE31 pKa = 5.75LEE33 pKa = 4.4EE34 pKa = 4.25SAHH37 pKa = 6.91IDD39 pKa = 3.31TLYY42 pKa = 11.42ADD44 pKa = 4.15LNLVRR49 pKa = 11.84EE50 pKa = 4.51HH51 pKa = 6.7VALLLLALAEE61 pKa = 4.59AEE63 pKa = 4.3VQTVSMGPRR72 pKa = 11.84SIAVLLDD79 pKa = 3.59LSEE82 pKa = 4.81TEE84 pKa = 4.23AQCLLGAGRR93 pKa = 11.84QTPDD97 pKa = 3.89AIVNHH102 pKa = 7.52AINVAGEE109 pKa = 4.03DD110 pKa = 4.52GYY112 pKa = 11.75VGQAMAALFRR122 pKa = 11.84LEE124 pKa = 4.39MLNMTAVDD132 pKa = 4.56DD133 pKa = 4.31EE134 pKa = 5.14GEE136 pKa = 3.92

Molecular weight: 14.68 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D7NW51|D7NW51_9CAUD Gp25 OS=Streptomyces phage phiSASD1 OX=747763 GN=25 PE=4 SV=1
MM1 pKa = 7.51SLNPLSRR8 pKa = 11.84RR9 pKa = 11.84HH10 pKa = 4.2QQKK13 pKa = 9.92RR14 pKa = 11.84AALAEE19 pKa = 3.98ATARR23 pKa = 11.84SVLAEE28 pKa = 3.89WAKK31 pKa = 10.6VSPEE35 pKa = 3.34AVARR39 pKa = 11.84DD40 pKa = 3.59WARR43 pKa = 11.84LLPRR47 pKa = 11.84VTAYY51 pKa = 10.31VQAGQLHH58 pKa = 6.1AAEE61 pKa = 4.84GTQTFMKK68 pKa = 10.27EE69 pKa = 3.87LLGPDD74 pKa = 3.3AEE76 pKa = 4.54AALVVPEE83 pKa = 4.26QFASQTPDD91 pKa = 2.38GRR93 pKa = 11.84NLMGVLARR101 pKa = 11.84AIPTSIRR108 pKa = 11.84SRR110 pKa = 11.84SAGLSPRR117 pKa = 11.84AAMARR122 pKa = 11.84GAAFLDD128 pKa = 3.69MVVRR132 pKa = 11.84TVVADD137 pKa = 3.66TGRR140 pKa = 11.84QADD143 pKa = 3.59QAAMVGTVQVTSYY156 pKa = 10.69VRR158 pKa = 11.84VVEE161 pKa = 5.11LPACSRR167 pKa = 11.84CIILAGRR174 pKa = 11.84EE175 pKa = 3.98YY176 pKa = 10.85SVSQGFLRR184 pKa = 11.84HH185 pKa = 5.84PRR187 pKa = 11.84CDD189 pKa = 3.24CTMEE193 pKa = 4.05PVVKK197 pKa = 10.13RR198 pKa = 11.84RR199 pKa = 11.84KK200 pKa = 10.24APVLTRR206 pKa = 11.84RR207 pKa = 11.84HH208 pKa = 5.47LRR210 pKa = 11.84PYY212 pKa = 10.99DD213 pKa = 3.45SS214 pKa = 3.95

Molecular weight: 23.36 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

43

0

43

10711

57

1051

249.1

27.05

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.109 ± 0.542

0.766 ± 0.155

6.489 ± 0.303

5.499 ± 0.444

2.913 ± 0.248

8.449 ± 0.403

1.671 ± 0.176

3.875 ± 0.209

3.669 ± 0.314

8.851 ± 0.468

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.427 ± 0.182

2.521 ± 0.273

5.48 ± 0.306

3.352 ± 0.224

7.264 ± 0.368

5.91 ± 0.279

6.591 ± 0.311

7.88 ± 0.31

1.737 ± 0.2

2.549 ± 0.197
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski