Amino acid dipepetide frequency for Streptomyces phage phiSASD1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
16.9AlaAla: 16.9 ± 1.968
0.747AlaCys: 0.747 ± 0.318
7.656AlaAsp: 7.656 ± 0.821
8.31AlaGlu: 8.31 ± 1.071
2.894AlaPhe: 2.894 ± 0.433
10.551AlaGly: 10.551 ± 1.003
2.054AlaHis: 2.054 ± 0.374
4.202AlaIle: 4.202 ± 0.481
4.575AlaLys: 4.575 ± 0.602
13.352AlaLeu: 13.352 ± 2.032
2.801AlaMet: 2.801 ± 0.466
2.148AlaAsn: 2.148 ± 0.32
5.602AlaPro: 5.602 ± 0.866
4.202AlaGln: 4.202 ± 0.662
7.563AlaArg: 7.563 ± 0.898
6.816AlaSer: 6.816 ± 0.826
5.789AlaThr: 5.789 ± 0.804
9.337AlaVal: 9.337 ± 0.792
2.054AlaTrp: 2.054 ± 0.461
3.548AlaTyr: 3.548 ± 0.641
0.0AlaXaa: 0.0 ± 0.0
Cys
0.934CysAla: 0.934 ± 0.302
0.093CysCys: 0.093 ± 0.093
0.934CysAsp: 0.934 ± 0.315
0.28CysGlu: 0.28 ± 0.147
0.093CysPhe: 0.093 ± 0.083
1.027CysGly: 1.027 ± 0.279
0.093CysHis: 0.093 ± 0.093
0.373CysIle: 0.373 ± 0.164
0.093CysLys: 0.093 ± 0.093
1.214CysLeu: 1.214 ± 0.265
0.093CysMet: 0.093 ± 0.09
0.093CysAsn: 0.093 ± 0.093
0.84CysPro: 0.84 ± 0.291
0.0CysGln: 0.0 ± 0.0
0.654CysArg: 0.654 ± 0.335
0.187CysSer: 0.187 ± 0.128
0.467CysThr: 0.467 ± 0.194
0.0CysVal: 0.0 ± 0.0
0.093CysTrp: 0.093 ± 0.083
0.093CysTyr: 0.093 ± 0.09
0.0CysXaa: 0.0 ± 0.0
Asp
8.31AspAla: 8.31 ± 1.116
0.84AspCys: 0.84 ± 0.244
4.015AspAsp: 4.015 ± 0.759
3.641AspGlu: 3.641 ± 0.539
1.214AspPhe: 1.214 ± 0.294
5.976AspGly: 5.976 ± 0.746
0.654AspHis: 0.654 ± 0.212
2.428AspIle: 2.428 ± 0.483
2.148AspLys: 2.148 ± 0.529
5.882AspLeu: 5.882 ± 0.632
1.214AspMet: 1.214 ± 0.369
1.587AspAsn: 1.587 ± 0.583
4.015AspPro: 4.015 ± 0.618
2.894AspGln: 2.894 ± 0.51
3.828AspArg: 3.828 ± 0.473
3.548AspSer: 3.548 ± 0.49
3.268AspThr: 3.268 ± 0.492
6.536AspVal: 6.536 ± 0.779
1.12AspTrp: 1.12 ± 0.325
1.774AspTyr: 1.774 ± 0.431
0.0AspXaa: 0.0 ± 0.0
Glu
7.937GluAla: 7.937 ± 1.083
0.28GluCys: 0.28 ± 0.131
4.949GluAsp: 4.949 ± 0.878
3.641GluGlu: 3.641 ± 0.693
1.681GluPhe: 1.681 ± 0.29
4.482GluGly: 4.482 ± 0.691
1.494GluHis: 1.494 ± 0.484
2.334GluIle: 2.334 ± 0.433
1.307GluLys: 1.307 ± 0.285
4.202GluLeu: 4.202 ± 0.603
1.12GluMet: 1.12 ± 0.338
1.12GluAsn: 1.12 ± 0.277
2.428GluPro: 2.428 ± 0.538
1.027GluGln: 1.027 ± 0.263
4.295GluArg: 4.295 ± 0.759
2.708GluSer: 2.708 ± 0.494
2.614GluThr: 2.614 ± 0.63
4.669GluVal: 4.669 ± 0.694
1.12GluTrp: 1.12 ± 0.27
1.587GluTyr: 1.587 ± 0.383
0.0GluXaa: 0.0 ± 0.0
Phe
2.988PheAla: 2.988 ± 0.482
0.0PheCys: 0.0 ± 0.0
1.961PheAsp: 1.961 ± 0.452
1.867PheGlu: 1.867 ± 0.532
0.654PhePhe: 0.654 ± 0.33
3.081PheGly: 3.081 ± 0.595
0.187PheHis: 0.187 ± 0.112
1.027PheIle: 1.027 ± 0.264
1.307PheLys: 1.307 ± 0.385
2.241PheLeu: 2.241 ± 0.512
0.373PheMet: 0.373 ± 0.157
0.747PheAsn: 0.747 ± 0.249
1.961PhePro: 1.961 ± 0.459
1.401PheGln: 1.401 ± 0.291
2.148PheArg: 2.148 ± 0.443
1.494PheSer: 1.494 ± 0.331
2.988PheThr: 2.988 ± 0.518
1.587PheVal: 1.587 ± 0.393
0.56PheTrp: 0.56 ± 0.209
0.56PheTyr: 0.56 ± 0.182
0.0PheXaa: 0.0 ± 0.0
Gly
6.536GlyAla: 6.536 ± 0.728
0.747GlyCys: 0.747 ± 0.239
5.135GlyAsp: 5.135 ± 0.647
4.388GlyGlu: 4.388 ± 0.577
3.922GlyPhe: 3.922 ± 0.607
5.696GlyGly: 5.696 ± 0.692
1.681GlyHis: 1.681 ± 0.404
3.268GlyIle: 3.268 ± 0.765
4.388GlyLys: 4.388 ± 0.605
8.123GlyLeu: 8.123 ± 1.191
2.521GlyMet: 2.521 ± 0.535
2.894GlyAsn: 2.894 ± 0.668
4.388GlyPro: 4.388 ± 0.611
4.202GlyGln: 4.202 ± 0.517
5.042GlyArg: 5.042 ± 0.604
4.855GlySer: 4.855 ± 0.815
7.096GlyThr: 7.096 ± 1.247
6.162GlyVal: 6.162 ± 0.598
1.587GlyTrp: 1.587 ± 0.439
1.867GlyTyr: 1.867 ± 0.492
0.0GlyXaa: 0.0 ± 0.0
His
1.774HisAla: 1.774 ± 0.324
0.093HisCys: 0.093 ± 0.093
1.027HisAsp: 1.027 ± 0.317
0.654HisGlu: 0.654 ± 0.206
1.027HisPhe: 1.027 ± 0.359
1.867HisGly: 1.867 ± 0.447
0.187HisHis: 0.187 ± 0.158
1.401HisIle: 1.401 ± 0.294
0.56HisLys: 0.56 ± 0.215
1.027HisLeu: 1.027 ± 0.28
0.467HisMet: 0.467 ± 0.204
0.373HisAsn: 0.373 ± 0.167
0.654HisPro: 0.654 ± 0.25
0.28HisGln: 0.28 ± 0.158
1.214HisArg: 1.214 ± 0.285
0.747HisSer: 0.747 ± 0.343
0.747HisThr: 0.747 ± 0.213
1.681HisVal: 1.681 ± 0.403
0.56HisTrp: 0.56 ± 0.201
0.373HisTyr: 0.373 ± 0.198
0.0HisXaa: 0.0 ± 0.0
Ile
4.482IleAla: 4.482 ± 0.502
0.187IleCys: 0.187 ± 0.122
2.334IleAsp: 2.334 ± 0.421
1.867IleGlu: 1.867 ± 0.369
1.307IlePhe: 1.307 ± 0.305
3.455IleGly: 3.455 ± 0.572
1.12IleHis: 1.12 ± 0.323
1.494IleIle: 1.494 ± 0.325
1.027IleLys: 1.027 ± 0.275
2.894IleLeu: 2.894 ± 0.63
0.28IleMet: 0.28 ± 0.161
0.56IleAsn: 0.56 ± 0.25
2.521IlePro: 2.521 ± 0.515
1.027IleGln: 1.027 ± 0.358
4.108IleArg: 4.108 ± 0.754
2.428IleSer: 2.428 ± 0.399
3.175IleThr: 3.175 ± 0.564
2.708IleVal: 2.708 ± 0.511
0.84IleTrp: 0.84 ± 0.235
0.934IleTyr: 0.934 ± 0.253
0.0IleXaa: 0.0 ± 0.0
Lys
4.669LysAla: 4.669 ± 0.757
0.467LysCys: 0.467 ± 0.215
2.521LysAsp: 2.521 ± 0.431
1.587LysGlu: 1.587 ± 0.455
0.654LysPhe: 0.654 ± 0.259
2.801LysGly: 2.801 ± 0.599
0.654LysHis: 0.654 ± 0.223
1.027LysIle: 1.027 ± 0.311
0.467LysLys: 0.467 ± 0.205
3.268LysLeu: 3.268 ± 0.589
1.307LysMet: 1.307 ± 0.378
0.654LysAsn: 0.654 ± 0.232
3.361LysPro: 3.361 ± 0.644
0.654LysGln: 0.654 ± 0.256
3.455LysArg: 3.455 ± 0.6
2.428LysSer: 2.428 ± 0.431
2.334LysThr: 2.334 ± 0.434
2.801LysVal: 2.801 ± 0.577
0.747LysTrp: 0.747 ± 0.272
0.747LysTyr: 0.747 ± 0.258
0.0LysXaa: 0.0 ± 0.0
Leu
13.072LeuAla: 13.072 ± 1.048
0.56LeuCys: 0.56 ± 0.191
4.949LeuAsp: 4.949 ± 0.726
5.042LeuGlu: 5.042 ± 0.765
1.774LeuPhe: 1.774 ± 0.381
7.096LeuGly: 7.096 ± 1.29
1.494LeuHis: 1.494 ± 0.294
3.548LeuIle: 3.548 ± 0.637
4.202LeuLys: 4.202 ± 0.834
6.069LeuLeu: 6.069 ± 0.859
2.334LeuMet: 2.334 ± 0.338
2.894LeuAsn: 2.894 ± 0.58
4.762LeuPro: 4.762 ± 0.999
1.494LeuGln: 1.494 ± 0.331
6.443LeuArg: 6.443 ± 0.776
6.256LeuSer: 6.256 ± 0.827
6.536LeuThr: 6.536 ± 1.001
7.19LeuVal: 7.19 ± 1.192
1.401LeuTrp: 1.401 ± 0.377
1.401LeuTyr: 1.401 ± 0.293
0.0LeuXaa: 0.0 ± 0.0
Met
3.548MetAla: 3.548 ± 0.473
0.187MetCys: 0.187 ± 0.173
1.214MetAsp: 1.214 ± 0.252
1.307MetGlu: 1.307 ± 0.241
0.373MetPhe: 0.373 ± 0.154
1.587MetGly: 1.587 ± 0.373
0.28MetHis: 0.28 ± 0.173
0.84MetIle: 0.84 ± 0.3
1.214MetLys: 1.214 ± 0.369
1.214MetLeu: 1.214 ± 0.299
0.56MetMet: 0.56 ± 0.217
1.027MetAsn: 1.027 ± 0.277
1.307MetPro: 1.307 ± 0.29
0.654MetGln: 0.654 ± 0.202
1.307MetArg: 1.307 ± 0.331
2.054MetSer: 2.054 ± 0.369
2.054MetThr: 2.054 ± 0.383
2.708MetVal: 2.708 ± 0.42
0.28MetTrp: 0.28 ± 0.17
0.56MetTyr: 0.56 ± 0.192
0.0MetXaa: 0.0 ± 0.0
Asn
2.428AsnAla: 2.428 ± 0.444
0.28AsnCys: 0.28 ± 0.136
1.214AsnAsp: 1.214 ± 0.345
0.934AsnGlu: 0.934 ± 0.302
0.84AsnPhe: 0.84 ± 0.214
3.268AsnGly: 3.268 ± 0.472
0.934AsnHis: 0.934 ± 0.268
0.56AsnIle: 0.56 ± 0.25
0.56AsnLys: 0.56 ± 0.201
1.681AsnLeu: 1.681 ± 0.382
0.747AsnMet: 0.747 ± 0.228
0.654AsnAsn: 0.654 ± 0.227
1.494AsnPro: 1.494 ± 0.343
0.934AsnGln: 0.934 ± 0.293
1.12AsnArg: 1.12 ± 0.318
1.774AsnSer: 1.774 ± 0.578
1.494AsnThr: 1.494 ± 0.64
2.708AsnVal: 2.708 ± 0.474
0.654AsnTrp: 0.654 ± 0.256
0.934AsnTyr: 0.934 ± 0.289
0.0AsnXaa: 0.0 ± 0.0
Pro
5.229ProAla: 5.229 ± 0.708
0.28ProCys: 0.28 ± 0.14
3.828ProAsp: 3.828 ± 0.711
4.482ProGlu: 4.482 ± 0.676
1.587ProPhe: 1.587 ± 0.351
4.949ProGly: 4.949 ± 0.768
0.373ProHis: 0.373 ± 0.194
2.614ProIle: 2.614 ± 0.644
1.401ProLys: 1.401 ± 0.356
5.509ProLeu: 5.509 ± 0.91
2.054ProMet: 2.054 ± 0.457
1.587ProAsn: 1.587 ± 0.461
1.587ProPro: 1.587 ± 0.445
2.988ProGln: 2.988 ± 1.033
2.708ProArg: 2.708 ± 0.519
3.081ProSer: 3.081 ± 0.556
3.828ProThr: 3.828 ± 0.501
4.108ProVal: 4.108 ± 0.604
0.654ProTrp: 0.654 ± 0.393
1.961ProTyr: 1.961 ± 0.474
0.0ProXaa: 0.0 ± 0.0
Gln
5.509GlnAla: 5.509 ± 0.763
0.28GlnCys: 0.28 ± 0.15
2.801GlnAsp: 2.801 ± 0.341
1.494GlnGlu: 1.494 ± 0.436
1.027GlnPhe: 1.027 ± 0.304
2.708GlnGly: 2.708 ± 0.411
0.467GlnHis: 0.467 ± 0.214
1.401GlnIle: 1.401 ± 0.33
0.934GlnLys: 0.934 ± 0.355
2.334GlnLeu: 2.334 ± 0.569
0.934GlnMet: 0.934 ± 0.274
0.093GlnAsn: 0.093 ± 0.104
2.428GlnPro: 2.428 ± 0.709
0.56GlnGln: 0.56 ± 0.269
3.455GlnArg: 3.455 ± 0.472
1.12GlnSer: 1.12 ± 0.388
2.521GlnThr: 2.521 ± 0.521
2.428GlnVal: 2.428 ± 0.449
0.467GlnTrp: 0.467 ± 0.205
0.56GlnTyr: 0.56 ± 0.28
0.0GlnXaa: 0.0 ± 0.0
Arg
7.096ArgAla: 7.096 ± 1.117
0.84ArgCys: 0.84 ± 0.298
4.295ArgAsp: 4.295 ± 0.483
4.108ArgGlu: 4.108 ± 0.661
2.614ArgPhe: 2.614 ± 0.489
3.828ArgGly: 3.828 ± 0.567
1.494ArgHis: 1.494 ± 0.395
3.361ArgIle: 3.361 ± 0.484
3.455ArgLys: 3.455 ± 0.626
8.03ArgLeu: 8.03 ± 0.883
1.587ArgMet: 1.587 ± 0.396
1.587ArgAsn: 1.587 ± 0.383
3.735ArgPro: 3.735 ± 0.488
2.334ArgGln: 2.334 ± 0.494
7.47ArgArg: 7.47 ± 0.892
4.669ArgSer: 4.669 ± 0.569
3.922ArgThr: 3.922 ± 0.803
4.949ArgVal: 4.949 ± 0.523
1.307ArgTrp: 1.307 ± 0.381
1.961ArgTyr: 1.961 ± 0.386
0.0ArgXaa: 0.0 ± 0.0
Ser
7.376SerAla: 7.376 ± 0.787
0.467SerCys: 0.467 ± 0.178
4.388SerAsp: 4.388 ± 0.695
2.801SerGlu: 2.801 ± 0.586
1.961SerPhe: 1.961 ± 0.399
7.376SerGly: 7.376 ± 0.835
1.027SerHis: 1.027 ± 0.343
2.148SerIle: 2.148 ± 0.433
2.801SerLys: 2.801 ± 0.585
4.108SerLeu: 4.108 ± 0.707
1.494SerMet: 1.494 ± 0.312
1.307SerAsn: 1.307 ± 0.378
3.175SerPro: 3.175 ± 0.563
1.494SerGln: 1.494 ± 0.43
3.922SerArg: 3.922 ± 0.743
2.614SerSer: 2.614 ± 0.537
3.361SerThr: 3.361 ± 0.822
5.042SerVal: 5.042 ± 0.792
0.84SerTrp: 0.84 ± 0.309
1.401SerTyr: 1.401 ± 0.267
0.0SerXaa: 0.0 ± 0.0
Thr
8.03ThrAla: 8.03 ± 0.692
0.654ThrCys: 0.654 ± 0.281
4.108ThrAsp: 4.108 ± 0.621
3.735ThrGlu: 3.735 ± 0.592
2.521ThrPhe: 2.521 ± 0.481
6.349ThrGly: 6.349 ± 0.669
0.654ThrHis: 0.654 ± 0.24
2.054ThrIle: 2.054 ± 0.547
1.961ThrLys: 1.961 ± 0.408
6.443ThrLeu: 6.443 ± 0.756
1.214ThrMet: 1.214 ± 0.358
1.867ThrAsn: 1.867 ± 0.468
3.828ThrPro: 3.828 ± 0.449
2.054ThrGln: 2.054 ± 0.398
2.988ThrArg: 2.988 ± 0.564
4.108ThrSer: 4.108 ± 0.568
3.735ThrThr: 3.735 ± 0.71
6.162ThrVal: 6.162 ± 0.864
0.84ThrTrp: 0.84 ± 0.298
2.614ThrTyr: 2.614 ± 0.582
0.0ThrXaa: 0.0 ± 0.0
Val
8.59ValAla: 8.59 ± 0.733
0.373ValCys: 0.373 ± 0.186
5.135ValAsp: 5.135 ± 0.66
3.268ValGlu: 3.268 ± 0.502
1.587ValPhe: 1.587 ± 0.316
4.015ValGly: 4.015 ± 0.51
1.401ValHis: 1.401 ± 0.315
3.735ValIle: 3.735 ± 0.546
2.988ValLys: 2.988 ± 0.545
6.909ValLeu: 6.909 ± 0.538
1.961ValMet: 1.961 ± 0.435
3.175ValAsn: 3.175 ± 0.588
5.042ValPro: 5.042 ± 0.674
3.735ValGln: 3.735 ± 0.481
6.069ValArg: 6.069 ± 0.717
5.602ValSer: 5.602 ± 0.723
6.816ValThr: 6.816 ± 0.927
4.762ValVal: 4.762 ± 0.668
1.587ValTrp: 1.587 ± 0.442
2.054ValTyr: 2.054 ± 0.518
0.0ValXaa: 0.0 ± 0.0
Trp
2.241TrpAla: 2.241 ± 0.37
0.093TrpCys: 0.093 ± 0.083
0.747TrpAsp: 0.747 ± 0.218
0.747TrpGlu: 0.747 ± 0.197
0.747TrpPhe: 0.747 ± 0.268
1.587TrpGly: 1.587 ± 0.38
0.373TrpHis: 0.373 ± 0.18
0.467TrpIle: 0.467 ± 0.157
0.467TrpLys: 0.467 ± 0.195
1.214TrpLeu: 1.214 ± 0.332
0.467TrpMet: 0.467 ± 0.204
0.28TrpAsn: 0.28 ± 0.167
0.84TrpPro: 0.84 ± 0.298
0.56TrpGln: 0.56 ± 0.176
1.681TrpArg: 1.681 ± 0.4
1.401TrpSer: 1.401 ± 0.307
1.867TrpThr: 1.867 ± 0.651
1.307TrpVal: 1.307 ± 0.344
0.093TrpTrp: 0.093 ± 0.103
0.187TrpTyr: 0.187 ± 0.159
0.0TrpXaa: 0.0 ± 0.0
Tyr
3.455TyrAla: 3.455 ± 0.471
0.187TyrCys: 0.187 ± 0.099
1.681TyrAsp: 1.681 ± 0.41
0.84TyrGlu: 0.84 ± 0.239
0.654TyrPhe: 0.654 ± 0.206
2.801TyrGly: 2.801 ± 0.628
0.093TyrHis: 0.093 ± 0.09
0.467TyrIle: 0.467 ± 0.184
0.84TyrLys: 0.84 ± 0.277
2.801TyrLeu: 2.801 ± 0.439
0.654TyrMet: 0.654 ± 0.179
0.56TyrAsn: 0.56 ± 0.258
0.84TyrPro: 0.84 ± 0.243
1.027TyrGln: 1.027 ± 0.376
3.175TyrArg: 3.175 ± 0.519
1.214TyrSer: 1.214 ± 0.3
1.307TyrThr: 1.307 ± 0.255
1.961TyrVal: 1.961 ± 0.418
0.56TyrTrp: 0.56 ± 0.242
0.373TyrTyr: 0.373 ± 0.183
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 43 proteins (10711 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski