Chroococcidiopsis thermalis (strain PCC 7203)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Cyanobacteria/Melainabacteria group; Cyanobacteria; Chroococcidiopsidales; Chroococcidiopsidaceae; Chroococcidiopsis; Chroococcidiopsis thermalis

Average proteome isoelectric point is 6.5

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5740 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|K9U8K9|K9U8K9_CHRTP Chromophore lyase CpcT/CpeT OS=Chroococcidiopsis thermalis (strain PCC 7203) OX=251229 GN=cpcT PE=3 SV=1
MM1 pKa = 7.53ANIFGTNLDD10 pKa = 3.88DD11 pKa = 4.06TLTGTDD17 pKa = 3.67SNDD20 pKa = 3.59RR21 pKa = 11.84IYY23 pKa = 11.36GKK25 pKa = 10.26AGKK28 pKa = 8.48DD29 pKa = 3.5TISGLGGDD37 pKa = 3.8DD38 pKa = 3.42RR39 pKa = 11.84LYY41 pKa = 11.36GGAGDD46 pKa = 4.94DD47 pKa = 4.17SLSGGDD53 pKa = 5.42GNDD56 pKa = 3.76TLCGDD61 pKa = 4.51EE62 pKa = 5.84DD63 pKa = 4.19NDD65 pKa = 4.3SLGGGSGNDD74 pKa = 3.39TLYY77 pKa = 11.21GGSGNDD83 pKa = 3.42KK84 pKa = 10.94LYY86 pKa = 11.3GGDD89 pKa = 4.91GNDD92 pKa = 3.58TLQGGNGNDD101 pKa = 3.8YY102 pKa = 10.97IEE104 pKa = 4.99GGFGEE109 pKa = 4.29DD110 pKa = 3.95VIYY113 pKa = 10.53GGQGDD118 pKa = 4.24DD119 pKa = 4.74VIVGDD124 pKa = 4.33LDD126 pKa = 3.78DD127 pKa = 3.77YY128 pKa = 11.22RR129 pKa = 11.84IRR131 pKa = 11.84TGDD134 pKa = 3.04SDD136 pKa = 4.75RR137 pKa = 11.84IYY139 pKa = 11.13GGAGDD144 pKa = 3.8DD145 pKa = 4.25SIYY148 pKa = 11.05GRR150 pKa = 11.84YY151 pKa = 10.21GNDD154 pKa = 3.28SIYY157 pKa = 11.11GDD159 pKa = 5.06DD160 pKa = 5.32GDD162 pKa = 5.8DD163 pKa = 3.75LLAGDD168 pKa = 5.17GYY170 pKa = 11.5GVISGPDD177 pKa = 3.12YY178 pKa = 11.13GVRR181 pKa = 11.84KK182 pKa = 9.91DD183 pKa = 3.97DD184 pKa = 3.46NDD186 pKa = 4.09RR187 pKa = 11.84IYY189 pKa = 11.13GGNGKK194 pKa = 10.01DD195 pKa = 3.08ILVGEE200 pKa = 4.9FGSDD204 pKa = 3.46VLDD207 pKa = 4.41GGSGDD212 pKa = 4.7DD213 pKa = 4.18LLIGAGGGWLRR224 pKa = 11.84GTVDD228 pKa = 3.01SSRR231 pKa = 11.84GDD233 pKa = 3.45SEE235 pKa = 5.77KK236 pKa = 11.11DD237 pKa = 3.27VLTGGSGKK245 pKa = 8.89DD246 pKa = 3.25TFVLAGSGGRR256 pKa = 11.84PGSGTYY262 pKa = 10.07YY263 pKa = 10.58EE264 pKa = 5.41AGDD267 pKa = 4.17SYY269 pKa = 12.28ALITDD274 pKa = 4.35FNKK277 pKa = 10.65YY278 pKa = 9.94EE279 pKa = 4.03DD280 pKa = 4.25TIFLAKK286 pKa = 9.59TNRR289 pKa = 11.84PVTEE293 pKa = 3.69SQYY296 pKa = 10.61IIEE299 pKa = 4.52YY300 pKa = 10.24SLGAAPEE307 pKa = 4.13GLPAGTAIYY316 pKa = 10.19ANNVGEE322 pKa = 4.28TQPNLIAILQGISPDD337 pKa = 4.11SINLNASYY345 pKa = 10.3FKK347 pKa = 10.76ISDD350 pKa = 3.34EE351 pKa = 4.15YY352 pKa = 11.42VRR354 pKa = 11.84YY355 pKa = 10.17SS356 pKa = 3.04

Molecular weight: 36.77 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|K9TU67|K9TU67_CHRTP Conserved TM helix repeat-containing protein OS=Chroococcidiopsis thermalis (strain PCC 7203) OX=251229 GN=Chro_0559 PE=4 SV=1
MM1 pKa = 7.39KK2 pKa = 10.24KK3 pKa = 9.84INRR6 pKa = 11.84FTKK9 pKa = 10.13SQNLALRR16 pKa = 11.84LFIILLLTAIAIWVLRR32 pKa = 11.84GLGIITFLPGGIIWILLLVAIAAGVFSRR60 pKa = 11.84FQRR63 pKa = 11.84RR64 pKa = 11.84WVRR67 pKa = 11.84FF68 pKa = 3.37

Molecular weight: 7.84 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5740

0

5740

1835617

30

2583

319.8

35.56

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.889 ± 0.032

1.01 ± 0.011

4.8 ± 0.024

6.009 ± 0.03

3.927 ± 0.023

6.759 ± 0.033

1.849 ± 0.015

6.515 ± 0.023

4.279 ± 0.028

10.972 ± 0.038

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.872 ± 0.013

3.875 ± 0.026

4.756 ± 0.024

5.502 ± 0.03

5.665 ± 0.026

6.351 ± 0.024

5.687 ± 0.022

6.801 ± 0.024

1.453 ± 0.016

3.028 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski