Kuraishia capsulata CBS 1993
Average proteome isoelectric point is 6.32
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 6027 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|W6MNM3|W6MNM3_9ASCO Uncharacterized protein OS=Kuraishia capsulata CBS 1993 OX=1382522 GN=KUCA_T00002625001 PE=4 SV=1
MM1 pKa = 7.91 PYY3 pKa = 10.81 DD4 pKa = 4.38 DD5 pKa = 6.03 DD6 pKa = 7.31 DD7 pKa = 4.5 YY8 pKa = 12.04 VDD10 pKa = 5.04 YY11 pKa = 11.38 EE12 pKa = 4.37 NEE14 pKa = 4.54 DD15 pKa = 3.52 YY16 pKa = 11.24 DD17 pKa = 4.8 DD18 pKa = 4.58 SEE20 pKa = 4.49 FVEE23 pKa = 5.74 DD24 pKa = 4.21 NLNDD28 pKa = 3.3 EE29 pKa = 5.54 DD30 pKa = 4.37 YY31 pKa = 11.58 ALLYY35 pKa = 7.62 EE36 pKa = 4.44 TLPVLKK42 pKa = 10.41 DD43 pKa = 3.14 QLGDD47 pKa = 3.74 YY48 pKa = 10.93 NSDD51 pKa = 3.4 IQDD54 pKa = 3.46 EE55 pKa = 4.2 DD56 pKa = 3.97 LKK58 pKa = 11.49 AVLYY62 pKa = 10.1 YY63 pKa = 11.11 NYY65 pKa = 10.52 FEE67 pKa = 4.58 VDD69 pKa = 2.9 NALAEE74 pKa = 4.11 IRR76 pKa = 11.84 SKK78 pKa = 10.88 FKK80 pKa = 10.67 RR81 pKa = 11.84 KK82 pKa = 9.44 RR83 pKa = 11.84 GKK85 pKa = 9.72 FDD87 pKa = 3.75 SVFSRR92 pKa = 11.84 LFDD95 pKa = 4.01 FSFDD99 pKa = 3.76 SPGICVDD106 pKa = 3.41
Molecular weight: 12.6 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.728
IPC2_protein 3.706
IPC_protein 3.719
Toseland 3.49
ProMoST 3.859
Dawson 3.719
Bjellqvist 3.91
Wikipedia 3.681
Rodwell 3.541
Grimsley 3.389
Solomon 3.706
Lehninger 3.668
Nozaki 3.834
DTASelect 4.101
Thurlkill 3.554
EMBOSS 3.681
Sillero 3.834
Patrickios 1.875
IPC_peptide 3.706
IPC2_peptide 3.808
IPC2.peptide.svr19 3.787
Protein with the highest isoelectric point:
>tr|W6MW16|W6MW16_9ASCO Histone H2B OS=Kuraishia capsulata CBS 1993 OX=1382522 GN=KUCA_T00002754001 PE=3 SV=1
MM1 pKa = 6.81 YY2 pKa = 8.09 WKK4 pKa = 10.13 PYY6 pKa = 8.95 TDD8 pKa = 4.23 LDD10 pKa = 4.33 RR11 pKa = 11.84 SFSWVFCSSHH21 pKa = 6.95 SCFFWWFWQLSPSPALQMQALPRR44 pKa = 11.84 KK45 pKa = 8.38 WRR47 pKa = 11.84 ILKK50 pKa = 9.62 PCTNVTGKK58 pKa = 8.58 TGRR61 pKa = 11.84 KK62 pKa = 8.34 YY63 pKa = 10.76 RR64 pKa = 11.84 NGDD67 pKa = 3.18 RR68 pKa = 11.84 GPFVLLQLRR77 pKa = 11.84 LFSLFLHH84 pKa = 6.46 IWSSRR89 pKa = 11.84 TCQSNMQGLSRR100 pKa = 11.84 TQSSMHH106 pKa = 6.24 LPP108 pKa = 3.67
Molecular weight: 12.9 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.348
IPC2_protein 9.736
IPC_protein 10.467
Toseland 10.599
ProMoST 10.292
Dawson 10.73
Bjellqvist 10.452
Wikipedia 10.921
Rodwell 10.994
Grimsley 10.774
Solomon 10.818
Lehninger 10.789
Nozaki 10.628
DTASelect 10.423
Thurlkill 10.613
EMBOSS 11.008
Sillero 10.657
Patrickios 10.76
IPC_peptide 10.818
IPC2_peptide 9.736
IPC2.peptide.svr19 8.38
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
6027
0
6027
2778337
10
4949
461.0
51.63
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.747 ± 0.029
1.194 ± 0.011
5.878 ± 0.023
6.783 ± 0.034
4.47 ± 0.02
5.778 ± 0.031
2.039 ± 0.012
5.674 ± 0.017
6.407 ± 0.026
9.735 ± 0.032
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.128 ± 0.011
4.379 ± 0.019
4.673 ± 0.023
3.826 ± 0.022
4.848 ± 0.023
8.943 ± 0.033
5.594 ± 0.02
6.566 ± 0.022
1.124 ± 0.01
3.208 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here