Bacillus phage Waukesha92

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Waukeshavirus; Bacillus virus Waukesha92

Average proteome isoelectric point is 6.57

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 72 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A068EMH2|A0A068EMH2_9CAUD Neck appendage protein OS=Bacillus phage Waukesha92 OX=1510440 GN=Waukesha92_53 PE=4 SV=1
MM1 pKa = 7.8RR2 pKa = 11.84DD3 pKa = 3.51VYY5 pKa = 10.15PVKK8 pKa = 10.6YY9 pKa = 9.46YY10 pKa = 10.69SKK12 pKa = 10.63EE13 pKa = 3.8GFVEE17 pKa = 4.65RR18 pKa = 11.84KK19 pKa = 9.65IEE21 pKa = 4.0INEE24 pKa = 3.74EE25 pKa = 3.85DD26 pKa = 3.64LQKK29 pKa = 10.63IIEE32 pKa = 4.93DD33 pKa = 3.75YY34 pKa = 11.13LDD36 pKa = 3.2RR37 pKa = 11.84HH38 pKa = 6.72ADD40 pKa = 3.28FDD42 pKa = 4.58FDD44 pKa = 3.78EE45 pKa = 5.71LEE47 pKa = 4.41VVNNRR52 pKa = 11.84PMNIWLYY59 pKa = 9.89AKK61 pKa = 9.81CRR63 pKa = 11.84KK64 pKa = 9.51YY65 pKa = 10.86VDD67 pKa = 3.82EE68 pKa = 5.29EE69 pKa = 4.29NPEE72 pKa = 4.11DD73 pKa = 3.85DD74 pKa = 4.03QAVHH78 pKa = 5.64EE79 pKa = 5.06AEE81 pKa = 4.69LEE83 pKa = 4.0ITGDD87 pKa = 3.45YY88 pKa = 10.69DD89 pKa = 4.18GNGIFF94 pKa = 5.19

Molecular weight: 11.3 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A068EM43|A0A068EM43_9CAUD Uncharacterized protein OS=Bacillus phage Waukesha92 OX=1510440 GN=Waukesha92_66 PE=4 SV=1
MM1 pKa = 7.69SMKK4 pKa = 10.14FQSNRR9 pKa = 11.84AAVMARR15 pKa = 11.84HH16 pKa = 6.01LAAKK20 pKa = 9.57KK21 pKa = 9.61AAHH24 pKa = 5.72TAVGQFVSSKK34 pKa = 10.78AKK36 pKa = 10.47LLAAVDD42 pKa = 3.87TGNLRR47 pKa = 11.84SSISSKK53 pKa = 11.32AEE55 pKa = 3.67LEE57 pKa = 4.12KK58 pKa = 11.22VVIGTSADD66 pKa = 3.19YY67 pKa = 10.84GIYY70 pKa = 9.53VEE72 pKa = 5.28KK73 pKa = 10.12GTGVYY78 pKa = 10.35AVDD81 pKa = 3.37GDD83 pKa = 4.07GRR85 pKa = 11.84KK86 pKa = 7.31TPWMYY91 pKa = 11.02RR92 pKa = 11.84DD93 pKa = 3.52PKK95 pKa = 8.09TGKK98 pKa = 8.46MVKK101 pKa = 8.29TQGQHH106 pKa = 4.99AQPFLRR112 pKa = 11.84PAAEE116 pKa = 4.21NNKK119 pKa = 9.76PMITQAATRR128 pKa = 11.84TYY130 pKa = 10.64SSLMRR135 pKa = 5.16

Molecular weight: 14.64 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

72

0

72

13048

48

1098

181.2

20.71

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.461 ± 0.563

0.912 ± 0.131

5.97 ± 0.246

8.438 ± 0.337

4.077 ± 0.225

6.078 ± 0.41

1.387 ± 0.11

7.051 ± 0.217

9.442 ± 0.339

7.84 ± 0.32

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.066 ± 0.218

5.342 ± 0.168

2.859 ± 0.15

4.131 ± 0.201

4.499 ± 0.317

5.388 ± 0.17

5.357 ± 0.335

6.576 ± 0.258

1.418 ± 0.116

3.709 ± 0.317
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski