Amino acid dipepetide frequency for Bacillus phage Waukesha92

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.055AlaAla: 6.055 ± 1.484
0.23AlaCys: 0.23 ± 0.115
3.602AlaAsp: 3.602 ± 0.495
6.055AlaGlu: 6.055 ± 0.729
2.759AlaPhe: 2.759 ± 0.465
3.602AlaGly: 3.602 ± 0.653
0.92AlaHis: 0.92 ± 0.288
4.292AlaIle: 4.292 ± 0.647
5.289AlaLys: 5.289 ± 0.63
5.748AlaLeu: 5.748 ± 0.727
1.763AlaMet: 1.763 ± 0.321
3.066AlaAsn: 3.066 ± 0.584
1.226AlaPro: 1.226 ± 0.346
2.299AlaGln: 2.299 ± 0.483
3.296AlaArg: 3.296 ± 0.451
3.526AlaSer: 3.526 ± 0.53
2.913AlaThr: 2.913 ± 0.529
4.522AlaVal: 4.522 ± 0.755
1.303AlaTrp: 1.303 ± 0.307
2.146AlaTyr: 2.146 ± 0.399
0.0AlaXaa: 0.0 ± 0.0
Cys
0.46CysAla: 0.46 ± 0.199
0.153CysCys: 0.153 ± 0.11
1.15CysAsp: 1.15 ± 0.334
0.996CysGlu: 0.996 ± 0.25
0.46CysPhe: 0.46 ± 0.193
0.537CysGly: 0.537 ± 0.212
0.0CysHis: 0.0 ± 0.0
0.383CysIle: 0.383 ± 0.185
0.843CysLys: 0.843 ± 0.245
0.46CysLeu: 0.46 ± 0.191
0.23CysMet: 0.23 ± 0.122
0.843CysAsn: 0.843 ± 0.256
0.23CysPro: 0.23 ± 0.167
0.307CysGln: 0.307 ± 0.14
0.383CysArg: 0.383 ± 0.151
0.383CysSer: 0.383 ± 0.158
0.537CysThr: 0.537 ± 0.209
0.23CysVal: 0.23 ± 0.138
0.077CysTrp: 0.077 ± 0.08
0.46CysTyr: 0.46 ± 0.192
0.0CysXaa: 0.0 ± 0.0
Asp
2.913AspAla: 2.913 ± 0.656
0.307AspCys: 0.307 ± 0.157
3.679AspAsp: 3.679 ± 0.657
4.292AspGlu: 4.292 ± 0.585
2.606AspPhe: 2.606 ± 0.4
4.752AspGly: 4.752 ± 0.747
0.92AspHis: 0.92 ± 0.259
4.369AspIle: 4.369 ± 0.703
5.289AspLys: 5.289 ± 0.508
4.445AspLeu: 4.445 ± 0.644
2.223AspMet: 2.223 ± 0.399
2.683AspAsn: 2.683 ± 0.57
2.146AspPro: 2.146 ± 0.365
2.223AspGln: 2.223 ± 0.396
2.529AspArg: 2.529 ± 0.39
2.606AspSer: 2.606 ± 0.429
3.066AspThr: 3.066 ± 0.476
4.905AspVal: 4.905 ± 0.64
1.073AspTrp: 1.073 ± 0.312
2.683AspTyr: 2.683 ± 0.432
0.0AspXaa: 0.0 ± 0.0
Glu
5.825GluAla: 5.825 ± 0.784
0.537GluCys: 0.537 ± 0.174
4.522GluAsp: 4.522 ± 0.688
7.665GluGlu: 7.665 ± 1.051
3.449GluPhe: 3.449 ± 0.541
4.139GluGly: 4.139 ± 0.502
0.996GluHis: 0.996 ± 0.272
5.825GluIle: 5.825 ± 0.634
8.048GluLys: 8.048 ± 1.012
6.898GluLeu: 6.898 ± 0.798
2.299GluMet: 2.299 ± 0.587
4.292GluAsn: 4.292 ± 0.48
1.993GluPro: 1.993 ± 0.496
4.062GluGln: 4.062 ± 0.501
4.829GluArg: 4.829 ± 0.537
4.752GluSer: 4.752 ± 0.725
4.369GluThr: 4.369 ± 0.584
4.982GluVal: 4.982 ± 0.712
1.993GluTrp: 1.993 ± 0.419
2.913GluTyr: 2.913 ± 0.415
0.0GluXaa: 0.0 ± 0.0
Phe
2.376PheAla: 2.376 ± 0.503
0.383PheCys: 0.383 ± 0.179
2.836PheAsp: 2.836 ± 0.415
2.989PheGlu: 2.989 ± 0.378
1.38PhePhe: 1.38 ± 0.321
3.296PheGly: 3.296 ± 0.61
0.613PheHis: 0.613 ± 0.191
4.139PheIle: 4.139 ± 0.573
3.986PheLys: 3.986 ± 0.512
2.146PheLeu: 2.146 ± 0.45
0.843PheMet: 0.843 ± 0.212
2.376PheAsn: 2.376 ± 0.402
0.843PhePro: 0.843 ± 0.257
1.533PheGln: 1.533 ± 0.352
1.763PheArg: 1.763 ± 0.366
2.453PheSer: 2.453 ± 0.365
2.069PheThr: 2.069 ± 0.441
2.836PheVal: 2.836 ± 0.476
0.537PheTrp: 0.537 ± 0.211
1.38PheTyr: 1.38 ± 0.283
0.0PheXaa: 0.0 ± 0.0
Gly
4.445GlyAla: 4.445 ± 0.946
0.307GlyCys: 0.307 ± 0.185
4.216GlyAsp: 4.216 ± 0.585
4.445GlyGlu: 4.445 ± 0.558
2.069GlyPhe: 2.069 ± 0.449
3.909GlyGly: 3.909 ± 0.798
0.307GlyHis: 0.307 ± 0.148
4.369GlyIle: 4.369 ± 0.906
5.519GlyLys: 5.519 ± 0.639
5.059GlyLeu: 5.059 ± 0.778
2.223GlyMet: 2.223 ± 0.362
2.989GlyAsn: 2.989 ± 0.479
0.996GlyPro: 0.996 ± 0.288
1.84GlyGln: 1.84 ± 0.382
2.913GlyArg: 2.913 ± 0.425
3.372GlySer: 3.372 ± 0.504
3.526GlyThr: 3.526 ± 0.6
3.832GlyVal: 3.832 ± 0.925
1.763GlyTrp: 1.763 ± 0.346
2.683GlyTyr: 2.683 ± 0.49
0.0GlyXaa: 0.0 ± 0.0
His
0.843HisAla: 0.843 ± 0.286
0.307HisCys: 0.307 ± 0.146
0.766HisAsp: 0.766 ± 0.203
0.766HisGlu: 0.766 ± 0.252
0.92HisPhe: 0.92 ± 0.282
0.843HisGly: 0.843 ± 0.234
0.23HisHis: 0.23 ± 0.139
0.69HisIle: 0.69 ± 0.226
1.226HisLys: 1.226 ± 0.293
1.303HisLeu: 1.303 ± 0.293
0.23HisMet: 0.23 ± 0.122
0.307HisAsn: 0.307 ± 0.168
0.23HisPro: 0.23 ± 0.138
0.843HisGln: 0.843 ± 0.275
0.69HisArg: 0.69 ± 0.23
0.766HisSer: 0.766 ± 0.264
0.69HisThr: 0.69 ± 0.228
1.303HisVal: 1.303 ± 0.23
0.307HisTrp: 0.307 ± 0.127
0.613HisTyr: 0.613 ± 0.201
0.0HisXaa: 0.0 ± 0.0
Ile
5.672IleAla: 5.672 ± 0.751
1.38IleCys: 1.38 ± 0.336
4.062IleAsp: 4.062 ± 0.569
6.438IleGlu: 6.438 ± 0.531
2.606IlePhe: 2.606 ± 0.557
4.139IleGly: 4.139 ± 0.619
0.92IleHis: 0.92 ± 0.267
4.752IleIle: 4.752 ± 0.623
6.132IleLys: 6.132 ± 0.839
5.212IleLeu: 5.212 ± 0.672
1.15IleMet: 1.15 ± 0.377
4.675IleAsn: 4.675 ± 0.555
2.299IlePro: 2.299 ± 0.378
2.913IleGln: 2.913 ± 0.447
3.142IleArg: 3.142 ± 0.56
4.216IleSer: 4.216 ± 0.624
4.445IleThr: 4.445 ± 0.597
3.449IleVal: 3.449 ± 0.535
0.843IleTrp: 0.843 ± 0.222
2.069IleTyr: 2.069 ± 0.43
0.0IleXaa: 0.0 ± 0.0
Lys
6.055LysAla: 6.055 ± 0.785
0.537LysCys: 0.537 ± 0.217
5.978LysAsp: 5.978 ± 0.762
9.581LysGlu: 9.581 ± 1.056
2.683LysPhe: 2.683 ± 0.489
4.752LysGly: 4.752 ± 0.589
1.303LysHis: 1.303 ± 0.284
6.285LysIle: 6.285 ± 0.688
9.581LysLys: 9.581 ± 1.374
7.435LysLeu: 7.435 ± 0.734
2.989LysMet: 2.989 ± 0.465
4.905LysAsn: 4.905 ± 0.648
2.836LysPro: 2.836 ± 0.345
4.216LysGln: 4.216 ± 0.563
5.365LysArg: 5.365 ± 0.71
4.292LysSer: 4.292 ± 0.491
4.905LysThr: 4.905 ± 0.633
6.898LysVal: 6.898 ± 0.697
0.537LysTrp: 0.537 ± 0.217
3.296LysTyr: 3.296 ± 0.575
0.0LysXaa: 0.0 ± 0.0
Leu
5.289LeuAla: 5.289 ± 0.597
0.69LeuCys: 0.69 ± 0.215
5.212LeuAsp: 5.212 ± 0.689
6.898LeuGlu: 6.898 ± 0.731
2.836LeuPhe: 2.836 ± 0.494
4.752LeuGly: 4.752 ± 0.82
1.226LeuHis: 1.226 ± 0.294
4.905LeuIle: 4.905 ± 0.823
6.745LeuLys: 6.745 ± 0.794
4.982LeuLeu: 4.982 ± 0.764
2.606LeuMet: 2.606 ± 0.544
4.139LeuAsn: 4.139 ± 0.61
2.299LeuPro: 2.299 ± 0.347
3.449LeuGln: 3.449 ± 0.559
3.296LeuArg: 3.296 ± 0.494
5.748LeuSer: 5.748 ± 0.617
4.216LeuThr: 4.216 ± 0.517
5.595LeuVal: 5.595 ± 0.812
0.69LeuTrp: 0.69 ± 0.224
2.836LeuTyr: 2.836 ± 0.479
0.0LeuXaa: 0.0 ± 0.0
Met
1.993MetAla: 1.993 ± 0.426
0.383MetCys: 0.383 ± 0.179
1.533MetAsp: 1.533 ± 0.335
1.993MetGlu: 1.993 ± 0.418
1.533MetPhe: 1.533 ± 0.385
1.073MetGly: 1.073 ± 0.291
0.46MetHis: 0.46 ± 0.211
1.763MetIle: 1.763 ± 0.349
3.832MetLys: 3.832 ± 0.543
2.146MetLeu: 2.146 ± 0.351
1.073MetMet: 1.073 ± 0.273
2.299MetAsn: 2.299 ± 0.424
1.226MetPro: 1.226 ± 0.352
1.61MetGln: 1.61 ± 0.417
1.61MetArg: 1.61 ± 0.291
1.686MetSer: 1.686 ± 0.368
1.916MetThr: 1.916 ± 0.358
1.303MetVal: 1.303 ± 0.32
0.46MetTrp: 0.46 ± 0.244
0.766MetTyr: 0.766 ± 0.277
0.0MetXaa: 0.0 ± 0.0
Asn
2.989AsnAla: 2.989 ± 0.625
0.766AsnCys: 0.766 ± 0.224
3.756AsnAsp: 3.756 ± 0.623
5.059AsnGlu: 5.059 ± 0.608
1.763AsnPhe: 1.763 ± 0.336
4.752AsnGly: 4.752 ± 0.742
0.69AsnHis: 0.69 ± 0.269
3.832AsnIle: 3.832 ± 0.572
4.599AsnLys: 4.599 ± 0.725
5.672AsnLeu: 5.672 ± 0.597
1.61AsnMet: 1.61 ± 0.343
2.529AsnAsn: 2.529 ± 0.386
1.916AsnPro: 1.916 ± 0.32
1.38AsnGln: 1.38 ± 0.319
1.763AsnArg: 1.763 ± 0.355
2.376AsnSer: 2.376 ± 0.441
2.529AsnThr: 2.529 ± 0.492
3.296AsnVal: 3.296 ± 0.465
0.766AsnTrp: 0.766 ± 0.249
1.38AsnTyr: 1.38 ± 0.37
0.0AsnXaa: 0.0 ± 0.0
Pro
1.84ProAla: 1.84 ± 0.322
0.23ProCys: 0.23 ± 0.132
1.15ProAsp: 1.15 ± 0.294
1.916ProGlu: 1.916 ± 0.466
1.303ProPhe: 1.303 ± 0.416
1.38ProGly: 1.38 ± 0.379
0.613ProHis: 0.613 ± 0.194
1.61ProIle: 1.61 ± 0.419
2.529ProLys: 2.529 ± 0.409
2.069ProLeu: 2.069 ± 0.436
1.073ProMet: 1.073 ± 0.252
1.686ProAsn: 1.686 ± 0.372
0.996ProPro: 0.996 ± 0.306
1.226ProGln: 1.226 ± 0.313
0.613ProArg: 0.613 ± 0.325
1.303ProSer: 1.303 ± 0.29
2.146ProThr: 2.146 ± 0.427
2.989ProVal: 2.989 ± 0.532
0.307ProTrp: 0.307 ± 0.142
1.61ProTyr: 1.61 ± 0.305
0.0ProXaa: 0.0 ± 0.0
Gln
2.529GlnAla: 2.529 ± 0.457
0.307GlnCys: 0.307 ± 0.155
1.61GlnAsp: 1.61 ± 0.32
4.062GlnGlu: 4.062 ± 0.523
2.069GlnPhe: 2.069 ± 0.383
2.299GlnGly: 2.299 ± 0.378
0.46GlnHis: 0.46 ± 0.192
2.606GlnIle: 2.606 ± 0.404
3.832GlnLys: 3.832 ± 0.483
3.296GlnLeu: 3.296 ± 0.551
1.533GlnMet: 1.533 ± 0.351
1.993GlnAsn: 1.993 ± 0.498
1.226GlnPro: 1.226 ± 0.279
2.529GlnGln: 2.529 ± 0.58
2.069GlnArg: 2.069 ± 0.362
2.453GlnSer: 2.453 ± 0.405
1.993GlnThr: 1.993 ± 0.403
1.916GlnVal: 1.916 ± 0.393
0.46GlnTrp: 0.46 ± 0.215
2.069GlnTyr: 2.069 ± 0.36
0.0GlnXaa: 0.0 ± 0.0
Arg
2.606ArgAla: 2.606 ± 0.513
0.46ArgCys: 0.46 ± 0.223
1.993ArgAsp: 1.993 ± 0.289
3.066ArgGlu: 3.066 ± 0.461
2.223ArgPhe: 2.223 ± 0.435
2.606ArgGly: 2.606 ± 0.5
0.766ArgHis: 0.766 ± 0.228
4.062ArgIle: 4.062 ± 0.67
5.442ArgLys: 5.442 ± 0.859
3.679ArgLeu: 3.679 ± 0.59
1.303ArgMet: 1.303 ± 0.288
2.299ArgAsn: 2.299 ± 0.365
0.92ArgPro: 0.92 ± 0.229
1.303ArgGln: 1.303 ± 0.251
1.916ArgArg: 1.916 ± 0.502
2.376ArgSer: 2.376 ± 0.534
2.299ArgThr: 2.299 ± 0.421
3.066ArgVal: 3.066 ± 0.578
0.537ArgTrp: 0.537 ± 0.201
2.069ArgTyr: 2.069 ± 0.487
0.0ArgXaa: 0.0 ± 0.0
Ser
2.913SerAla: 2.913 ± 0.518
0.537SerCys: 0.537 ± 0.193
3.372SerAsp: 3.372 ± 0.492
4.522SerGlu: 4.522 ± 0.497
3.066SerPhe: 3.066 ± 0.503
2.759SerGly: 2.759 ± 0.477
0.46SerHis: 0.46 ± 0.177
4.675SerIle: 4.675 ± 0.679
5.595SerLys: 5.595 ± 0.58
4.292SerLeu: 4.292 ± 0.53
1.61SerMet: 1.61 ± 0.336
3.066SerAsn: 3.066 ± 0.441
1.15SerPro: 1.15 ± 0.241
2.529SerGln: 2.529 ± 0.396
2.223SerArg: 2.223 ± 0.414
2.529SerSer: 2.529 ± 0.43
2.683SerThr: 2.683 ± 0.586
3.602SerVal: 3.602 ± 0.644
0.46SerTrp: 0.46 ± 0.18
1.84SerTyr: 1.84 ± 0.474
0.0SerXaa: 0.0 ± 0.0
Thr
3.296ThrAla: 3.296 ± 0.473
0.307ThrCys: 0.307 ± 0.184
2.606ThrAsp: 2.606 ± 0.549
3.679ThrGlu: 3.679 ± 0.522
2.529ThrPhe: 2.529 ± 0.439
3.219ThrGly: 3.219 ± 0.564
1.15ThrHis: 1.15 ± 0.3
5.059ThrIle: 5.059 ± 0.631
6.362ThrLys: 6.362 ± 0.939
4.292ThrLeu: 4.292 ± 0.668
1.763ThrMet: 1.763 ± 0.325
2.913ThrAsn: 2.913 ± 0.413
2.453ThrPro: 2.453 ± 0.444
2.069ThrGln: 2.069 ± 0.378
1.763ThrArg: 1.763 ± 0.354
1.456ThrSer: 1.456 ± 0.346
2.913ThrThr: 2.913 ± 0.54
3.372ThrVal: 3.372 ± 0.557
0.537ThrTrp: 0.537 ± 0.218
1.84ThrTyr: 1.84 ± 0.388
0.0ThrXaa: 0.0 ± 0.0
Val
3.986ValAla: 3.986 ± 0.487
0.23ValCys: 0.23 ± 0.134
4.139ValAsp: 4.139 ± 0.595
5.748ValGlu: 5.748 ± 0.787
2.606ValPhe: 2.606 ± 0.511
4.675ValGly: 4.675 ± 0.518
1.15ValHis: 1.15 ± 0.305
4.062ValIle: 4.062 ± 0.543
5.289ValLys: 5.289 ± 0.659
4.982ValLeu: 4.982 ± 0.63
2.146ValMet: 2.146 ± 0.439
3.679ValAsn: 3.679 ± 0.546
2.299ValPro: 2.299 ± 0.367
2.913ValGln: 2.913 ± 0.452
2.453ValArg: 2.453 ± 0.509
4.829ValSer: 4.829 ± 0.645
3.909ValThr: 3.909 ± 0.62
3.832ValVal: 3.832 ± 0.534
0.69ValTrp: 0.69 ± 0.255
2.146ValTyr: 2.146 ± 0.391
0.0ValXaa: 0.0 ± 0.0
Trp
0.537TrpAla: 0.537 ± 0.188
0.46TrpCys: 0.46 ± 0.191
1.38TrpAsp: 1.38 ± 0.34
1.533TrpGlu: 1.533 ± 0.362
0.46TrpPhe: 0.46 ± 0.222
1.226TrpGly: 1.226 ± 0.328
0.153TrpHis: 0.153 ± 0.108
0.69TrpIle: 0.69 ± 0.251
1.15TrpLys: 1.15 ± 0.237
0.92TrpLeu: 0.92 ± 0.237
0.613TrpMet: 0.613 ± 0.241
0.92TrpAsn: 0.92 ± 0.223
0.153TrpPro: 0.153 ± 0.121
0.46TrpGln: 0.46 ± 0.207
0.69TrpArg: 0.69 ± 0.216
0.92TrpSer: 0.92 ± 0.259
0.613TrpThr: 0.613 ± 0.205
0.766TrpVal: 0.766 ± 0.258
0.153TrpTrp: 0.153 ± 0.107
0.383TrpTyr: 0.383 ± 0.134
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.993TyrAla: 1.993 ± 0.414
0.613TyrCys: 0.613 ± 0.221
2.146TyrAsp: 2.146 ± 0.426
2.683TyrGlu: 2.683 ± 0.425
1.456TyrPhe: 1.456 ± 0.399
2.069TyrGly: 2.069 ± 0.413
0.537TyrHis: 0.537 ± 0.234
2.146TyrIle: 2.146 ± 0.374
3.142TyrLys: 3.142 ± 0.571
3.372TyrLeu: 3.372 ± 0.667
1.303TyrMet: 1.303 ± 0.301
1.763TyrAsn: 1.763 ± 0.438
1.15TyrPro: 1.15 ± 0.298
1.61TyrGln: 1.61 ± 0.367
1.686TyrArg: 1.686 ± 0.386
1.84TyrSer: 1.84 ± 0.299
1.84TyrThr: 1.84 ± 0.352
3.066TyrVal: 3.066 ± 0.579
0.69TyrTrp: 0.69 ± 0.206
1.916TyrTyr: 1.916 ± 0.535
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 72 proteins (13048 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski