Marine Group I thaumarchaeote SCGC AAA799-B03

Taxonomy: cellular organisms; Archaea; TACK group; Thaumarchaeota; Thaumarchaeota incertae sedis; Marine Group I

Average proteome isoelectric point is 6.45

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1339 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A087S7R3|A0A087S7R3_9ARCH Phosphate transport system permease protein PstA OS=Marine Group I thaumarchaeote SCGC AAA799-B03 OX=1502289 GN=pstA PE=3 SV=1
MM1 pKa = 7.04KK2 pKa = 9.13TLPVIVTTLSVILVSSTLTPSFALGEE28 pKa = 3.94YY29 pKa = 10.06DD30 pKa = 5.24YY31 pKa = 11.46LSEE34 pKa = 3.78WGEE37 pKa = 3.87FGIYY41 pKa = 9.42TPGFFSYY48 pKa = 10.58PEE50 pKa = 4.09YY51 pKa = 10.58IAVDD55 pKa = 3.67EE56 pKa = 5.45DD57 pKa = 4.05GNSYY61 pKa = 10.67ISDD64 pKa = 4.06LGNKK68 pKa = 9.7RR69 pKa = 11.84IQKK72 pKa = 9.96FSSDD76 pKa = 3.35GQYY79 pKa = 9.87ISHH82 pKa = 6.99WGEE85 pKa = 3.75SGKK88 pKa = 9.89QAGQFHH94 pKa = 6.84YY95 pKa = 9.96PSGITISGDD104 pKa = 3.13HH105 pKa = 6.74VYY107 pKa = 10.74VADD110 pKa = 3.86QALHH114 pKa = 7.27RR115 pKa = 11.84IQQFTLDD122 pKa = 4.22GEE124 pKa = 5.18FVSQWGSKK132 pKa = 9.85GIHH135 pKa = 5.78EE136 pKa = 5.08GEE138 pKa = 3.9FKK140 pKa = 10.82YY141 pKa = 10.33PYY143 pKa = 10.24GITSDD148 pKa = 3.86SEE150 pKa = 3.98HH151 pKa = 7.75VYY153 pKa = 10.87VVDD156 pKa = 3.9TGNQRR161 pKa = 11.84IQKK164 pKa = 8.02FTLDD168 pKa = 3.65GEE170 pKa = 4.79FVLSFGTSGMGPGQFLTATGIDD192 pKa = 3.55VEE194 pKa = 4.56DD195 pKa = 4.26DD196 pKa = 3.56VVYY199 pKa = 9.64VTDD202 pKa = 3.59KK203 pKa = 11.3GNRR206 pKa = 11.84KK207 pKa = 8.94IDD209 pKa = 3.58TFDD212 pKa = 3.48SDD214 pKa = 4.12GNFIKK219 pKa = 10.4SYY221 pKa = 9.65PFRR224 pKa = 11.84GLNYY228 pKa = 8.82VFAPEE233 pKa = 5.18GIAVSPEE240 pKa = 3.46GKK242 pKa = 10.36VFVTNSDD249 pKa = 3.71NNKK252 pKa = 9.41VLYY255 pKa = 10.6LGLDD259 pKa = 3.51GDD261 pKa = 4.3LSLDD265 pKa = 3.2IFEE268 pKa = 5.31KK269 pKa = 10.78NGPLKK274 pKa = 10.12HH275 pKa = 6.04TFTSLTDD282 pKa = 3.34VAIGSNGEE290 pKa = 4.11LLVVDD295 pKa = 4.72SAAHH299 pKa = 6.39KK300 pKa = 9.9IKK302 pKa = 10.84SFEE305 pKa = 4.13TPFYY309 pKa = 10.75SEE311 pKa = 5.23PIVKK315 pKa = 9.59EE316 pKa = 3.62QVEE319 pKa = 4.49IVAPEE324 pKa = 3.92VEE326 pKa = 4.28EE327 pKa = 5.66DD328 pKa = 3.93YY329 pKa = 11.58SFDD332 pKa = 5.83DD333 pKa = 3.61IDD335 pKa = 4.11PVIMAPSDD343 pKa = 3.32IKK345 pKa = 11.44LEE347 pKa = 4.17ATDD350 pKa = 4.86LFTPVLIGEE359 pKa = 4.66AVAEE363 pKa = 4.51DD364 pKa = 3.83LHH366 pKa = 8.53SGIKK370 pKa = 10.37AILNNAPEE378 pKa = 4.35AFSLGVSKK386 pKa = 8.65VTWIAFDD393 pKa = 3.71NAGNTAEE400 pKa = 4.94DD401 pKa = 3.89YY402 pKa = 11.59QNITVYY408 pKa = 11.08ACGNVYY414 pKa = 10.65SDD416 pKa = 3.76YY417 pKa = 11.77NMIVGTDD424 pKa = 3.65EE425 pKa = 4.84NDD427 pKa = 3.31VLQGTSGDD435 pKa = 3.78DD436 pKa = 3.96LIFGLEE442 pKa = 4.07GNDD445 pKa = 3.63IISGLEE451 pKa = 3.89GNDD454 pKa = 3.96CIFGGEE460 pKa = 4.19GDD462 pKa = 4.02DD463 pKa = 4.05VVYY466 pKa = 11.08GNDD469 pKa = 4.05GYY471 pKa = 10.58DD472 pKa = 3.41TISGNGGHH480 pKa = 7.72DD481 pKa = 3.2ILKK484 pKa = 9.97GDD486 pKa = 3.61SGFDD490 pKa = 3.45VIYY493 pKa = 10.19GGSGSDD499 pKa = 3.48VLDD502 pKa = 4.12GGSEE506 pKa = 3.96NDD508 pKa = 3.21NCYY511 pKa = 10.86DD512 pKa = 3.71SQDD515 pKa = 4.61SIILNCNEE523 pKa = 3.66

Molecular weight: 56.7 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A087S8E1|A0A087S8E1_9ARCH Uncharacterized protein OS=Marine Group I thaumarchaeote SCGC AAA799-B03 OX=1502289 GN=AAA799B03_00442 PE=4 SV=1
MM1 pKa = 7.42AMSRR5 pKa = 11.84AKK7 pKa = 10.31RR8 pKa = 11.84AEE10 pKa = 3.95AAKK13 pKa = 8.98KK14 pKa = 6.95TARR17 pKa = 11.84TRR19 pKa = 11.84KK20 pKa = 9.58RR21 pKa = 11.84NAAKK25 pKa = 10.3KK26 pKa = 9.8AAEE29 pKa = 4.03AAKK32 pKa = 10.16KK33 pKa = 9.77AAARR37 pKa = 11.84KK38 pKa = 9.21RR39 pKa = 11.84KK40 pKa = 9.17AARR43 pKa = 11.84ARR45 pKa = 11.84NAAKK49 pKa = 10.21KK50 pKa = 9.55AAPKK54 pKa = 10.27RR55 pKa = 11.84RR56 pKa = 11.84TAAKK60 pKa = 9.88RR61 pKa = 11.84KK62 pKa = 8.47AAPKK66 pKa = 10.03RR67 pKa = 11.84RR68 pKa = 11.84TAAKK72 pKa = 9.88RR73 pKa = 11.84KK74 pKa = 8.72AAPKK78 pKa = 10.04RR79 pKa = 11.84KK80 pKa = 7.37TAAKK84 pKa = 9.84RR85 pKa = 11.84KK86 pKa = 8.7AAPKK90 pKa = 10.0RR91 pKa = 11.84KK92 pKa = 9.28AATKK96 pKa = 10.14RR97 pKa = 11.84RR98 pKa = 11.84AAPKK102 pKa = 9.97RR103 pKa = 11.84KK104 pKa = 7.39TAAKK108 pKa = 9.84RR109 pKa = 11.84KK110 pKa = 8.7AAPKK114 pKa = 10.0RR115 pKa = 11.84KK116 pKa = 9.28AATKK120 pKa = 10.14RR121 pKa = 11.84RR122 pKa = 11.84AAPKK126 pKa = 9.76RR127 pKa = 11.84KK128 pKa = 8.49AAKK131 pKa = 9.71RR132 pKa = 11.84RR133 pKa = 11.84RR134 pKa = 3.63

Molecular weight: 14.77 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1339

0

1339

297946

23

1263

222.5

25.01

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.901 ± 0.082

1.075 ± 0.033

5.84 ± 0.068

7.408 ± 0.081

4.438 ± 0.061

6.407 ± 0.083

1.849 ± 0.031

8.676 ± 0.059

8.773 ± 0.091

8.486 ± 0.067

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.639 ± 0.041

4.978 ± 0.071

3.73 ± 0.051

3.231 ± 0.042

3.599 ± 0.05

7.002 ± 0.063

5.462 ± 0.05

6.443 ± 0.053

0.909 ± 0.029

3.14 ± 0.044
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski