Lutibacter agarilyticus
Average proteome isoelectric point is 6.58
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3391 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A238W4H5|A0A238W4H5_9FLAO Por secretion system C-terminal sorting domain-containing protein OS=Lutibacter agarilyticus OX=1109740 GN=SAMN06265371_102409 PE=4 SV=1
MM1 pKa = 7.44 LKK3 pKa = 10.28 SIKK6 pKa = 10.15 LAILGCFFIIPLYY19 pKa = 10.65 AQTTIDD25 pKa = 3.68 SFDD28 pKa = 3.74 FEE30 pKa = 4.99 SGIEE34 pKa = 4.15 GWSQSAMDD42 pKa = 5.21 DD43 pKa = 3.37 FDD45 pKa = 6.84 LIRR48 pKa = 11.84 QSEE51 pKa = 4.45 GTPSNNTGPNTASSGNFYY69 pKa = 10.19 MFAEE73 pKa = 4.41 MSSPRR78 pKa = 11.84 EE79 pKa = 4.2 SGDD82 pKa = 3.43 SAIFTSPVIDD92 pKa = 4.02 LSGYY96 pKa = 6.51 MQPTFSFDD104 pKa = 3.19 YY105 pKa = 10.91 HH106 pKa = 6.27 MYY108 pKa = 9.96 SSNSISDD115 pKa = 3.55 VGTLALEE122 pKa = 4.13 INEE125 pKa = 4.93 NNTGWQSTSFSLTGSQNYY143 pKa = 9.61 DD144 pKa = 2.7 QDD146 pKa = 3.06 TWLPGLVDD154 pKa = 4.18 LLAYY158 pKa = 10.14 QGSSVQFRR166 pKa = 11.84 FVITRR171 pKa = 11.84 ANTWQGDD178 pKa = 3.64 IAFDD182 pKa = 4.2 NITIEE187 pKa = 4.12 GTALVAGVEE196 pKa = 4.35 IYY198 pKa = 10.73 VAGNGFEE205 pKa = 4.42 IPTGTTAISITNGTDD220 pKa = 3.5 FGGVNLASSSVAQTFTIEE238 pKa = 3.73 NWGLDD243 pKa = 3.51 DD244 pKa = 5.67 LVLNNSPYY252 pKa = 10.86 VSITGADD259 pKa = 3.23 AGHH262 pKa = 6.44 FSIDD266 pKa = 3.56 VASTTSPITSANSTTFQVTFSPLSLGTKK294 pKa = 8.1 TATIQISNNDD304 pKa = 3.53 TDD306 pKa = 3.49 EE307 pKa = 4.28 GLYY310 pKa = 10.59 SFNVQGTGVQNFFDD324 pKa = 3.94 SDD326 pKa = 3.43 GDD328 pKa = 4.21 GVFDD332 pKa = 5.57 HH333 pKa = 7.4 VDD335 pKa = 3.1 IDD337 pKa = 4.2 DD338 pKa = 5.31 DD339 pKa = 4.5 NDD341 pKa = 3.81 GVLDD345 pKa = 4.03 ALEE348 pKa = 4.46 EE349 pKa = 4.4 SACKK353 pKa = 10.16 SSSISVPANYY363 pKa = 10.13 KK364 pKa = 10.07 FLNEE368 pKa = 4.26 TFGTLAPGARR378 pKa = 11.84 TTINTSYY385 pKa = 11.18 NATTTYY391 pKa = 10.21 CYY393 pKa = 10.62 EE394 pKa = 4.09 EE395 pKa = 5.24 GIGTGSSCGSTWILDD410 pKa = 3.56 DD411 pKa = 3.85 GEE413 pKa = 4.6 YY414 pKa = 10.99 VVVSKK419 pKa = 9.77 ITGTDD424 pKa = 2.8 TADD427 pKa = 3.74 PDD429 pKa = 4.4 NIHH432 pKa = 7.32 GDD434 pKa = 3.93 LAWTNVLDD442 pKa = 3.83 HH443 pKa = 6.64 TADD446 pKa = 4.21 DD447 pKa = 4.63 GDD449 pKa = 3.99 TGGMAVFNAAWDD461 pKa = 3.79 PGVFYY466 pKa = 8.25 EE467 pKa = 4.39 TTITGILPNVPITYY481 pKa = 10.16 SFWVLNIMAEE491 pKa = 4.38 SKK493 pKa = 10.8 FPGSILPNVTVQFVDD508 pKa = 3.56 LFGNIITSIDD518 pKa = 3.07 TGEE521 pKa = 4.35 FGRR524 pKa = 11.84 CASGVNDD531 pKa = 3.71 NSCTANEE538 pKa = 4.18 WQNFTRR544 pKa = 11.84 DD545 pKa = 4.22 DD546 pKa = 3.62 IVLGNVDD553 pKa = 3.38 KK554 pKa = 11.12 FIVRR558 pKa = 11.84 FINNAPGGAGNDD570 pKa = 3.6 LALDD574 pKa = 5.07 DD575 pKa = 4.62 IVISQTLCDD584 pKa = 4.11 KK585 pKa = 11.22 DD586 pKa = 3.83 SDD588 pKa = 4.15 GVADD592 pKa = 5.14 VFDD595 pKa = 5.59 LDD597 pKa = 4.35 SDD599 pKa = 3.9 NDD601 pKa = 4.68 GIPDD605 pKa = 3.69 VVEE608 pKa = 4.31 SGLGIISLGTAKK620 pKa = 10.25 IPFPSGWVDD629 pKa = 3.29 ANGNGMHH636 pKa = 7.39 DD637 pKa = 3.43 AAEE640 pKa = 4.41 SNVILDD646 pKa = 4.02 SDD648 pKa = 4.21 SDD650 pKa = 3.88 GTPNYY655 pKa = 10.53 LDD657 pKa = 5.09 LDD659 pKa = 4.05 SDD661 pKa = 3.76 NDD663 pKa = 4.67 GIFDD667 pKa = 3.74 VDD669 pKa = 3.32 EE670 pKa = 4.83 SGAGNTANATFQNGDD685 pKa = 3.08 GDD687 pKa = 3.46 ISGNGVGQGPDD698 pKa = 2.89 TDD700 pKa = 4.1 YY701 pKa = 11.49 VRR703 pKa = 11.84 EE704 pKa = 3.97 TDD706 pKa = 3.81 YY707 pKa = 11.64 EE708 pKa = 4.38 SDD710 pKa = 3.24 GTLEE714 pKa = 4.05 YY715 pKa = 9.41 FTDD718 pKa = 5.91 GILDD722 pKa = 3.59 VYY724 pKa = 11.24 DD725 pKa = 3.96 YY726 pKa = 11.48 FNGSTFEE733 pKa = 3.92 TAYY736 pKa = 10.66 GNEE739 pKa = 4.02 NQGVGNTYY747 pKa = 10.73 YY748 pKa = 11.28 VLDD751 pKa = 4.37 SDD753 pKa = 5.12 ADD755 pKa = 4.41 GIPDD759 pKa = 3.75 YY760 pKa = 11.02 MDD762 pKa = 3.21 VTSDD766 pKa = 3.26 GVTFDD771 pKa = 4.11 ISHH774 pKa = 6.79 TLYY777 pKa = 11.34 ANLDD781 pKa = 3.58 ADD783 pKa = 3.9 NDD785 pKa = 4.91 GIIDD789 pKa = 3.91 DD790 pKa = 4.59 TNDD793 pKa = 3.32 SEE795 pKa = 5.17 GDD797 pKa = 3.93 GIVDD801 pKa = 5.67 LFDD804 pKa = 4.26 TDD806 pKa = 4.81 DD807 pKa = 3.77 SAFGSPRR814 pKa = 11.84 DD815 pKa = 3.87 LDD817 pKa = 3.63 RR818 pKa = 11.84 CLNLYY823 pKa = 10.45 FDD825 pKa = 4.47 GRR827 pKa = 11.84 NDD829 pKa = 3.55 YY830 pKa = 11.49 AEE832 pKa = 4.67 DD833 pKa = 3.64 SAVINAWGEE842 pKa = 3.83 ATMMCWIKK850 pKa = 10.05 MDD852 pKa = 4.37 PASAGIQHH860 pKa = 7.02 IMGQNEE866 pKa = 3.88 FNIQLNANKK875 pKa = 9.21 TISAHH880 pKa = 6.65 ASGNTLNYY888 pKa = 7.67 STPLNVNQWVHH899 pKa = 5.82 IATSYY904 pKa = 11.02 SSLNNEE910 pKa = 3.71 FKK912 pKa = 11.13 LFINGEE918 pKa = 4.14 EE919 pKa = 4.06 IAIEE923 pKa = 4.14 TGVSGALSADD933 pKa = 3.22 TSLFTIGKK941 pKa = 9.26 LPGVNSNFYY950 pKa = 10.05 HH951 pKa = 7.02 GYY953 pKa = 7.7 IDD955 pKa = 3.7 EE956 pKa = 4.12 VRR958 pKa = 11.84 VFNKK962 pKa = 10.31 ALSNNEE968 pKa = 3.76 LQKK971 pKa = 10.42 IVYY974 pKa = 9.33 QEE976 pKa = 3.85 IEE978 pKa = 4.15 NNGGIVRR985 pKa = 11.84 GSIIPMNVTNFIDD998 pKa = 4.02 EE999 pKa = 4.42 DD1000 pKa = 4.67 TVTPLDD1006 pKa = 3.24 WTYY1009 pKa = 10.56 LQRR1012 pKa = 11.84 YY1013 pKa = 8.32 FKK1015 pKa = 10.27 MDD1017 pKa = 3.17 VYY1019 pKa = 11.09 KK1020 pKa = 10.94 DD1021 pKa = 4.54 DD1022 pKa = 5.66 IIDD1025 pKa = 4.38 DD1026 pKa = 4.5 LSTPAVDD1033 pKa = 3.67 VGAGATIYY1041 pKa = 10.82 NMKK1044 pKa = 10.33 IIDD1047 pKa = 4.05 VQTAPLPFVTNQTGTLPTAVNHH1069 pKa = 6.77 TINGVNGNDD1078 pKa = 3.61 AIAYY1082 pKa = 7.47 DD1083 pKa = 3.63 WSIVNILHH1091 pKa = 7.27 DD1092 pKa = 3.74 AVYY1095 pKa = 10.0 YY1096 pKa = 11.02 NNYY1099 pKa = 9.02 QKK1101 pKa = 10.93 HH1102 pKa = 6.29 LGLIVNNLDD1111 pKa = 3.62 AGSNPIEE1118 pKa = 4.12 YY1119 pKa = 10.23 SIQNNSEE1126 pKa = 4.63 LNVSWYY1132 pKa = 10.84 LKK1134 pKa = 10.65 LDD1136 pKa = 3.44 GFIDD1140 pKa = 4.81 LEE1142 pKa = 4.65 GEE1144 pKa = 4.23 SQLVQGAEE1152 pKa = 4.12 SVLDD1156 pKa = 4.03 EE1157 pKa = 5.28 DD1158 pKa = 3.94 SVGYY1162 pKa = 10.08 IEE1164 pKa = 5.79 QDD1166 pKa = 3.28 QQGTQSSYY1174 pKa = 11.42 NYY1176 pKa = 9.54 NYY1178 pKa = 8.85 WSSSVSPIAAGAGTNNTSNTISEE1201 pKa = 4.37 VLFDD1205 pKa = 3.68 GTIANNPAAINFQPEE1220 pKa = 3.71 HH1221 pKa = 5.63 TAADD1225 pKa = 4.0 GAVTSPITLSSYY1237 pKa = 9.23 WLYY1240 pKa = 10.68 TFNGLDD1246 pKa = 3.25 NDD1248 pKa = 3.96 YY1249 pKa = 10.72 NSWVSIDD1256 pKa = 3.83 EE1257 pKa = 4.58 DD1258 pKa = 4.35 SSVLAGEE1265 pKa = 5.26 GYY1267 pKa = 8.83 TMKK1270 pKa = 10.02 GTSGVAAIIDD1280 pKa = 3.57 NQNYY1284 pKa = 7.78 VFKK1287 pKa = 10.64 GKK1289 pKa = 8.78 PHH1291 pKa = 6.58 NGDD1294 pKa = 2.75 ITLSIALNDD1303 pKa = 3.35 NRR1305 pKa = 11.84 LVGNPYY1311 pKa = 10.33 SSAMDD1316 pKa = 3.7 ANAFILDD1323 pKa = 4.34 NISDD1327 pKa = 3.96 SGGNAATNVFNGALYY1342 pKa = 10.11 FWDD1345 pKa = 3.84 HH1346 pKa = 6.49 FSGHH1350 pKa = 4.66 THH1352 pKa = 5.5 VLSEE1356 pKa = 4.23 YY1357 pKa = 8.87 VGGYY1361 pKa = 7.79 ATYY1364 pKa = 10.3 TLMGGAKK1371 pKa = 9.83 AYY1373 pKa = 10.69 ANDD1376 pKa = 4.07 EE1377 pKa = 4.43 RR1378 pKa = 11.84 IQNNGSSGVKK1388 pKa = 9.86 VPEE1391 pKa = 3.94 RR1392 pKa = 11.84 YY1393 pKa = 9.69 IPVNQGFFVNASLDD1407 pKa = 3.85 TVSNSISTSVTGGAVIFKK1425 pKa = 10.04 NSQRR1429 pKa = 11.84 VCVTEE1434 pKa = 3.78 NSGNSVFLKK1443 pKa = 10.13 SAHH1446 pKa = 6.21 SKK1448 pKa = 8.2 EE1449 pKa = 4.38 TTKK1452 pKa = 10.84 QLVVEE1457 pKa = 4.25 EE1458 pKa = 4.27 DD1459 pKa = 3.29 TRR1461 pKa = 11.84 SKK1463 pKa = 11.02 VRR1465 pKa = 11.84 LLFKK1469 pKa = 10.9 SPTGYY1474 pKa = 10.22 EE1475 pKa = 3.86 RR1476 pKa = 11.84 QLLVGADD1483 pKa = 3.66 ANASANFDD1491 pKa = 3.48 LGYY1494 pKa = 10.81 DD1495 pKa = 3.77 GFLIEE1500 pKa = 6.2 DD1501 pKa = 3.79 NVADD1505 pKa = 4.15 MFWAFSDD1512 pKa = 3.41 AKK1514 pKa = 10.47 FVIQAVDD1521 pKa = 3.45 TFKK1524 pKa = 11.11 LDD1526 pKa = 3.38 QEE1528 pKa = 4.52 LKK1530 pKa = 9.22 IGLRR1534 pKa = 11.84 IEE1536 pKa = 4.28 SAGIASISIDD1546 pKa = 3.33 TLEE1549 pKa = 4.91 NIDD1552 pKa = 3.97 EE1553 pKa = 4.4 GFEE1556 pKa = 4.71 LFIHH1560 pKa = 7.64 DD1561 pKa = 4.34 NLTGKK1566 pKa = 10.29 NYY1568 pKa = 10.58 DD1569 pKa = 3.54 IIKK1572 pKa = 10.48 NSAEE1576 pKa = 3.52 IHH1578 pKa = 6.13 LEE1580 pKa = 3.63 PGEE1583 pKa = 4.02 YY1584 pKa = 8.81 MDD1586 pKa = 5.48 RR1587 pKa = 11.84 FDD1589 pKa = 5.18 VRR1591 pKa = 11.84 FKK1593 pKa = 11.27 AEE1595 pKa = 3.74 KK1596 pKa = 8.69 TLSVEE1601 pKa = 4.71 NEE1603 pKa = 3.45 AALNGIHH1610 pKa = 6.3 VFVNNSSNEE1619 pKa = 3.92 LQIKK1623 pKa = 8.04 NTLNTEE1629 pKa = 3.65 IKK1631 pKa = 9.53 TICLYY1636 pKa = 11.06 NSLGQLQHH1644 pKa = 6.31 KK1645 pKa = 6.32 WQKK1648 pKa = 10.39 NLEE1651 pKa = 4.11 NAQISLPVNNKK1662 pKa = 8.75 VPGMYY1667 pKa = 10.42 LVQVKK1672 pKa = 9.49 TNTGLFNKK1680 pKa = 9.87 KK1681 pKa = 10.44 VIVNN1685 pKa = 4.18
Molecular weight: 182.46 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.692
IPC2_protein 3.821
IPC_protein 3.884
Toseland 3.643
ProMoST 4.037
Dawson 3.872
Bjellqvist 4.024
Wikipedia 3.808
Rodwell 3.694
Grimsley 3.554
Solomon 3.872
Lehninger 3.834
Nozaki 3.973
DTASelect 4.253
Thurlkill 3.694
EMBOSS 3.821
Sillero 3.999
Patrickios 1.265
IPC_peptide 3.872
IPC2_peptide 3.973
IPC2.peptide.svr19 3.878
Protein with the highest isoelectric point:
>tr|A0A238X0K8|A0A238X0K8_9FLAO PAS domain S-box-containing protein OS=Lutibacter agarilyticus OX=1109740 GN=SAMN06265371_104269 PE=4 SV=1
MM1 pKa = 7.18 EE2 pKa = 5.73 ALVKK6 pKa = 9.95 FIQDD10 pKa = 3.17 EE11 pKa = 4.5 FVTKK15 pKa = 10.64 KK16 pKa = 10.25 EE17 pKa = 4.12 FPQFQAGDD25 pKa = 4.11 TITVYY30 pKa = 11.01 YY31 pKa = 9.37 EE32 pKa = 3.62 IKK34 pKa = 10.3 EE35 pKa = 4.28 GEE37 pKa = 4.02 KK38 pKa = 9.87 TRR40 pKa = 11.84 TQFFRR45 pKa = 11.84 GVVIQRR51 pKa = 11.84 RR52 pKa = 11.84 GTGSSEE58 pKa = 3.8 TFTIRR63 pKa = 11.84 KK64 pKa = 7.76 MSGTVGVEE72 pKa = 3.69 RR73 pKa = 11.84 IFPVNLPAIQKK84 pKa = 9.91 IEE86 pKa = 4.02 VNKK89 pKa = 9.73 QGRR92 pKa = 11.84 VRR94 pKa = 11.84 RR95 pKa = 11.84 ARR97 pKa = 11.84 IFYY100 pKa = 10.34 FRR102 pKa = 11.84 GLTGKK107 pKa = 10.04 KK108 pKa = 10.2 ARR110 pKa = 11.84 IQEE113 pKa = 3.94 RR114 pKa = 11.84 RR115 pKa = 11.84 RR116 pKa = 3.43
Molecular weight: 13.59 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.313
IPC2_protein 9.794
IPC_protein 10.701
Toseland 10.994
ProMoST 11.023
Dawson 11.052
Bjellqvist 10.774
Wikipedia 11.286
Rodwell 11.257
Grimsley 11.082
Solomon 11.242
Lehninger 11.199
Nozaki 10.965
DTASelect 10.774
Thurlkill 10.979
EMBOSS 11.403
Sillero 10.994
Patrickios 10.994
IPC_peptide 11.242
IPC2_peptide 9.589
IPC2.peptide.svr19 8.733
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3391
0
3391
1235752
26
3453
364.4
41.12
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.136 ± 0.038
0.759 ± 0.015
5.311 ± 0.036
6.735 ± 0.038
5.26 ± 0.033
6.379 ± 0.041
1.704 ± 0.017
8.298 ± 0.041
8.075 ± 0.051
9.07 ± 0.045
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.11 ± 0.02
6.648 ± 0.041
3.35 ± 0.026
3.143 ± 0.02
3.036 ± 0.027
6.507 ± 0.034
5.911 ± 0.045
6.286 ± 0.035
1.129 ± 0.016
4.151 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here