Gordonia phage Bibwit

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Vividuovirus; unclassified Vividuovirus

Average proteome isoelectric point is 5.99

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 83 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3G3M7X0|A0A3G3M7X0_9CAUD Uncharacterized protein OS=Gordonia phage Bibwit OX=2483666 GN=59 PE=4 SV=1
MM1 pKa = 6.5KK2 pKa = 9.84TRR4 pKa = 11.84YY5 pKa = 9.27KK6 pKa = 10.74VLAGVGAFVVTLGIIGAVTDD26 pKa = 4.24DD27 pKa = 4.05GEE29 pKa = 4.37ATVRR33 pKa = 11.84GNDD36 pKa = 3.24VQTAAVLPSTTAPAHH51 pKa = 4.81TTVEE55 pKa = 4.06LDD57 pKa = 3.46EE58 pKa = 4.81PATIEE63 pKa = 4.16TTTTTEE69 pKa = 3.83APTVEE74 pKa = 4.53TVDD77 pKa = 3.84TPSVTVSQTQALEE90 pKa = 3.98SAEE93 pKa = 4.1QYY95 pKa = 10.15LAYY98 pKa = 10.68VGGFSRR104 pKa = 11.84AEE106 pKa = 4.43LIDD109 pKa = 3.43QLVYY113 pKa = 11.18EE114 pKa = 4.8DD115 pKa = 5.13FSRR118 pKa = 11.84ADD120 pKa = 3.58AEE122 pKa = 4.24WAVDD126 pKa = 3.5NVTVDD131 pKa = 2.89WFEE134 pKa = 3.93QAEE137 pKa = 4.45KK138 pKa = 10.74SAADD142 pKa = 3.65YY143 pKa = 11.2IEE145 pKa = 4.06FVGGFSYY152 pKa = 11.04QEE154 pKa = 4.06LVDD157 pKa = 3.64QLVYY161 pKa = 10.86EE162 pKa = 4.41QFTQEE167 pKa = 4.06QAEE170 pKa = 4.37HH171 pKa = 6.2GAKK174 pKa = 10.26SVDD177 pKa = 3.62LTPP180 pKa = 4.65

Molecular weight: 19.44 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3G3M804|A0A3G3M804_9CAUD Portal protein OS=Gordonia phage Bibwit OX=2483666 GN=12 PE=4 SV=1
MM1 pKa = 7.26GWLLFGAVFIPVRR14 pKa = 11.84IAKK17 pKa = 9.9IIEE20 pKa = 4.48DD21 pKa = 3.61ALSPRR26 pKa = 11.84RR27 pKa = 11.84RR28 pKa = 11.84RR29 pKa = 11.84EE30 pKa = 3.9LIQQQTRR37 pKa = 11.84DD38 pKa = 3.68EE39 pKa = 4.24VAAAAEE45 pKa = 4.0RR46 pKa = 11.84SEE48 pKa = 4.04QYY50 pKa = 10.41RR51 pKa = 11.84RR52 pKa = 11.84TRR54 pKa = 11.84YY55 pKa = 10.13GLL57 pKa = 3.49

Molecular weight: 6.7 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

83

0

83

18431

37

1607

222.1

24.17

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.947 ± 0.404

0.852 ± 0.126

7.732 ± 0.418

5.561 ± 0.344

2.463 ± 0.152

8.529 ± 0.474

2.317 ± 0.202

4.547 ± 0.267

2.48 ± 0.177

7.515 ± 0.245

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.1 ± 0.105

2.68 ± 0.156

6.478 ± 0.209

3.776 ± 0.2

7.618 ± 0.442

4.731 ± 0.183

6.625 ± 0.279

7.791 ± 0.246

2.078 ± 0.104

2.181 ± 0.135
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski