Amino acid dipepetide frequency for Gordonia phage Bibwit

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
16.658AlaAla: 16.658 ± 1.169
0.543AlaCys: 0.543 ± 0.204
8.41AlaAsp: 8.41 ± 0.762
9.17AlaGlu: 9.17 ± 0.985
2.984AlaPhe: 2.984 ± 0.538
11.123AlaGly: 11.123 ± 1.083
2.116AlaHis: 2.116 ± 0.342
6.511AlaIle: 6.511 ± 0.89
3.201AlaLys: 3.201 ± 0.468
9.17AlaLeu: 9.17 ± 0.905
2.279AlaMet: 2.279 ± 0.367
2.876AlaAsn: 2.876 ± 0.478
6.674AlaPro: 6.674 ± 0.777
4.286AlaGln: 4.286 ± 0.411
8.519AlaArg: 8.519 ± 0.857
5.155AlaSer: 5.155 ± 0.559
7.162AlaThr: 7.162 ± 0.746
8.03AlaVal: 8.03 ± 0.616
2.279AlaTrp: 2.279 ± 0.317
2.333AlaTyr: 2.333 ± 0.364
0.0AlaXaa: 0.0 ± 0.0
Cys
1.031CysAla: 1.031 ± 0.256
0.0CysCys: 0.0 ± 0.0
0.651CysAsp: 0.651 ± 0.187
0.38CysGlu: 0.38 ± 0.132
0.109CysPhe: 0.109 ± 0.076
1.194CysGly: 1.194 ± 0.336
0.597CysHis: 0.597 ± 0.19
0.054CysIle: 0.054 ± 0.066
0.217CysLys: 0.217 ± 0.104
0.38CysLeu: 0.38 ± 0.141
0.109CysMet: 0.109 ± 0.082
0.109CysAsn: 0.109 ± 0.062
0.868CysPro: 0.868 ± 0.219
0.38CysGln: 0.38 ± 0.136
0.543CysArg: 0.543 ± 0.197
0.434CysSer: 0.434 ± 0.176
0.814CysThr: 0.814 ± 0.221
0.488CysVal: 0.488 ± 0.173
0.109CysTrp: 0.109 ± 0.07
0.054CysTyr: 0.054 ± 0.06
0.0CysXaa: 0.0 ± 0.0
Asp
8.627AspAla: 8.627 ± 0.749
0.543AspCys: 0.543 ± 0.256
8.03AspAsp: 8.03 ± 1.053
6.131AspGlu: 6.131 ± 0.863
1.248AspPhe: 1.248 ± 0.284
7.108AspGly: 7.108 ± 0.704
1.953AspHis: 1.953 ± 0.364
1.682AspIle: 1.682 ± 0.371
1.465AspLys: 1.465 ± 0.23
5.534AspLeu: 5.534 ± 0.573
1.574AspMet: 1.574 ± 0.28
2.225AspAsn: 2.225 ± 0.382
7.705AspPro: 7.705 ± 0.838
2.496AspGln: 2.496 ± 0.449
5.209AspArg: 5.209 ± 0.554
2.17AspSer: 2.17 ± 0.298
3.744AspThr: 3.744 ± 0.531
6.24AspVal: 6.24 ± 0.626
1.899AspTrp: 1.899 ± 0.357
1.736AspTyr: 1.736 ± 0.37
0.0AspXaa: 0.0 ± 0.0
Glu
5.48GluAla: 5.48 ± 0.502
0.705GluCys: 0.705 ± 0.208
2.984GluAsp: 2.984 ± 0.505
1.411GluGlu: 1.411 ± 0.289
2.279GluPhe: 2.279 ± 0.383
4.015GluGly: 4.015 ± 0.459
2.387GluHis: 2.387 ± 0.402
2.279GluIle: 2.279 ± 0.308
1.194GluLys: 1.194 ± 0.331
4.721GluLeu: 4.721 ± 0.763
1.139GluMet: 1.139 ± 0.265
1.736GluAsn: 1.736 ± 0.232
3.418GluPro: 3.418 ± 0.525
4.015GluGln: 4.015 ± 0.484
4.504GluArg: 4.504 ± 0.687
2.713GluSer: 2.713 ± 0.44
2.767GluThr: 2.767 ± 0.508
4.612GluVal: 4.612 ± 0.518
1.574GluTrp: 1.574 ± 0.36
1.682GluTyr: 1.682 ± 0.32
0.0GluXaa: 0.0 ± 0.0
Phe
3.147PheAla: 3.147 ± 0.373
0.326PheCys: 0.326 ± 0.153
2.713PheAsp: 2.713 ± 0.443
1.519PheGlu: 1.519 ± 0.371
0.76PhePhe: 0.76 ± 0.249
2.659PheGly: 2.659 ± 0.298
0.38PheHis: 0.38 ± 0.137
1.356PheIle: 1.356 ± 0.32
0.814PheLys: 0.814 ± 0.179
1.411PheLeu: 1.411 ± 0.3
0.597PheMet: 0.597 ± 0.128
0.651PheAsn: 0.651 ± 0.216
1.139PhePro: 1.139 ± 0.239
0.488PheGln: 0.488 ± 0.172
1.139PheArg: 1.139 ± 0.202
0.977PheSer: 0.977 ± 0.26
1.736PheThr: 1.736 ± 0.279
2.387PheVal: 2.387 ± 0.268
0.326PheTrp: 0.326 ± 0.128
0.109PheTyr: 0.109 ± 0.069
0.0PheXaa: 0.0 ± 0.0
Gly
9.007GlyAla: 9.007 ± 0.93
0.651GlyCys: 0.651 ± 0.217
6.999GlyAsp: 6.999 ± 0.725
4.232GlyGlu: 4.232 ± 0.528
2.062GlyPhe: 2.062 ± 0.345
7.813GlyGly: 7.813 ± 1.095
2.387GlyHis: 2.387 ± 0.387
4.015GlyIle: 4.015 ± 0.468
3.364GlyLys: 3.364 ± 0.454
5.589GlyLeu: 5.589 ± 0.72
1.953GlyMet: 1.953 ± 0.301
2.604GlyAsn: 2.604 ± 0.478
3.798GlyPro: 3.798 ± 0.518
3.093GlyGln: 3.093 ± 0.474
6.403GlyArg: 6.403 ± 0.678
5.155GlySer: 5.155 ± 0.597
5.426GlyThr: 5.426 ± 0.728
7.271GlyVal: 7.271 ± 0.598
1.302GlyTrp: 1.302 ± 0.284
2.17GlyTyr: 2.17 ± 0.407
0.0GlyXaa: 0.0 ± 0.0
His
1.845HisAla: 1.845 ± 0.401
0.163HisCys: 0.163 ± 0.095
2.062HisAsp: 2.062 ± 0.348
1.736HisGlu: 1.736 ± 0.418
0.488HisPhe: 0.488 ± 0.159
1.302HisGly: 1.302 ± 0.234
0.434HisHis: 0.434 ± 0.158
0.814HisIle: 0.814 ± 0.233
0.434HisLys: 0.434 ± 0.154
1.736HisLeu: 1.736 ± 0.305
0.543HisMet: 0.543 ± 0.166
0.488HisAsn: 0.488 ± 0.152
1.411HisPro: 1.411 ± 0.296
1.248HisGln: 1.248 ± 0.226
2.225HisArg: 2.225 ± 0.38
0.977HisSer: 0.977 ± 0.219
2.333HisThr: 2.333 ± 0.467
2.008HisVal: 2.008 ± 0.33
0.488HisTrp: 0.488 ± 0.164
0.434HisTyr: 0.434 ± 0.147
0.0HisXaa: 0.0 ± 0.0
Ile
5.914IleAla: 5.914 ± 0.472
0.109IleCys: 0.109 ± 0.066
4.286IleAsp: 4.286 ± 0.429
4.015IleGlu: 4.015 ± 0.397
0.434IlePhe: 0.434 ± 0.199
5.1IleGly: 5.1 ± 1.093
0.651IleHis: 0.651 ± 0.162
2.713IleIle: 2.713 ± 0.46
0.868IleLys: 0.868 ± 0.286
2.333IleLeu: 2.333 ± 0.36
0.76IleMet: 0.76 ± 0.217
0.922IleAsn: 0.922 ± 0.262
2.659IlePro: 2.659 ± 0.282
1.411IleGln: 1.411 ± 0.351
2.93IleArg: 2.93 ± 0.357
1.736IleSer: 1.736 ± 0.358
3.364IleThr: 3.364 ± 0.424
3.527IleVal: 3.527 ± 0.372
0.76IleTrp: 0.76 ± 0.199
0.977IleTyr: 0.977 ± 0.197
0.0IleXaa: 0.0 ± 0.0
Lys
2.604LysAla: 2.604 ± 0.392
0.217LysCys: 0.217 ± 0.149
0.868LysAsp: 0.868 ± 0.259
0.651LysGlu: 0.651 ± 0.179
0.977LysPhe: 0.977 ± 0.25
1.574LysGly: 1.574 ± 0.328
0.597LysHis: 0.597 ± 0.178
1.085LysIle: 1.085 ± 0.251
0.597LysLys: 0.597 ± 0.176
2.387LysLeu: 2.387 ± 0.346
0.38LysMet: 0.38 ± 0.145
0.977LysAsn: 0.977 ± 0.234
1.791LysPro: 1.791 ± 0.29
0.922LysGln: 0.922 ± 0.171
2.225LysArg: 2.225 ± 0.376
1.682LysSer: 1.682 ± 0.379
1.899LysThr: 1.899 ± 0.28
2.225LysVal: 2.225 ± 0.304
0.326LysTrp: 0.326 ± 0.125
0.814LysTyr: 0.814 ± 0.227
0.0LysXaa: 0.0 ± 0.0
Leu
9.116LeuAla: 9.116 ± 0.737
0.651LeuCys: 0.651 ± 0.183
5.697LeuAsp: 5.697 ± 0.641
2.387LeuGlu: 2.387 ± 0.39
2.062LeuPhe: 2.062 ± 0.365
6.403LeuGly: 6.403 ± 1.193
2.008LeuHis: 2.008 ± 0.387
3.798LeuIle: 3.798 ± 0.449
1.139LeuLys: 1.139 ± 0.27
5.48LeuLeu: 5.48 ± 0.541
1.248LeuMet: 1.248 ± 0.245
2.387LeuAsn: 2.387 ± 0.326
4.775LeuPro: 4.775 ± 0.464
3.256LeuGln: 3.256 ± 0.414
6.294LeuArg: 6.294 ± 0.533
3.635LeuSer: 3.635 ± 0.398
5.969LeuThr: 5.969 ± 0.643
5.697LeuVal: 5.697 ± 0.536
1.899LeuTrp: 1.899 ± 0.289
1.248LeuTyr: 1.248 ± 0.321
0.0LeuXaa: 0.0 ± 0.0
Met
1.899MetAla: 1.899 ± 0.358
0.163MetCys: 0.163 ± 0.081
0.868MetAsp: 0.868 ± 0.214
0.705MetGlu: 0.705 ± 0.235
0.38MetPhe: 0.38 ± 0.188
1.248MetGly: 1.248 ± 0.263
0.271MetHis: 0.271 ± 0.108
0.651MetIle: 0.651 ± 0.153
0.651MetLys: 0.651 ± 0.151
1.736MetLeu: 1.736 ± 0.355
0.434MetMet: 0.434 ± 0.147
0.651MetAsn: 0.651 ± 0.214
2.17MetPro: 2.17 ± 0.373
0.868MetGln: 0.868 ± 0.318
1.736MetArg: 1.736 ± 0.287
1.411MetSer: 1.411 ± 0.245
2.984MetThr: 2.984 ± 0.39
1.682MetVal: 1.682 ± 0.369
0.38MetTrp: 0.38 ± 0.171
0.109MetTyr: 0.109 ± 0.083
0.0MetXaa: 0.0 ± 0.0
Asn
3.744AsnAla: 3.744 ± 0.542
0.271AsnCys: 0.271 ± 0.11
2.008AsnAsp: 2.008 ± 0.34
1.411AsnGlu: 1.411 ± 0.319
0.597AsnPhe: 0.597 ± 0.164
2.876AsnGly: 2.876 ± 0.369
0.488AsnHis: 0.488 ± 0.122
0.814AsnIle: 0.814 ± 0.194
0.38AsnLys: 0.38 ± 0.168
2.279AsnLeu: 2.279 ± 0.543
0.651AsnMet: 0.651 ± 0.15
0.651AsnAsn: 0.651 ± 0.184
2.496AsnPro: 2.496 ± 0.404
0.76AsnGln: 0.76 ± 0.255
1.682AsnArg: 1.682 ± 0.295
0.977AsnSer: 0.977 ± 0.245
1.519AsnThr: 1.519 ± 0.262
1.736AsnVal: 1.736 ± 0.295
0.76AsnTrp: 0.76 ± 0.212
0.705AsnTyr: 0.705 ± 0.203
0.0AsnXaa: 0.0 ± 0.0
Pro
8.681ProAla: 8.681 ± 0.814
0.217ProCys: 0.217 ± 0.151
5.914ProAsp: 5.914 ± 0.792
3.256ProGlu: 3.256 ± 0.421
1.791ProPhe: 1.791 ± 0.285
4.992ProGly: 4.992 ± 0.499
1.139ProHis: 1.139 ± 0.307
3.418ProIle: 3.418 ± 0.502
1.465ProLys: 1.465 ± 0.282
4.612ProLeu: 4.612 ± 0.409
1.465ProMet: 1.465 ± 0.235
2.279ProAsn: 2.279 ± 0.409
4.558ProPro: 4.558 ± 0.645
1.899ProGln: 1.899 ± 0.307
3.852ProArg: 3.852 ± 0.421
3.69ProSer: 3.69 ± 0.396
4.232ProThr: 4.232 ± 0.519
4.829ProVal: 4.829 ± 0.645
1.085ProTrp: 1.085 ± 0.269
1.356ProTyr: 1.356 ± 0.233
0.0ProXaa: 0.0 ± 0.0
Gln
5.209GlnAla: 5.209 ± 0.47
0.326GlnCys: 0.326 ± 0.135
1.139GlnAsp: 1.139 ± 0.263
1.031GlnGlu: 1.031 ± 0.225
1.248GlnPhe: 1.248 ± 0.224
2.116GlnGly: 2.116 ± 0.342
0.922GlnHis: 0.922 ± 0.261
1.899GlnIle: 1.899 ± 0.404
0.814GlnLys: 0.814 ± 0.176
3.581GlnLeu: 3.581 ± 0.428
1.194GlnMet: 1.194 ± 0.256
0.868GlnAsn: 0.868 ± 0.215
2.713GlnPro: 2.713 ± 0.403
2.659GlnGln: 2.659 ± 0.465
4.015GlnArg: 4.015 ± 0.482
1.519GlnSer: 1.519 ± 0.289
2.062GlnThr: 2.062 ± 0.346
2.713GlnVal: 2.713 ± 0.34
0.977GlnTrp: 0.977 ± 0.196
0.76GlnTyr: 0.76 ± 0.146
0.0GlnXaa: 0.0 ± 0.0
Arg
9.17ArgAla: 9.17 ± 0.838
0.597ArgCys: 0.597 ± 0.154
5.317ArgAsp: 5.317 ± 0.672
3.635ArgGlu: 3.635 ± 0.486
1.682ArgPhe: 1.682 ± 0.326
4.721ArgGly: 4.721 ± 0.623
1.682ArgHis: 1.682 ± 0.399
3.744ArgIle: 3.744 ± 0.413
2.496ArgLys: 2.496 ± 0.429
5.48ArgLeu: 5.48 ± 0.584
2.225ArgMet: 2.225 ± 0.297
2.17ArgAsn: 2.17 ± 0.326
4.178ArgPro: 4.178 ± 0.529
2.496ArgGln: 2.496 ± 0.365
8.681ArgArg: 8.681 ± 1.253
3.581ArgSer: 3.581 ± 0.516
4.232ArgThr: 4.232 ± 0.516
5.86ArgVal: 5.86 ± 0.794
2.008ArgTrp: 2.008 ± 0.37
2.225ArgTyr: 2.225 ± 0.35
0.0ArgXaa: 0.0 ± 0.0
Ser
4.883SerAla: 4.883 ± 0.539
0.651SerCys: 0.651 ± 0.249
3.798SerAsp: 3.798 ± 0.515
2.604SerGlu: 2.604 ± 0.454
1.085SerPhe: 1.085 ± 0.236
5.426SerGly: 5.426 ± 0.759
0.76SerHis: 0.76 ± 0.223
2.225SerIle: 2.225 ± 0.477
1.194SerLys: 1.194 ± 0.266
3.093SerLeu: 3.093 ± 0.4
0.868SerMet: 0.868 ± 0.256
1.031SerAsn: 1.031 ± 0.257
3.039SerPro: 3.039 ± 0.407
1.302SerGln: 1.302 ± 0.314
3.527SerArg: 3.527 ± 0.514
2.442SerSer: 2.442 ± 0.488
4.124SerThr: 4.124 ± 0.475
3.256SerVal: 3.256 ± 0.334
0.868SerTrp: 0.868 ± 0.196
1.139SerTyr: 1.139 ± 0.231
0.0SerXaa: 0.0 ± 0.0
Thr
8.844ThrAla: 8.844 ± 0.905
1.031ThrCys: 1.031 ± 0.308
5.697ThrAsp: 5.697 ± 0.566
3.364ThrGlu: 3.364 ± 0.435
1.411ThrPhe: 1.411 ± 0.257
5.534ThrGly: 5.534 ± 0.657
1.465ThrHis: 1.465 ± 0.33
2.984ThrIle: 2.984 ± 0.446
1.574ThrLys: 1.574 ± 0.259
5.372ThrLeu: 5.372 ± 0.584
0.922ThrMet: 0.922 ± 0.214
1.194ThrAsn: 1.194 ± 0.231
5.426ThrPro: 5.426 ± 0.537
1.628ThrGln: 1.628 ± 0.246
4.124ThrArg: 4.124 ± 0.461
3.31ThrSer: 3.31 ± 0.401
4.395ThrThr: 4.395 ± 0.596
5.643ThrVal: 5.643 ± 0.671
1.031ThrTrp: 1.031 ± 0.249
1.302ThrTyr: 1.302 ± 0.261
0.0ThrXaa: 0.0 ± 0.0
Val
8.464ValAla: 8.464 ± 0.754
0.868ValCys: 0.868 ± 0.299
7.216ValAsp: 7.216 ± 0.631
5.046ValGlu: 5.046 ± 0.532
1.682ValPhe: 1.682 ± 0.271
7.054ValGly: 7.054 ± 0.551
1.899ValHis: 1.899 ± 0.319
3.418ValIle: 3.418 ± 0.423
1.791ValLys: 1.791 ± 0.232
6.186ValLeu: 6.186 ± 0.559
1.356ValMet: 1.356 ± 0.24
2.279ValAsn: 2.279 ± 0.365
4.015ValPro: 4.015 ± 0.522
2.93ValGln: 2.93 ± 0.353
5.046ValArg: 5.046 ± 0.584
3.364ValSer: 3.364 ± 0.4
5.426ValThr: 5.426 ± 0.561
5.969ValVal: 5.969 ± 0.663
1.899ValTrp: 1.899 ± 0.321
2.008ValTyr: 2.008 ± 0.402
0.0ValXaa: 0.0 ± 0.0
Trp
2.279TrpAla: 2.279 ± 0.31
0.271TrpCys: 0.271 ± 0.116
0.922TrpAsp: 0.922 ± 0.202
1.085TrpGlu: 1.085 ± 0.233
0.705TrpPhe: 0.705 ± 0.183
1.085TrpGly: 1.085 ± 0.252
0.488TrpHis: 0.488 ± 0.222
1.248TrpIle: 1.248 ± 0.304
0.705TrpLys: 0.705 ± 0.186
2.225TrpLeu: 2.225 ± 0.327
0.76TrpMet: 0.76 ± 0.181
0.488TrpAsn: 0.488 ± 0.157
1.139TrpPro: 1.139 ± 0.221
1.031TrpGln: 1.031 ± 0.207
1.465TrpArg: 1.465 ± 0.314
1.465TrpSer: 1.465 ± 0.266
0.814TrpThr: 0.814 ± 0.222
1.845TrpVal: 1.845 ± 0.282
0.38TrpTrp: 0.38 ± 0.202
0.38TrpTyr: 0.38 ± 0.125
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.876TyrAla: 2.876 ± 0.523
0.217TyrCys: 0.217 ± 0.097
1.736TyrAsp: 1.736 ± 0.325
1.845TyrGlu: 1.845 ± 0.379
0.651TyrPhe: 0.651 ± 0.187
1.953TyrGly: 1.953 ± 0.247
0.543TyrHis: 0.543 ± 0.16
0.76TyrIle: 0.76 ± 0.187
0.434TyrLys: 0.434 ± 0.16
1.845TyrLeu: 1.845 ± 0.378
0.488TyrMet: 0.488 ± 0.149
0.217TyrAsn: 0.217 ± 0.099
0.814TyrPro: 0.814 ± 0.213
0.597TyrGln: 0.597 ± 0.149
2.062TyrArg: 2.062 ± 0.425
0.922TyrSer: 0.922 ± 0.205
1.248TyrThr: 1.248 ± 0.281
1.899TyrVal: 1.899 ± 0.344
0.434TyrTrp: 0.434 ± 0.138
0.271TyrTyr: 0.271 ± 0.122
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 83 proteins (18431 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski