Synechococcus sp. PCC 7502
Average proteome isoelectric point is 6.38
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3310 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|K9SZE6|K9SZE6_9SYNE tRNA (guanine-N(7)-)-methyltransferase OS=Synechococcus sp. PCC 7502 OX=1173263 GN=trmB PE=3 SV=1
MM1 pKa = 7.67 EE2 pKa = 5.81 KK3 pKa = 10.47 YY4 pKa = 9.5 ICKK7 pKa = 8.33 TCGYY11 pKa = 9.95 VYY13 pKa = 10.57 DD14 pKa = 4.83 PALGDD19 pKa = 3.8 PDD21 pKa = 4.77 SGIEE25 pKa = 3.77 PGIPFGALPDD35 pKa = 4.05 DD36 pKa = 4.37 WVCPKK41 pKa = 10.65 CGTPKK46 pKa = 10.74 ADD48 pKa = 5.03 FEE50 pKa = 4.86 PEE52 pKa = 3.95 DD53 pKa = 4.16 TPATDD58 pKa = 3.47 VPLMEE63 pKa = 4.82 PVV65 pKa = 2.95
Molecular weight: 7.0 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.794
IPC2_protein 3.795
IPC_protein 3.719
Toseland 3.516
ProMoST 3.91
Dawson 3.719
Bjellqvist 3.884
Wikipedia 3.681
Rodwell 3.554
Grimsley 3.427
Solomon 3.694
Lehninger 3.643
Nozaki 3.859
DTASelect 4.062
Thurlkill 3.592
EMBOSS 3.694
Sillero 3.846
Patrickios 0.401
IPC_peptide 3.694
IPC2_peptide 3.808
IPC2.peptide.svr19 3.754
Protein with the highest isoelectric point:
>tr|K9SW58|K9SW58_9SYNE Cytosine deaminase-like metal-dependent hydrolase OS=Synechococcus sp. PCC 7502 OX=1173263 GN=Syn7502_02415 PE=4 SV=1
MM1 pKa = 6.61 TQRR4 pKa = 11.84 TLGGTARR11 pKa = 11.84 KK12 pKa = 9.37 KK13 pKa = 10.41 RR14 pKa = 11.84 RR15 pKa = 11.84 TSGFRR20 pKa = 11.84 ARR22 pKa = 11.84 MKK24 pKa = 10.12 SHH26 pKa = 7.23 TGRR29 pKa = 11.84 AVIKK33 pKa = 10.14 ARR35 pKa = 11.84 RR36 pKa = 11.84 SRR38 pKa = 11.84 GRR40 pKa = 11.84 LRR42 pKa = 11.84 LTTVV46 pKa = 2.73
Molecular weight: 5.27 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.286
IPC_protein 12.881
Toseland 13.042
ProMoST 13.539
Dawson 13.042
Bjellqvist 13.042
Wikipedia 13.525
Rodwell 12.676
Grimsley 13.086
Solomon 13.539
Lehninger 13.437
Nozaki 13.042
DTASelect 13.042
Thurlkill 13.042
EMBOSS 13.539
Sillero 13.042
Patrickios 12.398
IPC_peptide 13.539
IPC2_peptide 12.53
IPC2.peptide.svr19 9.215
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3310
0
3310
995375
29
2814
300.7
33.45
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.051 ± 0.049
0.948 ± 0.014
5.089 ± 0.035
5.858 ± 0.043
3.984 ± 0.031
6.566 ± 0.043
1.831 ± 0.025
7.721 ± 0.033
5.122 ± 0.039
10.957 ± 0.053
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.921 ± 0.019
4.553 ± 0.042
4.438 ± 0.031
4.971 ± 0.043
4.716 ± 0.036
6.73 ± 0.039
5.674 ± 0.044
6.488 ± 0.036
1.338 ± 0.017
3.044 ± 0.027
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here