Amino acid dipepetide frequency for Synechococcus sp. PCC 7502

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.432AlaAla: 6.432 ± 0.101
0.812AlaCys: 0.812 ± 0.031
3.976AlaAsp: 3.976 ± 0.058
5.102AlaGlu: 5.102 ± 0.086
2.828AlaPhe: 2.828 ± 0.061
5.269AlaGly: 5.269 ± 0.1
1.302AlaHis: 1.302 ± 0.042
8.578AlaIle: 8.578 ± 0.105
4.543AlaLys: 4.543 ± 0.07
8.293AlaLeu: 8.293 ± 0.101
1.735AlaMet: 1.735 ± 0.039
3.697AlaAsn: 3.697 ± 0.084
2.839AlaPro: 2.839 ± 0.056
3.966AlaGln: 3.966 ± 0.072
3.177AlaArg: 3.177 ± 0.064
4.757AlaSer: 4.757 ± 0.072
4.44AlaThr: 4.44 ± 0.077
5.388AlaVal: 5.388 ± 0.077
1.037AlaTrp: 1.037 ± 0.033
2.341AlaTyr: 2.341 ± 0.057
0.0AlaXaa: 0.0 ± 0.0
Cys
0.617CysAla: 0.617 ± 0.027
0.139CysCys: 0.139 ± 0.013
0.713CysAsp: 0.713 ± 0.028
0.489CysGlu: 0.489 ± 0.021
0.34CysPhe: 0.34 ± 0.02
0.792CysGly: 0.792 ± 0.03
0.295CysHis: 0.295 ± 0.016
0.599CysIle: 0.599 ± 0.026
0.379CysLys: 0.379 ± 0.021
1.103CysLeu: 1.103 ± 0.035
0.166CysMet: 0.166 ± 0.013
0.354CysAsn: 0.354 ± 0.018
0.485CysPro: 0.485 ± 0.025
0.542CysGln: 0.542 ± 0.027
0.445CysArg: 0.445 ± 0.025
0.583CysSer: 0.583 ± 0.03
0.457CysThr: 0.457 ± 0.023
0.564CysVal: 0.564 ± 0.026
0.113CysTrp: 0.113 ± 0.011
0.304CysTyr: 0.304 ± 0.016
0.0CysXaa: 0.0 ± 0.0
Asp
3.439AspAla: 3.439 ± 0.064
0.482AspCys: 0.482 ± 0.023
2.14AspAsp: 2.14 ± 0.057
2.496AspGlu: 2.496 ± 0.054
2.372AspPhe: 2.372 ± 0.058
3.219AspGly: 3.219 ± 0.063
1.082AspHis: 1.082 ± 0.034
3.775AspIle: 3.775 ± 0.061
2.109AspLys: 2.109 ± 0.055
6.393AspLeu: 6.393 ± 0.092
0.893AspMet: 0.893 ± 0.028
1.816AspAsn: 1.816 ± 0.05
2.529AspPro: 2.529 ± 0.052
2.926AspGln: 2.926 ± 0.063
4.278AspArg: 4.278 ± 0.07
2.826AspSer: 2.826 ± 0.055
2.458AspThr: 2.458 ± 0.066
2.935AspVal: 2.935 ± 0.061
0.789AspTrp: 0.789 ± 0.029
1.937AspTyr: 1.937 ± 0.053
0.0AspXaa: 0.0 ± 0.0
Glu
5.03GluAla: 5.03 ± 0.087
0.437GluCys: 0.437 ± 0.02
2.531GluAsp: 2.531 ± 0.058
3.172GluGlu: 3.172 ± 0.061
2.557GluPhe: 2.557 ± 0.052
2.89GluGly: 2.89 ± 0.057
0.988GluHis: 0.988 ± 0.037
5.374GluIle: 5.374 ± 0.081
2.972GluLys: 2.972 ± 0.061
7.03GluLeu: 7.03 ± 0.103
1.254GluMet: 1.254 ± 0.037
2.464GluAsn: 2.464 ± 0.053
2.255GluPro: 2.255 ± 0.059
3.037GluGln: 3.037 ± 0.057
2.873GluArg: 2.873 ± 0.06
3.694GluSer: 3.694 ± 0.061
3.199GluThr: 3.199 ± 0.058
4.314GluVal: 4.314 ± 0.074
0.689GluTrp: 0.689 ± 0.027
1.818GluTyr: 1.818 ± 0.051
0.0GluXaa: 0.0 ± 0.0
Phe
3.119PheAla: 3.119 ± 0.052
0.503PheCys: 0.503 ± 0.022
2.319PheAsp: 2.319 ± 0.049
2.122PheGlu: 2.122 ± 0.051
1.631PhePhe: 1.631 ± 0.046
2.943PheGly: 2.943 ± 0.06
0.678PheHis: 0.678 ± 0.023
2.732PheIle: 2.732 ± 0.064
1.873PheLys: 1.873 ± 0.046
4.114PheLeu: 4.114 ± 0.08
0.783PheMet: 0.783 ± 0.027
1.954PheAsn: 1.954 ± 0.057
1.797PhePro: 1.797 ± 0.042
1.662PheGln: 1.662 ± 0.04
1.691PheArg: 1.691 ± 0.039
2.969PheSer: 2.969 ± 0.059
2.57PheThr: 2.57 ± 0.054
2.364PheVal: 2.364 ± 0.059
0.66PheTrp: 0.66 ± 0.026
1.354PheTyr: 1.354 ± 0.037
0.0PheXaa: 0.0 ± 0.0
Gly
5.098GlyAla: 5.098 ± 0.098
0.736GlyCys: 0.736 ± 0.029
3.458GlyAsp: 3.458 ± 0.066
3.585GlyGlu: 3.585 ± 0.067
3.108GlyPhe: 3.108 ± 0.067
4.512GlyGly: 4.512 ± 0.098
1.2GlyHis: 1.2 ± 0.04
5.758GlyIle: 5.758 ± 0.081
4.185GlyLys: 4.185 ± 0.07
7.096GlyLeu: 7.096 ± 0.081
1.568GlyMet: 1.568 ± 0.039
3.127GlyAsn: 3.127 ± 0.084
0.768GlyPro: 0.768 ± 0.03
2.578GlyGln: 2.578 ± 0.057
2.853GlyArg: 2.853 ± 0.064
4.041GlySer: 4.041 ± 0.072
3.862GlyThr: 3.862 ± 0.082
4.591GlyVal: 4.591 ± 0.086
1.05GlyTrp: 1.05 ± 0.04
2.484GlyTyr: 2.484 ± 0.053
0.0GlyXaa: 0.0 ± 0.0
His
1.08HisAla: 1.08 ± 0.035
0.227HisCys: 0.227 ± 0.017
0.867HisAsp: 0.867 ± 0.029
0.954HisGlu: 0.954 ± 0.034
0.723HisPhe: 0.723 ± 0.028
1.145HisGly: 1.145 ± 0.035
0.603HisHis: 0.603 ± 0.027
1.283HisIle: 1.283 ± 0.04
0.885HisLys: 0.885 ± 0.034
2.247HisLeu: 2.247 ± 0.054
0.243HisMet: 0.243 ± 0.017
0.85HisAsn: 0.85 ± 0.033
1.241HisPro: 1.241 ± 0.041
1.024HisGln: 1.024 ± 0.036
1.063HisArg: 1.063 ± 0.032
1.192HisSer: 1.192 ± 0.04
0.929HisThr: 0.929 ± 0.032
0.801HisVal: 0.801 ± 0.028
0.328HisTrp: 0.328 ± 0.017
0.625HisTyr: 0.625 ± 0.022
0.0HisXaa: 0.0 ± 0.0
Ile
8.812IleAla: 8.812 ± 0.11
0.839IleCys: 0.839 ± 0.028
4.31IleAsp: 4.31 ± 0.075
4.252IleGlu: 4.252 ± 0.075
3.059IlePhe: 3.059 ± 0.071
5.251IleGly: 5.251 ± 0.08
1.386IleHis: 1.386 ± 0.041
4.633IleIle: 4.633 ± 0.079
4.048IleLys: 4.048 ± 0.061
7.654IleLeu: 7.654 ± 0.091
1.144IleMet: 1.144 ± 0.034
3.697IleAsn: 3.697 ± 0.07
3.95IlePro: 3.95 ± 0.061
3.146IleGln: 3.146 ± 0.057
3.179IleArg: 3.179 ± 0.063
5.766IleSer: 5.766 ± 0.089
4.673IleThr: 4.673 ± 0.076
4.289IleVal: 4.289 ± 0.063
0.89IleTrp: 0.89 ± 0.031
2.231IleTyr: 2.231 ± 0.054
0.0IleXaa: 0.0 ± 0.0
Lys
4.294LysAla: 4.294 ± 0.077
0.36LysCys: 0.36 ± 0.017
2.46LysAsp: 2.46 ± 0.058
2.778LysGlu: 2.778 ± 0.062
2.205LysPhe: 2.205 ± 0.05
2.841LysGly: 2.841 ± 0.053
0.86LysHis: 0.86 ± 0.028
4.044LysIle: 4.044 ± 0.067
2.262LysLys: 2.262 ± 0.056
5.91LysLeu: 5.91 ± 0.084
0.976LysMet: 0.976 ± 0.032
2.3LysAsn: 2.3 ± 0.051
2.62LysPro: 2.62 ± 0.058
2.597LysGln: 2.597 ± 0.057
2.2LysArg: 2.2 ± 0.048
3.735LysSer: 3.735 ± 0.071
2.923LysThr: 2.923 ± 0.064
3.653LysVal: 3.653 ± 0.061
0.524LysTrp: 0.524 ± 0.024
1.683LysTyr: 1.683 ± 0.044
0.0LysXaa: 0.0 ± 0.0
Leu
9.421LeuAla: 9.421 ± 0.103
0.968LeuCys: 0.968 ± 0.032
5.641LeuAsp: 5.641 ± 0.09
7.541LeuGlu: 7.541 ± 0.117
3.705LeuPhe: 3.705 ± 0.066
8.165LeuGly: 8.165 ± 0.114
1.746LeuHis: 1.746 ± 0.044
7.932LeuIle: 7.932 ± 0.089
6.067LeuLys: 6.067 ± 0.093
10.378LeuLeu: 10.378 ± 0.125
1.978LeuMet: 1.978 ± 0.044
4.921LeuAsn: 4.921 ± 0.084
5.273LeuPro: 5.273 ± 0.086
4.98LeuGln: 4.98 ± 0.084
5.154LeuArg: 5.154 ± 0.077
7.719LeuSer: 7.719 ± 0.101
6.493LeuThr: 6.493 ± 0.101
7.494LeuVal: 7.494 ± 0.095
1.373LeuTrp: 1.373 ± 0.043
2.623LeuTyr: 2.623 ± 0.051
0.0LeuXaa: 0.0 ± 0.0
Met
1.818MetAla: 1.818 ± 0.044
0.126MetCys: 0.126 ± 0.011
0.847MetAsp: 0.847 ± 0.03
1.076MetGlu: 1.076 ± 0.037
0.56MetPhe: 0.56 ± 0.026
1.483MetGly: 1.483 ± 0.037
0.287MetHis: 0.287 ± 0.017
1.36MetIle: 1.36 ± 0.039
0.85MetLys: 0.85 ± 0.027
1.919MetLeu: 1.919 ± 0.047
0.437MetMet: 0.437 ± 0.024
0.869MetAsn: 0.869 ± 0.029
1.061MetPro: 1.061 ± 0.033
0.961MetGln: 0.961 ± 0.033
0.914MetArg: 0.914 ± 0.025
1.402MetSer: 1.402 ± 0.035
1.378MetThr: 1.378 ± 0.042
1.293MetVal: 1.293 ± 0.038
0.158MetTrp: 0.158 ± 0.013
0.406MetTyr: 0.406 ± 0.021
0.0MetXaa: 0.0 ± 0.0
Asn
3.172AsnAla: 3.172 ± 0.064
0.473AsnCys: 0.473 ± 0.02
1.842AsnAsp: 1.842 ± 0.049
1.728AsnGlu: 1.728 ± 0.04
2.113AsnPhe: 2.113 ± 0.058
2.781AsnGly: 2.781 ± 0.068
1.026AsnHis: 1.026 ± 0.04
3.176AsnIle: 3.176 ± 0.062
1.653AsnLys: 1.653 ± 0.048
6.4AsnLeu: 6.4 ± 0.109
0.811AsnMet: 0.811 ± 0.03
2.02AsnAsn: 2.02 ± 0.065
2.994AsnPro: 2.994 ± 0.065
2.808AsnGln: 2.808 ± 0.053
2.243AsnArg: 2.243 ± 0.044
3.315AsnSer: 3.315 ± 0.065
2.346AsnThr: 2.346 ± 0.064
2.234AsnVal: 2.234 ± 0.049
0.773AsnTrp: 0.773 ± 0.028
1.625AsnTyr: 1.625 ± 0.042
0.0AsnXaa: 0.0 ± 0.0
Pro
2.864ProAla: 2.864 ± 0.061
0.297ProCys: 0.297 ± 0.016
2.928ProAsp: 2.928 ± 0.051
3.619ProGlu: 3.619 ± 0.064
1.711ProPhe: 1.711 ± 0.04
1.718ProGly: 1.718 ± 0.044
0.877ProHis: 0.877 ± 0.028
3.483ProIle: 3.483 ± 0.062
2.576ProLys: 2.576 ± 0.051
4.313ProLeu: 4.313 ± 0.08
0.792ProMet: 0.792 ± 0.032
2.555ProAsn: 2.555 ± 0.051
1.824ProPro: 1.824 ± 0.048
2.355ProGln: 2.355 ± 0.052
1.629ProArg: 1.629 ± 0.043
3.11ProSer: 3.11 ± 0.07
2.793ProThr: 2.793 ± 0.071
3.093ProVal: 3.093 ± 0.062
0.556ProTrp: 0.556 ± 0.021
1.288ProTyr: 1.288 ± 0.037
0.0ProXaa: 0.0 ± 0.0
Gln
4.409GlnAla: 4.409 ± 0.073
0.312GlnCys: 0.312 ± 0.019
2.198GlnAsp: 2.198 ± 0.052
3.244GlnGlu: 3.244 ± 0.069
1.718GlnPhe: 1.718 ± 0.04
2.946GlnGly: 2.946 ± 0.058
0.761GlnHis: 0.761 ± 0.029
4.204GlnIle: 4.204 ± 0.069
2.938GlnLys: 2.938 ± 0.071
5.062GlnLeu: 5.062 ± 0.082
0.912GlnMet: 0.912 ± 0.031
2.293GlnAsn: 2.293 ± 0.057
2.184GlnPro: 2.184 ± 0.047
2.583GlnGln: 2.583 ± 0.078
2.316GlnArg: 2.316 ± 0.056
3.116GlnSer: 3.116 ± 0.063
2.84GlnThr: 2.84 ± 0.06
3.858GlnVal: 3.858 ± 0.069
0.632GlnTrp: 0.632 ± 0.024
1.188GlnTyr: 1.188 ± 0.036
0.0GlnXaa: 0.0 ± 0.0
Arg
3.063ArgAla: 3.063 ± 0.056
0.446ArgCys: 0.446 ± 0.021
2.433ArgAsp: 2.433 ± 0.049
2.657ArgGlu: 2.657 ± 0.054
2.104ArgPhe: 2.104 ± 0.044
2.687ArgGly: 2.687 ± 0.055
0.919ArgHis: 0.919 ± 0.031
3.427ArgIle: 3.427 ± 0.055
2.258ArgLys: 2.258 ± 0.049
5.61ArgLeu: 5.61 ± 0.072
0.958ArgMet: 0.958 ± 0.037
2.11ArgAsn: 2.11 ± 0.045
1.896ArgPro: 1.896 ± 0.046
2.694ArgGln: 2.694 ± 0.053
2.525ArgArg: 2.525 ± 0.058
3.632ArgSer: 3.632 ± 0.067
2.406ArgThr: 2.406 ± 0.045
2.95ArgVal: 2.95 ± 0.06
0.682ArgTrp: 0.682 ± 0.026
1.7ArgTyr: 1.7 ± 0.043
0.0ArgXaa: 0.0 ± 0.0
Ser
4.569SerAla: 4.569 ± 0.086
0.627SerCys: 0.627 ± 0.028
3.94SerAsp: 3.94 ± 0.075
4.0SerGlu: 4.0 ± 0.063
2.583SerPhe: 2.583 ± 0.053
4.984SerGly: 4.984 ± 0.084
1.361SerHis: 1.361 ± 0.038
4.481SerIle: 4.481 ± 0.073
3.227SerLys: 3.227 ± 0.057
7.818SerLeu: 7.818 ± 0.1
1.409SerMet: 1.409 ± 0.037
3.065SerAsn: 3.065 ± 0.073
3.297SerPro: 3.297 ± 0.059
3.658SerGln: 3.658 ± 0.061
2.962SerArg: 2.962 ± 0.054
4.791SerSer: 4.791 ± 0.086
3.647SerThr: 3.647 ± 0.071
4.03SerVal: 4.03 ± 0.07
0.822SerTrp: 0.822 ± 0.034
2.033SerTyr: 2.033 ± 0.045
0.0SerXaa: 0.0 ± 0.0
Thr
4.729ThrAla: 4.729 ± 0.078
0.53ThrCys: 0.53 ± 0.027
2.865ThrAsp: 2.865 ± 0.067
3.401ThrGlu: 3.401 ± 0.061
2.096ThrPhe: 2.096 ± 0.048
4.313ThrGly: 4.313 ± 0.095
0.982ThrHis: 0.982 ± 0.032
4.11ThrIle: 4.11 ± 0.061
2.96ThrLys: 2.96 ± 0.061
6.078ThrLeu: 6.078 ± 0.104
0.936ThrMet: 0.936 ± 0.029
2.593ThrAsn: 2.593 ± 0.061
2.996ThrPro: 2.996 ± 0.072
2.53ThrGln: 2.53 ± 0.058
2.216ThrArg: 2.216 ± 0.047
3.712ThrSer: 3.712 ± 0.077
3.284ThrThr: 3.284 ± 0.082
3.987ThrVal: 3.987 ± 0.077
0.753ThrTrp: 0.753 ± 0.03
1.664ThrTyr: 1.664 ± 0.036
0.0ThrXaa: 0.0 ± 0.0
Val
5.295ValAla: 5.295 ± 0.082
0.692ValCys: 0.692 ± 0.029
3.318ValAsp: 3.318 ± 0.058
3.832ValGlu: 3.832 ± 0.061
2.463ValPhe: 2.463 ± 0.055
4.661ValGly: 4.661 ± 0.078
0.961ValHis: 0.961 ± 0.037
5.431ValIle: 5.431 ± 0.08
3.563ValLys: 3.563 ± 0.072
6.636ValLeu: 6.636 ± 0.094
1.394ValMet: 1.394 ± 0.035
3.132ValAsn: 3.132 ± 0.068
2.656ValPro: 2.656 ± 0.058
2.55ValGln: 2.55 ± 0.052
2.892ValArg: 2.892 ± 0.056
4.324ValSer: 4.324 ± 0.067
3.997ValThr: 3.997 ± 0.071
4.489ValVal: 4.489 ± 0.082
0.869ValTrp: 0.869 ± 0.031
1.723ValTyr: 1.723 ± 0.042
0.0ValXaa: 0.0 ± 0.0
Trp
0.919TrpAla: 0.919 ± 0.028
0.123TrpCys: 0.123 ± 0.011
0.617TrpAsp: 0.617 ± 0.028
0.816TrpGlu: 0.816 ± 0.03
0.593TrpPhe: 0.593 ± 0.028
0.898TrpGly: 0.898 ± 0.032
0.379TrpHis: 0.379 ± 0.024
0.902TrpIle: 0.902 ± 0.033
0.523TrpLys: 0.523 ± 0.024
1.822TrpLeu: 1.822 ± 0.044
0.295TrpMet: 0.295 ± 0.017
0.56TrpAsn: 0.56 ± 0.026
0.082TrpPro: 0.082 ± 0.01
1.345TrpGln: 1.345 ± 0.039
0.758TrpArg: 0.758 ± 0.029
0.73TrpSer: 0.73 ± 0.03
0.572TrpThr: 0.572 ± 0.025
0.832TrpVal: 0.832 ± 0.03
0.216TrpTrp: 0.216 ± 0.018
0.395TrpTyr: 0.395 ± 0.021
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.331TyrAla: 2.331 ± 0.048
0.347TyrCys: 0.347 ± 0.021
1.489TyrAsp: 1.489 ± 0.042
1.717TyrGlu: 1.717 ± 0.043
1.368TyrPhe: 1.368 ± 0.037
2.16TyrGly: 2.16 ± 0.051
0.617TyrHis: 0.617 ± 0.029
1.928TyrIle: 1.928 ± 0.042
1.354TyrLys: 1.354 ± 0.04
3.696TyrLeu: 3.696 ± 0.071
0.52TyrMet: 0.52 ± 0.023
1.153TyrAsn: 1.153 ± 0.037
1.632TyrPro: 1.632 ± 0.04
1.774TyrGln: 1.774 ± 0.05
1.791TyrArg: 1.791 ± 0.05
1.889TyrSer: 1.889 ± 0.041
1.469TyrThr: 1.469 ± 0.038
1.724TyrVal: 1.724 ± 0.044
0.464TyrTrp: 0.464 ± 0.023
1.022TyrTyr: 1.022 ± 0.033
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 3310 proteins (995375 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski