Methylomonas sp. DH-1

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Methylococcales; Methylococcaceae; Methylomonas; unclassified Methylomonas

Average proteome isoelectric point is 6.37

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4316 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A172UAZ0|A0A172UAZ0_9GAMM Inositol-1-monophosphatase OS=Methylomonas sp. DH-1 OX=1727196 GN=AYM39_15700 PE=3 SV=1
MM1 pKa = 7.85ADD3 pKa = 3.19YY4 pKa = 8.69TAAPGAAGDD13 pKa = 3.95EE14 pKa = 4.27AVVGQFDD21 pKa = 4.0TYY23 pKa = 11.65DD24 pKa = 3.28FGLGVGLVKK33 pKa = 10.84VNNDD37 pKa = 2.84GTLNGYY43 pKa = 7.96FQTYY47 pKa = 9.96VNDD50 pKa = 4.86HH51 pKa = 6.78ILTNSGGINVPQLNVSGASGSGSGFEE77 pKa = 4.18LTVVASFSGTYY88 pKa = 9.84SVLPGGLQSFSLTAGNVGLYY108 pKa = 10.36FDD110 pKa = 4.42TTPDD114 pKa = 3.48FNFGADD120 pKa = 2.87NGFNDD125 pKa = 4.77GSAILTGTITGGGGFISLASGTGIEE150 pKa = 4.05QLDD153 pKa = 4.2LNFSGIFGNSDD164 pKa = 3.38ANVYY168 pKa = 9.7SQAIGGGSALFSIDD182 pKa = 4.1LKK184 pKa = 11.38NSTLLPGIDD193 pKa = 3.81SVLGHH198 pKa = 6.33NKK200 pKa = 9.96SEE202 pKa = 4.18GALAAVDD209 pKa = 4.03GSINLTAVPLPPAVWMFGAGLAGLLGVGQRR239 pKa = 11.84KK240 pKa = 8.62KK241 pKa = 11.07ASAA244 pKa = 3.42

Molecular weight: 24.38 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A172UD73|A0A172UD73_9GAMM Uncharacterized protein OS=Methylomonas sp. DH-1 OX=1727196 GN=AYM39_18150 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.17QPSKK9 pKa = 8.35IKK11 pKa = 10.26RR12 pKa = 11.84VRR14 pKa = 11.84THH16 pKa = 5.81GFRR19 pKa = 11.84ARR21 pKa = 11.84MATVGGRR28 pKa = 11.84KK29 pKa = 9.26VINARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.12GRR39 pKa = 11.84ASLTVV44 pKa = 3.04

Molecular weight: 5.01 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4316

0

4316

1443716

24

10850

334.5

36.82

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.067 ± 0.063

1.005 ± 0.015

5.61 ± 0.035

5.718 ± 0.041

3.954 ± 0.027

7.547 ± 0.063

2.172 ± 0.02

5.439 ± 0.029

4.278 ± 0.04

11.052 ± 0.057

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.125 ± 0.02

3.729 ± 0.044

4.507 ± 0.03

4.595 ± 0.034

5.864 ± 0.043

5.746 ± 0.034

4.712 ± 0.041

6.668 ± 0.037

1.345 ± 0.017

2.866 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski