Amino acid dipepetide frequency for Methylomonas sp. DH-1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
14.58AlaAla: 14.58 ± 0.215
1.139AlaCys: 1.139 ± 0.031
6.904AlaAsp: 6.904 ± 0.097
8.113AlaGlu: 8.113 ± 0.096
3.726AlaPhe: 3.726 ± 0.051
9.435AlaGly: 9.435 ± 0.166
1.93AlaHis: 1.93 ± 0.037
5.89AlaIle: 5.89 ± 0.07
4.777AlaLys: 4.777 ± 0.073
12.084AlaLeu: 12.084 ± 0.135
2.714AlaMet: 2.714 ± 0.049
3.983AlaAsn: 3.983 ± 0.082
3.989AlaPro: 3.989 ± 0.065
4.335AlaGln: 4.335 ± 0.058
5.177AlaArg: 5.177 ± 0.065
5.404AlaSer: 5.404 ± 0.075
4.718AlaThr: 4.718 ± 0.061
7.524AlaVal: 7.524 ± 0.083
1.511AlaTrp: 1.511 ± 0.036
2.74AlaTyr: 2.74 ± 0.045
0.0AlaXaa: 0.0 ± 0.0
Cys
0.982CysAla: 0.982 ± 0.028
0.159CysCys: 0.159 ± 0.01
0.538CysAsp: 0.538 ± 0.018
0.519CysGlu: 0.519 ± 0.02
0.405CysPhe: 0.405 ± 0.017
0.959CysGly: 0.959 ± 0.031
0.296CysHis: 0.296 ± 0.014
0.468CysIle: 0.468 ± 0.019
0.34CysLys: 0.34 ± 0.017
1.075CysLeu: 1.075 ± 0.029
0.167CysMet: 0.167 ± 0.011
0.332CysAsn: 0.332 ± 0.016
0.477CysPro: 0.477 ± 0.022
0.398CysGln: 0.398 ± 0.019
0.814CysArg: 0.814 ± 0.024
0.646CysSer: 0.646 ± 0.023
0.413CysThr: 0.413 ± 0.018
0.608CysVal: 0.608 ± 0.021
0.141CysTrp: 0.141 ± 0.012
0.316CysTyr: 0.316 ± 0.015
0.0CysXaa: 0.0 ± 0.0
Asp
5.917AspAla: 5.917 ± 0.08
0.618AspCys: 0.618 ± 0.023
3.0AspAsp: 3.0 ± 0.059
3.1AspGlu: 3.1 ± 0.05
2.525AspPhe: 2.525 ± 0.045
4.451AspGly: 4.451 ± 0.106
1.057AspHis: 1.057 ± 0.028
3.517AspIle: 3.517 ± 0.049
2.602AspLys: 2.602 ± 0.054
5.672AspLeu: 5.672 ± 0.063
1.206AspMet: 1.206 ± 0.033
2.007AspAsn: 2.007 ± 0.046
2.64AspPro: 2.64 ± 0.045
2.129AspGln: 2.129 ± 0.046
3.14AspArg: 3.14 ± 0.052
3.312AspSer: 3.312 ± 0.051
2.64AspThr: 2.64 ± 0.082
3.404AspVal: 3.404 ± 0.06
1.049AspTrp: 1.049 ± 0.028
2.111AspTyr: 2.111 ± 0.039
0.0AspXaa: 0.0 ± 0.0
Glu
5.733GluAla: 5.733 ± 0.063
0.448GluCys: 0.448 ± 0.02
2.402GluAsp: 2.402 ± 0.046
2.59GluGlu: 2.59 ± 0.063
2.373GluPhe: 2.373 ± 0.046
2.892GluGly: 2.892 ± 0.05
1.491GluHis: 1.491 ± 0.038
3.835GluIle: 3.835 ± 0.06
2.879GluLys: 2.879 ± 0.065
7.038GluLeu: 7.038 ± 0.091
1.378GluMet: 1.378 ± 0.031
2.16GluAsn: 2.16 ± 0.037
2.266GluPro: 2.266 ± 0.045
3.652GluGln: 3.652 ± 0.064
4.121GluArg: 4.121 ± 0.068
2.931GluSer: 2.931 ± 0.051
3.188GluThr: 3.188 ± 0.05
3.49GluVal: 3.49 ± 0.06
0.754GluTrp: 0.754 ± 0.025
1.559GluTyr: 1.559 ± 0.033
0.0GluXaa: 0.0 ± 0.0
Phe
4.254PheAla: 4.254 ± 0.052
0.519PheCys: 0.519 ± 0.019
2.684PheAsp: 2.684 ± 0.048
2.255PheGlu: 2.255 ± 0.042
1.681PhePhe: 1.681 ± 0.041
3.506PheGly: 3.506 ± 0.058
0.738PheHis: 0.738 ± 0.021
1.947PheIle: 1.947 ± 0.044
1.593PheLys: 1.593 ± 0.035
3.58PheLeu: 3.58 ± 0.06
0.794PheMet: 0.794 ± 0.023
1.732PheAsn: 1.732 ± 0.034
1.535PhePro: 1.535 ± 0.033
1.376PheGln: 1.376 ± 0.028
2.063PheArg: 2.063 ± 0.042
2.868PheSer: 2.868 ± 0.053
1.849PheThr: 1.849 ± 0.039
2.71PheVal: 2.71 ± 0.048
0.587PheTrp: 0.587 ± 0.022
1.265PheTyr: 1.265 ± 0.03
0.0PheXaa: 0.0 ± 0.0
Gly
6.586GlyAla: 6.586 ± 0.13
0.963GlyCys: 0.963 ± 0.031
4.211GlyAsp: 4.211 ± 0.108
4.335GlyGlu: 4.335 ± 0.066
3.53GlyPhe: 3.53 ± 0.055
5.901GlyGly: 5.901 ± 0.165
1.654GlyHis: 1.654 ± 0.037
4.481GlyIle: 4.481 ± 0.057
3.918GlyLys: 3.918 ± 0.053
8.229GlyLeu: 8.229 ± 0.091
1.852GlyMet: 1.852 ± 0.04
3.098GlyAsn: 3.098 ± 0.146
2.1GlyPro: 2.1 ± 0.041
3.267GlyGln: 3.267 ± 0.057
4.37GlyArg: 4.37 ± 0.064
4.67GlySer: 4.67 ± 0.11
3.244GlyThr: 3.244 ± 0.067
5.295GlyVal: 5.295 ± 0.08
1.105GlyTrp: 1.105 ± 0.032
2.662GlyTyr: 2.662 ± 0.049
0.0GlyXaa: 0.0 ± 0.0
His
2.097HisAla: 2.097 ± 0.042
0.326HisCys: 0.326 ± 0.018
1.191HisAsp: 1.191 ± 0.029
1.078HisGlu: 1.078 ± 0.03
1.001HisPhe: 1.001 ± 0.027
1.738HisGly: 1.738 ± 0.036
0.619HisHis: 0.619 ± 0.027
1.189HisIle: 1.189 ± 0.025
0.851HisLys: 0.851 ± 0.027
2.236HisLeu: 2.236 ± 0.042
0.408HisMet: 0.408 ± 0.019
0.784HisAsn: 0.784 ± 0.025
1.294HisPro: 1.294 ± 0.033
0.904HisGln: 0.904 ± 0.027
1.339HisArg: 1.339 ± 0.032
1.305HisSer: 1.305 ± 0.036
0.901HisThr: 0.901 ± 0.026
1.2HisVal: 1.2 ± 0.033
0.411HisTrp: 0.411 ± 0.018
0.855HisTyr: 0.855 ± 0.025
0.0HisXaa: 0.0 ± 0.0
Ile
6.446IleAla: 6.446 ± 0.074
0.546IleCys: 0.546 ± 0.022
3.894IleAsp: 3.894 ± 0.058
3.84IleGlu: 3.84 ± 0.06
1.757IlePhe: 1.757 ± 0.036
4.772IleGly: 4.772 ± 0.059
1.112IleHis: 1.112 ± 0.027
2.304IleIle: 2.304 ± 0.05
2.491IleLys: 2.491 ± 0.048
4.82IleLeu: 4.82 ± 0.067
0.922IleMet: 0.922 ± 0.03
2.215IleAsn: 2.215 ± 0.044
2.454IlePro: 2.454 ± 0.051
1.863IleGln: 1.863 ± 0.039
3.161IleArg: 3.161 ± 0.055
3.363IleSer: 3.363 ± 0.052
2.499IleThr: 2.499 ± 0.047
3.906IleVal: 3.906 ± 0.057
0.586IleTrp: 0.586 ± 0.018
1.438IleTyr: 1.438 ± 0.032
0.0IleXaa: 0.0 ± 0.0
Lys
4.42LysAla: 4.42 ± 0.066
0.27LysCys: 0.27 ± 0.014
2.099LysAsp: 2.099 ± 0.049
1.985LysGlu: 1.985 ± 0.048
1.27LysPhe: 1.27 ± 0.031
2.476LysGly: 2.476 ± 0.053
1.045LysHis: 1.045 ± 0.03
2.694LysIle: 2.694 ± 0.053
2.047LysLys: 2.047 ± 0.055
4.991LysLeu: 4.991 ± 0.067
0.97LysMet: 0.97 ± 0.025
1.728LysAsn: 1.728 ± 0.04
2.547LysPro: 2.547 ± 0.05
2.521LysGln: 2.521 ± 0.055
2.522LysArg: 2.522 ± 0.05
2.355LysSer: 2.355 ± 0.041
2.717LysThr: 2.717 ± 0.049
2.656LysVal: 2.656 ± 0.051
0.411LysTrp: 0.411 ± 0.016
1.059LysTyr: 1.059 ± 0.031
0.0LysXaa: 0.0 ± 0.0
Leu
13.915LeuAla: 13.915 ± 0.156
1.072LeuCys: 1.072 ± 0.031
6.156LeuAsp: 6.156 ± 0.068
6.088LeuGlu: 6.088 ± 0.084
4.297LeuPhe: 4.297 ± 0.061
7.506LeuGly: 7.506 ± 0.085
2.373LeuHis: 2.373 ± 0.04
5.36LeuIle: 5.36 ± 0.075
4.753LeuLys: 4.753 ± 0.075
12.441LeuLeu: 12.441 ± 0.134
2.117LeuMet: 2.117 ± 0.041
4.2LeuAsn: 4.2 ± 0.064
5.786LeuPro: 5.786 ± 0.068
5.165LeuGln: 5.165 ± 0.071
6.308LeuArg: 6.308 ± 0.085
6.922LeuSer: 6.922 ± 0.084
5.322LeuThr: 5.322 ± 0.113
6.85LeuVal: 6.85 ± 0.07
1.2LeuTrp: 1.2 ± 0.03
2.685LeuTyr: 2.685 ± 0.044
0.0LeuXaa: 0.0 ± 0.0
Met
2.642MetAla: 2.642 ± 0.05
0.123MetCys: 0.123 ± 0.009
1.042MetAsp: 1.042 ± 0.029
0.986MetGlu: 0.986 ± 0.026
0.657MetPhe: 0.657 ± 0.019
1.319MetGly: 1.319 ± 0.033
0.458MetHis: 0.458 ± 0.016
0.988MetIle: 0.988 ± 0.026
0.995MetLys: 0.995 ± 0.031
2.491MetLeu: 2.491 ± 0.04
0.482MetMet: 0.482 ± 0.018
0.885MetAsn: 0.885 ± 0.027
1.243MetPro: 1.243 ± 0.028
1.094MetGln: 1.094 ± 0.031
1.263MetArg: 1.263 ± 0.029
1.385MetSer: 1.385 ± 0.034
1.216MetThr: 1.216 ± 0.032
1.46MetVal: 1.46 ± 0.036
0.143MetTrp: 0.143 ± 0.009
0.381MetTyr: 0.381 ± 0.019
0.0MetXaa: 0.0 ± 0.0
Asn
3.782AsnAla: 3.782 ± 0.063
0.373AsnCys: 0.373 ± 0.017
2.032AsnAsp: 2.032 ± 0.09
1.599AsnGlu: 1.599 ± 0.034
1.378AsnPhe: 1.378 ± 0.039
3.059AsnGly: 3.059 ± 0.08
0.795AsnHis: 0.795 ± 0.027
2.121AsnIle: 2.121 ± 0.047
1.448AsnLys: 1.448 ± 0.035
4.083AsnLeu: 4.083 ± 0.064
0.731AsnMet: 0.731 ± 0.024
1.397AsnAsn: 1.397 ± 0.047
2.348AsnPro: 2.348 ± 0.043
1.787AsnGln: 1.787 ± 0.039
2.546AsnArg: 2.546 ± 0.047
2.108AsnSer: 2.108 ± 0.055
1.732AsnThr: 1.732 ± 0.054
2.313AsnVal: 2.313 ± 0.054
0.537AsnTrp: 0.537 ± 0.019
1.122AsnTyr: 1.122 ± 0.03
0.0AsnXaa: 0.0 ± 0.0
Pro
5.704ProAla: 5.704 ± 0.079
0.38ProCys: 0.38 ± 0.014
3.048ProAsp: 3.048 ± 0.044
3.396ProGlu: 3.396 ± 0.052
1.703ProPhe: 1.703 ± 0.035
3.539ProGly: 3.539 ± 0.054
0.891ProHis: 0.891 ± 0.026
2.216ProIle: 2.216 ± 0.04
1.712ProLys: 1.712 ± 0.037
4.805ProLeu: 4.805 ± 0.075
0.898ProMet: 0.898 ± 0.027
1.568ProAsn: 1.568 ± 0.036
1.906ProPro: 1.906 ± 0.047
1.973ProGln: 1.973 ± 0.037
1.862ProArg: 1.862 ± 0.041
2.245ProSer: 2.245 ± 0.044
1.977ProThr: 1.977 ± 0.038
3.469ProVal: 3.469 ± 0.055
0.566ProTrp: 0.566 ± 0.021
1.215ProTyr: 1.215 ± 0.03
0.0ProXaa: 0.0 ± 0.0
Gln
5.888GlnAla: 5.888 ± 0.08
0.33GlnCys: 0.33 ± 0.017
1.977GlnAsp: 1.977 ± 0.044
1.974GlnGlu: 1.974 ± 0.044
1.745GlnPhe: 1.745 ± 0.039
2.888GlnGly: 2.888 ± 0.047
1.201GlnHis: 1.201 ± 0.032
2.697GlnIle: 2.697 ± 0.077
1.694GlnLys: 1.694 ± 0.04
5.032GlnLeu: 5.032 ± 0.072
0.912GlnMet: 0.912 ± 0.024
1.523GlnAsn: 1.523 ± 0.031
2.218GlnPro: 2.218 ± 0.042
2.892GlnGln: 2.892 ± 0.066
3.287GlnArg: 3.287 ± 0.066
2.489GlnSer: 2.489 ± 0.044
2.489GlnThr: 2.489 ± 0.045
2.909GlnVal: 2.909 ± 0.065
0.598GlnTrp: 0.598 ± 0.021
1.204GlnTyr: 1.204 ± 0.032
0.0GlnXaa: 0.0 ± 0.0
Arg
4.86ArgAla: 4.86 ± 0.068
0.596ArgCys: 0.596 ± 0.023
3.179ArgAsp: 3.179 ± 0.046
3.497ArgGlu: 3.497 ± 0.054
2.777ArgPhe: 2.777 ± 0.044
3.486ArgGly: 3.486 ± 0.05
1.621ArgHis: 1.621 ± 0.036
3.697ArgIle: 3.697 ± 0.059
2.429ArgLys: 2.429 ± 0.052
7.125ArgLeu: 7.125 ± 0.09
1.36ArgMet: 1.36 ± 0.033
2.24ArgAsn: 2.24 ± 0.038
2.43ArgPro: 2.43 ± 0.044
3.472ArgGln: 3.472 ± 0.065
3.951ArgArg: 3.951 ± 0.075
3.123ArgSer: 3.123 ± 0.048
2.277ArgThr: 2.277 ± 0.041
3.551ArgVal: 3.551 ± 0.059
0.934ArgTrp: 0.934 ± 0.03
2.039ArgTyr: 2.039 ± 0.035
0.0ArgXaa: 0.0 ± 0.0
Ser
6.426SerAla: 6.426 ± 0.08
0.551SerCys: 0.551 ± 0.02
3.021SerAsp: 3.021 ± 0.06
3.233SerGlu: 3.233 ± 0.055
2.381SerPhe: 2.381 ± 0.049
5.494SerGly: 5.494 ± 0.096
1.295SerHis: 1.295 ± 0.035
3.071SerIle: 3.071 ± 0.049
2.163SerLys: 2.163 ± 0.046
6.309SerLeu: 6.309 ± 0.072
1.135SerMet: 1.135 ± 0.029
1.959SerAsn: 1.959 ± 0.044
2.374SerPro: 2.374 ± 0.039
2.377SerGln: 2.377 ± 0.043
3.381SerArg: 3.381 ± 0.051
3.267SerSer: 3.267 ± 0.061
2.444SerThr: 2.444 ± 0.065
4.139SerVal: 4.139 ± 0.059
0.742SerTrp: 0.742 ± 0.025
1.698SerTyr: 1.698 ± 0.042
0.0SerXaa: 0.0 ± 0.0
Thr
5.271ThrAla: 5.271 ± 0.084
0.382ThrCys: 0.382 ± 0.017
2.649ThrAsp: 2.649 ± 0.056
2.631ThrGlu: 2.631 ± 0.046
1.694ThrPhe: 1.694 ± 0.039
4.223ThrGly: 4.223 ± 0.083
0.979ThrHis: 0.979 ± 0.023
2.53ThrIle: 2.53 ± 0.045
1.558ThrLys: 1.558 ± 0.04
5.833ThrLeu: 5.833 ± 0.075
0.862ThrMet: 0.862 ± 0.022
1.423ThrAsn: 1.423 ± 0.038
2.721ThrPro: 2.721 ± 0.05
1.856ThrGln: 1.856 ± 0.06
2.328ThrArg: 2.328 ± 0.041
2.386ThrSer: 2.386 ± 0.072
2.323ThrThr: 2.323 ± 0.096
3.79ThrVal: 3.79 ± 0.064
0.543ThrTrp: 0.543 ± 0.02
1.141ThrTyr: 1.141 ± 0.037
0.0ThrXaa: 0.0 ± 0.0
Val
7.483ValAla: 7.483 ± 0.092
0.743ValCys: 0.743 ± 0.026
3.862ValAsp: 3.862 ± 0.059
4.087ValGlu: 4.087 ± 0.063
2.73ValPhe: 2.73 ± 0.05
4.838ValGly: 4.838 ± 0.069
1.191ValHis: 1.191 ± 0.029
3.515ValIle: 3.515 ± 0.049
3.094ValLys: 3.094 ± 0.047
7.071ValLeu: 7.071 ± 0.074
1.589ValMet: 1.589 ± 0.039
2.668ValAsn: 2.668 ± 0.061
2.697ValPro: 2.697 ± 0.042
2.307ValGln: 2.307 ± 0.046
3.51ValArg: 3.51 ± 0.048
4.156ValSer: 4.156 ± 0.061
3.393ValThr: 3.393 ± 0.064
4.96ValVal: 4.96 ± 0.081
0.918ValTrp: 0.918 ± 0.024
1.865ValTyr: 1.865 ± 0.038
0.0ValXaa: 0.0 ± 0.0
Trp
1.02TrpAla: 1.02 ± 0.028
0.141TrpCys: 0.141 ± 0.011
0.621TrpAsp: 0.621 ± 0.021
0.596TrpGlu: 0.596 ± 0.023
0.598TrpPhe: 0.598 ± 0.022
0.779TrpGly: 0.779 ± 0.027
0.38TrpHis: 0.38 ± 0.016
0.657TrpIle: 0.657 ± 0.02
0.427TrpLys: 0.427 ± 0.017
2.135TrpLeu: 2.135 ± 0.041
0.296TrpMet: 0.296 ± 0.014
0.486TrpAsn: 0.486 ± 0.019
0.616TrpPro: 0.616 ± 0.021
0.971TrpGln: 0.971 ± 0.03
1.062TrpArg: 1.062 ± 0.033
0.762TrpSer: 0.762 ± 0.027
0.549TrpThr: 0.549 ± 0.018
0.808TrpVal: 0.808 ± 0.026
0.211TrpTrp: 0.211 ± 0.011
0.336TrpTyr: 0.336 ± 0.018
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.669TyrAla: 2.669 ± 0.043
0.374TyrCys: 0.374 ± 0.016
1.587TyrAsp: 1.587 ± 0.043
1.279TyrGlu: 1.279 ± 0.033
1.306TyrPhe: 1.306 ± 0.032
2.21TyrGly: 2.21 ± 0.044
0.598TyrHis: 0.598 ± 0.02
1.212TyrIle: 1.212 ± 0.035
1.013TyrLys: 1.013 ± 0.03
3.467TyrLeu: 3.467 ± 0.055
0.458TyrMet: 0.458 ± 0.02
0.903TyrAsn: 0.903 ± 0.028
1.432TyrPro: 1.432 ± 0.035
1.61TyrGln: 1.61 ± 0.034
2.44TyrArg: 2.44 ± 0.047
1.764TyrSer: 1.764 ± 0.036
1.232TyrThr: 1.232 ± 0.033
1.637TyrVal: 1.637 ± 0.034
0.501TyrTrp: 0.501 ± 0.019
0.969TyrTyr: 0.969 ± 0.027
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 4316 proteins (1443716 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski