Domibacillus enclensis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Domibacillus

Average proteome isoelectric point is 6.1

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3765 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1N7B4F1|A0A1N7B4F1_9BACI Uncharacterized protein OS=Domibacillus enclensis OX=1017273 GN=SAMN05443094_108139 PE=3 SV=1
MM1 pKa = 7.4KK2 pKa = 10.2KK3 pKa = 10.0IIIFAVVLIAIFGAIAFITQYY24 pKa = 10.74QKK26 pKa = 11.14EE27 pKa = 4.26QATEE31 pKa = 3.93GNPYY35 pKa = 10.79GKK37 pKa = 9.91DD38 pKa = 3.4DD39 pKa = 4.84LDD41 pKa = 4.3ASTIEE46 pKa = 4.41QLDD49 pKa = 3.68DD50 pKa = 4.02PNYY53 pKa = 10.13QNIVLPEE60 pKa = 4.19DD61 pKa = 3.54LQEE64 pKa = 4.21KK65 pKa = 10.25LEE67 pKa = 4.35NGEE70 pKa = 4.0DD71 pKa = 3.5AVVYY75 pKa = 8.67FYY77 pKa = 11.55SPTCPHH83 pKa = 6.38CQEE86 pKa = 4.27TTPVLMPIADD96 pKa = 4.28DD97 pKa = 4.08EE98 pKa = 4.54NVDD101 pKa = 3.62VLQYY105 pKa = 11.53NLLEE109 pKa = 4.64FEE111 pKa = 4.96QGWTDD116 pKa = 3.26YY117 pKa = 10.72KK118 pKa = 10.64IEE120 pKa = 4.01YY121 pKa = 8.81TPTLIYY127 pKa = 10.56FEE129 pKa = 4.79NGVEE133 pKa = 4.1KK134 pKa = 10.79DD135 pKa = 3.7RR136 pKa = 11.84LVGSQPEE143 pKa = 4.45SEE145 pKa = 3.77WTAFLGQTKK154 pKa = 10.26EE155 pKa = 4.08EE156 pKa = 4.23NQQ158 pKa = 3.31

Molecular weight: 18.08 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1N6YYB9|A0A1N6YYB9_9BACI Single-stranded DNA-binding protein OS=Domibacillus enclensis OX=1017273 GN=SAMN05443094_10625 PE=3 SV=1
MM1 pKa = 7.44KK2 pKa = 9.59RR3 pKa = 11.84TFQPNKK9 pKa = 8.24RR10 pKa = 11.84KK11 pKa = 9.6RR12 pKa = 11.84SKK14 pKa = 9.83VHH16 pKa = 5.77GFRR19 pKa = 11.84ARR21 pKa = 11.84MSSKK25 pKa = 9.98NGRR28 pKa = 11.84RR29 pKa = 11.84VLASRR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.65GRR39 pKa = 11.84KK40 pKa = 8.74VLSAA44 pKa = 4.05

Molecular weight: 5.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3765

0

3765

1138485

25

2263

302.4

33.66

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.569 ± 0.046

0.688 ± 0.011

5.25 ± 0.03

7.564 ± 0.042

4.502 ± 0.028

7.189 ± 0.037

2.147 ± 0.022

6.882 ± 0.039

6.053 ± 0.034

9.5 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.982 ± 0.019

3.673 ± 0.029

3.796 ± 0.021

3.829 ± 0.026

4.346 ± 0.026

5.999 ± 0.031

5.509 ± 0.027

7.255 ± 0.032

1.063 ± 0.015

3.203 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski