Stenotrophomonas chelatiphaga
Average proteome isoelectric point is 6.57
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3376 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0R0CU18|A0A0R0CU18_9GAMM LuxR family transcriptional regulator OS=Stenotrophomonas chelatiphaga OX=517011 GN=ABB28_10860 PE=4 SV=1
MM1 pKa = 7.21 ATEE4 pKa = 4.85 CIEE7 pKa = 3.9 GRR9 pKa = 11.84 HH10 pKa = 5.51 FEE12 pKa = 4.53 GEE14 pKa = 4.31 HH15 pKa = 6.25 PMSDD19 pKa = 3.21 TPPAVFRR26 pKa = 11.84 TWMCVVCGFLYY37 pKa = 10.75 HH38 pKa = 6.74 EE39 pKa = 5.09 ADD41 pKa = 3.65 GLPEE45 pKa = 4.0 EE46 pKa = 5.59 GIAAGTRR53 pKa = 11.84 WEE55 pKa = 4.73 DD56 pKa = 4.32 VPDD59 pKa = 3.14 TWTCPDD65 pKa = 3.91 CGVTKK70 pKa = 10.7 DD71 pKa = 3.65 DD72 pKa = 4.21 FEE74 pKa = 4.39 MVEE77 pKa = 4.23 LDD79 pKa = 3.29
Molecular weight: 8.88 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.815
IPC2_protein 4.05
IPC_protein 3.961
Toseland 3.783
ProMoST 4.024
Dawson 3.935
Bjellqvist 4.139
Wikipedia 3.834
Rodwell 3.795
Grimsley 3.694
Solomon 3.923
Lehninger 3.872
Nozaki 4.05
DTASelect 4.215
Thurlkill 3.821
EMBOSS 3.846
Sillero 4.075
Patrickios 0.896
IPC_peptide 3.923
IPC2_peptide 4.062
IPC2.peptide.svr19 4.008
Protein with the highest isoelectric point:
>tr|A0A0R0D4G3|A0A0R0D4G3_9GAMM Membrane protein OS=Stenotrophomonas chelatiphaga OX=517011 GN=ABB28_13950 PE=4 SV=1
MM1 pKa = 7.51 RR2 pKa = 11.84 QLSLADD8 pKa = 3.7 RR9 pKa = 11.84 GQTISLDD16 pKa = 3.66 KK17 pKa = 11.3 SPDD20 pKa = 3.53 DD21 pKa = 3.82 ATPICLSVARR31 pKa = 11.84 LGSLQTAGSCFTVWIQVRR49 pKa = 11.84 GSAWVEE55 pKa = 3.64 AKK57 pKa = 10.12 EE58 pKa = 4.14 GRR60 pKa = 11.84 FRR62 pKa = 11.84 MRR64 pKa = 11.84 RR65 pKa = 11.84 GEE67 pKa = 3.87 WIAFEE72 pKa = 4.34 KK73 pKa = 10.51 EE74 pKa = 3.93 SRR76 pKa = 11.84 PLVQAGRR83 pKa = 11.84 SGLCIGLALNADD95 pKa = 4.2 AMRR98 pKa = 11.84 MLMEE102 pKa = 5.06 LADD105 pKa = 4.17 CGLYY109 pKa = 10.38 AGRR112 pKa = 11.84 GLMKK116 pKa = 10.28 QAEE119 pKa = 4.41 LRR121 pKa = 11.84 VALRR125 pKa = 11.84 LWRR128 pKa = 11.84 DD129 pKa = 3.32 ALDD132 pKa = 3.8 SGLPAQALRR141 pKa = 11.84 PMLLHH146 pKa = 6.77 LASLQRR152 pKa = 11.84 TMAGTVQRR160 pKa = 11.84 CPGRR164 pKa = 11.84 SRR166 pKa = 11.84 SRR168 pKa = 11.84 KK169 pKa = 7.5 RR170 pKa = 11.84 QVFGRR175 pKa = 11.84 MQRR178 pKa = 11.84 ARR180 pKa = 11.84 LYY182 pKa = 11.22 LEE184 pKa = 4.89 GNSHH188 pKa = 5.53 RR189 pKa = 11.84 VVRR192 pKa = 11.84 IGEE195 pKa = 4.15 LAEE198 pKa = 4.01 LTNFSSWYY206 pKa = 9.37 LSKK209 pKa = 9.81 TFQSLYY215 pKa = 10.41 EE216 pKa = 4.23 EE217 pKa = 4.85 SPQALSARR225 pKa = 11.84 LRR227 pKa = 11.84 LEE229 pKa = 3.77 RR230 pKa = 11.84 AADD233 pKa = 3.99 LLRR236 pKa = 11.84 DD237 pKa = 3.51 TDD239 pKa = 3.69 MMIGEE244 pKa = 4.45 VASASGFDD252 pKa = 3.65 NCCSFARR259 pKa = 11.84 AFRR262 pKa = 11.84 ARR264 pKa = 11.84 FGTSASHH271 pKa = 5.57 YY272 pKa = 9.39 RR273 pKa = 11.84 EE274 pKa = 4.19 SAAKK278 pKa = 10.43 LSPHH282 pKa = 5.57 SAKK285 pKa = 10.62 SLAASRR291 pKa = 11.84 KK292 pKa = 8.94 PRR294 pKa = 11.84 IATQSS299 pKa = 3.03
Molecular weight: 33.22 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.355
IPC2_protein 9.472
IPC_protein 10.379
Toseland 10.584
ProMoST 10.35
Dawson 10.672
Bjellqvist 10.438
Wikipedia 10.921
Rodwell 10.745
Grimsley 10.73
Solomon 10.818
Lehninger 10.789
Nozaki 10.599
DTASelect 10.423
Thurlkill 10.584
EMBOSS 10.994
Sillero 10.628
Patrickios 10.438
IPC_peptide 10.833
IPC2_peptide 9.75
IPC2.peptide.svr19 8.618
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3376
0
3376
1140280
29
5071
337.8
36.56
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.109 ± 0.067
0.765 ± 0.013
5.834 ± 0.031
5.033 ± 0.04
3.297 ± 0.026
8.691 ± 0.046
2.205 ± 0.025
4.035 ± 0.031
2.562 ± 0.033
11.039 ± 0.059
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.185 ± 0.02
2.576 ± 0.038
5.286 ± 0.034
4.325 ± 0.033
7.329 ± 0.055
5.417 ± 0.037
4.943 ± 0.041
7.596 ± 0.043
1.539 ± 0.024
2.233 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here