Grass carp reovirus

Taxonomy: Viruses; Riboviria; Orthornavirae; Duplornaviricota; Resentoviricetes; Reovirales; Reoviridae; Spinareovirinae; Aquareovirus; Aquareovirus C

Average proteome isoelectric point is 6.21

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 11 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|E7DDK9|E7DDK9_9REOV Non-structural protein OS=Grass carp reovirus OX=128987 GN=NS2 PE=4 SV=1
MM1 pKa = 7.37ATRR4 pKa = 11.84DD5 pKa = 3.35SRR7 pKa = 11.84MTTEE11 pKa = 4.56HH12 pKa = 6.66VIALILAYY20 pKa = 9.68STTLDD25 pKa = 3.19EE26 pKa = 4.9AKK28 pKa = 10.02IHH30 pKa = 6.25EE31 pKa = 5.49EE32 pKa = 3.53ITNLQSAVEE41 pKa = 3.95QLTVRR46 pKa = 11.84LTEE49 pKa = 3.86LTNAVTSLDD58 pKa = 3.4ARR60 pKa = 11.84LTQVEE65 pKa = 4.26QQASKK70 pKa = 11.08LDD72 pKa = 3.85DD73 pKa = 4.67KK74 pKa = 11.4IGILSNRR81 pKa = 11.84IGANTASINFLAGIVDD97 pKa = 4.06ATRR100 pKa = 11.84IDD102 pKa = 4.15AVDD105 pKa = 3.7APLYY109 pKa = 10.36IYY111 pKa = 10.38IVDD114 pKa = 3.74DD115 pKa = 3.55QRR117 pKa = 11.84RR118 pKa = 11.84LAIATGDD125 pKa = 3.62GLFVKK130 pKa = 10.19DD131 pKa = 4.43RR132 pKa = 11.84KK133 pKa = 10.75LNGYY137 pKa = 7.27DD138 pKa = 3.29VRR140 pKa = 11.84SFPPIAVAKK149 pKa = 10.34YY150 pKa = 10.17NDD152 pKa = 3.58ILSFSLSSAPPLDD165 pKa = 3.55IVDD168 pKa = 4.01GKK170 pKa = 10.89LAVSTTSRR178 pKa = 11.84LFITSGKK185 pKa = 10.06LDD187 pKa = 3.55TNSYY191 pKa = 9.64VGSSSVDD198 pKa = 2.95ISGAAAEE205 pKa = 4.42KK206 pKa = 8.81TVSVRR211 pKa = 11.84TINPIIAGQDD221 pKa = 3.54GLSLSLSGVLEE232 pKa = 4.3VGSGLLLSGRR242 pKa = 11.84SWPLTLDD249 pKa = 3.62LRR251 pKa = 11.84VSTPLAYY258 pKa = 10.41DD259 pKa = 3.18HH260 pKa = 6.88TGVLSVVTRR269 pKa = 11.84YY270 pKa = 9.72PLDD273 pKa = 3.46VTSAGMGVNMQVPLRR288 pKa = 11.84LHH290 pKa = 6.53GVDD293 pKa = 5.51LGLAYY298 pKa = 8.58NTQDD302 pKa = 3.21FSIVDD307 pKa = 4.4GYY309 pKa = 10.16LTLNRR314 pKa = 11.84SQGKK318 pKa = 9.84LEE320 pKa = 4.04EE321 pKa = 4.61LGEE324 pKa = 4.31AVNMNATLVDD334 pKa = 4.55LNDD337 pKa = 4.35GGLQALEE344 pKa = 4.3TDD346 pKa = 4.33LSLEE350 pKa = 4.17KK351 pKa = 10.21PLNAMQCLFEE361 pKa = 6.06HH362 pKa = 6.77EE363 pKa = 4.71GTWRR367 pKa = 11.84WKK369 pKa = 10.18FGVRR373 pKa = 11.84MSTEE377 pKa = 3.69PTITTVRR384 pKa = 11.84VNVHH388 pKa = 5.32STWSVAVQEE397 pKa = 5.02SIAICAITTTTAGAGRR413 pKa = 11.84GQGPRR418 pKa = 11.84VTIPFRR424 pKa = 11.84RR425 pKa = 11.84ADD427 pKa = 3.57LTDD430 pKa = 3.4DD431 pKa = 3.45MMRR434 pKa = 11.84AGFDD438 pKa = 3.25VWKK441 pKa = 10.22SDD443 pKa = 3.91YY444 pKa = 10.42IRR446 pKa = 11.84EE447 pKa = 4.02EE448 pKa = 4.14AFVGTLRR455 pKa = 11.84GLYY458 pKa = 9.82RR459 pKa = 11.84RR460 pKa = 11.84WSDD463 pKa = 2.83KK464 pKa = 10.88HH465 pKa = 7.63LLAEE469 pKa = 5.35FYY471 pKa = 11.23AHH473 pKa = 6.35GRR475 pKa = 11.84LYY477 pKa = 9.38PTEE480 pKa = 4.84DD481 pKa = 4.49DD482 pKa = 3.83IEE484 pKa = 4.52LVLTPRR490 pKa = 11.84STDD493 pKa = 4.95DD494 pKa = 3.44EE495 pKa = 4.89YY496 pKa = 11.65EE497 pKa = 3.96WAGLRR502 pKa = 11.84NVHH505 pKa = 5.2WTVVCCKK512 pKa = 10.53

Molecular weight: 56.04 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|E7DDL0|E7DDL0_9REOV Reovirus virion structural protein OS=Grass carp reovirus OX=128987 GN=VP6 PE=4 SV=1
MM1 pKa = 7.34YY2 pKa = 10.44LEE4 pKa = 4.99LFIAVMWHH12 pKa = 6.33FMLAFCAYY20 pKa = 7.91VWYY23 pKa = 10.07IHH25 pKa = 6.71GPTLRR30 pKa = 11.84RR31 pKa = 11.84LYY33 pKa = 10.58QRR35 pKa = 11.84LTATTANAICQTDD48 pKa = 3.38STYY51 pKa = 11.28VCTATVSTMTDD62 pKa = 3.21DD63 pKa = 3.63LTNASSSASTSSTASSATTSFVALPRR89 pKa = 11.84LSLDD93 pKa = 3.76DD94 pKa = 5.08LKK96 pKa = 10.3THH98 pKa = 6.96SKK100 pKa = 10.14FRR102 pKa = 11.84WLWFSNNPLTIDD114 pKa = 4.19LSTDD118 pKa = 3.05EE119 pKa = 4.83LTTPFNSPQLHH130 pKa = 6.76DD131 pKa = 3.94VLFMPQYY138 pKa = 9.93TLAAYY143 pKa = 8.75PYY145 pKa = 7.7MHH147 pKa = 7.43RR148 pKa = 11.84SLHH151 pKa = 5.41LALYY155 pKa = 10.05FRR157 pKa = 11.84FGYY160 pKa = 9.58NMRR163 pKa = 11.84YY164 pKa = 9.64LLRR167 pKa = 11.84EE168 pKa = 3.98HH169 pKa = 7.1PGIPPLRR176 pKa = 11.84VTCPSMRR183 pKa = 11.84VLFMDD188 pKa = 3.72KK189 pKa = 10.22QRR191 pKa = 11.84NKK193 pKa = 10.7LEE195 pKa = 4.17EE196 pKa = 4.39PVCSICRR203 pKa = 11.84LWIVDD208 pKa = 4.19GPQLPIGNCGRR219 pKa = 11.84KK220 pKa = 9.6YY221 pKa = 10.11KK222 pKa = 10.33VHH224 pKa = 6.96EE225 pKa = 4.15IFNAFSLEE233 pKa = 3.72PHH235 pKa = 6.81HH236 pKa = 6.82IVNAFDD242 pKa = 4.0VIPIISLLKK251 pKa = 10.42CLQLDD256 pKa = 3.57ISMLAGLSLTALTQLSEE273 pKa = 4.18LSRR276 pKa = 11.84DD277 pKa = 3.61PDD279 pKa = 3.47VTPAGFLAAIQFDD292 pKa = 4.28GHH294 pKa = 6.4TSTVDD299 pKa = 3.15CRR301 pKa = 11.84GDD303 pKa = 3.1RR304 pKa = 11.84LYY306 pKa = 11.09SHH308 pKa = 7.48PMLLILFALFCYY320 pKa = 9.99RR321 pKa = 11.84RR322 pKa = 11.84RR323 pKa = 11.84SRR325 pKa = 11.84VYY327 pKa = 9.98FRR329 pKa = 11.84EE330 pKa = 4.27CYY332 pKa = 10.06LRR334 pKa = 11.84DD335 pKa = 3.55YY336 pKa = 11.19RR337 pKa = 11.84RR338 pKa = 11.84LTSSLPDD345 pKa = 3.21MVPYY349 pKa = 10.74SRR351 pKa = 11.84TPGKK355 pKa = 10.49AKK357 pKa = 10.51AKK359 pKa = 10.56SPP361 pKa = 3.64

Molecular weight: 41.31 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

11

0

11

7837

310

1294

712.5

79.02

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.332 ± 0.434

1.544 ± 0.148

6.278 ± 0.234

3.879 ± 0.273

3.535 ± 0.238

6.087 ± 0.489

2.131 ± 0.262

5.627 ± 0.278

3.241 ± 0.172

10.08 ± 0.433

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.871 ± 0.155

3.828 ± 0.255

5.678 ± 0.253

3.33 ± 0.281

6.24 ± 0.39

7.962 ± 0.468

7.324 ± 0.586

6.929 ± 0.441

1.799 ± 0.19

3.305 ± 0.232
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski