Anaerocolumna jejuensis DSM 15929

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Lachnospiraceae; Anaerocolumna; Anaerocolumna jejuensis

Average proteome isoelectric point is 6.2

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5667 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1M6WG66|A0A1M6WG66_9FIRM Beta-galactosidase (Fragment) OS=Anaerocolumna jejuensis DSM 15929 OX=1121322 GN=SAMN02745136_03658 PE=4 SV=1
MM1 pKa = 7.98SEE3 pKa = 4.06FLKK6 pKa = 11.25LEE8 pKa = 4.17DD9 pKa = 5.22FIEE12 pKa = 4.8LSDD15 pKa = 3.66SGYY18 pKa = 11.17EE19 pKa = 3.79EE20 pKa = 6.2AIVQFLQNNGITVDD34 pKa = 3.36DD35 pKa = 4.1FEE37 pKa = 5.26YY38 pKa = 10.93VGKK41 pKa = 10.36CDD43 pKa = 4.13SEE45 pKa = 4.76CPEE48 pKa = 4.71CEE50 pKa = 4.94AEE52 pKa = 5.24SFWVWRR58 pKa = 11.84RR59 pKa = 11.84DD60 pKa = 3.26LGFIEE65 pKa = 5.12DD66 pKa = 3.85GKK68 pKa = 11.01IFTFDD73 pKa = 4.73LDD75 pKa = 4.1TADD78 pKa = 4.14DD79 pKa = 3.96TLDD82 pKa = 3.49FAIYY86 pKa = 10.53LCDD89 pKa = 4.19KK90 pKa = 9.47CGKK93 pKa = 5.68WTTYY97 pKa = 10.39IEE99 pKa = 4.18

Molecular weight: 11.54 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1M6XWM6|A0A1M6XWM6_9FIRM Uncharacterized protein OS=Anaerocolumna jejuensis DSM 15929 OX=1121322 GN=SAMN02745136_04095 PE=4 SV=1
MM1 pKa = 7.82AKK3 pKa = 9.58TSMKK7 pKa = 10.05IKK9 pKa = 9.13QQRR12 pKa = 11.84PQKK15 pKa = 10.52FSTRR19 pKa = 11.84EE20 pKa = 3.66YY21 pKa = 10.2NRR23 pKa = 11.84CRR25 pKa = 11.84ICGRR29 pKa = 11.84PHH31 pKa = 6.8GYY33 pKa = 9.03LRR35 pKa = 11.84KK36 pKa = 10.07YY37 pKa = 9.32GICRR41 pKa = 11.84ICFRR45 pKa = 11.84EE46 pKa = 3.97LAYY49 pKa = 9.84KK50 pKa = 10.19GQIPGVKK57 pKa = 9.25KK58 pKa = 10.9ASWW61 pKa = 3.03

Molecular weight: 7.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5667

0

5667

1869037

39

2864

329.8

37.12

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.886 ± 0.034

1.261 ± 0.013

5.369 ± 0.022

7.303 ± 0.039

4.322 ± 0.023

6.876 ± 0.035

1.582 ± 0.013

7.959 ± 0.033

7.451 ± 0.03

9.446 ± 0.033

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.771 ± 0.018

5.043 ± 0.029

3.198 ± 0.019

3.005 ± 0.016

3.926 ± 0.025

6.368 ± 0.028

5.489 ± 0.034

6.218 ± 0.023

0.993 ± 0.011

4.531 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski