Amino acid dipepetide frequency for Anaerocolumna jejuensis DSM 15929

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	6.78AlaAla: 6.78 ± 0.087	0.896AlaCys: 0.896 ± 0.023	3.899AlaAsp: 3.899 ± 0.054	4.764AlaGlu: 4.764 ± 0.055	3.052AlaPhe: 3.052 ± 0.04	5.751AlaGly: 5.751 ± 0.072	0.907AlaHis: 0.907 ± 0.024	4.944AlaIle: 4.944 ± 0.061	4.503AlaLys: 4.503 ± 0.058	6.648AlaLeu: 6.648 ± 0.064	1.948AlaMet: 1.948 ± 0.03	2.545AlaAsn: 2.545 ± 0.038	1.864AlaPro: 1.864 ± 0.04	1.795AlaGln: 1.795 ± 0.026	2.564AlaArg: 2.564 ± 0.044	4.026AlaSer: 4.026 ± 0.056	2.853AlaThr: 2.853 ± 0.05	5.665AlaVal: 5.665 ± 0.06	0.64AlaTrp: 0.64 ± 0.018	2.816AlaTyr: 2.816 ± 0.042	0.0AlaXaa: 0.0 ± 0.0
Cys	0.721CysAla: 0.721 ± 0.021	0.215CysCys: 0.215 ± 0.011	0.702CysAsp: 0.702 ± 0.021	0.777CysGlu: 0.777 ± 0.022	0.593CysPhe: 0.593 ± 0.018	1.151CysGly: 1.151 ± 0.031	0.251CysHis: 0.251 ± 0.01	1.09CysIle: 1.09 ± 0.027	0.745CysLys: 0.745 ± 0.018	1.152CysLeu: 1.152 ± 0.026	0.333CysMet: 0.333 ± 0.015	0.572CysAsn: 0.572 ± 0.018	0.504CysPro: 0.504 ± 0.02	0.337CysGln: 0.337 ± 0.011	0.58CysArg: 0.58 ± 0.017	0.866CysSer: 0.866 ± 0.023	0.616CysThr: 0.616 ± 0.018	0.692CysVal: 0.692 ± 0.02	0.122CysTrp: 0.122 ± 0.008	0.59CysTyr: 0.59 ± 0.02	0.0CysXaa: 0.0 ± 0.0
Asp	3.232AspAla: 3.232 ± 0.051	0.692AspCys: 0.692 ± 0.02	2.387AspAsp: 2.387 ± 0.04	3.646AspGlu: 3.646 ± 0.04	2.86AspPhe: 2.86 ± 0.041	3.839AspGly: 3.839 ± 0.056	0.585AspHis: 0.585 ± 0.017	5.115AspIle: 5.115 ± 0.055	4.366AspLys: 4.366 ± 0.051	4.254AspLeu: 4.254 ± 0.049	1.645AspMet: 1.645 ± 0.029	2.834AspAsn: 2.834 ± 0.039	1.388AspPro: 1.388 ± 0.026	1.067AspGln: 1.067 ± 0.023	2.17AspArg: 2.17 ± 0.038	3.603AspSer: 3.603 ± 0.054	3.271AspThr: 3.271 ± 0.047	2.966AspVal: 2.966 ± 0.041	0.669AspTrp: 0.669 ± 0.02	3.097AspTyr: 3.097 ± 0.046	0.0AspXaa: 0.0 ± 0.0
Glu	5.336GluAla: 5.336 ± 0.065	0.738GluCys: 0.738 ± 0.021	4.006GluAsp: 4.006 ± 0.047	7.37GluGlu: 7.37 ± 0.088	2.736GluPhe: 2.736 ± 0.043	4.538GluGly: 4.538 ± 0.06	1.154GluHis: 1.154 ± 0.025	5.524GluIle: 5.524 ± 0.056	6.573GluLys: 6.573 ± 0.069	6.895GluLeu: 6.895 ± 0.069	1.964GluMet: 1.964 ± 0.038	4.213GluAsn: 4.213 ± 0.052	1.757GluPro: 1.757 ± 0.03	2.538GluGln: 2.538 ± 0.043	2.867GluArg: 2.867 ± 0.041	3.357GluSer: 3.357 ± 0.046	3.214GluThr: 3.214 ± 0.047	4.256GluVal: 4.256 ± 0.052	0.688GluTrp: 0.688 ± 0.019	3.307GluTyr: 3.307 ± 0.048	0.0GluXaa: 0.0 ± 0.0
Phe	2.674PheAla: 2.674 ± 0.035	0.635PheCys: 0.635 ± 0.02	2.524PheAsp: 2.524 ± 0.042	2.685PheGlu: 2.685 ± 0.04	1.966PhePhe: 1.966 ± 0.044	2.877PheGly: 2.877 ± 0.045	0.952PheHis: 0.952 ± 0.023	3.422PheIle: 3.422 ± 0.048	2.467PheLys: 2.467 ± 0.039	4.447PheLeu: 4.447 ± 0.06	1.156PheMet: 1.156 ± 0.024	1.981PheAsn: 1.981 ± 0.035	1.38PhePro: 1.38 ± 0.027	1.452PheGln: 1.452 ± 0.027	1.637PheArg: 1.637 ± 0.028	3.203PheSer: 3.203 ± 0.047	2.724PheThr: 2.724 ± 0.039	2.49PheVal: 2.49 ± 0.038	0.485PheTrp: 0.485 ± 0.016	2.061PheTyr: 2.061 ± 0.035	0.0PheXaa: 0.0 ± 0.0
Gly	4.401GlyAla: 4.401 ± 0.069	1.058GlyCys: 1.058 ± 0.026	3.297GlyAsp: 3.297 ± 0.046	4.54GlyGlu: 4.54 ± 0.061	3.259GlyPhe: 3.259 ± 0.038	4.587GlyGly: 4.587 ± 0.065	1.1GlyHis: 1.1 ± 0.027	6.57GlyIle: 6.57 ± 0.07	5.587GlyLys: 5.587 ± 0.062	5.967GlyLeu: 5.967 ± 0.073	2.156GlyMet: 2.156 ± 0.037	3.507GlyAsn: 3.507 ± 0.05	1.253GlyPro: 1.253 ± 0.035	1.847GlyGln: 1.847 ± 0.036	2.817GlyArg: 2.817 ± 0.039	4.317GlySer: 4.317 ± 0.056	4.068GlyThr: 4.068 ± 0.067	4.309GlyVal: 4.309 ± 0.047	0.72GlyTrp: 0.72 ± 0.02	3.406GlyTyr: 3.406 ± 0.049	0.0GlyXaa: 0.0 ± 0.0
His	0.857HisAla: 0.857 ± 0.022	0.249HisCys: 0.249 ± 0.013	0.78HisAsp: 0.78 ± 0.02	0.917HisGlu: 0.917 ± 0.021	0.856HisPhe: 0.856 ± 0.022	1.108HisGly: 1.108 ± 0.028	0.332HisHis: 0.332 ± 0.015	1.417HisIle: 1.417 ± 0.029	1.001HisLys: 1.001 ± 0.023	1.486HisLeu: 1.486 ± 0.031	0.463HisMet: 0.463 ± 0.016	0.806HisAsn: 0.806 ± 0.021	0.722HisPro: 0.722 ± 0.022	0.486HisGln: 0.486 ± 0.015	0.616HisArg: 0.616 ± 0.02	1.013HisSer: 1.013 ± 0.025	0.859HisThr: 0.859 ± 0.02	0.831HisVal: 0.831 ± 0.022	0.184HisTrp: 0.184 ± 0.011	0.835HisTyr: 0.835 ± 0.024	0.0HisXaa: 0.0 ± 0.0
Ile	5.502IleAla: 5.502 ± 0.055	1.159IleCys: 1.159 ± 0.027	4.129IleAsp: 4.129 ± 0.051	4.934IleGlu: 4.934 ± 0.056	3.151IlePhe: 3.151 ± 0.048	5.094IleGly: 5.094 ± 0.059	1.375IleHis: 1.375 ± 0.031	6.36IleIle: 6.36 ± 0.075	5.52IleLys: 5.52 ± 0.057	7.903IleLeu: 7.903 ± 0.083	2.084IleMet: 2.084 ± 0.038	3.956IleAsn: 3.956 ± 0.044	3.356IlePro: 3.356 ± 0.041	2.396IleGln: 2.396 ± 0.038	3.504IleArg: 3.504 ± 0.04	5.728IleSer: 5.728 ± 0.069	5.077IleThr: 5.077 ± 0.06	4.493IleVal: 4.493 ± 0.061	0.699IleTrp: 0.699 ± 0.02	3.176IleTyr: 3.176 ± 0.041	0.0IleXaa: 0.0 ± 0.0
Lys	5.567LysAla: 5.567 ± 0.062	0.6LysCys: 0.6 ± 0.02	4.708LysAsp: 4.708 ± 0.054	7.81LysGlu: 7.81 ± 0.083	2.065LysPhe: 2.065 ± 0.031	4.807LysGly: 4.807 ± 0.059	0.988LysHis: 0.988 ± 0.022	5.166LysIle: 5.166 ± 0.056	6.287LysLys: 6.287 ± 0.066	6.388LysLeu: 6.388 ± 0.057	2.043LysMet: 2.043 ± 0.032	4.312LysAsn: 4.312 ± 0.051	2.046LysPro: 2.046 ± 0.036	2.482LysGln: 2.482 ± 0.043	2.838LysArg: 2.838 ± 0.04	4.092LysSer: 4.092 ± 0.051	3.94LysThr: 3.94 ± 0.047	4.498LysVal: 4.498 ± 0.054	0.688LysTrp: 0.688 ± 0.023	3.189LysTyr: 3.189 ± 0.042	0.0LysXaa: 0.0 ± 0.0
Leu	6.342LeuAla: 6.342 ± 0.063	1.32LeuCys: 1.32 ± 0.027	4.74LeuAsp: 4.74 ± 0.049	6.229LeuGlu: 6.229 ± 0.07	4.222LeuPhe: 4.222 ± 0.062	5.772LeuGly: 5.772 ± 0.065	1.529LeuHis: 1.529 ± 0.031	6.908LeuIle: 6.908 ± 0.078	7.49LeuLys: 7.49 ± 0.064	10.004LeuLeu: 10.004 ± 0.116	2.499LeuMet: 2.499 ± 0.032	4.715LeuAsn: 4.715 ± 0.045	3.693LeuPro: 3.693 ± 0.039	2.754LeuGln: 2.754 ± 0.041	3.481LeuArg: 3.481 ± 0.046	7.188LeuSer: 7.188 ± 0.07	5.435LeuThr: 5.435 ± 0.059	5.301LeuVal: 5.301 ± 0.06	0.859LeuTrp: 0.859 ± 0.025	3.977LeuTyr: 3.977 ± 0.047	0.0LeuXaa: 0.0 ± 0.0
Met	2.101MetAla: 2.101 ± 0.035	0.239MetCys: 0.239 ± 0.012	1.677MetAsp: 1.677 ± 0.032	2.387MetGlu: 2.387 ± 0.044	0.942MetPhe: 0.942 ± 0.02	1.905MetGly: 1.905 ± 0.037	0.414MetHis: 0.414 ± 0.016	1.95MetIle: 1.95 ± 0.038	2.501MetLys: 2.501 ± 0.035	2.557MetLeu: 2.557 ± 0.039	0.73MetMet: 0.73 ± 0.019	1.542MetAsn: 1.542 ± 0.03	1.044MetPro: 1.044 ± 0.024	0.877MetGln: 0.877 ± 0.02	0.972MetArg: 0.972 ± 0.026	1.637MetSer: 1.637 ± 0.03	1.459MetThr: 1.459 ± 0.029	1.702MetVal: 1.702 ± 0.029	0.213MetTrp: 0.213 ± 0.01	0.858MetTyr: 0.858 ± 0.02	0.0MetXaa: 0.0 ± 0.0
Asn	3.106AsnAla: 3.106 ± 0.037	0.638AsnCys: 0.638 ± 0.021	2.316AsnAsp: 2.316 ± 0.039	3.052AsnGlu: 3.052 ± 0.045	1.951AsnPhe: 1.951 ± 0.031	3.765AsnGly: 3.765 ± 0.051	0.853AsnHis: 0.853 ± 0.022	4.288AsnIle: 4.288 ± 0.047	3.589AsnLys: 3.589 ± 0.045	4.53AsnLeu: 4.53 ± 0.049	1.401AsnMet: 1.401 ± 0.029	2.764AsnAsn: 2.764 ± 0.052	2.204AsnPro: 2.204 ± 0.037	1.724AsnGln: 1.724 ± 0.032	2.149AsnArg: 2.149 ± 0.033	3.303AsnSer: 3.303 ± 0.054	3.067AsnThr: 3.067 ± 0.052	2.761AsnVal: 2.761 ± 0.042	0.53AsnTrp: 0.53 ± 0.018	2.441AsnTyr: 2.441 ± 0.041	0.0AsnXaa: 0.0 ± 0.0
Pro	2.332ProAla: 2.332 ± 0.036	0.404ProCys: 0.404 ± 0.017	2.111ProAsp: 2.111 ± 0.033	2.76ProGlu: 2.76 ± 0.038	1.64ProPhe: 1.64 ± 0.032	2.24ProGly: 2.24 ± 0.037	0.53ProHis: 0.53 ± 0.019	2.238ProIle: 2.238 ± 0.036	1.914ProLys: 1.914 ± 0.035	2.896ProLeu: 2.896 ± 0.041	0.787ProMet: 0.787 ± 0.022	1.313ProAsn: 1.313 ± 0.028	0.697ProPro: 0.697 ± 0.023	0.911ProGln: 0.911 ± 0.025	0.927ProArg: 0.927 ± 0.022	2.012ProSer: 2.012 ± 0.038	1.569ProThr: 1.569 ± 0.055	2.815ProVal: 2.815 ± 0.047	0.377ProTrp: 0.377 ± 0.014	1.511ProTyr: 1.511 ± 0.033	0.0ProXaa: 0.0 ± 0.0
Gln	2.114GlnAla: 2.114 ± 0.036	0.306GlnCys: 0.306 ± 0.013	1.425GlnAsp: 1.425 ± 0.027	2.327GlnGlu: 2.327 ± 0.041	1.216GlnPhe: 1.216 ± 0.026	1.925GlnGly: 1.925 ± 0.034	0.375GlnHis: 0.375 ± 0.014	2.415GlnIle: 2.415 ± 0.042	2.557GlnLys: 2.557 ± 0.041	2.809GlnLeu: 2.809 ± 0.044	0.949GlnMet: 0.949 ± 0.021	1.664GlnAsn: 1.664 ± 0.029	0.822GlnPro: 0.822 ± 0.021	0.993GlnGln: 0.993 ± 0.026	1.211GlnArg: 1.211 ± 0.024	1.705GlnSer: 1.705 ± 0.035	1.588GlnThr: 1.588 ± 0.028	1.843GlnVal: 1.843 ± 0.031	0.333GlnTrp: 0.333 ± 0.013	1.478GlnTyr: 1.478 ± 0.026	0.0GlnXaa: 0.0 ± 0.0
Arg	2.225ArgAla: 2.225 ± 0.04	0.453ArgCys: 0.453 ± 0.017	1.95ArgAsp: 1.95 ± 0.04	3.461ArgGlu: 3.461 ± 0.052	1.736ArgPhe: 1.736 ± 0.032	2.18ArgGly: 2.18 ± 0.038	0.628ArgHis: 0.628 ± 0.018	3.537ArgIle: 3.537 ± 0.046	3.327ArgLys: 3.327 ± 0.048	3.675ArgLeu: 3.675 ± 0.046	1.263ArgMet: 1.263 ± 0.027	2.243ArgAsn: 2.243 ± 0.035	1.05ArgPro: 1.05 ± 0.024	1.353ArgGln: 1.353 ± 0.027	1.768ArgArg: 1.768 ± 0.036	1.92ArgSer: 1.92 ± 0.039	2.004ArgThr: 2.004 ± 0.033	2.29ArgVal: 2.29 ± 0.034	0.346ArgTrp: 0.346 ± 0.015	1.849ArgTyr: 1.849 ± 0.034	0.001ArgXaa: 0.001 ± 0.001
Ser	4.155SerAla: 4.155 ± 0.053	0.822SerCys: 0.822 ± 0.02	3.501SerAsp: 3.501 ± 0.042	3.997SerGlu: 3.997 ± 0.043	3.125SerPhe: 3.125 ± 0.047	5.157SerGly: 5.157 ± 0.062	1.057SerHis: 1.057 ± 0.025	5.058SerIle: 5.058 ± 0.061	4.158SerLys: 4.158 ± 0.056	6.307SerLeu: 6.307 ± 0.068	1.758SerMet: 1.758 ± 0.029	2.938SerAsn: 2.938 ± 0.049	1.898SerPro: 1.898 ± 0.033	1.893SerGln: 1.893 ± 0.039	2.56SerArg: 2.56 ± 0.038	4.375SerSer: 4.375 ± 0.07	3.181SerThr: 3.181 ± 0.052	4.086SerVal: 4.086 ± 0.047	0.655SerTrp: 0.655 ± 0.021	3.0SerTyr: 3.0 ± 0.047	0.0SerXaa: 0.0 ± 0.0
Thr	4.523ThrAla: 4.523 ± 0.07	0.551ThrCys: 0.551 ± 0.017	3.172ThrAsp: 3.172 ± 0.048	3.799ThrGlu: 3.799 ± 0.046	2.308ThrPhe: 2.308 ± 0.039	4.748ThrGly: 4.748 ± 0.067	0.753ThrHis: 0.753 ± 0.019	4.161ThrIle: 4.161 ± 0.053	3.365ThrLys: 3.365 ± 0.05	5.108ThrLeu: 5.108 ± 0.052	1.349ThrMet: 1.349 ± 0.029	2.325ThrAsn: 2.325 ± 0.042	2.165ThrPro: 2.165 ± 0.058	1.407ThrGln: 1.407 ± 0.028	1.838ThrArg: 1.838 ± 0.032	3.357ThrSer: 3.357 ± 0.054	2.757ThrThr: 2.757 ± 0.056	4.388ThrVal: 4.388 ± 0.061	0.499ThrTrp: 0.499 ± 0.02	2.32ThrTyr: 2.32 ± 0.043	0.001ThrXaa: 0.001 ± 0.001
Val	3.71ValAla: 3.71 ± 0.051	0.887ValCys: 0.887 ± 0.023	3.117ValAsp: 3.117 ± 0.044	3.746ValGlu: 3.746 ± 0.042	2.945ValPhe: 2.945 ± 0.037	3.488ValGly: 3.488 ± 0.047	0.919ValHis: 0.919 ± 0.021	5.328ValIle: 5.328 ± 0.053	4.8ValLys: 4.8 ± 0.067	6.232ValLeu: 6.232 ± 0.059	1.767ValMet: 1.767 ± 0.033	3.188ValAsn: 3.188 ± 0.04	2.263ValPro: 2.263 ± 0.038	1.652ValGln: 1.652 ± 0.031	2.392ValArg: 2.392 ± 0.036	4.428ValSer: 4.428 ± 0.048	4.176ValThr: 4.176 ± 0.057	3.82ValVal: 3.82 ± 0.048	0.625ValTrp: 0.625 ± 0.019	2.7ValTyr: 2.7 ± 0.041	0.0ValXaa: 0.0 ± 0.0
Trp	0.554TrpAla: 0.554 ± 0.017	0.143TrpCys: 0.143 ± 0.008	0.608TrpAsp: 0.608 ± 0.018	0.733TrpGlu: 0.733 ± 0.021	0.461TrpPhe: 0.461 ± 0.016	0.754TrpGly: 0.754 ± 0.024	0.2TrpHis: 0.2 ± 0.011	0.676TrpIle: 0.676 ± 0.02	0.761TrpLys: 0.761 ± 0.023	0.948TrpLeu: 0.948 ± 0.025	0.278TrpMet: 0.278 ± 0.011	0.645TrpAsn: 0.645 ± 0.018	0.261TrpPro: 0.261 ± 0.011	0.386TrpGln: 0.386 ± 0.017	0.34TrpArg: 0.34 ± 0.014	0.616TrpSer: 0.616 ± 0.019	0.449TrpThr: 0.449 ± 0.017	0.533TrpVal: 0.533 ± 0.018	0.14TrpTrp: 0.14 ± 0.01	0.445TrpTyr: 0.445 ± 0.017	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.628TyrAla: 2.628 ± 0.039	0.605TyrCys: 0.605 ± 0.021	2.639TyrAsp: 2.639 ± 0.049	3.095TyrGlu: 3.095 ± 0.043	2.135TyrPhe: 2.135 ± 0.034	3.077TyrGly: 3.077 ± 0.047	0.919TyrHis: 0.919 ± 0.022	3.426TyrIle: 3.426 ± 0.049	2.999TyrLys: 2.999 ± 0.047	4.255TyrLeu: 4.255 ± 0.05	1.136TyrMet: 1.136 ± 0.023	2.371TyrAsn: 2.371 ± 0.039	1.617TyrPro: 1.617 ± 0.031	1.703TyrGln: 1.703 ± 0.037	2.028TyrArg: 2.028 ± 0.033	2.934TyrSer: 2.934 ± 0.042	2.585TyrThr: 2.585 ± 0.045	2.445TyrVal: 2.445 ± 0.033	0.46TyrTrp: 0.46 ± 0.016	2.256TyrTyr: 2.256 ± 0.044	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.001XaaLys: 0.001 ± 0.001	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.001XaaTrp: 0.001 ± 0.001	0.0XaaTyr: 0.0 ± 0.0	0.003XaaXaa: 0.003 ± 0.002

Statistics based on 5667 proteins (1869037 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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