Planctomycetes bacterium Pan265

Taxonomy: cellular organisms; Bacteria; PVC group; Planctomycetes; unclassified Planctomycetes

Average proteome isoelectric point is 5.79

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2855 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A518C040|A0A518C040_9BACT Uncharacterized protein OS=Planctomycetes bacterium Pan265 OX=2527982 GN=Pan265_24570 PE=4 SV=1
MM1 pKa = 7.18TRR3 pKa = 11.84ITSGGVLASTLILALTTTTHH23 pKa = 6.33ADD25 pKa = 2.93IYY27 pKa = 10.68QWTASRR33 pKa = 11.84GIYY36 pKa = 10.05QSTTLTPDD44 pKa = 3.32GAGKK48 pKa = 8.04TAEE51 pKa = 4.88PYY53 pKa = 11.15ADD55 pKa = 3.51LQGLNLYY62 pKa = 9.7KK63 pKa = 10.55AYY65 pKa = 10.58LRR67 pKa = 11.84EE68 pKa = 4.25ADD70 pKa = 3.65LTQANLSNANLADD83 pKa = 4.15AYY85 pKa = 10.81LYY87 pKa = 11.01NATLTNTDD95 pKa = 3.92LSDD98 pKa = 5.43AIITEE103 pKa = 4.41ANFGSTTDD111 pKa = 3.92FGFTATQLYY120 pKa = 8.16STASYY125 pKa = 10.7KK126 pKa = 10.81NKK128 pKa = 10.0NLAGIKK134 pKa = 10.05LYY136 pKa = 11.34SNDD139 pKa = 3.19LTGWNLAGQNLASASFYY156 pKa = 11.23NATLTNTDD164 pKa = 3.92LSDD167 pKa = 5.43AIITEE172 pKa = 4.41ANFGSTTDD180 pKa = 3.27SGFTAAQLYY189 pKa = 7.97STASYY194 pKa = 10.7KK195 pKa = 10.81NKK197 pKa = 10.07NLAGIDD203 pKa = 3.65LRR205 pKa = 11.84YY206 pKa = 10.39NDD208 pKa = 3.64LTGWNLAGQNLTDD221 pKa = 5.37ADD223 pKa = 4.06FDD225 pKa = 4.76DD226 pKa = 5.98ANLTNTDD233 pKa = 4.45LNNADD238 pKa = 4.02LTDD241 pKa = 4.16ANLDD245 pKa = 3.77YY246 pKa = 11.77ANLTNTDD253 pKa = 3.72LSDD256 pKa = 5.43AIITEE261 pKa = 4.41ANFGSTTDD269 pKa = 3.18SGFTAGQLYY278 pKa = 8.26STASYY283 pKa = 10.7KK284 pKa = 10.81NKK286 pKa = 10.07NLAGIDD292 pKa = 3.65LRR294 pKa = 11.84YY295 pKa = 10.39NDD297 pKa = 3.57LTAWNLAGQNLTDD310 pKa = 4.59AYY312 pKa = 10.83FGLANLANTDD322 pKa = 3.93LSDD325 pKa = 4.43ALITGADD332 pKa = 4.58FYY334 pKa = 10.87RR335 pKa = 11.84TTDD338 pKa = 2.95SGFTAAQLYY347 pKa = 7.97STASYY352 pKa = 10.71KK353 pKa = 10.81NKK355 pKa = 10.13NLTGIDD361 pKa = 3.5LGYY364 pKa = 11.04NDD366 pKa = 3.71LTGWNLAGQNLTNTDD381 pKa = 3.87LNNANLTDD389 pKa = 3.91AYY391 pKa = 10.62LYY393 pKa = 10.98NATLTNTDD401 pKa = 3.92LSDD404 pKa = 5.43AIITEE409 pKa = 4.41ANFGSTTDD417 pKa = 3.24SGFTATQLYY426 pKa = 8.16STASYY431 pKa = 10.71KK432 pKa = 10.81NKK434 pKa = 10.06NLTGIKK440 pKa = 10.03LYY442 pKa = 11.31SNDD445 pKa = 3.19LTGWNLAGQNLTDD458 pKa = 3.75TDD460 pKa = 4.05LDD462 pKa = 4.14YY463 pKa = 11.89ANLTNTDD470 pKa = 4.0LNNANLTDD478 pKa = 4.27ANLDD482 pKa = 3.74YY483 pKa = 11.77ANLTNTDD490 pKa = 3.94LSDD493 pKa = 3.88ALITGADD500 pKa = 4.58FYY502 pKa = 10.87RR503 pKa = 11.84TTDD506 pKa = 2.91SGFTAGQLYY515 pKa = 8.26STASYY520 pKa = 10.7KK521 pKa = 10.81NKK523 pKa = 10.07NLAGIDD529 pKa = 3.56LGYY532 pKa = 11.05NDD534 pKa = 4.48LTDD537 pKa = 3.5WDD539 pKa = 4.6LSNLNLQSVVFNHH552 pKa = 6.94ANLHH556 pKa = 5.22NTDD559 pKa = 4.07FTASDD564 pKa = 3.89LRR566 pKa = 11.84RR567 pKa = 11.84SHH569 pKa = 6.58YY570 pKa = 10.97YY571 pKa = 9.49IDD573 pKa = 3.51RR574 pKa = 11.84TSGNRR579 pKa = 11.84MSQRR583 pKa = 11.84LAGVRR588 pKa = 11.84NAIHH592 pKa = 6.98PDD594 pKa = 3.09GRR596 pKa = 11.84VKK598 pKa = 10.93GLILQSSEE606 pKa = 4.03TLRR609 pKa = 11.84IWDD612 pKa = 4.86DD613 pKa = 3.61NPLDD617 pKa = 3.93EE618 pKa = 5.5SEE620 pKa = 4.1PAIPITIEE628 pKa = 3.66QEE630 pKa = 4.1LTIDD634 pKa = 3.57PAATLRR640 pKa = 11.84LVFEE644 pKa = 4.99DD645 pKa = 4.11NQWGSIIQFADD656 pKa = 3.29PDD658 pKa = 4.3TPVALAGTLLLEE670 pKa = 4.75FDD672 pKa = 5.5DD673 pKa = 5.96GLDD676 pKa = 3.71LQSLQITTYY685 pKa = 11.24QLFDD689 pKa = 3.46WTNADD694 pKa = 2.71ITGAFDD700 pKa = 3.94TILYY704 pKa = 8.72NGPGTFDD711 pKa = 3.36TSSLMATGEE720 pKa = 4.07VTYY723 pKa = 9.81FSATLLPADD732 pKa = 4.43ANLDD736 pKa = 3.52GSVDD740 pKa = 3.8MNDD743 pKa = 4.36LSILAANFGLPGVFGFTQGDD763 pKa = 3.93FNGDD767 pKa = 3.33NTVDD771 pKa = 4.61LLDD774 pKa = 4.22LSILASHH781 pKa = 7.57FGNTAIPEE789 pKa = 4.17PAAVTILLTLLPLSRR804 pKa = 11.84RR805 pKa = 11.84RR806 pKa = 11.84SAA808 pKa = 3.98

Molecular weight: 87.19 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A518BXZ9|A0A518BXZ9_9BACT Uncharacterized protein OS=Planctomycetes bacterium Pan265 OX=2527982 GN=Pan265_16960 PE=4 SV=1
MM1 pKa = 6.78STHH4 pKa = 4.96YY5 pKa = 9.85PKK7 pKa = 10.32RR8 pKa = 11.84RR9 pKa = 11.84SRR11 pKa = 11.84IKK13 pKa = 10.22RR14 pKa = 11.84SRR16 pKa = 11.84MWGFRR21 pKa = 11.84ARR23 pKa = 11.84MKK25 pKa = 9.78TKK27 pKa = 10.24QGRR30 pKa = 11.84KK31 pKa = 7.48MINRR35 pKa = 11.84KK36 pKa = 9.07RR37 pKa = 11.84RR38 pKa = 11.84VGRR41 pKa = 11.84SVNVRR46 pKa = 11.84HH47 pKa = 6.13NFF49 pKa = 3.25

Molecular weight: 6.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2855

0

2855

1052711

32

13493

368.7

40.34

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.529 ± 0.048

0.876 ± 0.021

6.715 ± 0.074

6.08 ± 0.057

3.327 ± 0.032

8.363 ± 0.062

2.294 ± 0.035

5.042 ± 0.04

2.556 ± 0.048

10.215 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.263 ± 0.036

3.047 ± 0.05

5.189 ± 0.042

3.497 ± 0.032

6.961 ± 0.08

5.516 ± 0.041

6.067 ± 0.08

7.497 ± 0.044

1.5 ± 0.026

2.466 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski