Paenibacillus curdlanolyticus YK9

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Paenibacillaceae; Paenibacillus; Paenibacillus curdlanolyticus

Average proteome isoelectric point is 6.31

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4803 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|E0IGP1|E0IGP1_9BACL Mutator family transposase (Fragment) OS=Paenibacillus curdlanolyticus YK9 OX=717606 GN=PaecuDRAFT_4836 PE=3 SV=1
MM1 pKa = 8.33VIMIVGCGSQDD12 pKa = 3.45PADD15 pKa = 3.89NDD17 pKa = 3.18LRR19 pKa = 11.84AAEE22 pKa = 3.96QPIVEE27 pKa = 4.36EE28 pKa = 4.46TSASSEE34 pKa = 4.19VVANEE39 pKa = 3.63SAEE42 pKa = 4.11PKK44 pKa = 8.78EE45 pKa = 4.03QEE47 pKa = 3.92IAEE50 pKa = 4.25TDD52 pKa = 3.48AVTVNYY58 pKa = 9.24AAQYY62 pKa = 8.05NGQWVTLIKK71 pKa = 10.32PEE73 pKa = 4.27RR74 pKa = 11.84CGTCKK79 pKa = 9.45NTLNIEE85 pKa = 3.83IDD87 pKa = 3.74SANQATVSIQQASDD101 pKa = 3.21HH102 pKa = 5.84GTHH105 pKa = 7.07LAGIDD110 pKa = 3.57FTVQLDD116 pKa = 4.27GQGSGAFTFDD126 pKa = 3.14EE127 pKa = 5.39DD128 pKa = 3.81GWFHH132 pKa = 5.99QGKK135 pKa = 8.24GTISLQSDD143 pKa = 4.13VIEE146 pKa = 4.21VTITEE151 pKa = 4.4MKK153 pKa = 10.53DD154 pKa = 3.18NPDD157 pKa = 3.33ADD159 pKa = 3.99EE160 pKa = 4.28VSNTYY165 pKa = 10.84NIFSGTQQFYY175 pKa = 10.11RR176 pKa = 11.84PEE178 pKa = 4.0VNRR181 pKa = 11.84RR182 pKa = 11.84AIMAKK187 pKa = 10.32LMEE190 pKa = 4.97DD191 pKa = 3.32TDD193 pKa = 3.63IDD195 pKa = 3.76ALEE198 pKa = 4.28FKK200 pKa = 11.28YY201 pKa = 10.66EE202 pKa = 3.97DD203 pKa = 3.56EE204 pKa = 4.12KK205 pKa = 11.61GYY207 pKa = 9.81WYY209 pKa = 10.5YY210 pKa = 11.11CAVIDD215 pKa = 4.52PNSEE219 pKa = 4.06GEE221 pKa = 4.09PLCYY225 pKa = 10.48GIDD228 pKa = 3.87PYY230 pKa = 11.02SGKK233 pKa = 10.38YY234 pKa = 10.03FDD236 pKa = 6.15DD237 pKa = 3.42ISGGYY242 pKa = 8.67EE243 pKa = 3.63GNIYY247 pKa = 10.17SDD249 pKa = 3.41HH250 pKa = 6.63SVPDD254 pKa = 3.89FDD256 pKa = 4.67EE257 pKa = 4.05VAAFVKK263 pKa = 10.56QKK265 pKa = 11.13LNLEE269 pKa = 4.3GDD271 pKa = 3.92TQFNGSDD278 pKa = 3.2YY279 pKa = 11.19GLYY282 pKa = 9.51IVQVGEE288 pKa = 4.01QEE290 pKa = 4.16FEE292 pKa = 4.13YY293 pKa = 11.03DD294 pKa = 3.93SDD296 pKa = 3.77TGDD299 pKa = 3.9LFDD302 pKa = 5.49SKK304 pKa = 7.67THH306 pKa = 6.46KK307 pKa = 10.89LLDD310 pKa = 4.71GIGLIEE316 pKa = 4.06PTQQ319 pKa = 3.3

Molecular weight: 35.28 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|E0IBI4|E0IBI4_9BACL MATE efflux family protein OS=Paenibacillus curdlanolyticus YK9 OX=717606 GN=PaecuDRAFT_3023 PE=4 SV=1
MM1 pKa = 8.05PKK3 pKa = 8.13TQKK6 pKa = 10.95ASFPVAQNAMTPLYY20 pKa = 10.45FRR22 pKa = 11.84ACVDD26 pKa = 4.26SIARR30 pKa = 11.84TAMSSLLHH38 pKa = 6.49AATPILARR46 pKa = 11.84HH47 pKa = 5.2TVLARR52 pKa = 11.84HH53 pKa = 6.13AVPSRR58 pKa = 11.84HH59 pKa = 5.94AVLARR64 pKa = 11.84HH65 pKa = 6.01AVLARR70 pKa = 11.84HH71 pKa = 5.97AVLARR76 pKa = 11.84HH77 pKa = 5.42TVLARR82 pKa = 11.84HH83 pKa = 6.15AVPARR88 pKa = 11.84HH89 pKa = 6.08AVLARR94 pKa = 11.84HH95 pKa = 6.09AVPSRR100 pKa = 11.84HH101 pKa = 5.94AVLARR106 pKa = 11.84HH107 pKa = 6.01AVLARR112 pKa = 11.84HH113 pKa = 6.01AVLARR118 pKa = 11.84HH119 pKa = 6.09AVPSRR124 pKa = 11.84HH125 pKa = 5.94AVLARR130 pKa = 11.84HH131 pKa = 6.15AVPARR136 pKa = 11.84HH137 pKa = 5.9AVPSRR142 pKa = 11.84RR143 pKa = 11.84AVLARR148 pKa = 11.84HH149 pKa = 5.87AVLARR154 pKa = 11.84HH155 pKa = 6.01AVLARR160 pKa = 11.84HH161 pKa = 6.01AVLARR166 pKa = 11.84HH167 pKa = 5.97AVLARR172 pKa = 11.84HH173 pKa = 5.56TVPARR178 pKa = 11.84HH179 pKa = 5.96AVLARR184 pKa = 11.84HH185 pKa = 6.01AVLARR190 pKa = 11.84HH191 pKa = 6.09AVPSRR196 pKa = 11.84HH197 pKa = 5.63AVPSRR202 pKa = 11.84RR203 pKa = 11.84AVLARR208 pKa = 11.84HH209 pKa = 5.87AVLARR214 pKa = 11.84HH215 pKa = 5.63TVPSRR220 pKa = 11.84HH221 pKa = 5.97AVLSLTSAIKK231 pKa = 10.23PSTARR236 pKa = 11.84AHH238 pKa = 6.41RR239 pKa = 11.84RR240 pKa = 11.84LPSAIASPTASAQNRR255 pKa = 11.84VSLSRR260 pKa = 11.84RR261 pKa = 11.84GFF263 pKa = 3.39

Molecular weight: 28.4 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4803

0

4803

1587121

30

3463

330.4

36.56

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.448 ± 0.051

0.766 ± 0.01

5.223 ± 0.024

6.388 ± 0.044

3.871 ± 0.025

7.589 ± 0.042

2.095 ± 0.019

6.274 ± 0.031

4.76 ± 0.035

9.8 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.72 ± 0.017

3.721 ± 0.034

4.073 ± 0.023

4.009 ± 0.024

5.387 ± 0.042

6.435 ± 0.033

5.602 ± 0.045

7.153 ± 0.031

1.296 ± 0.015

3.386 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski