Amino acid dipepetide frequency for Paenibacillus curdlanolyticus YK9

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	11.426AlaAla: 11.426 ± 0.156	0.811AlaCys: 0.811 ± 0.023	5.143AlaAsp: 5.143 ± 0.072	5.99AlaGlu: 5.99 ± 0.084	3.69AlaPhe: 3.69 ± 0.051	7.637AlaGly: 7.637 ± 0.099	1.638AlaHis: 1.638 ± 0.032	6.123AlaIle: 6.123 ± 0.073	4.33AlaLys: 4.33 ± 0.058	9.044AlaLeu: 9.044 ± 0.092	2.608AlaMet: 2.608 ± 0.041	3.28AlaAsn: 3.28 ± 0.051	3.237AlaPro: 3.237 ± 0.059	3.177AlaGln: 3.177 ± 0.047	4.124AlaArg: 4.124 ± 0.06	6.437AlaSer: 6.437 ± 0.084	4.497AlaThr: 4.497 ± 0.086	7.202AlaVal: 7.202 ± 0.087	1.069AlaTrp: 1.069 ± 0.03	3.017AlaTyr: 3.017 ± 0.047	0.001AlaXaa: 0.001 ± 0.001
Cys	0.629CysAla: 0.629 ± 0.022	0.112CysCys: 0.112 ± 0.009	0.405CysAsp: 0.405 ± 0.017	0.441CysGlu: 0.441 ± 0.016	0.302CysPhe: 0.302 ± 0.016	0.776CysGly: 0.776 ± 0.028	0.167CysHis: 0.167 ± 0.012	0.444CysIle: 0.444 ± 0.018	0.302CysLys: 0.302 ± 0.014	0.71CysLeu: 0.71 ± 0.026	0.226CysMet: 0.226 ± 0.014	0.261CysAsn: 0.261 ± 0.013	0.354CysPro: 0.354 ± 0.016	0.238CysGln: 0.238 ± 0.012	0.462CysArg: 0.462 ± 0.02	0.573CysSer: 0.573 ± 0.02	0.415CysThr: 0.415 ± 0.016	0.466CysVal: 0.466 ± 0.017	0.114CysTrp: 0.114 ± 0.008	0.266CysTyr: 0.266 ± 0.012	0.0CysXaa: 0.0 ± 0.0
Asp	4.918AspAla: 4.918 ± 0.073	0.396AspCys: 0.396 ± 0.015	2.534AspAsp: 2.534 ± 0.05	3.794AspGlu: 3.794 ± 0.062	1.978AspPhe: 1.978 ± 0.039	4.383AspGly: 4.383 ± 0.067	1.11AspHis: 1.11 ± 0.026	3.217AspIle: 3.217 ± 0.054	2.569AspLys: 2.569 ± 0.053	4.629AspLeu: 4.629 ± 0.063	1.309AspMet: 1.309 ± 0.032	1.867AspAsn: 1.867 ± 0.043	2.305AspPro: 2.305 ± 0.038	1.974AspGln: 1.974 ± 0.035	3.167AspArg: 3.167 ± 0.054	2.913AspSer: 2.913 ± 0.048	2.551AspThr: 2.551 ± 0.046	3.666AspVal: 3.666 ± 0.047	0.902AspTrp: 0.902 ± 0.028	2.045AspTyr: 2.045 ± 0.041	0.001AspXaa: 0.001 ± 0.001
Glu	6.683GluAla: 6.683 ± 0.089	0.376GluCys: 0.376 ± 0.017	2.825GluAsp: 2.825 ± 0.047	4.618GluGlu: 4.618 ± 0.09	1.786GluPhe: 1.786 ± 0.033	4.275GluGly: 4.275 ± 0.058	1.534GluHis: 1.534 ± 0.035	3.559GluIle: 3.559 ± 0.059	3.134GluLys: 3.134 ± 0.05	7.093GluLeu: 7.093 ± 0.094	1.67GluMet: 1.67 ± 0.034	1.974GluAsn: 1.974 ± 0.036	2.343GluPro: 2.343 ± 0.044	4.069GluGln: 4.069 ± 0.074	4.497GluArg: 4.497 ± 0.074	3.464GluSer: 3.464 ± 0.055	3.392GluThr: 3.392 ± 0.053	3.985GluVal: 3.985 ± 0.06	0.925GluTrp: 0.925 ± 0.026	1.684GluTyr: 1.684 ± 0.034	0.0GluXaa: 0.0 ± 0.0
Phe	3.461PheAla: 3.461 ± 0.051	0.336PheCys: 0.336 ± 0.015	2.373PheAsp: 2.373 ± 0.043	2.417PheGlu: 2.417 ± 0.034	1.469PhePhe: 1.469 ± 0.037	3.223PheGly: 3.223 ± 0.047	0.897PheHis: 0.897 ± 0.027	2.535PheIle: 2.535 ± 0.044	1.697PheLys: 1.697 ± 0.032	3.238PheLeu: 3.238 ± 0.056	1.055PheMet: 1.055 ± 0.028	1.568PheAsn: 1.568 ± 0.034	1.521PhePro: 1.521 ± 0.042	1.316PheGln: 1.316 ± 0.03	2.003PheArg: 2.003 ± 0.039	2.516PheSer: 2.516 ± 0.05	2.322PheThr: 2.322 ± 0.042	2.968PheVal: 2.968 ± 0.052	0.443PheTrp: 0.443 ± 0.019	1.355PheTyr: 1.355 ± 0.03	0.0PheXaa: 0.0 ± 0.0
Gly	6.701GlyAla: 6.701 ± 0.118	0.73GlyCys: 0.73 ± 0.022	3.77GlyAsp: 3.77 ± 0.051	4.516GlyGlu: 4.516 ± 0.056	3.142GlyPhe: 3.142 ± 0.051	5.96GlyGly: 5.96 ± 0.114	1.505GlyHis: 1.505 ± 0.036	5.486GlyIle: 5.486 ± 0.074	4.139GlyLys: 4.139 ± 0.053	6.979GlyLeu: 6.979 ± 0.076	2.324GlyMet: 2.324 ± 0.047	2.723GlyAsn: 2.723 ± 0.077	2.024GlyPro: 2.024 ± 0.048	2.917GlyGln: 2.917 ± 0.054	3.8GlyArg: 3.8 ± 0.057	5.03GlySer: 5.03 ± 0.084	4.744GlyThr: 4.744 ± 0.093	5.387GlyVal: 5.387 ± 0.063	1.155GlyTrp: 1.155 ± 0.037	2.859GlyTyr: 2.859 ± 0.048	0.0GlyXaa: 0.0 ± 0.0
His	2.014HisAla: 2.014 ± 0.046	0.215HisCys: 0.215 ± 0.013	1.045HisAsp: 1.045 ± 0.027	1.301HisGlu: 1.301 ± 0.031	0.89HisPhe: 0.89 ± 0.029	1.54HisGly: 1.54 ± 0.035	0.605HisHis: 0.605 ± 0.022	1.243HisIle: 1.243 ± 0.031	0.782HisLys: 0.782 ± 0.024	1.93HisLeu: 1.93 ± 0.04	0.568HisMet: 0.568 ± 0.02	0.67HisAsn: 0.67 ± 0.023	1.171HisPro: 1.171 ± 0.029	0.838HisGln: 0.838 ± 0.027	1.17HisArg: 1.17 ± 0.03	1.222HisSer: 1.222 ± 0.028	1.067HisThr: 1.067 ± 0.027	1.47HisVal: 1.47 ± 0.03	0.308HisTrp: 0.308 ± 0.015	0.901HisTyr: 0.901 ± 0.024	0.0HisXaa: 0.0 ± 0.0
Ile	6.546IleAla: 6.546 ± 0.071	0.522IleCys: 0.522 ± 0.018	3.788IleAsp: 3.788 ± 0.053	4.155IleGlu: 4.155 ± 0.07	1.915IlePhe: 1.915 ± 0.039	5.511IleGly: 5.511 ± 0.07	1.344IleHis: 1.344 ± 0.028	3.7IleIle: 3.7 ± 0.057	2.619IleLys: 2.619 ± 0.046	4.612IleLeu: 4.612 ± 0.069	1.501IleMet: 1.501 ± 0.031	2.263IleAsn: 2.263 ± 0.039	2.728IlePro: 2.728 ± 0.055	2.287IleGln: 2.287 ± 0.04	3.789IleArg: 3.789 ± 0.066	3.811IleSer: 3.811 ± 0.052	3.592IleThr: 3.592 ± 0.062	5.517IleVal: 5.517 ± 0.066	0.665IleTrp: 0.665 ± 0.021	1.876IleTyr: 1.876 ± 0.033	0.001IleXaa: 0.001 ± 0.001
Lys	4.285LysAla: 4.285 ± 0.056	0.227LysCys: 0.227 ± 0.014	2.449LysAsp: 2.449 ± 0.052	3.297LysGlu: 3.297 ± 0.052	1.4LysPhe: 1.4 ± 0.03	3.162LysGly: 3.162 ± 0.046	1.078LysHis: 1.078 ± 0.028	2.405LysIle: 2.405 ± 0.046	2.521LysLys: 2.521 ± 0.051	5.487LysLeu: 5.487 ± 0.077	1.298LysMet: 1.298 ± 0.031	1.559LysAsn: 1.559 ± 0.035	2.333LysPro: 2.333 ± 0.045	2.525LysGln: 2.525 ± 0.046	2.985LysArg: 2.985 ± 0.052	2.74LysSer: 2.74 ± 0.042	2.577LysThr: 2.577 ± 0.043	3.143LysVal: 3.143 ± 0.05	0.644LysTrp: 0.644 ± 0.019	1.489LysTyr: 1.489 ± 0.033	0.001LysXaa: 0.001 ± 0.001
Leu	9.18LeuAla: 9.18 ± 0.108	0.804LeuCys: 0.804 ± 0.025	5.114LeuAsp: 5.114 ± 0.062	5.851LeuGlu: 5.851 ± 0.089	4.341LeuPhe: 4.341 ± 0.07	6.635LeuGly: 6.635 ± 0.064	2.208LeuHis: 2.208 ± 0.044	5.856LeuIle: 5.856 ± 0.075	4.532LeuLys: 4.532 ± 0.062	10.74LeuLeu: 10.74 ± 0.136	2.517LeuMet: 2.517 ± 0.049	3.541LeuAsn: 3.541 ± 0.053	4.438LeuPro: 4.438 ± 0.066	4.128LeuGln: 4.128 ± 0.062	5.189LeuArg: 5.189 ± 0.079	6.834LeuSer: 6.834 ± 0.074	5.741LeuThr: 5.741 ± 0.069	6.344LeuVal: 6.344 ± 0.073	1.002LeuTrp: 1.002 ± 0.033	3.003LeuTyr: 3.003 ± 0.047	0.0LeuXaa: 0.0 ± 0.0
Met	2.334MetAla: 2.334 ± 0.042	0.161MetCys: 0.161 ± 0.011	1.37MetAsp: 1.37 ± 0.035	1.694MetGlu: 1.694 ± 0.031	0.986MetPhe: 0.986 ± 0.027	1.579MetGly: 1.579 ± 0.036	0.531MetHis: 0.531 ± 0.018	1.694MetIle: 1.694 ± 0.036	1.749MetLys: 1.749 ± 0.032	2.948MetLeu: 2.948 ± 0.052	0.862MetMet: 0.862 ± 0.026	1.387MetAsn: 1.387 ± 0.03	1.26MetPro: 1.26 ± 0.033	1.079MetGln: 1.079 ± 0.027	1.497MetArg: 1.497 ± 0.036	1.849MetSer: 1.849 ± 0.034	1.707MetThr: 1.707 ± 0.034	1.542MetVal: 1.542 ± 0.037	0.246MetTrp: 0.246 ± 0.012	0.726MetTyr: 0.726 ± 0.022	0.0MetXaa: 0.0 ± 0.0
Asn	3.377AsnAla: 3.377 ± 0.056	0.246AsnCys: 0.246 ± 0.013	2.007AsnAsp: 2.007 ± 0.043	2.486AsnGlu: 2.486 ± 0.047	1.184AsnPhe: 1.184 ± 0.034	3.64AsnGly: 3.64 ± 0.093	0.772AsnHis: 0.772 ± 0.021	2.056AsnIle: 2.056 ± 0.036	1.948AsnLys: 1.948 ± 0.036	2.989AsnLeu: 2.989 ± 0.047	0.916AsnMet: 0.916 ± 0.026	1.573AsnAsn: 1.573 ± 0.047	1.934AsnPro: 1.934 ± 0.04	1.436AsnGln: 1.436 ± 0.035	2.225AsnArg: 2.225 ± 0.041	1.905AsnSer: 1.905 ± 0.042	1.888AsnThr: 1.888 ± 0.05	2.84AsnVal: 2.84 ± 0.056	0.554AsnTrp: 0.554 ± 0.02	1.234AsnTyr: 1.234 ± 0.03	0.0AsnXaa: 0.0 ± 0.0
Pro	3.939ProAla: 3.939 ± 0.077	0.268ProCys: 0.268 ± 0.014	2.532ProAsp: 2.532 ± 0.042	2.929ProGlu: 2.929 ± 0.06	1.863ProPhe: 1.863 ± 0.043	2.75ProGly: 2.75 ± 0.055	0.826ProHis: 0.826 ± 0.026	2.706ProIle: 2.706 ± 0.047	1.624ProLys: 1.624 ± 0.027	3.964ProLeu: 3.964 ± 0.061	0.988ProMet: 0.988 ± 0.027	1.71ProAsn: 1.71 ± 0.035	1.384ProPro: 1.384 ± 0.047	1.379ProGln: 1.379 ± 0.034	1.54ProArg: 1.54 ± 0.035	2.908ProSer: 2.908 ± 0.044	2.204ProThr: 2.204 ± 0.047	3.165ProVal: 3.165 ± 0.053	0.478ProTrp: 0.478 ± 0.017	1.578ProTyr: 1.578 ± 0.039	0.001ProXaa: 0.001 ± 0.001
Gln	4.063GlnAla: 4.063 ± 0.064	0.231GlnCys: 0.231 ± 0.014	1.574GlnAsp: 1.574 ± 0.04	2.386GlnGlu: 2.386 ± 0.044	1.619GlnPhe: 1.619 ± 0.032	2.74GlnGly: 2.74 ± 0.054	0.911GlnHis: 0.911 ± 0.026	2.31GlnIle: 2.31 ± 0.042	1.558GlnLys: 1.558 ± 0.03	4.877GlnLeu: 4.877 ± 0.079	1.096GlnMet: 1.096 ± 0.028	1.173GlnAsn: 1.173 ± 0.032	1.793GlnPro: 1.793 ± 0.037	2.309GlnGln: 2.309 ± 0.052	2.152GlnArg: 2.152 ± 0.035	2.628GlnSer: 2.628 ± 0.046	2.166GlnThr: 2.166 ± 0.044	2.479GlnVal: 2.479 ± 0.037	0.687GlnTrp: 0.687 ± 0.022	1.339GlnTyr: 1.339 ± 0.03	0.0GlnXaa: 0.0 ± 0.0
Arg	4.089ArgAla: 4.089 ± 0.064	0.395ArgCys: 0.395 ± 0.016	2.595ArgAsp: 2.595 ± 0.046	3.716ArgGlu: 3.716 ± 0.069	2.328ArgPhe: 2.328 ± 0.043	3.291ArgGly: 3.291 ± 0.057	1.154ArgHis: 1.154 ± 0.036	3.836ArgIle: 3.836 ± 0.05	2.907ArgLys: 2.907 ± 0.054	5.679ArgLeu: 5.679 ± 0.086	1.876ArgMet: 1.876 ± 0.038	2.117ArgAsn: 2.117 ± 0.039	1.903ArgPro: 1.903 ± 0.038	2.19ArgGln: 2.19 ± 0.045	3.014ArgArg: 3.014 ± 0.066	3.46ArgSer: 3.46 ± 0.054	3.051ArgThr: 3.051 ± 0.047	3.429ArgVal: 3.429 ± 0.048	0.772ArgTrp: 0.772 ± 0.024	2.064ArgTyr: 2.064 ± 0.036	0.0ArgXaa: 0.0 ± 0.0
Ser	5.674SerAla: 5.674 ± 0.076	0.464SerCys: 0.464 ± 0.018	3.319SerAsp: 3.319 ± 0.05	3.783SerGlu: 3.783 ± 0.059	2.893SerPhe: 2.893 ± 0.049	5.596SerGly: 5.596 ± 0.089	1.218SerHis: 1.218 ± 0.036	4.329SerIle: 4.329 ± 0.056	2.938SerLys: 2.938 ± 0.057	6.189SerLeu: 6.189 ± 0.066	1.77SerMet: 1.77 ± 0.033	2.534SerAsn: 2.534 ± 0.052	2.504SerPro: 2.504 ± 0.056	2.095SerGln: 2.095 ± 0.04	3.256SerArg: 3.256 ± 0.054	4.566SerSer: 4.566 ± 0.075	3.448SerThr: 3.448 ± 0.053	4.646SerVal: 4.646 ± 0.071	0.838SerTrp: 0.838 ± 0.022	2.288SerTyr: 2.288 ± 0.047	0.001SerXaa: 0.001 ± 0.001
Thr	5.379ThrAla: 5.379 ± 0.085	0.345ThrCys: 0.345 ± 0.013	2.974ThrAsp: 2.974 ± 0.047	3.19ThrGlu: 3.19 ± 0.051	2.351ThrPhe: 2.351 ± 0.044	4.781ThrGly: 4.781 ± 0.103	0.987ThrHis: 0.987 ± 0.026	3.932ThrIle: 3.932 ± 0.064	2.477ThrLys: 2.477 ± 0.044	5.363ThrLeu: 5.363 ± 0.063	1.372ThrMet: 1.372 ± 0.027	2.205ThrAsn: 2.205 ± 0.054	2.555ThrPro: 2.555 ± 0.053	1.597ThrGln: 1.597 ± 0.03	2.376ThrArg: 2.376 ± 0.038	3.52ThrSer: 3.52 ± 0.061	3.287ThrThr: 3.287 ± 0.075	4.753ThrVal: 4.753 ± 0.089	0.61ThrTrp: 0.61 ± 0.022	1.962ThrTyr: 1.962 ± 0.05	0.0ThrXaa: 0.0 ± 0.0
Val	5.85ValAla: 5.85 ± 0.069	0.638ValCys: 0.638 ± 0.023	3.753ValAsp: 3.753 ± 0.049	4.318ValGlu: 4.318 ± 0.06	2.733ValPhe: 2.733 ± 0.045	4.811ValGly: 4.811 ± 0.064	1.48ValHis: 1.48 ± 0.031	4.657ValIle: 4.657 ± 0.065	3.577ValLys: 3.577 ± 0.053	6.924ValLeu: 6.924 ± 0.081	1.911ValMet: 1.911 ± 0.034	2.864ValAsn: 2.864 ± 0.055	3.116ValPro: 3.116 ± 0.049	2.841ValGln: 2.841 ± 0.043	3.817ValArg: 3.817 ± 0.055	4.952ValSer: 4.952 ± 0.066	4.709ValThr: 4.709 ± 0.095	5.297ValVal: 5.297 ± 0.066	0.858ValTrp: 0.858 ± 0.025	2.426ValTyr: 2.426 ± 0.042	0.0ValXaa: 0.0 ± 0.0
Trp	0.944TrpAla: 0.944 ± 0.027	0.089TrpCys: 0.089 ± 0.008	0.661TrpAsp: 0.661 ± 0.017	0.769TrpGlu: 0.769 ± 0.024	0.533TrpPhe: 0.533 ± 0.019	0.868TrpGly: 0.868 ± 0.026	0.302TrpHis: 0.302 ± 0.013	0.812TrpIle: 0.812 ± 0.025	0.688TrpLys: 0.688 ± 0.02	1.486TrpLeu: 1.486 ± 0.039	0.442TrpMet: 0.442 ± 0.017	0.629TrpAsn: 0.629 ± 0.021	0.411TrpPro: 0.411 ± 0.018	0.533TrpGln: 0.533 ± 0.021	0.709TrpArg: 0.709 ± 0.025	0.948TrpSer: 0.948 ± 0.032	0.805TrpThr: 0.805 ± 0.03	0.758TrpVal: 0.758 ± 0.021	0.191TrpTrp: 0.191 ± 0.011	0.382TrpTyr: 0.382 ± 0.014	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.99TyrAla: 2.99 ± 0.045	0.299TyrCys: 0.299 ± 0.015	1.996TyrAsp: 1.996 ± 0.053	2.235TyrGlu: 2.235 ± 0.037	1.308TyrPhe: 1.308 ± 0.033	2.729TyrGly: 2.729 ± 0.051	0.682TyrHis: 0.682 ± 0.019	1.844TyrIle: 1.844 ± 0.032	1.511TyrLys: 1.511 ± 0.032	3.116TyrLeu: 3.116 ± 0.05	0.89TyrMet: 0.89 ± 0.026	1.313TyrAsn: 1.313 ± 0.036	1.421TyrPro: 1.421 ± 0.032	1.163TyrGln: 1.163 ± 0.029	2.094TyrArg: 2.094 ± 0.034	2.071TyrSer: 2.071 ± 0.042	1.855TyrThr: 1.855 ± 0.049	2.476TyrVal: 2.476 ± 0.04	0.5TyrTrp: 0.5 ± 0.017	1.363TyrTyr: 1.363 ± 0.035	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.001XaaAla: 0.001 ± 0.001	0.0XaaCys: 0.0 ± 0.0	0.001XaaAsp: 0.001 ± 0.001	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.001XaaGly: 0.001 ± 0.001	0.0XaaHis: 0.0 ± 0.0	0.001XaaIle: 0.001 ± 0.001	0.001XaaLys: 0.001 ± 0.001	0.001XaaLeu: 0.001 ± 0.001	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.001XaaGln: 0.001 ± 0.001	0.001XaaArg: 0.001 ± 0.001	0.0XaaSer: 0.0 ± 0.0	0.001XaaThr: 0.001 ± 0.001	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.024XaaXaa: 0.024 ± 0.011

Statistics based on 4803 proteins (1587121 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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