Haloprofundus marisrubri

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Haloferacales; Haloferacaceae; Haloprofundus

Average proteome isoelectric point is 5.02

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3761 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A0W1RD70|A0A0W1RD70_9EURY Peptidase M24 OS=Haloprofundus marisrubri OX=1514971 GN=AUR64_06345 PE=3 SV=1
MM1 pKa = 7.44KK2 pKa = 10.09FDD4 pKa = 3.72PSRR7 pKa = 11.84RR8 pKa = 11.84KK9 pKa = 9.96VLALGAGAALASTGLGSAADD29 pKa = 4.09LEE31 pKa = 4.79GAQNIDD37 pKa = 2.99SHH39 pKa = 6.16PTVDD43 pKa = 4.27AEE45 pKa = 4.61VVDD48 pKa = 4.21SFTDD52 pKa = 3.89GLEE55 pKa = 4.15LNVTISVTDD64 pKa = 3.87PDD66 pKa = 5.07DD67 pKa = 3.73EE68 pKa = 4.38VDD70 pKa = 3.84RR71 pKa = 11.84IGFIVEE77 pKa = 4.1NYY79 pKa = 10.07NDD81 pKa = 3.85RR82 pKa = 11.84TVDD85 pKa = 4.04IATHH89 pKa = 6.21WFNEE93 pKa = 3.74ASGEE97 pKa = 4.2KK98 pKa = 8.99TVSKK102 pKa = 10.66VVSLNRR108 pKa = 11.84APYY111 pKa = 9.58ACYY114 pKa = 10.5AFVKK118 pKa = 10.4AGSDD122 pKa = 4.03YY123 pKa = 11.26YY124 pKa = 11.47SVDD127 pKa = 3.33SVRR130 pKa = 11.84LGPEE134 pKa = 3.74TEE136 pKa = 4.36APAPQLLGVDD146 pKa = 3.91VSGTTATIDD155 pKa = 3.72YY156 pKa = 8.08EE157 pKa = 4.43VSPDD161 pKa = 3.3EE162 pKa = 4.07PAEE165 pKa = 4.05YY166 pKa = 10.47SVGASVSPISGSSSGDD182 pKa = 3.12PVYY185 pKa = 8.44TTEE188 pKa = 5.45RR189 pKa = 11.84NDD191 pKa = 3.87PYY193 pKa = 11.32DD194 pKa = 3.81EE195 pKa = 4.4PTTARR200 pKa = 11.84TVVTGLAPNTEE211 pKa = 4.36YY212 pKa = 10.23TATAWTEE219 pKa = 3.6LDD221 pKa = 2.9TWAMKK226 pKa = 9.8EE227 pKa = 3.76QSDD230 pKa = 4.25YY231 pKa = 11.88AEE233 pKa = 5.15SEE235 pKa = 4.22TQTFKK240 pKa = 10.76TGDD243 pKa = 3.43VVAPEE248 pKa = 4.59PEE250 pKa = 4.24TEE252 pKa = 3.8HH253 pKa = 7.3RR254 pKa = 11.84LVIGGSSDD262 pKa = 3.01VSYY265 pKa = 11.43YY266 pKa = 10.71RR267 pKa = 11.84VTTTGSLEE275 pKa = 4.11QTTEE279 pKa = 3.77TGDD282 pKa = 3.65APFSSATVDD291 pKa = 3.64DD292 pKa = 4.27EE293 pKa = 5.86DD294 pKa = 4.39YY295 pKa = 11.59VSGSSACGGVAGGADD310 pKa = 3.01AYY312 pKa = 11.4VFTGEE317 pKa = 4.13LTNVGVDD324 pKa = 4.02GAASVYY330 pKa = 10.81LDD332 pKa = 4.0GEE334 pKa = 4.56EE335 pKa = 5.03IDD337 pKa = 3.86PADD340 pKa = 3.82YY341 pKa = 11.19ADD343 pKa = 4.65EE344 pKa = 4.88LPHH347 pKa = 7.2HH348 pKa = 6.53LAITGGSDD356 pKa = 2.8LTEE359 pKa = 3.72YY360 pKa = 11.04AFRR363 pKa = 11.84VSGDD367 pKa = 3.58VIKK370 pKa = 8.46TTEE373 pKa = 3.66VDD375 pKa = 3.47GTGLSLNPSYY385 pKa = 11.2DD386 pKa = 3.91DD387 pKa = 4.14EE388 pKa = 4.76DD389 pKa = 4.24TVSAGQGAGATAGGTDD405 pKa = 3.86AYY407 pKa = 10.37RR408 pKa = 11.84FSSPQVTSAEE418 pKa = 3.99EE419 pKa = 4.82DD420 pKa = 3.1ISFTQFDD427 pKa = 3.88GDD429 pKa = 3.56ATVYY433 pKa = 11.11LNGEE437 pKa = 4.4EE438 pKa = 4.83IDD440 pKa = 3.9PADD443 pKa = 3.6YY444 pKa = 11.29

Molecular weight:
46.63 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A0W1REM5|A0A0W1REM5_9EURY Ferredoxin OS=Haloprofundus marisrubri OX=1514971 GN=AUR64_03090 PE=4 SV=1
MM1 pKa = 7.62SSGRR5 pKa = 11.84RR6 pKa = 11.84LRR8 pKa = 11.84IRR10 pKa = 11.84PEE12 pKa = 3.57PRR14 pKa = 11.84LEE16 pKa = 4.16TLHH19 pKa = 6.62EE20 pKa = 4.14RR21 pKa = 11.84AFKK24 pKa = 10.73YY25 pKa = 8.9ATLSIIGQNAPFLLAIEE42 pKa = 4.54PVRR45 pKa = 11.84EE46 pKa = 3.95SSPWGDD52 pKa = 3.28NPSNRR57 pKa = 11.84IHH59 pKa = 6.27RR60 pKa = 11.84VVRR63 pKa = 11.84RR64 pKa = 11.84LVRR67 pKa = 11.84RR68 pKa = 11.84AKK70 pKa = 9.82EE71 pKa = 3.68LVPIEE76 pKa = 4.14MVLCDD81 pKa = 4.62RR82 pKa = 11.84EE83 pKa = 4.31FDD85 pKa = 4.5SMDD88 pKa = 3.35AFQTLSNPNVNYY100 pKa = 10.26LIPKK104 pKa = 9.4RR105 pKa = 11.84VTSTEE110 pKa = 4.05STRR113 pKa = 11.84KK114 pKa = 9.66

Molecular weight:
13.24 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3761

0

3761

1074478

43

1681

285.7

31.11

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.431 ± 0.051

0.687 ± 0.013

7.996 ± 0.046

8.3 ± 0.051

3.554 ± 0.027

8.427 ± 0.045

1.978 ± 0.02

3.738 ± 0.033

2.0 ± 0.024

9.161 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.774 ± 0.018

2.556 ± 0.024

4.493 ± 0.024

2.559 ± 0.024

6.557 ± 0.04

6.068 ± 0.038

6.427 ± 0.037

9.361 ± 0.042

1.147 ± 0.015

2.786 ± 0.02

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski