Haloprofundus marisrubri
Average proteome isoelectric point is 5.02
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3761 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0W1RD70|A0A0W1RD70_9EURY Peptidase M24 OS=Haloprofundus marisrubri OX=1514971 GN=AUR64_06345 PE=3 SV=1
MM1 pKa = 7.44 KK2 pKa = 10.09 FDD4 pKa = 3.72 PSRR7 pKa = 11.84 RR8 pKa = 11.84 KK9 pKa = 9.96 VLALGAGAALASTGLGSAADD29 pKa = 4.09 LEE31 pKa = 4.79 GAQNIDD37 pKa = 2.99 SHH39 pKa = 6.16 PTVDD43 pKa = 4.27 AEE45 pKa = 4.61 VVDD48 pKa = 4.21 SFTDD52 pKa = 3.89 GLEE55 pKa = 4.15 LNVTISVTDD64 pKa = 3.87 PDD66 pKa = 5.07 DD67 pKa = 3.73 EE68 pKa = 4.38 VDD70 pKa = 3.84 RR71 pKa = 11.84 IGFIVEE77 pKa = 4.1 NYY79 pKa = 10.07 NDD81 pKa = 3.85 RR82 pKa = 11.84 TVDD85 pKa = 4.04 IATHH89 pKa = 6.21 WFNEE93 pKa = 3.74 ASGEE97 pKa = 4.2 KK98 pKa = 8.99 TVSKK102 pKa = 10.66 VVSLNRR108 pKa = 11.84 APYY111 pKa = 9.58 ACYY114 pKa = 10.5 AFVKK118 pKa = 10.4 AGSDD122 pKa = 4.03 YY123 pKa = 11.26 YY124 pKa = 11.47 SVDD127 pKa = 3.33 SVRR130 pKa = 11.84 LGPEE134 pKa = 3.74 TEE136 pKa = 4.36 APAPQLLGVDD146 pKa = 3.91 VSGTTATIDD155 pKa = 3.72 YY156 pKa = 8.08 EE157 pKa = 4.43 VSPDD161 pKa = 3.3 EE162 pKa = 4.07 PAEE165 pKa = 4.05 YY166 pKa = 10.47 SVGASVSPISGSSSGDD182 pKa = 3.12 PVYY185 pKa = 8.44 TTEE188 pKa = 5.45 RR189 pKa = 11.84 NDD191 pKa = 3.87 PYY193 pKa = 11.32 DD194 pKa = 3.81 EE195 pKa = 4.4 PTTARR200 pKa = 11.84 TVVTGLAPNTEE211 pKa = 4.36 YY212 pKa = 10.23 TATAWTEE219 pKa = 3.6 LDD221 pKa = 2.9 TWAMKK226 pKa = 9.8 EE227 pKa = 3.76 QSDD230 pKa = 4.25 YY231 pKa = 11.88 AEE233 pKa = 5.15 SEE235 pKa = 4.22 TQTFKK240 pKa = 10.76 TGDD243 pKa = 3.43 VVAPEE248 pKa = 4.59 PEE250 pKa = 4.24 TEE252 pKa = 3.8 HH253 pKa = 7.3 RR254 pKa = 11.84 LVIGGSSDD262 pKa = 3.01 VSYY265 pKa = 11.43 YY266 pKa = 10.71 RR267 pKa = 11.84 VTTTGSLEE275 pKa = 4.11 QTTEE279 pKa = 3.77 TGDD282 pKa = 3.65 APFSSATVDD291 pKa = 3.64 DD292 pKa = 4.27 EE293 pKa = 5.86 DD294 pKa = 4.39 YY295 pKa = 11.59 VSGSSACGGVAGGADD310 pKa = 3.01 AYY312 pKa = 11.4 VFTGEE317 pKa = 4.13 LTNVGVDD324 pKa = 4.02 GAASVYY330 pKa = 10.81 LDD332 pKa = 4.0 GEE334 pKa = 4.56 EE335 pKa = 5.03 IDD337 pKa = 3.86 PADD340 pKa = 3.82 YY341 pKa = 11.19 ADD343 pKa = 4.65 EE344 pKa = 4.88 LPHH347 pKa = 7.2 HH348 pKa = 6.53 LAITGGSDD356 pKa = 2.8 LTEE359 pKa = 3.72 YY360 pKa = 11.04 AFRR363 pKa = 11.84 VSGDD367 pKa = 3.58 VIKK370 pKa = 8.46 TTEE373 pKa = 3.66 VDD375 pKa = 3.47 GTGLSLNPSYY385 pKa = 11.2 DD386 pKa = 3.91 DD387 pKa = 4.14 EE388 pKa = 4.76 DD389 pKa = 4.24 TVSAGQGAGATAGGTDD405 pKa = 3.86 AYY407 pKa = 10.37 RR408 pKa = 11.84 FSSPQVTSAEE418 pKa = 3.99 EE419 pKa = 4.82 DD420 pKa = 3.1 ISFTQFDD427 pKa = 3.88 GDD429 pKa = 3.56 ATVYY433 pKa = 11.11 LNGEE437 pKa = 4.4 EE438 pKa = 4.83 IDD440 pKa = 3.9 PADD443 pKa = 3.6 YY444 pKa = 11.29
Molecular weight: 46.63 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.705
IPC2_protein 3.694
IPC_protein 3.719
Toseland 3.503
ProMoST 3.872
Dawson 3.706
Bjellqvist 3.859
Wikipedia 3.63
Rodwell 3.541
Grimsley 3.401
Solomon 3.694
Lehninger 3.656
Nozaki 3.808
DTASelect 4.05
Thurlkill 3.541
EMBOSS 3.643
Sillero 3.834
Patrickios 0.935
IPC_peptide 3.694
IPC2_peptide 3.821
IPC2.peptide.svr19 3.747
Protein with the highest isoelectric point:
>tr|A0A0W1REM5|A0A0W1REM5_9EURY Ferredoxin OS=Haloprofundus marisrubri OX=1514971 GN=AUR64_03090 PE=4 SV=1
MM1 pKa = 7.62 SSGRR5 pKa = 11.84 RR6 pKa = 11.84 LRR8 pKa = 11.84 IRR10 pKa = 11.84 PEE12 pKa = 3.57 PRR14 pKa = 11.84 LEE16 pKa = 4.16 TLHH19 pKa = 6.62 EE20 pKa = 4.14 RR21 pKa = 11.84 AFKK24 pKa = 10.73 YY25 pKa = 8.9 ATLSIIGQNAPFLLAIEE42 pKa = 4.54 PVRR45 pKa = 11.84 EE46 pKa = 3.95 SSPWGDD52 pKa = 3.28 NPSNRR57 pKa = 11.84 IHH59 pKa = 6.27 RR60 pKa = 11.84 VVRR63 pKa = 11.84 RR64 pKa = 11.84 LVRR67 pKa = 11.84 RR68 pKa = 11.84 AKK70 pKa = 9.82 EE71 pKa = 3.68 LVPIEE76 pKa = 4.14 MVLCDD81 pKa = 4.62 RR82 pKa = 11.84 EE83 pKa = 4.31 FDD85 pKa = 4.5 SMDD88 pKa = 3.35 AFQTLSNPNVNYY100 pKa = 10.26 LIPKK104 pKa = 9.4 RR105 pKa = 11.84 VTSTEE110 pKa = 4.05 STRR113 pKa = 11.84 KK114 pKa = 9.66
Molecular weight: 13.24 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.347
IPC2_protein 9.487
IPC_protein 10.452
Toseland 10.657
ProMoST 10.54
Dawson 10.745
Bjellqvist 10.496
Wikipedia 10.994
Rodwell 10.804
Grimsley 10.789
Solomon 10.921
Lehninger 10.877
Nozaki 10.643
DTASelect 10.496
Thurlkill 10.657
EMBOSS 11.082
Sillero 10.687
Patrickios 10.57
IPC_peptide 10.921
IPC2_peptide 9.56
IPC2.peptide.svr19 8.744
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3761
0
3761
1074478
43
1681
285.7
31.11
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.431 ± 0.051
0.687 ± 0.013
7.996 ± 0.046
8.3 ± 0.051
3.554 ± 0.027
8.427 ± 0.045
1.978 ± 0.02
3.738 ± 0.033
2.0 ± 0.024
9.161 ± 0.055
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.774 ± 0.018
2.556 ± 0.024
4.493 ± 0.024
2.559 ± 0.024
6.557 ± 0.04
6.068 ± 0.038
6.427 ± 0.037
9.361 ± 0.042
1.147 ± 0.015
2.786 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here