Halomonas chromatireducens

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Oceanospirillales; Halomonadaceae; Halomonas

Average proteome isoelectric point is 6.1

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3678 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A125R079|A0A125R079_9GAMM Sialic acid TRAP transporter permease protein SiaT OS=Halomonas chromatireducens OX=507626 GN=siaT_15 PE=4 SV=1
MM1 pKa = 7.57SGFNIISEE9 pKa = 4.2GDD11 pKa = 3.41HH12 pKa = 6.44FDD14 pKa = 3.12SDD16 pKa = 3.82GEE18 pKa = 4.1YY19 pKa = 10.77RR20 pKa = 11.84PFGYY24 pKa = 10.52LDD26 pKa = 3.82GEE28 pKa = 4.3EE29 pKa = 4.56MPDD32 pKa = 3.24GTTCSTDD39 pKa = 2.78IDD41 pKa = 4.27KK42 pKa = 10.91QCQPSRR48 pKa = 11.84GKK50 pKa = 10.41FSAGGSSAGDD60 pKa = 3.29GVLIIDD66 pKa = 4.65GNVEE70 pKa = 4.19FNGNPEE76 pKa = 4.01FQGLIIVLGDD86 pKa = 3.45YY87 pKa = 7.45TVKK90 pKa = 10.92GGGGGDD96 pKa = 3.66LEE98 pKa = 4.53GSIIAAPYY106 pKa = 9.06SCRR109 pKa = 11.84DD110 pKa = 3.45GNCVFNEE117 pKa = 3.88VNIDD121 pKa = 3.55LTGGGGNDD129 pKa = 3.43YY130 pKa = 11.21LHH132 pKa = 6.84VLEE135 pKa = 5.02YY136 pKa = 11.09LDD138 pKa = 5.29AAWEE142 pKa = 4.1LLGSVSPEE150 pKa = 3.62AARR153 pKa = 11.84LWLEE157 pKa = 3.95GNNPGGNFTYY167 pKa = 9.89MARR170 pKa = 11.84GWRR173 pKa = 11.84EE174 pKa = 3.25ITVPQQ179 pKa = 3.81

Molecular weight: 19.02 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0X8HD28|A0A0X8HD28_9GAMM Lipoprotein-releasing system transmembrane protein LolE OS=Halomonas chromatireducens OX=507626 GN=lolE PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.34RR12 pKa = 11.84KK13 pKa = 9.1RR14 pKa = 11.84VHH16 pKa = 6.26GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.16NGRR28 pKa = 11.84AVLSRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.99GRR39 pKa = 11.84KK40 pKa = 8.85RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight: 5.17 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3678

0

3678

1174037

29

2365

319.2

35.06

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.047 ± 0.049

0.928 ± 0.013

5.698 ± 0.042

6.759 ± 0.048

3.462 ± 0.026

8.259 ± 0.033

2.491 ± 0.023

4.726 ± 0.034

2.575 ± 0.033

11.591 ± 0.065

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.617 ± 0.023

2.443 ± 0.024

4.989 ± 0.028

3.776 ± 0.026

7.413 ± 0.049

5.433 ± 0.027

4.874 ± 0.021

7.156 ± 0.035

1.463 ± 0.021

2.293 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski