Ferrovum sp. PN-J185

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Ferrovales; Ferrovaceae; Ferrovum; unclassified Ferrovum

Average proteome isoelectric point is 6.69

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1842 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A149VTK5|A0A149VTK5_9PROT Lipopolysaccharide export system ATP-binding protein LptB OS=Ferrovum sp. PN-J185 OX=1356306 GN=lptB_1 PE=4 SV=1
MM1 pKa = 6.48HH2 pKa = 7.78TIYY5 pKa = 10.74KK6 pKa = 9.09FEE8 pKa = 4.34CFKK11 pKa = 11.19SFNVTDD17 pKa = 4.07IYY19 pKa = 11.55KK20 pKa = 10.16NDD22 pKa = 3.47QDD24 pKa = 4.35DD25 pKa = 4.68EE26 pKa = 4.48IVITNLWTYY35 pKa = 9.39ATLEE39 pKa = 4.15YY40 pKa = 11.27NEE42 pKa = 5.42DD43 pKa = 3.72EE44 pKa = 4.8LPEE47 pKa = 5.78KK48 pKa = 10.27EE49 pKa = 5.34DD50 pKa = 3.06NGNYY54 pKa = 10.01YY55 pKa = 10.47DD56 pKa = 3.92IFLPEE61 pKa = 5.09AFDD64 pKa = 4.4CSDD67 pKa = 3.76LFVSNVDD74 pKa = 4.29YY75 pKa = 10.59FIEE78 pKa = 5.14DD79 pKa = 3.18NKK81 pKa = 11.03LLNKK85 pKa = 10.11ISALEE90 pKa = 4.29DD91 pKa = 3.06QDD93 pKa = 5.12KK94 pKa = 11.07FIDD97 pKa = 5.0LIQANGFEE105 pKa = 4.52HH106 pKa = 7.46FEE108 pKa = 4.1TQYY111 pKa = 11.61DD112 pKa = 3.59ILDD115 pKa = 3.95SGEE118 pKa = 3.81GSIVVVEE125 pKa = 4.27VV126 pKa = 3.12

Molecular weight: 14.8 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A149VRT7|A0A149VRT7_9PROT 1-acyl-sn-glycerol-3-phosphate acyltransferase OS=Ferrovum sp. PN-J185 OX=1356306 GN=plsC_1 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.36RR3 pKa = 11.84TYY5 pKa = 10.14QPSVTKK11 pKa = 10.56RR12 pKa = 11.84KK13 pKa = 7.62RR14 pKa = 11.84THH16 pKa = 5.71GFRR19 pKa = 11.84VRR21 pKa = 11.84MKK23 pKa = 9.39TRR25 pKa = 11.84GGRR28 pKa = 11.84AVINARR34 pKa = 11.84RR35 pKa = 11.84SRR37 pKa = 11.84GRR39 pKa = 11.84SRR41 pKa = 11.84LAVV44 pKa = 3.35

Molecular weight: 5.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1842

0

1842

579268

31

1541

314.5

35.08

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.871 ± 0.066

0.918 ± 0.018

5.013 ± 0.041

5.672 ± 0.052

4.146 ± 0.036

6.813 ± 0.061

2.704 ± 0.032

7.164 ± 0.051

5.249 ± 0.048

10.817 ± 0.066

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.42 ± 0.029

4.409 ± 0.045

4.254 ± 0.032

4.574 ± 0.044

4.889 ± 0.053

6.381 ± 0.045

5.504 ± 0.038

6.928 ± 0.047

1.284 ± 0.024

2.989 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski