Amino acid dipepetide frequency for Ferrovum sp. PN-J185

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.475AlaAla: 6.475 ± 0.161
0.779AlaCys: 0.779 ± 0.036
3.598AlaAsp: 3.598 ± 0.092
4.164AlaGlu: 4.164 ± 0.095
2.964AlaPhe: 2.964 ± 0.074
5.56AlaGly: 5.56 ± 0.118
1.984AlaHis: 1.984 ± 0.065
6.035AlaIle: 6.035 ± 0.115
4.575AlaLys: 4.575 ± 0.108
9.469AlaLeu: 9.469 ± 0.141
2.213AlaMet: 2.213 ± 0.07
3.249AlaAsn: 3.249 ± 0.082
2.852AlaPro: 2.852 ± 0.077
3.945AlaGln: 3.945 ± 0.092
3.8AlaArg: 3.8 ± 0.085
4.345AlaSer: 4.345 ± 0.08
4.212AlaThr: 4.212 ± 0.089
5.374AlaVal: 5.374 ± 0.109
0.898AlaTrp: 0.898 ± 0.037
2.223AlaTyr: 2.223 ± 0.07
0.0AlaXaa: 0.0 ± 0.0
Cys
0.71CysAla: 0.71 ± 0.035
0.124CysCys: 0.124 ± 0.015
0.528CysAsp: 0.528 ± 0.033
0.445CysGlu: 0.445 ± 0.027
0.399CysPhe: 0.399 ± 0.031
0.886CysGly: 0.886 ± 0.038
0.402CysHis: 0.402 ± 0.027
0.527CysIle: 0.527 ± 0.033
0.281CysLys: 0.281 ± 0.023
0.899CysLeu: 0.899 ± 0.033
0.171CysMet: 0.171 ± 0.019
0.333CysAsn: 0.333 ± 0.027
0.558CysPro: 0.558 ± 0.032
0.421CysGln: 0.421 ± 0.029
0.497CysArg: 0.497 ± 0.028
0.539CysSer: 0.539 ± 0.032
0.423CysThr: 0.423 ± 0.027
0.656CysVal: 0.656 ± 0.041
0.097CysTrp: 0.097 ± 0.014
0.288CysTyr: 0.288 ± 0.023
0.0CysXaa: 0.0 ± 0.0
Asp
3.653AspAla: 3.653 ± 0.087
0.502AspCys: 0.502 ± 0.028
2.137AspAsp: 2.137 ± 0.065
3.347AspGlu: 3.347 ± 0.089
2.279AspPhe: 2.279 ± 0.064
3.021AspGly: 3.021 ± 0.076
1.272AspHis: 1.272 ± 0.043
3.717AspIle: 3.717 ± 0.094
2.672AspLys: 2.672 ± 0.059
5.327AspLeu: 5.327 ± 0.113
1.281AspMet: 1.281 ± 0.046
2.237AspAsn: 2.237 ± 0.063
2.291AspPro: 2.291 ± 0.072
2.263AspGln: 2.263 ± 0.066
2.593AspArg: 2.593 ± 0.065
2.845AspSer: 2.845 ± 0.071
2.56AspThr: 2.56 ± 0.067
3.394AspVal: 3.394 ± 0.091
0.804AspTrp: 0.804 ± 0.041
1.933AspTyr: 1.933 ± 0.063
0.0AspXaa: 0.0 ± 0.0
Glu
4.877GluAla: 4.877 ± 0.118
0.459GluCys: 0.459 ± 0.026
2.491GluAsp: 2.491 ± 0.064
3.594GluGlu: 3.594 ± 0.098
2.165GluPhe: 2.165 ± 0.065
3.437GluGly: 3.437 ± 0.074
1.5GluHis: 1.5 ± 0.053
4.06GluIle: 4.06 ± 0.094
3.677GluLys: 3.677 ± 0.085
6.273GluLeu: 6.273 ± 0.118
1.455GluMet: 1.455 ± 0.052
2.28GluAsn: 2.28 ± 0.061
1.821GluPro: 1.821 ± 0.058
2.893GluGln: 2.893 ± 0.082
3.358GluArg: 3.358 ± 0.093
3.335GluSer: 3.335 ± 0.084
2.874GluThr: 2.874 ± 0.074
4.074GluVal: 4.074 ± 0.098
0.756GluTrp: 0.756 ± 0.037
1.334GluTyr: 1.334 ± 0.051
0.0GluXaa: 0.0 ± 0.0
Phe
3.154PheAla: 3.154 ± 0.077
0.423PheCys: 0.423 ± 0.032
2.526PheAsp: 2.526 ± 0.062
2.186PheGlu: 2.186 ± 0.07
1.901PhePhe: 1.901 ± 0.062
3.066PheGly: 3.066 ± 0.078
0.937PheHis: 0.937 ± 0.046
3.208PheIle: 3.208 ± 0.081
2.156PheLys: 2.156 ± 0.058
3.788PheLeu: 3.788 ± 0.087
0.96PheMet: 0.96 ± 0.045
2.351PheAsn: 2.351 ± 0.073
1.711PhePro: 1.711 ± 0.061
1.309PheGln: 1.309 ± 0.045
1.573PheArg: 1.573 ± 0.047
3.189PheSer: 3.189 ± 0.084
2.367PheThr: 2.367 ± 0.069
2.764PheVal: 2.764 ± 0.069
0.527PheTrp: 0.527 ± 0.033
1.369PheTyr: 1.369 ± 0.054
0.0PheXaa: 0.0 ± 0.0
Gly
5.186GlyAla: 5.186 ± 0.107
0.711GlyCys: 0.711 ± 0.04
3.18GlyAsp: 3.18 ± 0.07
3.675GlyGlu: 3.675 ± 0.082
3.366GlyPhe: 3.366 ± 0.08
4.827GlyGly: 4.827 ± 0.103
1.839GlyHis: 1.839 ± 0.057
5.384GlyIle: 5.384 ± 0.098
3.622GlyLys: 3.622 ± 0.078
7.314GlyLeu: 7.314 ± 0.137
1.901GlyMet: 1.901 ± 0.061
2.353GlyAsn: 2.353 ± 0.078
2.066GlyPro: 2.066 ± 0.067
2.959GlyGln: 2.959 ± 0.083
3.409GlyArg: 3.409 ± 0.08
4.264GlySer: 4.264 ± 0.098
3.325GlyThr: 3.325 ± 0.076
5.415GlyVal: 5.415 ± 0.115
0.965GlyTrp: 0.965 ± 0.045
2.369GlyTyr: 2.369 ± 0.063
0.0GlyXaa: 0.0 ± 0.0
His
1.942HisAla: 1.942 ± 0.062
0.319HisCys: 0.319 ± 0.026
1.307HisAsp: 1.307 ± 0.045
1.435HisGlu: 1.435 ± 0.057
1.338HisPhe: 1.338 ± 0.058
1.814HisGly: 1.814 ± 0.069
1.129HisHis: 1.129 ± 0.057
1.887HisIle: 1.887 ± 0.061
0.934HisLys: 0.934 ± 0.045
2.816HisLeu: 2.816 ± 0.073
0.523HisMet: 0.523 ± 0.035
1.055HisAsn: 1.055 ± 0.044
1.473HisPro: 1.473 ± 0.05
1.457HisGln: 1.457 ± 0.058
1.364HisArg: 1.364 ± 0.05
1.664HisSer: 1.664 ± 0.055
1.426HisThr: 1.426 ± 0.058
1.645HisVal: 1.645 ± 0.058
0.457HisTrp: 0.457 ± 0.029
1.053HisTyr: 1.053 ± 0.043
0.0HisXaa: 0.0 ± 0.0
Ile
5.937IleAla: 5.937 ± 0.123
0.64IleCys: 0.64 ± 0.03
4.416IleAsp: 4.416 ± 0.086
4.723IleGlu: 4.723 ± 0.097
2.488IlePhe: 2.488 ± 0.08
4.898IleGly: 4.898 ± 0.082
1.984IleHis: 1.984 ± 0.06
4.697IleIle: 4.697 ± 0.121
4.262IleLys: 4.262 ± 0.082
6.496IleLeu: 6.496 ± 0.116
1.474IleMet: 1.474 ± 0.056
3.983IleAsn: 3.983 ± 0.092
3.427IlePro: 3.427 ± 0.081
2.755IleGln: 2.755 ± 0.069
3.218IleArg: 3.218 ± 0.071
4.737IleSer: 4.737 ± 0.117
4.226IleThr: 4.226 ± 0.09
4.597IleVal: 4.597 ± 0.085
0.68IleTrp: 0.68 ± 0.04
1.999IleTyr: 1.999 ± 0.07
0.0IleXaa: 0.0 ± 0.0
Lys
4.171LysAla: 4.171 ± 0.102
0.28LysCys: 0.28 ± 0.029
2.66LysAsp: 2.66 ± 0.073
3.82LysGlu: 3.82 ± 0.082
1.371LysPhe: 1.371 ± 0.048
3.154LysGly: 3.154 ± 0.076
1.326LysHis: 1.326 ± 0.049
3.63LysIle: 3.63 ± 0.089
3.573LysLys: 3.573 ± 0.103
4.998LysLeu: 4.998 ± 0.1
1.269LysMet: 1.269 ± 0.052
2.703LysAsn: 2.703 ± 0.074
2.331LysPro: 2.331 ± 0.072
2.833LysGln: 2.833 ± 0.079
2.831LysArg: 2.831 ± 0.066
3.137LysSer: 3.137 ± 0.087
3.183LysThr: 3.183 ± 0.074
3.356LysVal: 3.356 ± 0.086
0.552LysTrp: 0.552 ± 0.028
1.315LysTyr: 1.315 ± 0.058
0.0LysXaa: 0.0 ± 0.0
Leu
8.954LeuAla: 8.954 ± 0.147
1.032LeuCys: 1.032 ± 0.047
5.3LeuAsp: 5.3 ± 0.102
5.609LeuGlu: 5.609 ± 0.12
4.721LeuPhe: 4.721 ± 0.121
7.197LeuGly: 7.197 ± 0.136
2.496LeuHis: 2.496 ± 0.069
8.103LeuIle: 8.103 ± 0.132
5.945LeuLys: 5.945 ± 0.118
11.326LeuLeu: 11.326 ± 0.175
2.697LeuMet: 2.697 ± 0.076
5.108LeuAsn: 5.108 ± 0.101
4.953LeuPro: 4.953 ± 0.09
3.658LeuGln: 3.658 ± 0.082
4.965LeuArg: 4.965 ± 0.104
8.552LeuSer: 8.552 ± 0.13
6.722LeuThr: 6.722 ± 0.133
6.921LeuVal: 6.921 ± 0.108
1.215LeuTrp: 1.215 ± 0.057
2.697LeuTyr: 2.697 ± 0.078
0.0LeuXaa: 0.0 ± 0.0
Met
2.389MetAla: 2.389 ± 0.071
0.164MetCys: 0.164 ± 0.016
1.219MetAsp: 1.219 ± 0.047
1.119MetGlu: 1.119 ± 0.049
0.71MetPhe: 0.71 ± 0.037
1.908MetGly: 1.908 ± 0.065
0.508MetHis: 0.508 ± 0.031
1.657MetIle: 1.657 ± 0.05
1.488MetLys: 1.488 ± 0.056
2.237MetLeu: 2.237 ± 0.067
0.704MetMet: 0.704 ± 0.04
1.129MetAsn: 1.129 ± 0.047
1.075MetPro: 1.075 ± 0.045
0.879MetGln: 0.879 ± 0.038
1.229MetArg: 1.229 ± 0.044
1.764MetSer: 1.764 ± 0.059
1.609MetThr: 1.609 ± 0.048
1.744MetVal: 1.744 ± 0.061
0.211MetTrp: 0.211 ± 0.018
0.454MetTyr: 0.454 ± 0.028
0.0MetXaa: 0.0 ± 0.0
Asn
3.223AsnAla: 3.223 ± 0.083
0.432AsnCys: 0.432 ± 0.032
2.198AsnAsp: 2.198 ± 0.067
2.536AsnGlu: 2.536 ± 0.072
1.55AsnPhe: 1.55 ± 0.057
2.608AsnGly: 2.608 ± 0.078
1.403AsnHis: 1.403 ± 0.06
2.973AsnIle: 2.973 ± 0.085
2.377AsnLys: 2.377 ± 0.068
4.252AsnLeu: 4.252 ± 0.091
0.982AsnMet: 0.982 ± 0.039
2.493AsnAsn: 2.493 ± 0.088
2.413AsnPro: 2.413 ± 0.07
2.629AsnGln: 2.629 ± 0.079
2.123AsnArg: 2.123 ± 0.068
2.581AsnSer: 2.581 ± 0.07
2.7AsnThr: 2.7 ± 0.071
2.448AsnVal: 2.448 ± 0.072
0.753AsnTrp: 0.753 ± 0.037
1.414AsnTyr: 1.414 ± 0.052
0.0AsnXaa: 0.0 ± 0.0
Pro
2.681ProAla: 2.681 ± 0.081
0.309ProCys: 0.309 ± 0.024
2.42ProAsp: 2.42 ± 0.066
2.695ProGlu: 2.695 ± 0.07
1.828ProPhe: 1.828 ± 0.05
2.964ProGly: 2.964 ± 0.069
1.262ProHis: 1.262 ± 0.047
3.047ProIle: 3.047 ± 0.076
2.135ProLys: 2.135 ± 0.059
4.611ProLeu: 4.611 ± 0.094
1.013ProMet: 1.013 ± 0.045
1.913ProAsn: 1.913 ± 0.066
1.611ProPro: 1.611 ± 0.054
2.168ProGln: 2.168 ± 0.064
1.633ProArg: 1.633 ± 0.061
2.517ProSer: 2.517 ± 0.069
2.429ProThr: 2.429 ± 0.076
3.32ProVal: 3.32 ± 0.077
0.596ProTrp: 0.596 ± 0.035
1.388ProTyr: 1.388 ± 0.05
0.0ProXaa: 0.0 ± 0.0
Gln
3.777GlnAla: 3.777 ± 0.092
0.397GlnCys: 0.397 ± 0.025
2.054GlnAsp: 2.054 ± 0.06
2.465GlnGlu: 2.465 ± 0.076
1.925GlnPhe: 1.925 ± 0.054
3.116GlnGly: 3.116 ± 0.069
1.474GlnHis: 1.474 ± 0.055
2.881GlnIle: 2.881 ± 0.069
2.054GlnLys: 2.054 ± 0.056
5.718GlnLeu: 5.718 ± 0.124
0.975GlnMet: 0.975 ± 0.042
1.581GlnAsn: 1.581 ± 0.062
1.776GlnPro: 1.776 ± 0.057
2.707GlnGln: 2.707 ± 0.09
2.51GlnArg: 2.51 ± 0.072
2.883GlnSer: 2.883 ± 0.057
2.049GlnThr: 2.049 ± 0.062
3.18GlnVal: 3.18 ± 0.076
0.768GlnTrp: 0.768 ± 0.031
1.447GlnTyr: 1.447 ± 0.053
0.0GlnXaa: 0.0 ± 0.0
Arg
3.724ArgAla: 3.724 ± 0.098
0.416ArgCys: 0.416 ± 0.027
2.57ArgAsp: 2.57 ± 0.08
3.164ArgGlu: 3.164 ± 0.087
2.505ArgPhe: 2.505 ± 0.071
2.969ArgGly: 2.969 ± 0.071
1.376ArgHis: 1.376 ± 0.054
3.435ArgIle: 3.435 ± 0.078
2.051ArgLys: 2.051 ± 0.063
5.719ArgLeu: 5.719 ± 0.107
1.17ArgMet: 1.17 ± 0.046
1.737ArgAsn: 1.737 ± 0.054
1.923ArgPro: 1.923 ± 0.052
2.482ArgGln: 2.482 ± 0.07
2.726ArgArg: 2.726 ± 0.081
2.836ArgSer: 2.836 ± 0.065
2.041ArgThr: 2.041 ± 0.059
3.542ArgVal: 3.542 ± 0.086
0.635ArgTrp: 0.635 ± 0.034
1.871ArgTyr: 1.871 ± 0.058
0.0ArgXaa: 0.0 ± 0.0
Ser
4.708SerAla: 4.708 ± 0.108
0.551SerCys: 0.551 ± 0.033
3.128SerAsp: 3.128 ± 0.073
3.318SerGlu: 3.318 ± 0.071
2.81SerPhe: 2.81 ± 0.087
4.913SerGly: 4.913 ± 0.092
1.858SerHis: 1.858 ± 0.056
4.551SerIle: 4.551 ± 0.104
2.733SerLys: 2.733 ± 0.078
7.627SerLeu: 7.627 ± 0.124
1.609SerMet: 1.609 ± 0.051
2.538SerAsn: 2.538 ± 0.084
2.56SerPro: 2.56 ± 0.064
3.025SerGln: 3.025 ± 0.073
2.968SerArg: 2.968 ± 0.068
4.171SerSer: 4.171 ± 0.093
3.503SerThr: 3.503 ± 0.081
4.495SerVal: 4.495 ± 0.084
0.867SerTrp: 0.867 ± 0.042
1.88SerTyr: 1.88 ± 0.064
0.0SerXaa: 0.0 ± 0.0
Thr
4.062ThrAla: 4.062 ± 0.087
0.475ThrCys: 0.475 ± 0.031
2.602ThrAsp: 2.602 ± 0.055
2.843ThrGlu: 2.843 ± 0.075
2.125ThrPhe: 2.125 ± 0.073
4.015ThrGly: 4.015 ± 0.087
1.649ThrHis: 1.649 ± 0.057
3.907ThrIle: 3.907 ± 0.088
2.436ThrLys: 2.436 ± 0.071
6.985ThrLeu: 6.985 ± 0.127
1.005ThrMet: 1.005 ± 0.04
2.294ThrAsn: 2.294 ± 0.069
2.966ThrPro: 2.966 ± 0.077
2.586ThrGln: 2.586 ± 0.058
2.519ThrArg: 2.519 ± 0.069
3.1ThrSer: 3.1 ± 0.09
3.299ThrThr: 3.299 ± 0.088
3.953ThrVal: 3.953 ± 0.089
0.597ThrTrp: 0.597 ± 0.035
1.623ThrTyr: 1.623 ± 0.054
0.0ThrXaa: 0.0 ± 0.0
Val
5.97ValAla: 5.97 ± 0.101
0.627ValCys: 0.627 ± 0.034
3.813ValAsp: 3.813 ± 0.077
3.793ValGlu: 3.793 ± 0.099
2.862ValPhe: 2.862 ± 0.073
4.777ValGly: 4.777 ± 0.103
1.421ValHis: 1.421 ± 0.049
5.251ValIle: 5.251 ± 0.095
3.841ValLys: 3.841 ± 0.102
7.019ValLeu: 7.019 ± 0.111
1.958ValMet: 1.958 ± 0.056
3.163ValAsn: 3.163 ± 0.074
2.826ValPro: 2.826 ± 0.076
1.985ValGln: 1.985 ± 0.068
3.002ValArg: 3.002 ± 0.067
4.685ValSer: 4.685 ± 0.087
4.171ValThr: 4.171 ± 0.083
5.51ValVal: 5.51 ± 0.125
0.773ValTrp: 0.773 ± 0.047
1.83ValTyr: 1.83 ± 0.057
0.0ValXaa: 0.0 ± 0.0
Trp
0.81TrpAla: 0.81 ± 0.041
0.174TrpCys: 0.174 ± 0.018
0.539TrpAsp: 0.539 ± 0.034
0.397TrpGlu: 0.397 ± 0.028
0.675TrpPhe: 0.675 ± 0.041
0.853TrpGly: 0.853 ± 0.038
0.387TrpHis: 0.387 ± 0.026
0.949TrpIle: 0.949 ± 0.043
0.482TrpLys: 0.482 ± 0.029
1.854TrpLeu: 1.854 ± 0.057
0.302TrpMet: 0.302 ± 0.027
0.451TrpAsn: 0.451 ± 0.03
0.426TrpPro: 0.426 ± 0.026
0.946TrpGln: 0.946 ± 0.045
0.818TrpArg: 0.818 ± 0.041
0.691TrpSer: 0.691 ± 0.038
0.464TrpThr: 0.464 ± 0.031
0.979TrpVal: 0.979 ± 0.041
0.242TrpTrp: 0.242 ± 0.023
0.404TrpTyr: 0.404 ± 0.028
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.312TyrAla: 2.312 ± 0.061
0.369TyrCys: 0.369 ± 0.028
1.445TyrAsp: 1.445 ± 0.054
1.388TyrGlu: 1.388 ± 0.051
1.479TyrPhe: 1.479 ± 0.055
2.148TyrGly: 2.148 ± 0.069
0.832TyrHis: 0.832 ± 0.038
1.737TyrIle: 1.737 ± 0.054
1.198TyrLys: 1.198 ± 0.044
3.446TyrLeu: 3.446 ± 0.077
0.532TyrMet: 0.532 ± 0.031
1.138TyrAsn: 1.138 ± 0.053
1.481TyrPro: 1.481 ± 0.055
1.839TyrGln: 1.839 ± 0.066
1.759TyrArg: 1.759 ± 0.063
1.975TyrSer: 1.975 ± 0.058
1.457TyrThr: 1.457 ± 0.049
1.909TyrVal: 1.909 ± 0.061
0.449TyrTrp: 0.449 ± 0.029
1.003TyrTyr: 1.003 ± 0.044
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 1842 proteins (579268 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski