Raineya orbicola
Average proteome isoelectric point is 7.0
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2684 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2N3IHX5|A0A2N3IHX5_9BACT Phosphoheptose isomerase OS=Raineya orbicola OX=2016530 GN=gmhA PE=3 SV=1
MM1 pKa = 7.54 LSKK4 pKa = 9.64 ITYY7 pKa = 9.09 KK8 pKa = 10.8 KK9 pKa = 10.79 LFGFRR14 pKa = 11.84 QGTGLTPNILGFGTILLLLYY34 pKa = 8.76 WLNVTSSYY42 pKa = 11.21 AQAGKK47 pKa = 10.38 DD48 pKa = 3.23 GALTVTTANTVLNRR62 pKa = 11.84 YY63 pKa = 6.59 TRR65 pKa = 11.84 VTTDD69 pKa = 2.79 VTAGSNVITVVNINDD84 pKa = 3.95 LNRR87 pKa = 11.84 DD88 pKa = 3.78 GIGYY92 pKa = 9.57 LPAGFVTNSSVYY104 pKa = 10.85 ANNALSPGDD113 pKa = 4.61 LIIIYY118 pKa = 8.58 QAQGAIINSTNTINYY133 pKa = 8.06 GQVTNYY139 pKa = 10.27 NGAGTYY145 pKa = 9.77 EE146 pKa = 4.35 FAQVASVSGNNITLACRR163 pKa = 11.84 TKK165 pKa = 10.42 YY166 pKa = 10.7 SYY168 pKa = 10.67 FAARR172 pKa = 11.84 YY173 pKa = 7.33 VQVIRR178 pKa = 11.84 VPQYY182 pKa = 7.4 TTLTVNAGASVVAVPWGAPSFGGADD207 pKa = 3.43 PSALEE212 pKa = 3.86 RR213 pKa = 11.84 RR214 pKa = 11.84 RR215 pKa = 11.84 GGFMVVHH222 pKa = 6.65 ATNIVNNGTIHH233 pKa = 6.95 ANAAGFRR240 pKa = 11.84 GGTIDD245 pKa = 4.92 PNTSPTGNNFSIAFVTNSSNEE266 pKa = 4.01 SAEE269 pKa = 4.03 KK270 pKa = 10.61 GEE272 pKa = 4.58 SIAGYY277 pKa = 10.21 RR278 pKa = 11.84 DD279 pKa = 4.41 DD280 pKa = 5.87 YY281 pKa = 12.03 DD282 pKa = 3.31 NLYY285 pKa = 9.84 NGRR288 pKa = 11.84 YY289 pKa = 9.28 GRR291 pKa = 11.84 GAPANGGGGGNGHH304 pKa = 6.34 NAGGGGGANGGNPAGWFRR322 pKa = 11.84 GAGVMNSFGTCGNPGAWALDD342 pKa = 3.42 PDD344 pKa = 4.65 YY345 pKa = 10.87 IANGNALTNSQGGGRR360 pKa = 11.84 GGYY363 pKa = 10.03 SFGSSNQNACTLGPSYY379 pKa = 9.82 PANFIAPGIPAADD392 pKa = 3.82 VQNTAWGGDD401 pKa = 3.23 NRR403 pKa = 11.84 DD404 pKa = 3.41 AVGGLGGRR412 pKa = 11.84 PIVSANFQNQIFFGGGGGAGDD433 pKa = 4.25 GNNGANADD441 pKa = 3.92 GGDD444 pKa = 3.36 GGGIVFLIATGNITGSGTISANGEE468 pKa = 3.89 NGFNTISGHH477 pKa = 5.7 NDD479 pKa = 2.78 APGGGGGGGTVAIQATSIANTINIRR504 pKa = 11.84 ANGGNGGNQLITNNEE519 pKa = 4.02 SEE521 pKa = 4.54 GPGGGGGGGVIVINATTDD539 pKa = 3.11 GSTKK543 pKa = 10.06 QVNGGQNGTTTSAAVTEE560 pKa = 5.09 FTANGATSGNSGTISSVSVSLFSIVCTSNLGINKK594 pKa = 9.68 AISNSSPNVGTNVTFTLTASNAGPDD619 pKa = 3.44 PATNVVVNDD628 pKa = 4.82 LLPAGYY634 pKa = 7.96 TFVSATPSQGTYY646 pKa = 11.17 NNVTGVWNVGSLGIGGSATLTIVATVNASGPYY678 pKa = 9.39 TNTATISTPDD688 pKa = 3.28 QNDD691 pKa = 3.63 PNTSNNTSSVTPTPVPQTNLGVTKK715 pKa = 9.19 TASNMTPPVGSNITFTITANNAGPSNATGVVVNDD749 pKa = 3.93 VLPSGYY755 pKa = 8.66 TFVSATTSQGTYY767 pKa = 10.52 NNVSGVWSVGALANGASATLTITVTVNASGSYY799 pKa = 11.11 ANTATITGDD808 pKa = 3.19 QADD811 pKa = 4.07 PVAGNNSSTATPTPVPQTNLGVTKK835 pKa = 9.19 TASNMTPPVGSNITFTITANNAGPSNATGVVVNDD869 pKa = 3.93 VLPSGYY875 pKa = 8.85 TFVSATASQGTYY887 pKa = 10.82 NNVSGVWSIGALANGASATLTITVTVNASGSYY919 pKa = 10.16 TNTATISGDD928 pKa = 3.31 QADD931 pKa = 4.22 PVAGNNSSTATPTPVNVAPDD951 pKa = 3.94 AVNDD955 pKa = 3.92 NYY957 pKa = 11.61 TMAQNGNLSNSVATNDD973 pKa = 3.04 ILQAGGNPHH982 pKa = 6.88 TFTLVSGGSAAANGTLTFNPDD1003 pKa = 2.54 GTFTYY1008 pKa = 10.55 VPNTGFSGTVSFTYY1022 pKa = 8.95 QVCNGVTPINQCDD1035 pKa = 3.48 TAVATIVVLPPPVAVNDD1052 pKa = 3.87 NAVTPINTPVSGNLSTNDD1070 pKa = 3.55 NLPSGGTYY1078 pKa = 9.58 TYY1080 pKa = 10.55 TIATPPNPAQGTVTINPDD1098 pKa = 2.67 GTYY1101 pKa = 10.2 TFTPATGFTGTATFTYY1117 pKa = 9.36 QVCNQLGQCSTATVSVLVLPPPVAVNDD1144 pKa = 4.08 TANTPINTPVNGNASTNDD1162 pKa = 3.48 NLPSGGTYY1170 pKa = 9.65 TYY1172 pKa = 10.9 TVTTPPANGSVTMNPDD1188 pKa = 2.51 GTYY1191 pKa = 9.93 TYY1193 pKa = 10.29 TPNTGFTGTDD1203 pKa = 2.69 VFTYY1207 pKa = 7.97 QVCNQVGQCSTATVTINVVPAPVANDD1233 pKa = 3.72 DD1234 pKa = 4.73 SYY1236 pKa = 12.18 TMPQNGSLSNSVVGNDD1252 pKa = 3.35 VLPASGNPHH1261 pKa = 5.85 TFSLVSGGSAAANGTLTFNPDD1282 pKa = 2.54 GTFTYY1287 pKa = 10.55 VPNTGFSGTVSFTYY1301 pKa = 8.96 QVCNSLIPAQCDD1313 pKa = 3.18 TAVATIVVLPPPVAVNDD1330 pKa = 3.87 NAVTPINTPVSGNLSTNDD1348 pKa = 3.55 NLPSGGTYY1356 pKa = 9.58 TYY1358 pKa = 10.55 TIATPPNPAQGTVTINPDD1376 pKa = 2.67 GTYY1379 pKa = 10.2 TFTPATGFTGTATFTYY1395 pKa = 9.36 QVCNQLGQCSTATVSVLVLPPPVAVNDD1422 pKa = 4.08 TANTPINTPVNGNASTNDD1440 pKa = 3.48 NLPSGGTYY1448 pKa = 9.65 TYY1450 pKa = 10.9 TVTTPPANGSVTMNPDD1466 pKa = 2.51 GTYY1469 pKa = 9.93 TYY1471 pKa = 10.29 TPNTGFTGTDD1481 pKa = 2.69 VFTYY1485 pKa = 7.97 QVCNQVGQCSTATVTINVVPAPVANDD1511 pKa = 3.72 DD1512 pKa = 4.73 SYY1514 pKa = 12.18 TMPQNGSLSNSVVGNDD1530 pKa = 3.35 VLPASGNPHH1539 pKa = 5.85 TFSLVSGGSAAANGTLTFNPDD1560 pKa = 2.54 GTFTYY1565 pKa = 10.55 VPNTGFSGTVSFTYY1579 pKa = 8.96 QVCNSLIPAQCDD1591 pKa = 3.18 TAVATIVVLPPPVAVNDD1608 pKa = 4.1 SAVTPINTPVNGNLSSNDD1626 pKa = 3.4 NLPAGGTYY1634 pKa = 9.57 TYY1636 pKa = 10.64 TVATPPNPAQGTVTINPDD1654 pKa = 2.67 GTYY1657 pKa = 10.2 TFTPATGFTGTASFTYY1673 pKa = 8.85 QVCNQLGQCSTATVSVLVLPPPVAVNDD1700 pKa = 4.08 TANTPINTPVNGNASTNDD1718 pKa = 3.48 NLPSGGTYY1726 pKa = 9.65 TYY1728 pKa = 10.9 TVTTPPANGSVTMNPDD1744 pKa = 2.51 GTYY1747 pKa = 9.93 TYY1749 pKa = 10.29 TPNTGFTGTDD1759 pKa = 2.69 VFTYY1763 pKa = 7.97 QVCNQVGQCSTATVTITVPANPVAVNDD1790 pKa = 4.22 NYY1792 pKa = 8.04 TTPINTPVGGNLAANDD1808 pKa = 4.23 LLPTGFTYY1816 pKa = 10.27 TFSLVNGGSAASNGTLVVNPDD1837 pKa = 2.74 GTYY1840 pKa = 11.12 NFTPNTGFSGVVSFTYY1856 pKa = 8.99 QVCNQVNVCSTATVTITILAPPVASNDD1883 pKa = 3.79 TAGTQPNTPVNGNVSPNDD1901 pKa = 3.43 NLPAGGTYY1909 pKa = 9.72 TFTVTTPPANGNVTMNPDD1927 pKa = 2.7 GSYY1930 pKa = 10.06 TYY1932 pKa = 9.88 TPNPGFVGTDD1942 pKa = 2.82 TFTYY1946 pKa = 8.07 TVCNQLGQCSTATVTITVGQPPLAVNDD1973 pKa = 4.25 EE1974 pKa = 4.58 YY1975 pKa = 11.41 NTGAGIPVSGNVGTNDD1991 pKa = 3.43 QNPSGGTTTFNLVSGGTATQNGTLVFNPNGIFTFTPNPGFSGTVTFTYY2039 pKa = 9.0 EE2040 pKa = 3.02 ICNPVGCSQATVTINVAPPVQAVDD2064 pKa = 4.22 DD2065 pKa = 4.82 NYY2067 pKa = 8.9 TTTPNNPVSGNLGTNDD2083 pKa = 3.5 VVPAGQAPTFSLVNGGTATQNGTLVVNPDD2112 pKa = 2.9 GSFTFTPNPGFVGTVTFTYY2131 pKa = 9.49 QVCNQFGQCSTATVTIVIEE2150 pKa = 4.41 DD2151 pKa = 3.92 PVVQIPEE2158 pKa = 4.3 GFSPNNDD2165 pKa = 3.8 GINDD2169 pKa = 3.44 NFVIKK2174 pKa = 10.24 GRR2176 pKa = 11.84 NGRR2179 pKa = 11.84 PVVLRR2184 pKa = 11.84 IYY2186 pKa = 10.41 NRR2188 pKa = 11.84 WGNLVYY2194 pKa = 10.91 EE2195 pKa = 4.34 NLNYY2199 pKa = 9.92 QDD2201 pKa = 4.39 DD2202 pKa = 4.2 WNGTSNIGIRR2212 pKa = 11.84 IGEE2215 pKa = 4.1 QLPDD2219 pKa = 3.07 GTYY2222 pKa = 10.3 FYY2224 pKa = 10.29 TAEE2227 pKa = 4.21 FKK2229 pKa = 10.36 DD2230 pKa = 3.16 TGEE2233 pKa = 3.86 RR2234 pKa = 11.84 YY2235 pKa = 9.83 ARR2237 pKa = 11.84 YY2238 pKa = 8.43 LTIQRR2243 pKa = 4.08
Molecular weight: 227.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.897
IPC2_protein 3.783
IPC_protein 3.859
Toseland 3.605
ProMoST 4.037
Dawson 3.872
Bjellqvist 4.024
Wikipedia 3.846
Rodwell 3.668
Grimsley 3.516
Solomon 3.872
Lehninger 3.821
Nozaki 3.973
DTASelect 4.304
Thurlkill 3.668
EMBOSS 3.846
Sillero 3.986
Patrickios 0.006
IPC_peptide 3.859
IPC2_peptide 3.961
IPC2.peptide.svr19 3.878
Protein with the highest isoelectric point:
>tr|A0A2N3I946|A0A2N3I946_9BACT Outer membrane protein beta-barrel domain OS=Raineya orbicola OX=2016530 GN=Rain11_2252 PE=4 SV=1
MM1 pKa = 7.71 IYY3 pKa = 10.38 SLHH6 pKa = 6.37 KK7 pKa = 10.15 RR8 pKa = 11.84 GASRR12 pKa = 11.84 IVRR15 pKa = 11.84 PFLVANYY22 pKa = 10.01 YY23 pKa = 9.03 NVKK26 pKa = 10.83 GNFATFNWLLLVLALLILFASSVAVGSVSIPFTEE60 pKa = 4.04 IVKK63 pKa = 9.06 TLSGLQEE70 pKa = 3.65 NATWKK75 pKa = 10.4 FIVWEE80 pKa = 3.99 LRR82 pKa = 11.84 LPKK85 pKa = 10.41 ALTAVLVGVALSVAGLVMQTFFANPLASPSEE116 pKa = 4.25 LGVSAGASLGVASITLSSGISWSAFQQWGLVGNSFVAIIAILGAMLVMLLLLLMSRR172 pKa = 11.84 FVWNNTTLLIVGLMLGSLAISLIGLWQFLSSPEE205 pKa = 4.0 QIRR208 pKa = 11.84 DD209 pKa = 3.74 FLWWSFGSLAGTNYY223 pKa = 10.5 SQIAVLAFLVAMSLLLLFLQTQKK246 pKa = 11.34 LNALLLGEE254 pKa = 4.48 MYY256 pKa = 10.61 ARR258 pKa = 11.84 SMGVNIRR265 pKa = 11.84 KK266 pKa = 8.91 LRR268 pKa = 11.84 WVLIVVVSVLSGVTTAFCGPIGFVGLAVPHH298 pKa = 6.8 LARR301 pKa = 11.84 SLFQTANHH309 pKa = 5.64 WTLFPASTLLGASVLLFCDD328 pKa = 3.2 ILAYY332 pKa = 10.99 NLFANFALPINLVTSLLGSPIVIWIVIRR360 pKa = 11.84 RR361 pKa = 11.84 SFRR364 pKa = 11.84 FF365 pKa = 3.22
Molecular weight: 39.69 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.322
IPC2_protein 9.78
IPC_protein 10.467
Toseland 10.438
ProMoST 10.16
Dawson 10.613
Bjellqvist 10.321
Wikipedia 10.818
Rodwell 10.877
Grimsley 10.687
Solomon 10.672
Lehninger 10.628
Nozaki 10.438
DTASelect 10.321
Thurlkill 10.467
EMBOSS 10.833
Sillero 10.526
Patrickios 10.57
IPC_peptide 10.672
IPC2_peptide 9.326
IPC2.peptide.svr19 8.504
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2684
0
2684
933702
29
2470
347.9
39.6
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.918 ± 0.044
0.854 ± 0.014
4.418 ± 0.027
7.171 ± 0.063
5.538 ± 0.04
5.914 ± 0.051
1.727 ± 0.021
7.718 ± 0.046
7.903 ± 0.064
9.86 ± 0.056
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.057 ± 0.019
5.588 ± 0.046
3.644 ± 0.031
4.618 ± 0.031
3.968 ± 0.031
5.655 ± 0.027
5.249 ± 0.048
5.915 ± 0.041
1.229 ± 0.019
4.057 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here