Clerodendrum golden mosaic China virus
Average proteome isoelectric point is 7.9
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|C5J9H3|C5J9H3_9GEMI Capsid protein OS=Clerodendrum golden mosaic China virus OX=559878 GN=AV1 PE=3 SV=1
MM1 pKa = 7.19 LTGHH5 pKa = 6.3 TCMSDD10 pKa = 2.53 MWDD13 pKa = 3.37 PLVNEE18 pKa = 4.72 FPEE21 pKa = 4.71 TVHH24 pKa = 7.1 GLRR27 pKa = 11.84 CMLAVKK33 pKa = 9.45 YY34 pKa = 10.39 LKK36 pKa = 10.56 YY37 pKa = 10.85 VEE39 pKa = 4.33 NTYY42 pKa = 11.14 SPDD45 pKa = 3.18 TLGYY49 pKa = 10.79 DD50 pKa = 4.38 LIRR53 pKa = 11.84 DD54 pKa = 3.93 LVLVIRR60 pKa = 11.84 AKK62 pKa = 10.86 NYY64 pKa = 9.67 GEE66 pKa = 3.55 ATRR69 pKa = 11.84 RR70 pKa = 11.84 YY71 pKa = 8.16 NHH73 pKa = 5.85 FHH75 pKa = 6.76 ARR77 pKa = 11.84 LQGSTEE83 pKa = 3.9 VEE85 pKa = 3.71 LRR87 pKa = 11.84 QPVSEE92 pKa = 4.49 PCACPHH98 pKa = 6.68 CPRR101 pKa = 11.84 HH102 pKa = 5.62 TKK104 pKa = 9.11 TVMGQQAHH112 pKa = 4.99 VSEE115 pKa = 4.78 AQTIPSVPGPP125 pKa = 3.57
Molecular weight: 14.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 6.15
IPC2_protein 6.173
IPC_protein 6.326
Toseland 6.561
ProMoST 6.795
Dawson 6.722
Bjellqvist 6.634
Wikipedia 6.737
Rodwell 6.707
Grimsley 6.678
Solomon 6.737
Lehninger 6.737
Nozaki 6.971
DTASelect 7.132
Thurlkill 7.176
EMBOSS 7.161
Sillero 7.117
Patrickios 3.427
IPC_peptide 6.751
IPC2_peptide 7.029
IPC2.peptide.svr19 6.899
Protein with the highest isoelectric point:
>tr|C5J9H4|C5J9H4_9GEMI Replication enhancer OS=Clerodendrum golden mosaic China virus OX=559878 GN=AC3 PE=3 SV=1
MM1 pKa = 7.9 AKK3 pKa = 10.08 RR4 pKa = 11.84 PGDD7 pKa = 3.87 IIISTPVSKK16 pKa = 10.56 VRR18 pKa = 11.84 RR19 pKa = 11.84 RR20 pKa = 11.84 LNFDD24 pKa = 2.94 SPYY27 pKa = 9.63 QSRR30 pKa = 11.84 VPALTARR37 pKa = 11.84 GTRR40 pKa = 11.84 KK41 pKa = 9.4 QLWANRR47 pKa = 11.84 PMYY50 pKa = 10.08 RR51 pKa = 11.84 KK52 pKa = 8.83 PRR54 pKa = 11.84 LYY56 pKa = 10.31 RR57 pKa = 11.84 VFRR60 pKa = 11.84 GPDD63 pKa = 3.18 VPRR66 pKa = 11.84 GCEE69 pKa = 4.34 GPCKK73 pKa = 10.21 VQSFEE78 pKa = 3.84 QRR80 pKa = 11.84 HH81 pKa = 6.27 DD82 pKa = 3.18 IAHH85 pKa = 6.13 TGKK88 pKa = 10.45 VICISDD94 pKa = 3.51 VTRR97 pKa = 11.84 GNGITHH103 pKa = 6.78 RR104 pKa = 11.84 VGKK107 pKa = 9.44 RR108 pKa = 11.84 FCVKK112 pKa = 9.94 SVYY115 pKa = 10.62 VLGKK119 pKa = 9.71 VWMDD123 pKa = 3.25 EE124 pKa = 4.06 NIKK127 pKa = 9.31 TKK129 pKa = 10.61 NHH131 pKa = 5.92 TNTVMFYY138 pKa = 9.52 VVRR141 pKa = 11.84 DD142 pKa = 3.66 RR143 pKa = 11.84 RR144 pKa = 11.84 PYY146 pKa = 7.97 GTPMDD151 pKa = 4.6 FGQVFNMYY159 pKa = 10.66 DD160 pKa = 3.86 NEE162 pKa = 4.36 PSTATIKK169 pKa = 10.92 NDD171 pKa = 2.93 LRR173 pKa = 11.84 DD174 pKa = 3.55 RR175 pKa = 11.84 FQVIRR180 pKa = 11.84 KK181 pKa = 8.86 FYY183 pKa = 8.92 STVTGGQYY191 pKa = 10.82 ASKK194 pKa = 10.24 EE195 pKa = 3.73 QALVKK200 pKa = 10.66 KK201 pKa = 9.99 FMRR204 pKa = 11.84 VNNHH208 pKa = 4.32 VVYY211 pKa = 10.63 NHH213 pKa = 5.84 QEE215 pKa = 3.45 AAKK218 pKa = 10.4 YY219 pKa = 9.25 EE220 pKa = 4.13 NHH222 pKa = 6.46 TEE224 pKa = 3.99 NALLLYY230 pKa = 9.55 MAGTHH235 pKa = 6.51 ASNPVYY241 pKa = 9.86 ATLKK245 pKa = 9.47 IRR247 pKa = 11.84 IYY249 pKa = 10.67 FYY251 pKa = 11.26 DD252 pKa = 3.51 SVSNN256 pKa = 3.9
Molecular weight: 29.67 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.279
IPC2_protein 9.633
IPC_protein 9.97
Toseland 10.145
ProMoST 9.897
Dawson 10.379
Bjellqvist 10.087
Wikipedia 10.57
Rodwell 10.701
Grimsley 10.452
Solomon 10.394
Lehninger 10.365
Nozaki 10.16
DTASelect 10.072
Thurlkill 10.218
EMBOSS 10.57
Sillero 10.292
Patrickios 10.204
IPC_peptide 10.394
IPC2_peptide 8.946
IPC2.peptide.svr19 8.586
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
8
0
8
1667
96
362
208.4
23.66
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.099 ± 0.522
1.98 ± 0.209
4.799 ± 0.401
4.019 ± 0.606
3.839 ± 0.404
5.699 ± 0.25
3.719 ± 0.438
5.399 ± 0.72
4.679 ± 0.664
6.899 ± 0.588
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.22 ± 0.388
5.939 ± 0.512
5.879 ± 0.367
4.019 ± 0.446
7.499 ± 0.836
8.998 ± 1.219
6.779 ± 0.443
7.079 ± 0.919
1.26 ± 0.136
4.199 ± 0.568
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here