Leucobacter sp. HDW9B

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Microbacteriaceae; Leucobacter

Average proteome isoelectric point is 6.04

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2316 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6G8FGZ1|A0A6G8FGZ1_9MICO TetR/AcrR family transcriptional regulator OS=Leucobacter sp. HDW9B OX=2714934 GN=G7067_00935 PE=4 SV=1
MM1 pKa = 7.49KK2 pKa = 10.38VGSYY6 pKa = 10.63DD7 pKa = 3.38LSEE10 pKa = 4.88AGTAVGYY17 pKa = 8.76EE18 pKa = 3.97AGTWSCDD25 pKa = 3.04GGVLAGSTITLAEE38 pKa = 4.29GEE40 pKa = 4.68KK41 pKa = 10.64VSCTIINTALAPTWTVSKK59 pKa = 10.76SSTPASGSTVLPGTTVTYY77 pKa = 9.79TITATHH83 pKa = 6.49TGGVYY88 pKa = 8.5PTDD91 pKa = 4.2LLITDD96 pKa = 5.05DD97 pKa = 4.96LADD100 pKa = 3.76VLDD103 pKa = 4.02NATLQGEE110 pKa = 4.48PNPSVGTATLGAASSTLDD128 pKa = 3.36WQIDD132 pKa = 4.11TLTGTATLEE141 pKa = 4.0YY142 pKa = 9.85TVLINDD148 pKa = 4.01DD149 pKa = 4.31AFDD152 pKa = 3.81EE153 pKa = 4.46TLHH156 pKa = 6.38NVVTPPVGSSCEE168 pKa = 4.22GSCEE172 pKa = 4.3TTHH175 pKa = 5.59VTPGWRR181 pKa = 11.84LTKK184 pKa = 10.42TSDD187 pKa = 3.49PASGSIVDD195 pKa = 3.95PSSTITYY202 pKa = 7.67TLHH205 pKa = 7.42ALNTSDD211 pKa = 3.84ATVTGATALDD221 pKa = 4.26DD222 pKa = 4.57LSDD225 pKa = 4.01VLDD228 pKa = 4.13EE229 pKa = 5.12ANLVQPLSRR238 pKa = 11.84VV239 pKa = 3.31

Molecular weight: 24.45 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6G8FGI9|A0A6G8FGI9_9MICO Clp protease OS=Leucobacter sp. HDW9B OX=2714934 GN=G7067_02185 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.0RR4 pKa = 11.84TFQPNNRR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84AKK15 pKa = 9.5VHH17 pKa = 5.46GFRR20 pKa = 11.84ARR22 pKa = 11.84MRR24 pKa = 11.84TRR26 pKa = 11.84AGRR29 pKa = 11.84AIVAARR35 pKa = 11.84RR36 pKa = 11.84GKK38 pKa = 10.45GRR40 pKa = 11.84SKK42 pKa = 10.13LTAA45 pKa = 4.04

Molecular weight: 5.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2316

0

2316

716807

29

2441

309.5

33.27

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.486 ± 0.067

0.603 ± 0.014

5.507 ± 0.038

6.243 ± 0.057

3.27 ± 0.03

8.681 ± 0.044

1.91 ± 0.022

4.917 ± 0.037

2.543 ± 0.039

10.148 ± 0.058

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.925 ± 0.022

2.389 ± 0.028

5.188 ± 0.036

3.206 ± 0.027

6.81 ± 0.06

6.222 ± 0.039

6.153 ± 0.062

8.411 ± 0.05

1.384 ± 0.022

2.005 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski