Amino acid dipepetide frequency for Leucobacter sp. HDW9B

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
18.13AlaAla: 18.13 ± 0.255
0.773AlaCys: 0.773 ± 0.034
6.542AlaAsp: 6.542 ± 0.108
8.754AlaGlu: 8.754 ± 0.141
3.627AlaPhe: 3.627 ± 0.077
10.986AlaGly: 10.986 ± 0.141
2.331AlaHis: 2.331 ± 0.056
5.93AlaIle: 5.93 ± 0.098
3.375AlaLys: 3.375 ± 0.1
12.884AlaLeu: 12.884 ± 0.159
2.566AlaMet: 2.566 ± 0.058
2.649AlaAsn: 2.649 ± 0.059
5.858AlaPro: 5.858 ± 0.117
4.22AlaGln: 4.22 ± 0.081
7.998AlaArg: 7.998 ± 0.137
7.229AlaSer: 7.229 ± 0.121
7.083AlaThr: 7.083 ± 0.127
10.105AlaVal: 10.105 ± 0.136
1.617AlaTrp: 1.617 ± 0.053
2.201AlaTyr: 2.201 ± 0.054
0.0AlaXaa: 0.0 ± 0.0
Cys
0.791CysAla: 0.791 ± 0.039
0.073CysCys: 0.073 ± 0.012
0.396CysAsp: 0.396 ± 0.026
0.372CysGlu: 0.372 ± 0.024
0.174CysPhe: 0.174 ± 0.015
0.61CysGly: 0.61 ± 0.033
0.114CysHis: 0.114 ± 0.011
0.18CysIle: 0.18 ± 0.016
0.113CysLys: 0.113 ± 0.011
0.533CysLeu: 0.533 ± 0.025
0.117CysMet: 0.117 ± 0.013
0.14CysAsn: 0.14 ± 0.015
0.299CysPro: 0.299 ± 0.022
0.145CysGln: 0.145 ± 0.013
0.391CysArg: 0.391 ± 0.025
0.382CysSer: 0.382 ± 0.025
0.431CysThr: 0.431 ± 0.026
0.545CysVal: 0.545 ± 0.031
0.1CysTrp: 0.1 ± 0.011
0.124CysTyr: 0.124 ± 0.013
0.0CysXaa: 0.0 ± 0.0
Asp
7.144AspAla: 7.144 ± 0.124
0.253AspCys: 0.253 ± 0.018
2.886AspAsp: 2.886 ± 0.069
3.772AspGlu: 3.772 ± 0.093
1.761AspPhe: 1.761 ± 0.051
4.957AspGly: 4.957 ± 0.1
1.097AspHis: 1.097 ± 0.04
2.327AspIle: 2.327 ± 0.055
1.101AspLys: 1.101 ± 0.052
5.439AspLeu: 5.439 ± 0.094
0.834AspMet: 0.834 ± 0.041
1.127AspAsn: 1.127 ± 0.04
4.494AspPro: 4.494 ± 0.108
1.632AspGln: 1.632 ± 0.052
4.016AspArg: 4.016 ± 0.083
2.84AspSer: 2.84 ± 0.063
3.244AspThr: 3.244 ± 0.07
4.205AspVal: 4.205 ± 0.085
0.773AspTrp: 0.773 ± 0.034
1.17AspTyr: 1.17 ± 0.043
0.0AspXaa: 0.0 ± 0.0
Glu
7.595GluAla: 7.595 ± 0.127
0.34GluCys: 0.34 ± 0.024
3.059GluAsp: 3.059 ± 0.068
3.573GluGlu: 3.573 ± 0.084
2.116GluPhe: 2.116 ± 0.066
4.206GluGly: 4.206 ± 0.091
1.553GluHis: 1.553 ± 0.059
3.273GluIle: 3.273 ± 0.067
1.734GluLys: 1.734 ± 0.06
7.352GluLeu: 7.352 ± 0.123
1.289GluMet: 1.289 ± 0.048
1.528GluAsn: 1.528 ± 0.057
2.754GluPro: 2.754 ± 0.061
2.628GluGln: 2.628 ± 0.071
5.054GluArg: 5.054 ± 0.121
3.537GluSer: 3.537 ± 0.063
3.468GluThr: 3.468 ± 0.074
5.075GluVal: 5.075 ± 0.086
0.97GluTrp: 0.97 ± 0.04
1.325GluTyr: 1.325 ± 0.047
0.0GluXaa: 0.0 ± 0.0
Phe
4.358PheAla: 4.358 ± 0.093
0.215PheCys: 0.215 ± 0.017
2.222PheAsp: 2.222 ± 0.057
2.077PheGlu: 2.077 ± 0.052
1.12PhePhe: 1.12 ± 0.046
3.44PheGly: 3.44 ± 0.076
0.511PheHis: 0.511 ± 0.026
1.328PheIle: 1.328 ± 0.046
0.645PheLys: 0.645 ± 0.036
2.853PheLeu: 2.853 ± 0.069
0.6PheMet: 0.6 ± 0.028
0.766PheAsn: 0.766 ± 0.035
1.542PhePro: 1.542 ± 0.051
0.88PheGln: 0.88 ± 0.037
1.696PheArg: 1.696 ± 0.048
2.079PheSer: 2.079 ± 0.057
2.372PheThr: 2.372 ± 0.069
2.914PheVal: 2.914 ± 0.07
0.438PheTrp: 0.438 ± 0.027
0.647PheTyr: 0.647 ± 0.031
0.0PheXaa: 0.0 ± 0.0
Gly
9.697GlyAla: 9.697 ± 0.132
0.597GlyCys: 0.597 ± 0.027
4.612GlyAsp: 4.612 ± 0.089
5.272GlyGlu: 5.272 ± 0.087
3.33GlyPhe: 3.33 ± 0.061
7.532GlyGly: 7.532 ± 0.151
1.606GlyHis: 1.606 ± 0.052
5.103GlyIle: 5.103 ± 0.085
2.62GlyLys: 2.62 ± 0.071
8.387GlyLeu: 8.387 ± 0.128
1.868GlyMet: 1.868 ± 0.057
2.158GlyAsn: 2.158 ± 0.079
3.271GlyPro: 3.271 ± 0.071
2.55GlyGln: 2.55 ± 0.065
5.205GlyArg: 5.205 ± 0.105
6.138GlySer: 6.138 ± 0.119
5.611GlyThr: 5.611 ± 0.144
7.644GlyVal: 7.644 ± 0.106
1.405GlyTrp: 1.405 ± 0.047
2.204GlyTyr: 2.204 ± 0.06
0.0GlyXaa: 0.0 ± 0.0
His
2.16HisAla: 2.16 ± 0.059
0.131HisCys: 0.131 ± 0.013
1.039HisAsp: 1.039 ± 0.039
1.258HisGlu: 1.258 ± 0.049
0.593HisPhe: 0.593 ± 0.026
1.782HisGly: 1.782 ± 0.052
0.518HisHis: 0.518 ± 0.028
0.73HisIle: 0.73 ± 0.032
0.322HisLys: 0.322 ± 0.022
1.987HisLeu: 1.987 ± 0.055
0.35HisMet: 0.35 ± 0.018
0.407HisAsn: 0.407 ± 0.026
1.353HisPro: 1.353 ± 0.045
0.529HisGln: 0.529 ± 0.029
1.426HisArg: 1.426 ± 0.044
1.15HisSer: 1.15 ± 0.039
1.198HisThr: 1.198 ± 0.046
1.415HisVal: 1.415 ± 0.047
0.299HisTrp: 0.299 ± 0.022
0.451HisTyr: 0.451 ± 0.027
0.0HisXaa: 0.0 ± 0.0
Ile
7.164IleAla: 7.164 ± 0.104
0.311IleCys: 0.311 ± 0.02
3.384IleAsp: 3.384 ± 0.076
3.472IleGlu: 3.472 ± 0.083
1.334IlePhe: 1.334 ± 0.044
4.526IleGly: 4.526 ± 0.091
0.744IleHis: 0.744 ± 0.032
2.13IleIle: 2.13 ± 0.062
1.002IleLys: 1.002 ± 0.04
4.329IleLeu: 4.329 ± 0.084
0.765IleMet: 0.765 ± 0.037
1.189IleAsn: 1.189 ± 0.045
2.666IlePro: 2.666 ± 0.063
1.094IleGln: 1.094 ± 0.036
2.85IleArg: 2.85 ± 0.068
2.99IleSer: 2.99 ± 0.073
3.334IleThr: 3.334 ± 0.077
4.421IleVal: 4.421 ± 0.086
0.59IleTrp: 0.59 ± 0.031
0.873IleTyr: 0.873 ± 0.038
0.0IleXaa: 0.0 ± 0.0
Lys
2.688LysAla: 2.688 ± 0.079
0.105LysCys: 0.105 ± 0.011
1.297LysAsp: 1.297 ± 0.054
1.162LysGlu: 1.162 ± 0.053
0.707LysPhe: 0.707 ± 0.032
1.769LysGly: 1.769 ± 0.056
0.598LysHis: 0.598 ± 0.032
1.433LysIle: 1.433 ± 0.05
1.098LysLys: 1.098 ± 0.053
2.676LysLeu: 2.676 ± 0.075
0.543LysMet: 0.543 ± 0.029
0.759LysAsn: 0.759 ± 0.034
1.556LysPro: 1.556 ± 0.049
0.971LysGln: 0.971 ± 0.039
2.129LysArg: 2.129 ± 0.065
1.491LysSer: 1.491 ± 0.047
1.606LysThr: 1.606 ± 0.057
1.996LysVal: 1.996 ± 0.068
0.299LysTrp: 0.299 ± 0.02
0.547LysTyr: 0.547 ± 0.032
0.0LysXaa: 0.0 ± 0.0
Leu
13.383LeuAla: 13.383 ± 0.177
0.625LeuCys: 0.625 ± 0.034
5.773LeuAsp: 5.773 ± 0.102
5.657LeuGlu: 5.657 ± 0.107
2.923LeuPhe: 2.923 ± 0.076
9.361LeuGly: 9.361 ± 0.14
1.773LeuHis: 1.773 ± 0.054
4.912LeuIle: 4.912 ± 0.09
2.402LeuLys: 2.402 ± 0.071
10.308LeuLeu: 10.308 ± 0.179
1.782LeuMet: 1.782 ± 0.048
2.356LeuAsn: 2.356 ± 0.056
5.435LeuPro: 5.435 ± 0.092
2.942LeuGln: 2.942 ± 0.07
7.578LeuArg: 7.578 ± 0.129
6.606LeuSer: 6.606 ± 0.108
6.399LeuThr: 6.399 ± 0.11
8.432LeuVal: 8.432 ± 0.138
1.207LeuTrp: 1.207 ± 0.044
1.621LeuTyr: 1.621 ± 0.048
0.0LeuXaa: 0.0 ± 0.0
Met
2.021MetAla: 2.021 ± 0.054
0.106MetCys: 0.106 ± 0.013
0.797MetAsp: 0.797 ± 0.038
0.735MetGlu: 0.735 ± 0.029
0.618MetPhe: 0.618 ± 0.03
1.31MetGly: 1.31 ± 0.044
0.368MetHis: 0.368 ± 0.024
1.038MetIle: 1.038 ± 0.039
0.58MetLys: 0.58 ± 0.025
2.144MetLeu: 2.144 ± 0.063
0.381MetMet: 0.381 ± 0.023
0.664MetAsn: 0.664 ± 0.031
1.193MetPro: 1.193 ± 0.041
0.649MetGln: 0.649 ± 0.033
1.472MetArg: 1.472 ± 0.043
1.713MetSer: 1.713 ± 0.046
1.554MetThr: 1.554 ± 0.044
1.395MetVal: 1.395 ± 0.047
0.204MetTrp: 0.204 ± 0.018
0.311MetTyr: 0.311 ± 0.021
0.0MetXaa: 0.0 ± 0.0
Asn
2.806AsnAla: 2.806 ± 0.078
0.138AsnCys: 0.138 ± 0.016
1.314AsnAsp: 1.314 ± 0.048
1.392AsnGlu: 1.392 ± 0.045
0.738AsnPhe: 0.738 ± 0.035
2.327AsnGly: 2.327 ± 0.073
0.402AsnHis: 0.402 ± 0.024
1.018AsnIle: 1.018 ± 0.036
0.572AsnLys: 0.572 ± 0.027
2.196AsnLeu: 2.196 ± 0.056
0.425AsnMet: 0.425 ± 0.024
0.649AsnAsn: 0.649 ± 0.033
1.8AsnPro: 1.8 ± 0.066
0.769AsnGln: 0.769 ± 0.031
1.535AsnArg: 1.535 ± 0.045
1.36AsnSer: 1.36 ± 0.046
1.606AsnThr: 1.606 ± 0.051
1.879AsnVal: 1.879 ± 0.062
0.378AsnTrp: 0.378 ± 0.024
0.586AsnTyr: 0.586 ± 0.033
0.0AsnXaa: 0.0 ± 0.0
Pro
6.494ProAla: 6.494 ± 0.127
0.209ProCys: 0.209 ± 0.018
3.117ProAsp: 3.117 ± 0.082
4.494ProGlu: 4.494 ± 0.091
1.628ProPhe: 1.628 ± 0.047
5.155ProGly: 5.155 ± 0.094
0.982ProHis: 0.982 ± 0.037
2.273ProIle: 2.273 ± 0.052
1.441ProLys: 1.441 ± 0.043
4.787ProLeu: 4.787 ± 0.099
0.919ProMet: 0.919 ± 0.039
1.313ProAsn: 1.313 ± 0.045
1.907ProPro: 1.907 ± 0.07
1.726ProGln: 1.726 ± 0.055
3.115ProArg: 3.115 ± 0.078
3.181ProSer: 3.181 ± 0.072
2.892ProThr: 2.892 ± 0.07
4.579ProVal: 4.579 ± 0.097
0.699ProTrp: 0.699 ± 0.027
0.971ProTyr: 0.971 ± 0.035
0.0ProXaa: 0.0 ± 0.0
Gln
3.763GlnAla: 3.763 ± 0.077
0.14GlnCys: 0.14 ± 0.013
1.539GlnAsp: 1.539 ± 0.041
1.597GlnGlu: 1.597 ± 0.053
1.057GlnPhe: 1.057 ± 0.041
2.246GlnGly: 2.246 ± 0.061
0.752GlnHis: 0.752 ± 0.035
1.761GlnIle: 1.761 ± 0.052
0.893GlnLys: 0.893 ± 0.041
3.763GlnLeu: 3.763 ± 0.075
0.723GlnMet: 0.723 ± 0.033
0.83GlnAsn: 0.83 ± 0.036
1.606GlnPro: 1.606 ± 0.045
1.504GlnGln: 1.504 ± 0.053
2.69GlnArg: 2.69 ± 0.07
1.897GlnSer: 1.897 ± 0.049
1.748GlnThr: 1.748 ± 0.06
2.43GlnVal: 2.43 ± 0.06
0.431GlnTrp: 0.431 ± 0.027
0.688GlnTyr: 0.688 ± 0.032
0.0GlnXaa: 0.0 ± 0.0
Arg
7.98ArgAla: 7.98 ± 0.137
0.419ArgCys: 0.419 ± 0.031
3.937ArgAsp: 3.937 ± 0.088
4.784ArgGlu: 4.784 ± 0.103
2.434ArgPhe: 2.434 ± 0.066
5.304ArgGly: 5.304 ± 0.094
1.282ArgHis: 1.282 ± 0.04
3.796ArgIle: 3.796 ± 0.071
1.805ArgLys: 1.805 ± 0.052
6.804ArgLeu: 6.804 ± 0.118
1.482ArgMet: 1.482 ± 0.042
1.536ArgAsn: 1.536 ± 0.049
2.942ArgPro: 2.942 ± 0.072
2.153ArgGln: 2.153 ± 0.063
5.488ArgArg: 5.488 ± 0.109
4.127ArgSer: 4.127 ± 0.079
3.647ArgThr: 3.647 ± 0.083
5.741ArgVal: 5.741 ± 0.112
0.985ArgTrp: 0.985 ± 0.036
1.451ArgTyr: 1.451 ± 0.049
0.0ArgXaa: 0.0 ± 0.0
Ser
7.381SerAla: 7.381 ± 0.116
0.446SerCys: 0.446 ± 0.023
3.461SerAsp: 3.461 ± 0.081
3.825SerGlu: 3.825 ± 0.077
2.252SerPhe: 2.252 ± 0.068
6.542SerGly: 6.542 ± 0.112
1.14SerHis: 1.14 ± 0.039
2.928SerIle: 2.928 ± 0.067
1.547SerLys: 1.547 ± 0.048
6.018SerLeu: 6.018 ± 0.088
1.3SerMet: 1.3 ± 0.041
1.532SerAsn: 1.532 ± 0.055
3.178SerPro: 3.178 ± 0.071
1.867SerGln: 1.867 ± 0.054
3.93SerArg: 3.93 ± 0.08
3.733SerSer: 3.733 ± 0.084
3.714SerThr: 3.714 ± 0.089
4.98SerVal: 4.98 ± 0.087
0.981SerTrp: 0.981 ± 0.034
1.461SerTyr: 1.461 ± 0.051
0.0SerXaa: 0.0 ± 0.0
Thr
7.379ThrAla: 7.379 ± 0.137
0.332ThrCys: 0.332 ± 0.023
3.111ThrAsp: 3.111 ± 0.068
3.599ThrGlu: 3.599 ± 0.076
1.907ThrPhe: 1.907 ± 0.058
5.703ThrGly: 5.703 ± 0.099
1.228ThrHis: 1.228 ± 0.039
3.069ThrIle: 3.069 ± 0.086
1.413ThrLys: 1.413 ± 0.046
6.497ThrLeu: 6.497 ± 0.11
1.055ThrMet: 1.055 ± 0.033
1.451ThrAsn: 1.451 ± 0.055
4.014ThrPro: 4.014 ± 0.084
1.982ThrGln: 1.982 ± 0.05
3.496ThrArg: 3.496 ± 0.074
3.804ThrSer: 3.804 ± 0.094
3.564ThrThr: 3.564 ± 0.1
6.062ThrVal: 6.062 ± 0.153
0.763ThrTrp: 0.763 ± 0.033
1.102ThrTyr: 1.102 ± 0.053
0.0ThrXaa: 0.0 ± 0.0
Val
10.149ValAla: 10.149 ± 0.145
0.547ValCys: 0.547 ± 0.026
4.692ValAsp: 4.692 ± 0.084
4.771ValGlu: 4.771 ± 0.082
3.073ValPhe: 3.073 ± 0.071
6.218ValGly: 6.218 ± 0.093
1.514ValHis: 1.514 ± 0.046
4.411ValIle: 4.411 ± 0.078
2.109ValLys: 2.109 ± 0.068
8.743ValLeu: 8.743 ± 0.141
1.589ValMet: 1.589 ± 0.048
1.963ValAsn: 1.963 ± 0.058
4.371ValPro: 4.371 ± 0.08
2.571ValGln: 2.571 ± 0.064
5.317ValArg: 5.317 ± 0.094
5.762ValSer: 5.762 ± 0.107
6.025ValThr: 6.025 ± 0.137
7.6ValVal: 7.6 ± 0.128
1.136ValTrp: 1.136 ± 0.044
1.55ValTyr: 1.55 ± 0.047
0.0ValXaa: 0.0 ± 0.0
Trp
1.48TrpAla: 1.48 ± 0.049
0.128TrpCys: 0.128 ± 0.014
0.696TrpAsp: 0.696 ± 0.031
0.654TrpGlu: 0.654 ± 0.028
0.548TrpPhe: 0.548 ± 0.03
1.066TrpGly: 1.066 ± 0.045
0.265TrpHis: 0.265 ± 0.018
0.762TrpIle: 0.762 ± 0.035
0.317TrpLys: 0.317 ± 0.021
1.526TrpLeu: 1.526 ± 0.054
0.349TrpMet: 0.349 ± 0.024
0.409TrpAsn: 0.409 ± 0.028
0.621TrpPro: 0.621 ± 0.033
0.569TrpGln: 0.569 ± 0.033
1.076TrpArg: 1.076 ± 0.038
0.944TrpSer: 0.944 ± 0.033
0.707TrpThr: 0.707 ± 0.032
1.143TrpVal: 1.143 ± 0.047
0.3TrpTrp: 0.3 ± 0.023
0.282TrpTyr: 0.282 ± 0.02
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.295TyrAla: 2.295 ± 0.055
0.144TyrCys: 0.144 ± 0.013
1.197TyrAsp: 1.197 ± 0.039
1.207TyrGlu: 1.207 ± 0.041
0.762TyrPhe: 0.762 ± 0.035
1.775TyrGly: 1.775 ± 0.048
0.319TyrHis: 0.319 ± 0.021
0.765TyrIle: 0.765 ± 0.036
0.34TyrLys: 0.34 ± 0.021
2.25TyrLeu: 2.25 ± 0.062
0.317TyrMet: 0.317 ± 0.021
0.465TyrAsn: 0.465 ± 0.025
1.023TyrPro: 1.023 ± 0.043
0.675TyrGln: 0.675 ± 0.032
1.634TyrArg: 1.634 ± 0.052
1.253TyrSer: 1.253 ± 0.047
1.328TyrThr: 1.328 ± 0.055
1.55TyrVal: 1.55 ± 0.049
0.269TyrTrp: 0.269 ± 0.019
0.487TyrTyr: 0.487 ± 0.033
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 2316 proteins (716807 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski