Corynebacterium maris DSM 45190
Average proteome isoelectric point is 5.69
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2584 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|S5SV54|S5SV54_9CORY Exopolyphosphatase OS=Corynebacterium maris DSM 45190 OX=1224163 GN=B841_07980 PE=4 SV=1
MM1 pKa = 7.51 SPDD4 pKa = 3.25 QSRR7 pKa = 11.84 TSPPASARR15 pKa = 11.84 NRR17 pKa = 11.84 TGLLWSIAAVLAVLIVGLVIAIIALQTSGNSDD49 pKa = 3.18 VTQADD54 pKa = 4.5 DD55 pKa = 3.48 LTEE58 pKa = 3.9 THH60 pKa = 5.96 VAEE63 pKa = 4.33 EE64 pKa = 4.51 TVVVTDD70 pKa = 3.85 TTAADD75 pKa = 3.5 PTEE78 pKa = 4.83 PGEE81 pKa = 4.38 STPPAATTSTTQGEE95 pKa = 4.55 TRR97 pKa = 11.84 DD98 pKa = 3.67 DD99 pKa = 3.5 ATEE102 pKa = 3.75 VRR104 pKa = 11.84 RR105 pKa = 11.84 ISPVTDD111 pKa = 2.93 DD112 pKa = 3.26 WLPRR116 pKa = 11.84 DD117 pKa = 3.31 GWTVTNTTEE126 pKa = 4.02 MMQGDD131 pKa = 5.65 CYY133 pKa = 10.18 PSPVALDD140 pKa = 3.62 DD141 pKa = 4.63 GIYY144 pKa = 10.23 RR145 pKa = 11.84 CGPSAMSANACFHH158 pKa = 6.43 NPDD161 pKa = 3.71 SGVFYY166 pKa = 10.98 CPFNPFTPEE175 pKa = 3.04 FRR177 pKa = 11.84 AYY179 pKa = 10.35 YY180 pKa = 8.96 FAGEE184 pKa = 4.13 ITHH187 pKa = 6.43 TPVIDD192 pKa = 5.55 DD193 pKa = 3.83 PMPWGLEE200 pKa = 3.77 LDD202 pKa = 4.36 DD203 pKa = 5.92 ARR205 pKa = 11.84 QCTARR210 pKa = 11.84 QGGAWGWRR218 pKa = 11.84 ADD220 pKa = 4.28 DD221 pKa = 3.68 YY222 pKa = 11.84 VGAYY226 pKa = 9.68 SCGGGSDD233 pKa = 3.86 EE234 pKa = 5.96 VILALPGEE242 pKa = 4.34 PVVDD246 pKa = 4.96 DD247 pKa = 5.91 SDD249 pKa = 5.24 DD250 pKa = 3.25 KK251 pKa = 11.01 WAVLMGEE258 pKa = 4.4 MGQGEE263 pKa = 5.46 DD264 pKa = 4.6 FPAPQPVGVAVAYY277 pKa = 9.34 YY278 pKa = 10.6 AGWQDD283 pKa = 3.06
Molecular weight: 30.25 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.689
IPC2_protein 3.795
IPC_protein 3.808
Toseland 3.592
ProMoST 3.948
Dawson 3.808
Bjellqvist 3.986
Wikipedia 3.745
Rodwell 3.63
Grimsley 3.49
Solomon 3.795
Lehninger 3.757
Nozaki 3.923
DTASelect 4.177
Thurlkill 3.643
EMBOSS 3.757
Sillero 3.935
Patrickios 1.214
IPC_peptide 3.795
IPC2_peptide 3.91
IPC2.peptide.svr19 3.833
Protein with the highest isoelectric point:
>tr|S5SR58|S5SR58_9CORY Short chain dehydrogenase OS=Corynebacterium maris DSM 45190 OX=1224163 GN=B841_00405 PE=3 SV=1
MM1 pKa = 7.7 AKK3 pKa = 10.02 GKK5 pKa = 8.53 KK6 pKa = 7.65 TFQPNNRR13 pKa = 11.84 RR14 pKa = 11.84 RR15 pKa = 11.84 SRR17 pKa = 11.84 KK18 pKa = 8.53 HH19 pKa = 4.82 GFRR22 pKa = 11.84 TRR24 pKa = 11.84 MRR26 pKa = 11.84 TRR28 pKa = 11.84 AGRR31 pKa = 11.84 AIVSARR37 pKa = 11.84 RR38 pKa = 11.84 RR39 pKa = 11.84 KK40 pKa = 9.65 GRR42 pKa = 11.84 ASVTAA47 pKa = 4.08
Molecular weight: 5.47 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.316
IPC_protein 12.925
Toseland 13.086
ProMoST 13.583
Dawson 13.086
Bjellqvist 13.086
Wikipedia 13.568
Rodwell 12.735
Grimsley 13.13
Solomon 13.583
Lehninger 13.481
Nozaki 13.086
DTASelect 13.086
Thurlkill 13.086
EMBOSS 13.583
Sillero 13.086
Patrickios 12.457
IPC_peptide 13.583
IPC2_peptide 12.574
IPC2.peptide.svr19 9.237
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2584
0
2584
833534
38
3013
322.6
34.82
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.31 ± 0.056
0.568 ± 0.011
6.629 ± 0.039
6.467 ± 0.05
3.145 ± 0.029
8.604 ± 0.043
2.199 ± 0.022
4.425 ± 0.035
2.529 ± 0.036
9.786 ± 0.056
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.161 ± 0.021
2.442 ± 0.029
5.189 ± 0.037
3.15 ± 0.027
6.544 ± 0.042
5.349 ± 0.028
6.184 ± 0.028
8.683 ± 0.044
1.457 ± 0.021
2.18 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here