Hubei yanvirus-like virus 1

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified RNA viruses ShiM-2016

Average proteome isoelectric point is 7.09

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L3KLE7|A0A1L3KLE7_9VIRU RNA-dependent RNA polymerase OS=Hubei yanvirus-like virus 1 OX=1923343 PE=4 SV=1
MM1 pKa = 7.19QVWEE5 pKa = 5.43WICTMPSRR13 pKa = 11.84RR14 pKa = 11.84RR15 pKa = 11.84KK16 pKa = 8.94QLVRR20 pKa = 11.84AFKK23 pKa = 10.62ARR25 pKa = 11.84EE26 pKa = 3.86EE27 pKa = 4.19LGEE30 pKa = 4.51DD31 pKa = 3.32SPEE34 pKa = 3.9YY35 pKa = 10.34EE36 pKa = 4.15IIKK39 pKa = 10.57AFVKK43 pKa = 10.28TEE45 pKa = 3.78LLPYY49 pKa = 10.37FGVADD54 pKa = 3.57GDD56 pKa = 3.89YY57 pKa = 11.19SVFQVEE63 pKa = 4.12YY64 pKa = 9.7VARR67 pKa = 11.84LIQAPHH73 pKa = 7.32DD74 pKa = 4.05EE75 pKa = 4.44THH77 pKa = 6.99LDD79 pKa = 3.22AGPYY83 pKa = 9.67LKK85 pKa = 10.59PLVQRR90 pKa = 11.84LKK92 pKa = 9.88EE93 pKa = 4.15TWSWEE98 pKa = 3.38HH99 pKa = 5.24WLFYY103 pKa = 11.04ASVSPEE109 pKa = 4.79KK110 pKa = 10.45LDD112 pKa = 3.09KK113 pKa = 10.21WLNKK117 pKa = 9.58HH118 pKa = 6.43RR119 pKa = 11.84GATSWFWSDD128 pKa = 3.42YY129 pKa = 11.09SSFDD133 pKa = 3.32STYY136 pKa = 10.97SAHH139 pKa = 5.11TWRR142 pKa = 11.84MIEE145 pKa = 4.23RR146 pKa = 11.84FYY148 pKa = 10.65QQIYY152 pKa = 9.72PEE154 pKa = 4.56APEE157 pKa = 4.5SLWKK161 pKa = 10.53ALNAWRR167 pKa = 11.84KK168 pKa = 7.37PHH170 pKa = 5.82GKK172 pKa = 9.27VRR174 pKa = 11.84SHH176 pKa = 7.22RR177 pKa = 11.84DD178 pKa = 3.42DD179 pKa = 4.45AKK181 pKa = 11.16LEE183 pKa = 4.02YY184 pKa = 9.84FAPIMNASGRR194 pKa = 11.84DD195 pKa = 3.49DD196 pKa = 3.48TALANALVNGIVLALSFTAVLAGKK220 pKa = 9.96SIEE223 pKa = 4.28SLEE226 pKa = 4.16EE227 pKa = 4.78ADD229 pKa = 4.11VLWASRR235 pKa = 11.84QFSIAVVGDD244 pKa = 4.01DD245 pKa = 4.54SLVACSFDD253 pKa = 3.34VRR255 pKa = 11.84PLVGAIEE262 pKa = 4.21GKK264 pKa = 9.39IRR266 pKa = 11.84RR267 pKa = 11.84FGLVAKK273 pKa = 9.64CFSSEE278 pKa = 4.13EE279 pKa = 4.19LCDD282 pKa = 3.67VTFLGMMPYY291 pKa = 9.95PVAGDD296 pKa = 4.44LYY298 pKa = 9.12WGPTIGRR305 pKa = 11.84RR306 pKa = 11.84LYY308 pKa = 10.59KK309 pKa = 10.49AFWQADD315 pKa = 3.66PKK317 pKa = 11.4GNLPAWTLGVAKK329 pKa = 10.27QLALYY334 pKa = 9.84QCVPVLSEE342 pKa = 3.49IANRR346 pKa = 11.84VCTLLDD352 pKa = 3.5GGKK355 pKa = 8.19ITRR358 pKa = 11.84QAVDD362 pKa = 3.46EE363 pKa = 4.14NRR365 pKa = 11.84VWSARR370 pKa = 11.84SSATPPYY377 pKa = 10.27DD378 pKa = 3.49YY379 pKa = 11.03STMVWMCKK387 pKa = 8.91RR388 pKa = 11.84YY389 pKa = 9.67RR390 pKa = 11.84EE391 pKa = 4.08QGLVPRR397 pKa = 11.84AMAADD402 pKa = 3.72VEE404 pKa = 4.72TVEE407 pKa = 5.72RR408 pKa = 11.84IDD410 pKa = 3.59RR411 pKa = 11.84LPAIVRR417 pKa = 11.84LLTVDD422 pKa = 3.87ASLLVDD428 pKa = 4.52DD429 pKa = 5.53LL430 pKa = 4.71

Molecular weight: 49.03 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L3KLE7|A0A1L3KLE7_9VIRU RNA-dependent RNA polymerase OS=Hubei yanvirus-like virus 1 OX=1923343 PE=4 SV=1
MM1 pKa = 7.8ISEE4 pKa = 4.6PFWLASLCFSTTTAEE19 pKa = 4.08MPLTKK24 pKa = 10.15VRR26 pKa = 11.84NATSGVSDD34 pKa = 3.47IAKK37 pKa = 10.32AIAIPQEE44 pKa = 3.91HH45 pKa = 6.43RR46 pKa = 11.84PIRR49 pKa = 11.84IPTYY53 pKa = 9.77PSLEE57 pKa = 4.02RR58 pKa = 11.84SAVLHH63 pKa = 5.72LQQTRR68 pKa = 11.84TLKK71 pKa = 10.78VGAAGYY77 pKa = 9.89FDD79 pKa = 5.32AMLFRR84 pKa = 11.84SPVHH88 pKa = 5.99PLWGTYY94 pKa = 8.28QPSTSNAAVYY104 pKa = 9.79CSWNYY109 pKa = 7.79TASTILTTTTSMPINAGVFGTPQGVGVGGPDD140 pKa = 3.08WAAVTPVGYY149 pKa = 10.63GKK151 pKa = 10.34DD152 pKa = 3.75GLPYY156 pKa = 9.46MYY158 pKa = 10.21IPANVLFQATLNSTATFTTWEE179 pKa = 4.24MDD181 pKa = 3.46LEE183 pKa = 4.65CYY185 pKa = 10.62YY186 pKa = 10.66GTDD189 pKa = 3.59DD190 pKa = 3.62SSEE193 pKa = 4.15RR194 pKa = 11.84LSIMLTGSGVFGNGLSINANAWVRR218 pKa = 11.84PVALRR223 pKa = 11.84FTAAPTVAGNLTYY236 pKa = 11.31VNIGFFGGGSFNAPNAPIVASFFPLFPVPEE266 pKa = 4.26IQNSVTPYY274 pKa = 9.99YY275 pKa = 9.64STRR278 pKa = 11.84CTAAAVLFSNVTAVLQKK295 pKa = 10.68EE296 pKa = 4.6GTVQAGRR303 pKa = 11.84LNVSSGYY310 pKa = 9.91PYY312 pKa = 10.88DD313 pKa = 3.79PSFSFGVLTSLHH325 pKa = 5.73PQEE328 pKa = 5.36KK329 pKa = 10.11YY330 pKa = 10.15FGKK333 pKa = 10.86LEE335 pKa = 4.69DD336 pKa = 3.57GLYY339 pKa = 10.84AFSLPSAEE347 pKa = 3.9SDD349 pKa = 3.5TFRR352 pKa = 11.84DD353 pKa = 4.26CVSLSAIASKK363 pKa = 10.25PFPLFHH369 pKa = 7.53IEE371 pKa = 3.88GFGYY375 pKa = 7.98YY376 pKa = 7.75QHH378 pKa = 6.49MRR380 pKa = 11.84FSDD383 pKa = 4.1LDD385 pKa = 3.63ATSATQLAITLDD397 pKa = 3.4YY398 pKa = 10.72HH399 pKa = 8.15LEE401 pKa = 4.04FRR403 pKa = 11.84SSSMLFNLGYY413 pKa = 10.55SSLPLEE419 pKa = 5.07AYY421 pKa = 10.39HH422 pKa = 6.37EE423 pKa = 4.43AQLALVKK430 pKa = 10.29MGTFFEE436 pKa = 4.47NPKK439 pKa = 10.19HH440 pKa = 6.38ISQITKK446 pKa = 10.06KK447 pKa = 10.56VLNSLPKK454 pKa = 9.41VRR456 pKa = 11.84APALVVMKK464 pKa = 8.43VTKK467 pKa = 10.47GAGRR471 pKa = 11.84PDD473 pKa = 3.51GSKK476 pKa = 10.73GGKK479 pKa = 8.94KK480 pKa = 10.3DD481 pKa = 3.65NAPKK485 pKa = 10.49QNGGKK490 pKa = 9.92GKK492 pKa = 10.18KK493 pKa = 9.13SKK495 pKa = 10.24KK496 pKa = 10.52GKK498 pKa = 9.75DD499 pKa = 3.06KK500 pKa = 11.03KK501 pKa = 10.95

Molecular weight: 54.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

1648

430

717

549.3

60.37

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.83 ± 0.162

1.881 ± 0.419

4.187 ± 0.641

5.34 ± 0.689

3.883 ± 0.757

7.403 ± 0.697

1.881 ± 0.113

2.973 ± 0.602

4.551 ± 0.421

8.859 ± 0.362

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.699 ± 0.304

2.973 ± 0.448

7.767 ± 1.151

3.58 ± 0.386

5.583 ± 0.839

7.646 ± 0.547

6.25 ± 0.75

7.706 ± 0.403

2.549 ± 0.519

3.459 ± 0.567
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski