Pseudorhodobacter sp. MZDSW-24AT

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Rhodobacteraceae; Pseudorhodobacter; unclassified Pseudorhodobacter

Average proteome isoelectric point is 6.5

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3602 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2M8N2B4|A0A2M8N2B4_9RHOB Uncharacterized protein OS=Pseudorhodobacter sp. MZDSW-24AT OX=2052957 GN=CUR21_04575 PE=4 SV=1
MM1 pKa = 7.76ADD3 pKa = 4.09AYY5 pKa = 11.43NSTTTTTTPGYY16 pKa = 8.25TNPANPPHH24 pKa = 6.63GAATHH29 pKa = 4.93TTHH32 pKa = 6.4VRR34 pKa = 11.84EE35 pKa = 4.37THH37 pKa = 6.61QSTNWFAWLLGGAVVAVGIGALVYY61 pKa = 11.08SNDD64 pKa = 3.54DD65 pKa = 3.62VAPGTPAAVDD75 pKa = 3.47NNVTIQSPATAPAADD90 pKa = 4.19APVATTPDD98 pKa = 3.23ATAPAADD105 pKa = 4.27APVATTPDD113 pKa = 3.23ATAPAADD120 pKa = 4.39APAAADD126 pKa = 3.6PVAPAPEE133 pKa = 4.39AAPAAPTGNN142 pKa = 3.5

Molecular weight: 13.77 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2M8N1V0|A0A2M8N1V0_9RHOB Autotransporter domain-containing protein OS=Pseudorhodobacter sp. MZDSW-24AT OX=2052957 GN=CUR21_03690 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.44RR3 pKa = 11.84TYY5 pKa = 10.31QPSNLVRR12 pKa = 11.84KK13 pKa = 9.18RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.34GGRR28 pKa = 11.84LVLAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.21GRR39 pKa = 11.84HH40 pKa = 5.51KK41 pKa = 10.9LSAA44 pKa = 3.8

Molecular weight: 5.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3602

0

3602

1155149

23

2375

320.7

34.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.659 ± 0.056

0.859 ± 0.012

5.538 ± 0.034

5.356 ± 0.037

3.638 ± 0.028

8.804 ± 0.044

2.1 ± 0.021

4.817 ± 0.03

2.704 ± 0.033

10.638 ± 0.054

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.735 ± 0.022

2.258 ± 0.022

5.428 ± 0.027

3.281 ± 0.021

7.044 ± 0.04

4.866 ± 0.027

5.366 ± 0.03

7.436 ± 0.032

1.449 ± 0.018

2.025 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski