Charleville virus
Average proteome isoelectric point is 6.32
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A3Q8TND9|A0A3Q8TND9_9RHAB Gx OS=Charleville virus OX=318842 GN=Gx PE=4 SV=1
MM1 pKa = 7.51 NNLLSCKK8 pKa = 10.48 DD9 pKa = 4.17 KK10 pKa = 10.98 IPKK13 pKa = 9.71 NLLLAAIQVEE23 pKa = 4.71 SKK25 pKa = 10.95 NNEE28 pKa = 3.73 SSKK31 pKa = 10.98 LFGPQEE37 pKa = 4.04 FQEE40 pKa = 4.51 DD41 pKa = 4.63 SPTEE45 pKa = 3.96 SDD47 pKa = 3.55 GGSSSSHH54 pKa = 6.31 KK55 pKa = 10.66 SFTPASPVDD64 pKa = 3.58 ARR66 pKa = 11.84 CYY68 pKa = 8.21 TIPGIGLPKK77 pKa = 9.48 TQEE80 pKa = 3.86 EE81 pKa = 4.84 LNNRR85 pKa = 11.84 IASLLGAVFEE95 pKa = 4.6 TTGLSISGLKK105 pKa = 8.27 VTANSITFHH114 pKa = 6.59 EE115 pKa = 4.41 IEE117 pKa = 4.11 EE118 pKa = 4.66 DD119 pKa = 3.43 EE120 pKa = 4.71 LEE122 pKa = 4.09 EE123 pKa = 4.78 SEE125 pKa = 4.23 RR126 pKa = 11.84 TEE128 pKa = 4.02 SGGSSEE134 pKa = 5.64 LIKK137 pKa = 10.55 FDD139 pKa = 3.7 SKK141 pKa = 11.71 SPGGTNPFLPAEE153 pKa = 4.43 KK154 pKa = 9.86 SISPIPEE161 pKa = 3.97 VSSMIQDD168 pKa = 3.7 FLAEE172 pKa = 4.38 LEE174 pKa = 4.11 AGFYY178 pKa = 8.6 LTSITNKK185 pKa = 9.57 PLYY188 pKa = 10.24 VDD190 pKa = 3.91 RR191 pKa = 11.84 KK192 pKa = 10.29 KK193 pKa = 10.54 LTLTKK198 pKa = 10.55 EE199 pKa = 4.44 SILLWEE205 pKa = 4.5 TQPGWSKK212 pKa = 9.96 TKK214 pKa = 10.18 ILRR217 pKa = 11.84 EE218 pKa = 3.59 ACKK221 pKa = 9.73 RR222 pKa = 11.84 DD223 pKa = 3.09 KK224 pKa = 11.0 VLRR227 pKa = 11.84 GKK229 pKa = 10.68 LGMVSWTDD237 pKa = 3.36 SLQNDD242 pKa = 3.92 PP243 pKa = 5.11
Molecular weight: 26.66 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.056
IPC2_protein 5.054
IPC_protein 4.94
Toseland 4.825
ProMoST 5.029
Dawson 4.876
Bjellqvist 5.016
Wikipedia 4.711
Rodwell 4.8
Grimsley 4.736
Solomon 4.876
Lehninger 4.825
Nozaki 4.978
DTASelect 5.08
Thurlkill 4.8
EMBOSS 4.736
Sillero 5.054
Patrickios 4.393
IPC_peptide 4.889
IPC2_peptide 5.054
IPC2.peptide.svr19 5.044
Protein with the highest isoelectric point:
>tr|A0A3S8TMQ0|A0A3S8TMQ0_9RHAB Matrix protein OS=Charleville virus OX=318842 GN=M PE=4 SV=1
MM1 pKa = 7.6 LIEE4 pKa = 5.02 SGLKK8 pKa = 8.49 VTYY11 pKa = 10.02 QVGVQEE17 pKa = 4.31 EE18 pKa = 4.71 SVEE21 pKa = 4.1 KK22 pKa = 10.65 LLSEE26 pKa = 4.38 EE27 pKa = 4.22 VTGTRR32 pKa = 11.84 GRR34 pKa = 11.84 TLSKK38 pKa = 10.09 SYY40 pKa = 10.46 KK41 pKa = 9.72 RR42 pKa = 11.84 QGTLLSQFPNCC53 pKa = 4.95
Molecular weight: 5.92 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.112
IPC2_protein 7.059
IPC_protein 7.892
Toseland 7.717
ProMoST 8.053
Dawson 8.214
Bjellqvist 7.892
Wikipedia 8.17
Rodwell 8.141
Grimsley 7.249
Solomon 8.726
Lehninger 8.741
Nozaki 8.229
DTASelect 8.17
Thurlkill 8.214
EMBOSS 8.492
Sillero 8.492
Patrickios 4.596
IPC_peptide 8.726
IPC2_peptide 7.483
IPC2.peptide.svr19 7.722
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7
0
7
3518
53
2090
502.6
57.46
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
3.781 ± 0.825
2.16 ± 0.162
4.775 ± 0.396
7.106 ± 0.538
4.491 ± 0.31
6.168 ± 0.543
2.302 ± 0.291
8.13 ± 0.96
7.192 ± 0.3
9.75 ± 1.068
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.615 ± 0.304
5.088 ± 0.834
4.036 ± 0.619
3.07 ± 0.215
4.775 ± 0.443
7.789 ± 1.095
6.339 ± 0.166
5.003 ± 0.657
1.819 ± 0.288
3.61 ± 0.52
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here