Escherichia phage vB_EcoP_PhAPEC7

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Schitoviridae; Enquatrovirinae; Gamaleyavirus; Escherichia virus APEC7

Average proteome isoelectric point is 6.45

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 83 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A067ZGY3|A0A067ZGY3_9CAUD Uncharacterized protein OS=Escherichia phage vB_EcoP_PhAPEC7 OX=1391223 GN=PhAPEC7_59 PE=4 SV=1
MM1 pKa = 7.18ATVSKK6 pKa = 10.75EE7 pKa = 4.11SIEE10 pKa = 4.62DD11 pKa = 3.79KK12 pKa = 10.73IKK14 pKa = 10.71SVYY17 pKa = 9.65YY18 pKa = 10.26FNGADD23 pKa = 3.32AVKK26 pKa = 10.42SAFVDD31 pKa = 5.21PSALPADD38 pKa = 4.27DD39 pKa = 4.65LANLGLVTYY48 pKa = 8.96CIIILEE54 pKa = 4.26NGFKK58 pKa = 10.72VEE60 pKa = 4.31GVSACVDD67 pKa = 3.2PTIYY71 pKa = 10.74DD72 pKa = 3.4EE73 pKa = 4.35WKK75 pKa = 8.76GRR77 pKa = 11.84QYY79 pKa = 11.43AYY81 pKa = 10.51EE82 pKa = 3.98NAFDD86 pKa = 5.45KK87 pKa = 10.41IWEE90 pKa = 4.15MEE92 pKa = 4.4GYY94 pKa = 10.79LLRR97 pKa = 11.84QALHH101 pKa = 6.28EE102 pKa = 4.37KK103 pKa = 10.79EE104 pKa = 4.16EE105 pKa = 4.19TAKK108 pKa = 11.03ALASFAEE115 pKa = 4.27NNTCDD120 pKa = 4.75GGGCTII126 pKa = 4.85

Molecular weight: 13.88 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A067ZJU9|A0A067ZJU9_9CAUD Uncharacterized protein OS=Escherichia phage vB_EcoP_PhAPEC7 OX=1391223 GN=PhAPEC7_11 PE=4 SV=1
MM1 pKa = 7.67RR2 pKa = 11.84KK3 pKa = 9.51SLIMGTKK10 pKa = 9.64EE11 pKa = 3.63DD12 pKa = 3.52VSRR15 pKa = 11.84LRR17 pKa = 11.84NRR19 pKa = 11.84NVASKK24 pKa = 9.46RR25 pKa = 11.84TLSMTNEE32 pKa = 3.84QLQEE36 pKa = 4.45FIDD39 pKa = 4.23AGLKK43 pKa = 8.89QSKK46 pKa = 10.23ARR48 pKa = 11.84IVTYY52 pKa = 9.03NHH54 pKa = 6.82PCIKK58 pKa = 10.55

Molecular weight: 6.71 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

83

0

83

21963

40

3450

264.6

29.43

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.724 ± 0.569

0.92 ± 0.149

5.764 ± 0.145

6.475 ± 0.291

3.688 ± 0.178

6.816 ± 0.261

1.735 ± 0.145

5.787 ± 0.242

6.452 ± 0.291

8.136 ± 0.2

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.95 ± 0.168

5.714 ± 0.206

4.111 ± 0.174

4.207 ± 0.294

4.275 ± 0.149

6.083 ± 0.237

6.424 ± 0.257

6.816 ± 0.22

1.179 ± 0.114

3.743 ± 0.269
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski