Sporanaerobacter acetigenes DSM 13106
Average proteome isoelectric point is 6.16
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2836 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1M5WS51|A0A1M5WS51_9FIRM ATPase components of ABC transporters with duplicated ATPase domains OS=Sporanaerobacter acetigenes DSM 13106 OX=1123281 GN=SAMN02745180_01371 PE=4 SV=1
MM1 pKa = 7.54 TEE3 pKa = 4.38 DD4 pKa = 4.76 LNVSSPQKK12 pKa = 10.37 RR13 pKa = 11.84 GLFDD17 pKa = 3.26 ILGGGDD23 pKa = 3.72 SEE25 pKa = 4.37 NLLFFFLLLVVFFSNCGYY43 pKa = 10.98 GDD45 pKa = 4.03 RR46 pKa = 11.84 DD47 pKa = 3.85 DD48 pKa = 6.0 DD49 pKa = 3.98 SLLFFFLLLVVLFCYY64 pKa = 10.49 CC65 pKa = 4.21
Molecular weight: 7.4 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.754
IPC2_protein 3.808
IPC_protein 3.694
Toseland 3.49
ProMoST 3.923
Dawson 3.732
Bjellqvist 3.897
Wikipedia 3.732
Rodwell 3.541
Grimsley 3.414
Solomon 3.681
Lehninger 3.643
Nozaki 3.872
DTASelect 4.113
Thurlkill 3.592
EMBOSS 3.732
Sillero 3.834
Patrickios 0.604
IPC_peptide 3.681
IPC2_peptide 3.795
IPC2.peptide.svr19 3.758
Protein with the highest isoelectric point:
>tr|A0A1M5XZL6|A0A1M5XZL6_9FIRM Hook-length control protein FliK OS=Sporanaerobacter acetigenes DSM 13106 OX=1123281 GN=SAMN02745180_01926 PE=4 SV=1
MM1 pKa = 7.7 AKK3 pKa = 10.11 YY4 pKa = 10.61 CEE6 pKa = 3.97 ICGKK10 pKa = 10.53 GKK12 pKa = 10.84 VYY14 pKa = 10.69 GNQVTFSNKK23 pKa = 9.29 KK24 pKa = 9.84 NNRR27 pKa = 11.84 TWAPNVRR34 pKa = 11.84 RR35 pKa = 11.84 VKK37 pKa = 10.7 AIVDD41 pKa = 3.98 GSPKK45 pKa = 10.06 RR46 pKa = 11.84 IYY48 pKa = 10.82 VCTRR52 pKa = 11.84 CLRR55 pKa = 11.84 SGNVTRR61 pKa = 11.84 ALL63 pKa = 3.61
Molecular weight: 7.12 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.326
IPC2_protein 9.736
IPC_protein 10.145
Toseland 10.584
ProMoST 10.189
Dawson 10.701
Bjellqvist 10.365
Wikipedia 10.847
Rodwell 11.14
Grimsley 10.745
Solomon 10.76
Lehninger 10.73
Nozaki 10.599
DTASelect 10.35
Thurlkill 10.584
EMBOSS 10.965
Sillero 10.628
Patrickios 10.906
IPC_peptide 10.76
IPC2_peptide 9.516
IPC2.peptide.svr19 8.446
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2836
0
2836
879205
40
2831
310.0
35.13
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.227 ± 0.045
0.987 ± 0.017
5.855 ± 0.033
8.283 ± 0.055
4.316 ± 0.033
6.644 ± 0.044
1.328 ± 0.015
9.931 ± 0.052
8.947 ± 0.048
8.947 ± 0.038
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.731 ± 0.019
5.843 ± 0.039
3.004 ± 0.029
2.244 ± 0.02
3.625 ± 0.029
5.925 ± 0.033
4.707 ± 0.03
6.529 ± 0.037
0.693 ± 0.015
4.234 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here