Amino acid dipepetide frequency for Sporanaerobacter acetigenes DSM 13106

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	3.118AlaAla: 3.118 ± 0.084	0.589AlaCys: 0.589 ± 0.027	2.474AlaAsp: 2.474 ± 0.054	3.453AlaGlu: 3.453 ± 0.071	2.319AlaPhe: 2.319 ± 0.052	3.577AlaGly: 3.577 ± 0.082	0.816AlaHis: 0.816 ± 0.032	5.739AlaIle: 5.739 ± 0.094	4.435AlaLys: 4.435 ± 0.067	5.349AlaLeu: 5.349 ± 0.086	1.712AlaMet: 1.712 ± 0.045	2.63AlaAsn: 2.63 ± 0.057	1.384AlaPro: 1.384 ± 0.043	1.319AlaGln: 1.319 ± 0.043	2.014AlaArg: 2.014 ± 0.052	2.855AlaSer: 2.855 ± 0.061	2.58AlaThr: 2.58 ± 0.067	3.66AlaVal: 3.66 ± 0.076	0.313AlaTrp: 0.313 ± 0.02	1.938AlaTyr: 1.938 ± 0.047	0.0AlaXaa: 0.0 ± 0.0
Cys	0.441CysAla: 0.441 ± 0.025	0.114CysCys: 0.114 ± 0.013	0.605CysAsp: 0.605 ± 0.029	0.661CysGlu: 0.661 ± 0.025	0.382CysPhe: 0.382 ± 0.022	0.965CysGly: 0.965 ± 0.035	0.209CysHis: 0.209 ± 0.015	0.975CysIle: 0.975 ± 0.036	0.805CysLys: 0.805 ± 0.038	0.71CysLeu: 0.71 ± 0.03	0.249CysMet: 0.249 ± 0.016	0.532CysAsn: 0.532 ± 0.026	0.505CysPro: 0.505 ± 0.029	0.258CysGln: 0.258 ± 0.017	0.361CysArg: 0.361 ± 0.021	0.656CysSer: 0.656 ± 0.025	0.48CysThr: 0.48 ± 0.026	0.557CysVal: 0.557 ± 0.027	0.072CysTrp: 0.072 ± 0.009	0.33CysTyr: 0.33 ± 0.019	0.0CysXaa: 0.0 ± 0.0
Asp	2.4AspAla: 2.4 ± 0.055	0.523AspCys: 0.523 ± 0.024	3.016AspAsp: 3.016 ± 0.064	5.875AspGlu: 5.875 ± 0.094	2.898AspPhe: 2.898 ± 0.058	3.768AspGly: 3.768 ± 0.064	0.578AspHis: 0.578 ± 0.028	6.746AspIle: 6.746 ± 0.097	5.623AspLys: 5.623 ± 0.091	5.092AspLeu: 5.092 ± 0.085	1.858AspMet: 1.858 ± 0.044	3.014AspAsn: 3.014 ± 0.056	1.603AspPro: 1.603 ± 0.046	0.719AspGln: 0.719 ± 0.025	2.143AspArg: 2.143 ± 0.052	3.049AspSer: 3.049 ± 0.052	2.642AspThr: 2.642 ± 0.059	3.803AspVal: 3.803 ± 0.068	0.449AspTrp: 0.449 ± 0.028	2.751AspTyr: 2.751 ± 0.063	0.0AspXaa: 0.0 ± 0.0
Glu	4.126GluAla: 4.126 ± 0.067	0.578GluCys: 0.578 ± 0.029	5.212GluAsp: 5.212 ± 0.081	8.637GluGlu: 8.637 ± 0.122	2.957GluPhe: 2.957 ± 0.064	4.8GluGly: 4.8 ± 0.088	0.883GluHis: 0.883 ± 0.033	8.268GluIle: 8.268 ± 0.107	9.091GluLys: 9.091 ± 0.123	7.227GluLeu: 7.227 ± 0.092	2.39GluMet: 2.39 ± 0.056	5.878GluAsn: 5.878 ± 0.101	1.835GluPro: 1.835 ± 0.053	1.732GluGln: 1.732 ± 0.048	3.066GluArg: 3.066 ± 0.059	3.615GluSer: 3.615 ± 0.067	3.253GluThr: 3.253 ± 0.067	5.247GluVal: 5.247 ± 0.093	0.532GluTrp: 0.532 ± 0.029	3.503GluTyr: 3.503 ± 0.068	0.0GluXaa: 0.0 ± 0.0
Phe	2.216PheAla: 2.216 ± 0.056	0.438PheCys: 0.438 ± 0.024	2.629PheAsp: 2.629 ± 0.064	2.876PheGlu: 2.876 ± 0.066	1.987PhePhe: 1.987 ± 0.049	2.813PheGly: 2.813 ± 0.059	0.593PheHis: 0.593 ± 0.026	4.236PheIle: 4.236 ± 0.074	3.555PheLys: 3.555 ± 0.07	4.034PheLeu: 4.034 ± 0.081	1.154PheMet: 1.154 ± 0.035	2.484PheAsn: 2.484 ± 0.052	1.298PhePro: 1.298 ± 0.039	1.02PheGln: 1.02 ± 0.034	1.435PheArg: 1.435 ± 0.04	3.171PheSer: 3.171 ± 0.063	2.249PheThr: 2.249 ± 0.055	2.747PheVal: 2.747 ± 0.066	0.322PheTrp: 0.322 ± 0.019	1.906PheTyr: 1.906 ± 0.042	0.0PheXaa: 0.0 ± 0.0
Gly	3.765GlyAla: 3.765 ± 0.09	0.785GlyCys: 0.785 ± 0.029	3.294GlyAsp: 3.294 ± 0.066	5.193GlyGlu: 5.193 ± 0.087	3.096GlyPhe: 3.096 ± 0.067	4.54GlyGly: 4.54 ± 0.092	1.033GlyHis: 1.033 ± 0.04	6.837GlyIle: 6.837 ± 0.096	6.141GlyLys: 6.141 ± 0.091	5.724GlyLeu: 5.724 ± 0.091	1.969GlyMet: 1.969 ± 0.051	3.372GlyAsn: 3.372 ± 0.065	1.401GlyPro: 1.401 ± 0.043	1.587GlyGln: 1.587 ± 0.046	2.494GlyArg: 2.494 ± 0.063	3.598GlySer: 3.598 ± 0.061	3.449GlyThr: 3.449 ± 0.07	4.547GlyVal: 4.547 ± 0.084	0.485GlyTrp: 0.485 ± 0.025	3.127GlyTyr: 3.127 ± 0.059	0.0GlyXaa: 0.0 ± 0.0
His	0.641HisAla: 0.641 ± 0.029	0.185HisCys: 0.185 ± 0.014	0.663HisAsp: 0.663 ± 0.027	0.972HisGlu: 0.972 ± 0.032	0.593HisPhe: 0.593 ± 0.027	1.018HisGly: 1.018 ± 0.034	0.246HisHis: 0.246 ± 0.018	1.405HisIle: 1.405 ± 0.041	0.934HisLys: 0.934 ± 0.032	1.173HisLeu: 1.173 ± 0.039	0.357HisMet: 0.357 ± 0.023	0.665HisAsn: 0.665 ± 0.026	0.712HisPro: 0.712 ± 0.033	0.282HisGln: 0.282 ± 0.017	0.556HisArg: 0.556 ± 0.025	0.844HisSer: 0.844 ± 0.03	0.643HisThr: 0.643 ± 0.028	0.76HisVal: 0.76 ± 0.029	0.108HisTrp: 0.108 ± 0.011	0.519HisTyr: 0.519 ± 0.023	0.0HisXaa: 0.0 ± 0.0
Ile	5.748IleAla: 5.748 ± 0.1	1.053IleCys: 1.053 ± 0.034	6.661IleAsp: 6.661 ± 0.083	8.122IleGlu: 8.122 ± 0.115	4.223IlePhe: 4.223 ± 0.093	6.372IleGly: 6.372 ± 0.095	1.265IleHis: 1.265 ± 0.047	8.802IleIle: 8.802 ± 0.127	8.156IleLys: 8.156 ± 0.11	9.327IleLeu: 9.327 ± 0.132	2.437IleMet: 2.437 ± 0.063	5.479IleAsn: 5.479 ± 0.08	3.603IlePro: 3.603 ± 0.064	2.185IleGln: 2.185 ± 0.051	3.369IleArg: 3.369 ± 0.072	6.715IleSer: 6.715 ± 0.087	4.287IleThr: 4.287 ± 0.072	7.04IleVal: 7.04 ± 0.099	0.594IleTrp: 0.594 ± 0.026	3.872IleTyr: 3.872 ± 0.08	0.0IleXaa: 0.0 ± 0.0
Lys	4.544LysAla: 4.544 ± 0.076	0.717LysCys: 0.717 ± 0.035	6.187LysAsp: 6.187 ± 0.087	8.967LysGlu: 8.967 ± 0.118	2.977LysPhe: 2.977 ± 0.059	5.423LysGly: 5.423 ± 0.083	1.02LysHis: 1.02 ± 0.033	8.584LysIle: 8.584 ± 0.107	8.336LysLys: 8.336 ± 0.105	7.241LysLeu: 7.241 ± 0.087	2.465LysMet: 2.465 ± 0.053	6.063LysAsn: 6.063 ± 0.084	2.13LysPro: 2.13 ± 0.047	1.91LysGln: 1.91 ± 0.047	3.269LysArg: 3.269 ± 0.064	4.634LysSer: 4.634 ± 0.075	4.1LysThr: 4.1 ± 0.067	5.847LysVal: 5.847 ± 0.089	0.661LysTrp: 0.661 ± 0.028	4.396LysTyr: 4.396 ± 0.082	0.0LysXaa: 0.0 ± 0.0
Leu	4.904LeuAla: 4.904 ± 0.079	0.862LeuCys: 0.862 ± 0.031	5.644LeuAsp: 5.644 ± 0.081	7.174LeuGlu: 7.174 ± 0.097	3.742LeuPhe: 3.742 ± 0.079	6.178LeuGly: 6.178 ± 0.088	1.007LeuHis: 1.007 ± 0.038	7.912LeuIle: 7.912 ± 0.11	8.774LeuLys: 8.774 ± 0.107	7.654LeuLeu: 7.654 ± 0.112	2.268LeuMet: 2.268 ± 0.06	5.826LeuAsn: 5.826 ± 0.084	2.922LeuPro: 2.922 ± 0.058	1.841LeuGln: 1.841 ± 0.049	2.986LeuArg: 2.986 ± 0.057	6.31LeuSer: 6.31 ± 0.089	4.06LeuThr: 4.06 ± 0.073	5.505LeuVal: 5.505 ± 0.079	0.59LeuTrp: 0.59 ± 0.024	3.309LeuTyr: 3.309 ± 0.06	0.0LeuXaa: 0.0 ± 0.0
Met	1.909MetAla: 1.909 ± 0.049	0.214MetCys: 0.214 ± 0.016	1.811MetAsp: 1.811 ± 0.047	2.287MetGlu: 2.287 ± 0.057	1.021MetPhe: 1.021 ± 0.033	1.962MetGly: 1.962 ± 0.052	0.248MetHis: 0.248 ± 0.017	2.211MetIle: 2.211 ± 0.044	2.721MetLys: 2.721 ± 0.051	2.227MetLeu: 2.227 ± 0.048	0.698MetMet: 0.698 ± 0.03	1.769MetAsn: 1.769 ± 0.044	0.814MetPro: 0.814 ± 0.034	0.545MetGln: 0.545 ± 0.028	0.99MetArg: 0.99 ± 0.036	1.671MetSer: 1.671 ± 0.043	1.424MetThr: 1.424 ± 0.045	1.691MetVal: 1.691 ± 0.044	0.155MetTrp: 0.155 ± 0.013	0.942MetTyr: 0.942 ± 0.034	0.0MetXaa: 0.0 ± 0.0
Asn	2.515AsnAla: 2.515 ± 0.06	0.671AsnCys: 0.671 ± 0.028	2.64AsnAsp: 2.64 ± 0.057	4.445AsnGlu: 4.445 ± 0.087	2.508AsnPhe: 2.508 ± 0.061	3.604AsnGly: 3.604 ± 0.072	0.737AsnHis: 0.737 ± 0.033	6.932AsnIle: 6.932 ± 0.097	5.098AsnLys: 5.098 ± 0.088	5.383AsnLeu: 5.383 ± 0.08	1.803AsnMet: 1.803 ± 0.047	3.284AsnAsn: 3.284 ± 0.08	2.185AsnPro: 2.185 ± 0.056	1.35AsnGln: 1.35 ± 0.041	2.275AsnArg: 2.275 ± 0.052	3.603AsnSer: 3.603 ± 0.068	2.825AsnThr: 2.825 ± 0.061	3.611AsnVal: 3.611 ± 0.059	0.442AsnTrp: 0.442 ± 0.025	2.519AsnTyr: 2.519 ± 0.059	0.0AsnXaa: 0.0 ± 0.0
Pro	1.314ProAla: 1.314 ± 0.042	0.304ProCys: 0.304 ± 0.019	1.648ProAsp: 1.648 ± 0.047	2.431ProGlu: 2.431 ± 0.054	1.471ProPhe: 1.471 ± 0.039	1.882ProGly: 1.882 ± 0.055	0.572ProHis: 0.572 ± 0.026	3.039ProIle: 3.039 ± 0.068	2.42ProLys: 2.42 ± 0.05	2.591ProLeu: 2.591 ± 0.058	0.767ProMet: 0.767 ± 0.027	1.667ProAsn: 1.667 ± 0.039	0.742ProPro: 0.742 ± 0.032	0.856ProGln: 0.856 ± 0.031	1.008ProArg: 1.008 ± 0.036	1.78ProSer: 1.78 ± 0.046	1.662ProThr: 1.662 ± 0.045	2.176ProVal: 2.176 ± 0.052	0.241ProTrp: 0.241 ± 0.015	1.473ProTyr: 1.473 ± 0.044	0.0ProXaa: 0.0 ± 0.0
Gln	1.273GlnAla: 1.273 ± 0.044	0.216GlnCys: 0.216 ± 0.014	1.187GlnAsp: 1.187 ± 0.037	1.713GlnGlu: 1.713 ± 0.049	0.953GlnPhe: 0.953 ± 0.03	1.458GlnGly: 1.458 ± 0.04	0.26GlnHis: 0.26 ± 0.017	2.232GlnIle: 2.232 ± 0.052	2.062GlnLys: 2.062 ± 0.055	1.985GlnLeu: 1.985 ± 0.048	0.694GlnMet: 0.694 ± 0.027	1.416GlnAsn: 1.416 ± 0.045	0.533GlnPro: 0.533 ± 0.023	0.544GlnGln: 0.544 ± 0.029	0.975GlnArg: 0.975 ± 0.037	1.214GlnSer: 1.214 ± 0.045	0.934GlnThr: 0.934 ± 0.033	1.548GlnVal: 1.548 ± 0.045	0.199GlnTrp: 0.199 ± 0.013	1.042GlnTyr: 1.042 ± 0.033	0.0GlnXaa: 0.0 ± 0.0
Arg	2.01ArgAla: 2.01 ± 0.044	0.391ArgCys: 0.391 ± 0.021	2.202ArgAsp: 2.202 ± 0.049	3.438ArgGlu: 3.438 ± 0.076	1.479ArgPhe: 1.479 ± 0.042	2.277ArgGly: 2.277 ± 0.052	0.482ArgHis: 0.482 ± 0.026	3.389ArgIle: 3.389 ± 0.069	3.262ArgLys: 3.262 ± 0.063	3.114ArgLeu: 3.114 ± 0.066	0.992ArgMet: 0.992 ± 0.037	2.07ArgAsn: 2.07 ± 0.051	1.071ArgPro: 1.071 ± 0.039	1.076ArgGln: 1.076 ± 0.036	1.572ArgArg: 1.572 ± 0.045	1.564ArgSer: 1.564 ± 0.048	1.664ArgThr: 1.664 ± 0.044	2.342ArgVal: 2.342 ± 0.048	0.279ArgTrp: 0.279 ± 0.018	1.578ArgTyr: 1.578 ± 0.046	0.0ArgXaa: 0.0 ± 0.0
Ser	2.669SerAla: 2.669 ± 0.057	0.523SerCys: 0.523 ± 0.03	2.855SerAsp: 2.855 ± 0.058	4.039SerGlu: 4.039 ± 0.067	3.063SerPhe: 3.063 ± 0.074	4.354SerGly: 4.354 ± 0.078	0.979SerHis: 0.979 ± 0.033	6.606SerIle: 6.606 ± 0.095	5.026SerLys: 5.026 ± 0.074	5.626SerLeu: 5.626 ± 0.088	1.574SerMet: 1.574 ± 0.046	3.272SerAsn: 3.272 ± 0.07	1.823SerPro: 1.823 ± 0.051	1.623SerGln: 1.623 ± 0.043	2.135SerArg: 2.135 ± 0.046	3.89SerSer: 3.89 ± 0.071	3.008SerThr: 3.008 ± 0.055	3.278SerVal: 3.278 ± 0.058	0.408SerTrp: 0.408 ± 0.02	2.499SerTyr: 2.499 ± 0.057	0.0SerXaa: 0.0 ± 0.0
Thr	2.668ThrAla: 2.668 ± 0.056	0.431ThrCys: 0.431 ± 0.021	2.408ThrAsp: 2.408 ± 0.051	2.953ThrGlu: 2.953 ± 0.059	2.059ThrPhe: 2.059 ± 0.045	3.593ThrGly: 3.593 ± 0.069	0.786ThrHis: 0.786 ± 0.031	5.123ThrIle: 5.123 ± 0.074	3.608ThrLys: 3.608 ± 0.065	4.306ThrLeu: 4.306 ± 0.07	1.162ThrMet: 1.162 ± 0.038	2.568ThrAsn: 2.568 ± 0.05	1.735ThrPro: 1.735 ± 0.043	1.093ThrGln: 1.093 ± 0.035	1.706ThrArg: 1.706 ± 0.047	2.879ThrSer: 2.879 ± 0.057	2.568ThrThr: 2.568 ± 0.06	3.295ThrVal: 3.295 ± 0.068	0.332ThrTrp: 0.332 ± 0.019	1.794ThrTyr: 1.794 ± 0.042	0.0ThrXaa: 0.0 ± 0.0
Val	3.823ValAla: 3.823 ± 0.078	0.709ValCys: 0.709 ± 0.031	4.356ValAsp: 4.356 ± 0.073	5.672ValGlu: 5.672 ± 0.091	3.022ValPhe: 3.022 ± 0.058	4.5ValGly: 4.5 ± 0.087	0.862ValHis: 0.862 ± 0.034	5.351ValIle: 5.351 ± 0.082	5.331ValLys: 5.331 ± 0.076	6.333ValLeu: 6.333 ± 0.088	1.495ValMet: 1.495 ± 0.04	3.347ValAsn: 3.347 ± 0.071	2.201ValPro: 2.201 ± 0.051	1.466ValGln: 1.466 ± 0.04	2.036ValArg: 2.036 ± 0.049	4.183ValSer: 4.183 ± 0.071	2.752ValThr: 2.752 ± 0.055	4.928ValVal: 4.928 ± 0.087	0.357ValTrp: 0.357 ± 0.02	2.565ValTyr: 2.565 ± 0.057	0.0ValXaa: 0.0 ± 0.0
Trp	0.4TrpAla: 0.4 ± 0.022	0.09TrpCys: 0.09 ± 0.009	0.455TrpAsp: 0.455 ± 0.021	0.539TrpGlu: 0.539 ± 0.03	0.314TrpPhe: 0.314 ± 0.02	0.546TrpGly: 0.546 ± 0.027	0.099TrpHis: 0.099 ± 0.011	0.636TrpIle: 0.636 ± 0.028	0.533TrpLys: 0.533 ± 0.025	0.603TrpLeu: 0.603 ± 0.025	0.192TrpMet: 0.192 ± 0.016	0.449TrpAsn: 0.449 ± 0.023	0.156TrpPro: 0.156 ± 0.015	0.176TrpGln: 0.176 ± 0.013	0.257TrpArg: 0.257 ± 0.016	0.366TrpSer: 0.366 ± 0.022	0.321TrpThr: 0.321 ± 0.018	0.423TrpVal: 0.423 ± 0.023	0.086TrpTrp: 0.086 ± 0.01	0.292TrpTyr: 0.292 ± 0.022	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.789TyrAla: 1.789 ± 0.049	0.474TyrCys: 0.474 ± 0.024	2.606TyrAsp: 2.606 ± 0.063	3.383TyrGlu: 3.383 ± 0.069	2.1TyrPhe: 2.1 ± 0.059	2.806TyrGly: 2.806 ± 0.055	0.602TyrHis: 0.602 ± 0.026	4.288TyrIle: 4.288 ± 0.075	3.559TyrLys: 3.559 ± 0.064	3.769TyrLeu: 3.769 ± 0.072	1.071TyrMet: 1.071 ± 0.033	2.644TyrAsn: 2.644 ± 0.066	1.39TyrPro: 1.39 ± 0.042	0.854TyrGln: 0.854 ± 0.03	1.606TyrArg: 1.606 ± 0.044	2.655TyrSer: 2.655 ± 0.056	2.166TyrThr: 2.166 ± 0.053	2.283TyrVal: 2.283 ± 0.06	0.309TyrTrp: 0.309 ± 0.017	1.987TyrTyr: 1.987 ± 0.055	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 2836 proteins (879205 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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