Mycoplasma verecundum

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Tenericutes; Mollicutes; Mycoplasmatales; Mycoplasmataceae; Mycoplasmopsis

Average proteome isoelectric point is 7.02

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 671 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1T4LXJ3|A0A1T4LXJ3_9MOLU DNA-directed RNA polymerase subunit beta OS=Mycoplasma verecundum OX=171291 GN=rpoB PE=3 SV=1
MM1 pKa = 7.32FFSLSLNAISTIMNQFVNHH20 pKa = 7.31LISKK24 pKa = 10.08QITCKK29 pKa = 10.39SDD31 pKa = 2.71NCLYY35 pKa = 10.44CCNINHH41 pKa = 6.6IFKK44 pKa = 10.72SFSALCCKK52 pKa = 10.3QYY54 pKa = 11.33QSLFWKK60 pKa = 10.6LPIFNEE66 pKa = 4.49KK67 pKa = 9.52IDD69 pKa = 4.66FIDD72 pKa = 3.63PGIYY76 pKa = 10.02SDD78 pKa = 4.95DD79 pKa = 4.12DD80 pKa = 3.71KK81 pKa = 11.54LGPVYY86 pKa = 10.37MPDD89 pKa = 3.32DD90 pKa = 4.44VYY92 pKa = 11.59EE93 pKa = 4.18EE94 pKa = 4.58TPPTTTITFNEE105 pKa = 4.43FLNHH109 pKa = 6.51LLFFASNVFYY119 pKa = 11.04DD120 pKa = 4.09KK121 pKa = 11.1FLDD124 pKa = 3.92VYY126 pKa = 10.92NTFNDD131 pKa = 3.52SKK133 pKa = 11.48QDD135 pKa = 3.51FTLSSWNHH143 pKa = 5.56WYY145 pKa = 10.51FDD147 pKa = 5.05DD148 pKa = 7.12DD149 pKa = 5.87DD150 pKa = 5.92DD151 pKa = 6.05LLTKK155 pKa = 10.64LFVEE159 pKa = 4.49KK160 pKa = 10.49EE161 pKa = 4.47GNFSLEE167 pKa = 3.58MKK169 pKa = 8.9MFPKK173 pKa = 9.77IYY175 pKa = 9.5EE176 pKa = 4.2IPLLSYY182 pKa = 9.77WNQIMPKK189 pKa = 10.18EE190 pKa = 4.23KK191 pKa = 9.27ITNNEE196 pKa = 3.6YY197 pKa = 10.82LFNHH201 pKa = 7.6IIPKK205 pKa = 10.4AFFNSFPNNNISCYY219 pKa = 10.21DD220 pKa = 3.41FSEE223 pKa = 4.72PGVLPMEE230 pKa = 4.92DD231 pKa = 3.5EE232 pKa = 4.56YY233 pKa = 11.55EE234 pKa = 3.99

Molecular weight: 27.78 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1T4KUK5|A0A1T4KUK5_9MOLU 50S ribosomal protein L24 OS=Mycoplasma verecundum OX=171291 GN=rplX PE=3 SV=1
MM1 pKa = 7.34ARR3 pKa = 11.84KK4 pKa = 9.46ALIEE8 pKa = 3.93KK9 pKa = 10.2AKK11 pKa = 10.03RR12 pKa = 11.84HH13 pKa = 5.81PKK15 pKa = 10.03FSTRR19 pKa = 11.84AYY21 pKa = 8.48TRR23 pKa = 11.84CEE25 pKa = 3.78LCGRR29 pKa = 11.84PHH31 pKa = 6.83SVLRR35 pKa = 11.84KK36 pKa = 9.13YY37 pKa = 10.01KK38 pKa = 9.42VCRR41 pKa = 11.84ICFRR45 pKa = 11.84NLAHH49 pKa = 6.85EE50 pKa = 4.47GKK52 pKa = 10.15IPGMKK57 pKa = 9.27KK58 pKa = 10.56ASWW61 pKa = 3.19

Molecular weight: 7.16 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

671

0

671

266221

39

5067

396.8

45.37

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.758 ± 0.156

0.469 ± 0.034

6.034 ± 0.131

6.464 ± 0.105

4.746 ± 0.127

4.319 ± 0.113

1.38 ± 0.054

9.056 ± 0.158

9.633 ± 0.17

9.467 ± 0.077

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.811 ± 0.061

8.473 ± 0.2

2.748 ± 0.054

3.896 ± 0.105

2.771 ± 0.081

6.59 ± 0.074

5.464 ± 0.093

5.532 ± 0.097

0.896 ± 0.033

4.493 ± 0.098
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski