Streptococcus phage Javan215

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 5.73

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 55 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4D6A4C1|A0A4D6A4C1_9CAUD Uncharacterized protein OS=Streptococcus phage Javan215 OX=2548051 GN=Javan215_0036 PE=4 SV=1
MM1 pKa = 6.75QHH3 pKa = 6.51GLFGDD8 pKa = 4.1FDD10 pKa = 4.73YY11 pKa = 11.58DD12 pKa = 3.43NWLSTYY18 pKa = 9.72EE19 pKa = 3.84DD20 pKa = 3.81HH21 pKa = 7.87EE22 pKa = 4.74EE23 pKa = 4.13VFQGDD28 pKa = 3.2EE29 pKa = 4.05DD30 pKa = 3.8EE31 pKa = 6.53AYY33 pKa = 10.53DD34 pKa = 4.23RR35 pKa = 11.84WKK37 pKa = 10.85DD38 pKa = 3.88DD39 pKa = 5.17QLEE42 pKa = 4.14DD43 pKa = 3.92WNWW46 pKa = 2.82

Molecular weight: 5.79 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4D6A811|A0A4D6A811_9CAUD Uncharacterized protein OS=Streptococcus phage Javan215 OX=2548051 GN=Javan215_0009 PE=4 SV=1
MM1 pKa = 7.36KK2 pKa = 10.52VGFRR6 pKa = 11.84KK7 pKa = 9.78PSLKK11 pKa = 10.3KK12 pKa = 9.9SLKK15 pKa = 10.54ARR17 pKa = 11.84TTAKK21 pKa = 9.7WKK23 pKa = 10.05RR24 pKa = 11.84QAKK27 pKa = 9.32KK28 pKa = 10.61AIIPGYY34 pKa = 8.36GKK36 pKa = 10.54KK37 pKa = 10.71GIGWVKK43 pKa = 10.29NPKK46 pKa = 8.91KK47 pKa = 10.12AAYY50 pKa = 9.53NKK52 pKa = 9.48VYY54 pKa = 10.66HH55 pKa = 6.04KK56 pKa = 8.19TTFGLSDD63 pKa = 3.37VLKK66 pKa = 10.72LFKK69 pKa = 10.79

Molecular weight: 7.86 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

55

0

55

11937

45

1040

217.0

24.39

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.204 ± 0.429

0.746 ± 0.109

6.585 ± 0.318

7.556 ± 0.497

3.879 ± 0.262

7.003 ± 0.438

1.248 ± 0.143

5.965 ± 0.211

8.26 ± 0.438

7.858 ± 0.335

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.195 ± 0.144

6.09 ± 0.22

2.471 ± 0.143

3.77 ± 0.205

3.711 ± 0.318

6.325 ± 0.332

6.903 ± 0.428

6.618 ± 0.287

1.583 ± 0.169

4.029 ± 0.231
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski