Amino acid dipepetide frequency for Nitratireductor sp. SY7

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
18.453AlaAla: 18.453 ± 0.183
1.081AlaCys: 1.081 ± 0.031
7.389AlaAsp: 7.389 ± 0.083
8.762AlaGlu: 8.762 ± 0.109
4.928AlaPhe: 4.928 ± 0.057
11.999AlaGly: 11.999 ± 0.108
2.177AlaHis: 2.177 ± 0.041
7.087AlaIle: 7.087 ± 0.071
3.58AlaLys: 3.58 ± 0.056
13.163AlaLeu: 13.163 ± 0.115
3.669AlaMet: 3.669 ± 0.049
2.925AlaAsn: 2.925 ± 0.048
5.246AlaPro: 5.246 ± 0.078
3.262AlaGln: 3.262 ± 0.045
9.5AlaArg: 9.5 ± 0.095
6.497AlaSer: 6.497 ± 0.071
6.005AlaThr: 6.005 ± 0.064
9.386AlaVal: 9.386 ± 0.1
1.536AlaTrp: 1.536 ± 0.038
2.611AlaTyr: 2.611 ± 0.035
0.0AlaXaa: 0.0 ± 0.0
Cys
0.949CysAla: 0.949 ± 0.026
0.101CysCys: 0.101 ± 0.009
0.522CysAsp: 0.522 ± 0.017
0.454CysGlu: 0.454 ± 0.017
0.321CysPhe: 0.321 ± 0.013
0.935CysGly: 0.935 ± 0.028
0.266CysHis: 0.266 ± 0.015
0.359CysIle: 0.359 ± 0.016
0.154CysLys: 0.154 ± 0.01
0.734CysLeu: 0.734 ± 0.023
0.144CysMet: 0.144 ± 0.01
0.18CysAsn: 0.18 ± 0.012
0.425CysPro: 0.425 ± 0.018
0.204CysGln: 0.204 ± 0.011
0.657CysArg: 0.657 ± 0.022
0.426CysSer: 0.426 ± 0.018
0.388CysThr: 0.388 ± 0.019
0.609CysVal: 0.609 ± 0.021
0.114CysTrp: 0.114 ± 0.009
0.193CysTyr: 0.193 ± 0.012
0.0CysXaa: 0.0 ± 0.0
Asp
6.996AspAla: 6.996 ± 0.071
0.474AspCys: 0.474 ± 0.019
3.489AspAsp: 3.489 ± 0.061
3.856AspGlu: 3.856 ± 0.049
2.346AspPhe: 2.346 ± 0.043
5.759AspGly: 5.759 ± 0.075
1.279AspHis: 1.279 ± 0.032
3.345AspIle: 3.345 ± 0.047
1.711AspLys: 1.711 ± 0.035
5.606AspLeu: 5.606 ± 0.057
1.426AspMet: 1.426 ± 0.032
1.371AspAsn: 1.371 ± 0.032
3.433AspPro: 3.433 ± 0.058
1.517AspGln: 1.517 ± 0.033
4.684AspArg: 4.684 ± 0.062
2.0AspSer: 2.0 ± 0.039
2.556AspThr: 2.556 ± 0.056
4.153AspVal: 4.153 ± 0.053
0.948AspTrp: 0.948 ± 0.026
1.49AspTyr: 1.49 ± 0.037
0.0AspXaa: 0.0 ± 0.0
Glu
8.571GluAla: 8.571 ± 0.098
0.357GluCys: 0.357 ± 0.015
2.953GluAsp: 2.953 ± 0.05
3.874GluGlu: 3.874 ± 0.066
1.939GluPhe: 1.939 ± 0.041
4.653GluGly: 4.653 ± 0.058
1.243GluHis: 1.243 ± 0.03
3.792GluIle: 3.792 ± 0.056
2.569GluLys: 2.569 ± 0.053
5.754GluLeu: 5.754 ± 0.065
1.685GluMet: 1.685 ± 0.038
1.781GluAsn: 1.781 ± 0.033
3.088GluPro: 3.088 ± 0.055
1.971GluGln: 1.971 ± 0.034
5.598GluArg: 5.598 ± 0.073
2.38GluSer: 2.38 ± 0.045
3.826GluThr: 3.826 ± 0.05
4.029GluVal: 4.029 ± 0.055
0.787GluTrp: 0.787 ± 0.024
1.006GluTyr: 1.006 ± 0.028
0.0GluXaa: 0.0 ± 0.0
Phe
5.262PheAla: 5.262 ± 0.062
0.422PheCys: 0.422 ± 0.019
2.81PheAsp: 2.81 ± 0.05
2.385PheGlu: 2.385 ± 0.042
1.604PhePhe: 1.604 ± 0.045
3.929PheGly: 3.929 ± 0.067
0.795PheHis: 0.795 ± 0.023
1.707PheIle: 1.707 ± 0.04
0.892PheLys: 0.892 ± 0.024
3.61PheLeu: 3.61 ± 0.053
0.862PheMet: 0.862 ± 0.027
0.997PheAsn: 0.997 ± 0.033
1.666PhePro: 1.666 ± 0.035
0.948PheGln: 0.948 ± 0.028
2.521PheArg: 2.521 ± 0.045
2.298PheSer: 2.298 ± 0.042
1.852PheThr: 1.852 ± 0.032
3.174PheVal: 3.174 ± 0.054
0.591PheTrp: 0.591 ± 0.022
0.927PheTyr: 0.927 ± 0.027
0.0PheXaa: 0.0 ± 0.0
Gly
9.702GlyAla: 9.702 ± 0.097
0.832GlyCys: 0.832 ± 0.026
4.871GlyAsp: 4.871 ± 0.075
5.622GlyGlu: 5.622 ± 0.072
3.874GlyPhe: 3.874 ± 0.058
7.694GlyGly: 7.694 ± 0.109
2.033GlyHis: 2.033 ± 0.045
4.866GlyIle: 4.866 ± 0.069
3.18GlyLys: 3.18 ± 0.059
8.9GlyLeu: 8.9 ± 0.086
2.501GlyMet: 2.501 ± 0.04
2.231GlyAsn: 2.231 ± 0.044
3.535GlyPro: 3.535 ± 0.049
2.492GlyGln: 2.492 ± 0.042
6.649GlyArg: 6.649 ± 0.067
4.63GlySer: 4.63 ± 0.059
4.516GlyThr: 4.516 ± 0.061
6.188GlyVal: 6.188 ± 0.067
1.355GlyTrp: 1.355 ± 0.035
2.366GlyTyr: 2.366 ± 0.045
0.0GlyXaa: 0.0 ± 0.0
His
2.393HisAla: 2.393 ± 0.049
0.242HisCys: 0.242 ± 0.014
1.248HisAsp: 1.248 ± 0.03
1.119HisGlu: 1.119 ± 0.029
0.941HisPhe: 0.941 ± 0.029
1.997HisGly: 1.997 ± 0.04
0.55HisHis: 0.55 ± 0.021
0.865HisIle: 0.865 ± 0.025
0.465HisLys: 0.465 ± 0.017
2.032HisLeu: 2.032 ± 0.044
0.471HisMet: 0.471 ± 0.018
0.46HisAsn: 0.46 ± 0.033
1.286HisPro: 1.286 ± 0.029
0.543HisGln: 0.543 ± 0.019
1.462HisArg: 1.462 ± 0.033
0.919HisSer: 0.919 ± 0.026
0.765HisThr: 0.765 ± 0.021
1.613HisVal: 1.613 ± 0.031
0.272HisTrp: 0.272 ± 0.015
0.54HisTyr: 0.54 ± 0.02
0.0HisXaa: 0.0 ± 0.0
Ile
7.861IleAla: 7.861 ± 0.083
0.507IleCys: 0.507 ± 0.02
3.602IleAsp: 3.602 ± 0.051
3.708IleGlu: 3.708 ± 0.055
1.875IlePhe: 1.875 ± 0.05
5.083IleGly: 5.083 ± 0.054
0.923IleHis: 0.923 ± 0.026
2.251IleIle: 2.251 ± 0.046
1.269IleLys: 1.269 ± 0.03
4.429IleLeu: 4.429 ± 0.073
1.049IleMet: 1.049 ± 0.028
1.312IleAsn: 1.312 ± 0.034
2.208IlePro: 2.208 ± 0.04
1.053IleGln: 1.053 ± 0.031
3.374IleArg: 3.374 ± 0.047
2.819IleSer: 2.819 ± 0.049
2.506IleThr: 2.506 ± 0.038
4.722IleVal: 4.722 ± 0.062
0.572IleTrp: 0.572 ± 0.02
1.133IleTyr: 1.133 ± 0.033
0.0IleXaa: 0.0 ± 0.0
Lys
4.203LysAla: 4.203 ± 0.064
0.156LysCys: 0.156 ± 0.01
1.591LysAsp: 1.591 ± 0.036
1.625LysGlu: 1.625 ± 0.038
0.802LysPhe: 0.802 ± 0.024
2.544LysGly: 2.544 ± 0.045
0.588LysHis: 0.588 ± 0.02
1.548LysIle: 1.548 ± 0.033
1.309LysLys: 1.309 ± 0.035
3.054LysLeu: 3.054 ± 0.051
0.695LysMet: 0.695 ± 0.025
0.835LysAsn: 0.835 ± 0.028
1.966LysPro: 1.966 ± 0.044
0.868LysGln: 0.868 ± 0.026
2.316LysArg: 2.316 ± 0.038
1.732LysSer: 1.732 ± 0.035
1.816LysThr: 1.816 ± 0.047
2.438LysVal: 2.438 ± 0.041
0.341LysTrp: 0.341 ± 0.014
0.546LysTyr: 0.546 ± 0.022
0.0LysXaa: 0.0 ± 0.0
Leu
14.299LeuAla: 14.299 ± 0.112
0.838LeuCys: 0.838 ± 0.024
6.007LeuAsp: 6.007 ± 0.077
5.377LeuGlu: 5.377 ± 0.057
3.914LeuPhe: 3.914 ± 0.065
8.184LeuGly: 8.184 ± 0.076
1.676LeuHis: 1.676 ± 0.033
4.842LeuIle: 4.842 ± 0.073
3.477LeuLys: 3.477 ± 0.06
8.983LeuLeu: 8.983 ± 0.108
2.22LeuMet: 2.22 ± 0.043
2.262LeuAsn: 2.262 ± 0.037
5.255LeuPro: 5.255 ± 0.061
2.326LeuGln: 2.326 ± 0.044
6.517LeuArg: 6.517 ± 0.072
6.228LeuSer: 6.228 ± 0.07
5.052LeuThr: 5.052 ± 0.067
7.743LeuVal: 7.743 ± 0.085
1.118LeuTrp: 1.118 ± 0.027
1.928LeuTyr: 1.928 ± 0.039
0.0LeuXaa: 0.0 ± 0.0
Met
3.339MetAla: 3.339 ± 0.05
0.147MetCys: 0.147 ± 0.01
1.111MetAsp: 1.111 ± 0.03
1.177MetGlu: 1.177 ± 0.028
0.753MetPhe: 0.753 ± 0.024
1.801MetGly: 1.801 ± 0.035
0.473MetHis: 0.473 ± 0.018
1.423MetIle: 1.423 ± 0.034
0.997MetLys: 0.997 ± 0.025
2.52MetLeu: 2.52 ± 0.042
0.687MetMet: 0.687 ± 0.022
0.829MetAsn: 0.829 ± 0.022
1.483MetPro: 1.483 ± 0.034
0.778MetGln: 0.778 ± 0.023
1.938MetArg: 1.938 ± 0.032
1.675MetSer: 1.675 ± 0.034
1.769MetThr: 1.769 ± 0.038
1.764MetVal: 1.764 ± 0.039
0.228MetTrp: 0.228 ± 0.012
0.287MetTyr: 0.287 ± 0.016
0.0MetXaa: 0.0 ± 0.0
Asn
3.095AsnAla: 3.095 ± 0.043
0.238AsnCys: 0.238 ± 0.013
1.437AsnAsp: 1.437 ± 0.039
1.326AsnGlu: 1.326 ± 0.033
0.894AsnPhe: 0.894 ± 0.026
2.313AsnGly: 2.313 ± 0.043
0.508AsnHis: 0.508 ± 0.02
1.309AsnIle: 1.309 ± 0.029
0.614AsnLys: 0.614 ± 0.034
2.404AsnLeu: 2.404 ± 0.044
0.623AsnMet: 0.623 ± 0.022
0.614AsnAsn: 0.614 ± 0.026
1.786AsnPro: 1.786 ± 0.039
0.695AsnGln: 0.695 ± 0.023
1.857AsnArg: 1.857 ± 0.032
1.118AsnSer: 1.118 ± 0.03
1.143AsnThr: 1.143 ± 0.031
1.91AsnVal: 1.91 ± 0.041
0.388AsnTrp: 0.388 ± 0.018
0.635AsnTyr: 0.635 ± 0.02
0.0AsnXaa: 0.0 ± 0.0
Pro
6.313ProAla: 6.313 ± 0.084
0.283ProCys: 0.283 ± 0.014
3.704ProAsp: 3.704 ± 0.043
3.811ProGlu: 3.811 ± 0.061
2.074ProPhe: 2.074 ± 0.039
4.505ProGly: 4.505 ± 0.061
1.076ProHis: 1.076 ± 0.032
2.106ProIle: 2.106 ± 0.04
1.502ProLys: 1.502 ± 0.039
4.601ProLeu: 4.601 ± 0.056
1.09ProMet: 1.09 ± 0.027
1.193ProAsn: 1.193 ± 0.029
2.356ProPro: 2.356 ± 0.054
1.481ProGln: 1.481 ± 0.034
3.018ProArg: 3.018 ± 0.048
2.43ProSer: 2.43 ± 0.044
2.124ProThr: 2.124 ± 0.04
4.331ProVal: 4.331 ± 0.05
0.67ProTrp: 0.67 ± 0.02
1.16ProTyr: 1.16 ± 0.024
0.0ProXaa: 0.0 ± 0.0
Gln
3.589GlnAla: 3.589 ± 0.056
0.182GlnCys: 0.182 ± 0.011
1.268GlnAsp: 1.268 ± 0.028
1.473GlnGlu: 1.473 ± 0.035
0.992GlnPhe: 0.992 ± 0.026
2.029GlnGly: 2.029 ± 0.037
0.534GlnHis: 0.534 ± 0.019
1.585GlnIle: 1.585 ± 0.037
1.039GlnLys: 1.039 ± 0.031
2.528GlnLeu: 2.528 ± 0.047
0.791GlnMet: 0.791 ± 0.023
0.742GlnAsn: 0.742 ± 0.026
1.508GlnPro: 1.508 ± 0.034
0.962GlnGln: 0.962 ± 0.026
2.09GlnArg: 2.09 ± 0.044
1.51GlnSer: 1.51 ± 0.038
1.409GlnThr: 1.409 ± 0.03
1.983GlnVal: 1.983 ± 0.041
0.332GlnTrp: 0.332 ± 0.015
0.492GlnTyr: 0.492 ± 0.019
0.0GlnXaa: 0.0 ± 0.0
Arg
8.312ArgAla: 8.312 ± 0.083
0.485ArgCys: 0.485 ± 0.019
4.406ArgAsp: 4.406 ± 0.06
4.655ArgGlu: 4.655 ± 0.054
3.165ArgPhe: 3.165 ± 0.047
5.121ArgGly: 5.121 ± 0.064
1.886ArgHis: 1.886 ± 0.037
4.193ArgIle: 4.193 ± 0.053
2.433ArgLys: 2.433 ± 0.048
8.26ArgLeu: 8.26 ± 0.085
1.998ArgMet: 1.998 ± 0.037
1.877ArgAsn: 1.877 ± 0.035
3.668ArgPro: 3.668 ± 0.056
2.527ArgGln: 2.527 ± 0.046
6.237ArgArg: 6.237 ± 0.083
3.755ArgSer: 3.755 ± 0.058
3.37ArgThr: 3.37 ± 0.055
4.708ArgVal: 4.708 ± 0.052
1.001ArgTrp: 1.001 ± 0.026
1.704ArgTyr: 1.704 ± 0.034
0.0ArgXaa: 0.0 ± 0.0
Ser
6.141SerAla: 6.141 ± 0.065
0.404SerCys: 0.404 ± 0.016
2.946SerAsp: 2.946 ± 0.048
3.001SerGlu: 3.001 ± 0.051
2.314SerPhe: 2.314 ± 0.043
5.627SerGly: 5.627 ± 0.067
1.066SerHis: 1.066 ± 0.037
2.618SerIle: 2.618 ± 0.048
1.371SerLys: 1.371 ± 0.032
5.149SerLeu: 5.149 ± 0.056
1.284SerMet: 1.284 ± 0.025
1.168SerAsn: 1.168 ± 0.03
2.61SerPro: 2.61 ± 0.049
1.409SerGln: 1.409 ± 0.026
3.715SerArg: 3.715 ± 0.052
2.686SerSer: 2.686 ± 0.052
2.506SerThr: 2.506 ± 0.048
4.028SerVal: 4.028 ± 0.054
0.713SerTrp: 0.713 ± 0.022
1.232SerTyr: 1.232 ± 0.029
0.0SerXaa: 0.0 ± 0.0
Thr
6.126ThrAla: 6.126 ± 0.065
0.391ThrCys: 0.391 ± 0.016
2.691ThrAsp: 2.691 ± 0.041
2.698ThrGlu: 2.698 ± 0.047
1.979ThrPhe: 1.979 ± 0.039
5.194ThrGly: 5.194 ± 0.063
0.914ThrHis: 0.914 ± 0.024
2.906ThrIle: 2.906 ± 0.042
1.234ThrLys: 1.234 ± 0.032
5.322ThrLeu: 5.322 ± 0.072
1.25ThrMet: 1.25 ± 0.028
1.152ThrAsn: 1.152 ± 0.035
2.848ThrPro: 2.848 ± 0.046
1.1ThrGln: 1.1 ± 0.029
3.345ThrArg: 3.345 ± 0.05
2.559ThrSer: 2.559 ± 0.041
2.611ThrThr: 2.611 ± 0.049
4.295ThrVal: 4.295 ± 0.064
0.613ThrTrp: 0.613 ± 0.02
1.08ThrTyr: 1.08 ± 0.026
0.0ThrXaa: 0.0 ± 0.0
Val
10.014ValAla: 10.014 ± 0.089
0.601ValCys: 0.601 ± 0.019
4.366ValAsp: 4.366 ± 0.06
5.051ValGlu: 5.051 ± 0.066
3.047ValPhe: 3.047 ± 0.055
5.711ValGly: 5.711 ± 0.065
1.405ValHis: 1.405 ± 0.031
4.054ValIle: 4.054 ± 0.058
2.17ValLys: 2.17 ± 0.045
7.356ValLeu: 7.356 ± 0.088
1.931ValMet: 1.931 ± 0.041
1.971ValAsn: 1.971 ± 0.039
3.719ValPro: 3.719 ± 0.05
1.679ValGln: 1.679 ± 0.033
5.241ValArg: 5.241 ± 0.057
4.375ValSer: 4.375 ± 0.055
4.435ValThr: 4.435 ± 0.053
6.008ValVal: 6.008 ± 0.077
0.948ValTrp: 0.948 ± 0.027
1.498ValTyr: 1.498 ± 0.035
0.0ValXaa: 0.0 ± 0.0
Trp
1.228TrpAla: 1.228 ± 0.034
0.143TrpCys: 0.143 ± 0.009
0.608TrpAsp: 0.608 ± 0.023
0.583TrpGlu: 0.583 ± 0.023
0.548TrpPhe: 0.548 ± 0.021
0.866TrpGly: 0.866 ± 0.025
0.317TrpHis: 0.317 ± 0.016
0.631TrpIle: 0.631 ± 0.021
0.456TrpLys: 0.456 ± 0.019
1.604TrpLeu: 1.604 ± 0.037
0.364TrpMet: 0.364 ± 0.016
0.478TrpAsn: 0.478 ± 0.019
0.697TrpPro: 0.697 ± 0.02
0.576TrpGln: 0.576 ± 0.022
1.192TrpArg: 1.192 ± 0.027
0.811TrpSer: 0.811 ± 0.022
0.725TrpThr: 0.725 ± 0.024
0.768TrpVal: 0.768 ± 0.025
0.212TrpTrp: 0.212 ± 0.013
0.301TrpTyr: 0.301 ± 0.015
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.41TyrAla: 2.41 ± 0.036
0.25TyrCys: 0.25 ± 0.014
1.42TyrAsp: 1.42 ± 0.031
1.299TyrGlu: 1.299 ± 0.03
0.94TyrPhe: 0.94 ± 0.027
2.092TyrGly: 2.092 ± 0.035
0.474TyrHis: 0.474 ± 0.019
0.771TyrIle: 0.771 ± 0.023
0.552TyrLys: 0.552 ± 0.022
2.238TyrLeu: 2.238 ± 0.04
0.439TyrMet: 0.439 ± 0.018
0.529TyrAsn: 0.529 ± 0.022
1.044TyrPro: 1.044 ± 0.026
0.648TyrGln: 0.648 ± 0.023
1.848TyrArg: 1.848 ± 0.04
1.141TyrSer: 1.141 ± 0.03
0.934TyrThr: 0.934 ± 0.025
1.731TyrVal: 1.731 ± 0.037
0.367TyrTrp: 0.367 ± 0.015
0.55TyrTyr: 0.55 ± 0.02
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 4636 proteins (1446749 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski