Halalkalicoccus jeotgali (strain DSM 18796 / CECT 7217 / JCM 14584 / KCTC 4019 / B3)
Average proteome isoelectric point is 5.02
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3779 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|D8JB89|D8JB89_HALJB ATP/cobalamin adenosyltransferase OS=Halalkalicoccus jeotgali (strain DSM 18796 / CECT 7217 / JCM 14584 / KCTC 4019 / B3) OX=795797 GN=HacjB3_15921 PE=4 SV=1
MM1 pKa = 7.88 SGEE4 pKa = 3.81 EE5 pKa = 3.81 ALYY8 pKa = 10.75 VVDD11 pKa = 5.94 DD12 pKa = 4.07 SGHH15 pKa = 4.79 VLRR18 pKa = 11.84 GVNNGGDD25 pKa = 3.95 VTWDD29 pKa = 3.48 EE30 pKa = 4.42 PQTPGDD36 pKa = 3.84 GSDD39 pKa = 2.94 ITSVVFTDD47 pKa = 3.78 EE48 pKa = 5.61 AGFLCDD54 pKa = 3.54 TNAGVYY60 pKa = 7.56 EE61 pKa = 4.26 TTDD64 pKa = 5.58 DD65 pKa = 3.63 GDD67 pKa = 3.23 TWDD70 pKa = 4.54 RR71 pKa = 11.84 IGIEE75 pKa = 3.81 EE76 pKa = 4.91 AGPDD80 pKa = 3.66 FTDD83 pKa = 3.37 IAPARR88 pKa = 11.84 RR89 pKa = 11.84 DD90 pKa = 4.02 AISVSCDD97 pKa = 2.99 DD98 pKa = 3.86 GTVFRR103 pKa = 11.84 YY104 pKa = 10.46 DD105 pKa = 3.06 GTNWTNRR112 pKa = 11.84 YY113 pKa = 9.06 IGEE116 pKa = 4.14 EE117 pKa = 3.99 ALCAIDD123 pKa = 5.31 RR124 pKa = 11.84 NTDD127 pKa = 2.98 TGLLCGEE134 pKa = 5.16 DD135 pKa = 3.42 GAIYY139 pKa = 9.15 EE140 pKa = 4.25 QQQGRR145 pKa = 11.84 WEE147 pKa = 4.1 RR148 pKa = 11.84 QPTPIEE154 pKa = 4.11 EE155 pKa = 4.32 TLHH158 pKa = 6.39 GIAIGTEE165 pKa = 4.19 YY166 pKa = 10.43 PSVAVGEE173 pKa = 4.65 DD174 pKa = 3.41 GTIIEE179 pKa = 4.65 YY180 pKa = 10.36 VV181 pKa = 3.13
Molecular weight: 19.6 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.708
IPC2_protein 3.668
IPC_protein 3.668
Toseland 3.452
ProMoST 3.834
Dawson 3.668
Bjellqvist 3.821
Wikipedia 3.592
Rodwell 3.503
Grimsley 3.363
Solomon 3.656
Lehninger 3.605
Nozaki 3.783
DTASelect 3.999
Thurlkill 3.503
EMBOSS 3.605
Sillero 3.795
Patrickios 1.062
IPC_peptide 3.643
IPC2_peptide 3.77
IPC2.peptide.svr19 3.733
Protein with the highest isoelectric point:
>tr|D8J297|D8J297_HALJB Sodium/hydrogen exchanger OS=Halalkalicoccus jeotgali (strain DSM 18796 / CECT 7217 / JCM 14584 / KCTC 4019 / B3) OX=795797 GN=HacjB3_07345 PE=4 SV=1
MM1 pKa = 7.81 ILDD4 pKa = 4.01 SLLQNFMMRR13 pKa = 11.84 SCLMRR18 pKa = 11.84 NSAAPISIEE27 pKa = 3.99 RR28 pKa = 11.84 SSKK31 pKa = 10.95 ALISLVRR38 pKa = 11.84 TLLSTLLVGLTILAQSSMILTYY60 pKa = 9.55 FMRR63 pKa = 11.84 IFVVLNSLTWCFATRR78 pKa = 11.84 ISLEE82 pKa = 4.22 VIILTLTLEE91 pKa = 3.98 MLILVMQHH99 pKa = 6.08 FIMRR103 pKa = 11.84 FSHH106 pKa = 6.33 ALIVAEE112 pKa = 4.2 RR113 pKa = 11.84 HH114 pKa = 5.71 LPMLYY119 pKa = 10.38 FMRR122 pKa = 11.84 RR123 pKa = 11.84 FLQMLGLTRR132 pKa = 11.84 KK133 pKa = 8.31 RR134 pKa = 11.84 HH135 pKa = 4.71 SMIQAFLDD143 pKa = 3.1 QWLSMKK149 pKa = 8.66 KK150 pKa = 7.09 THH152 pKa = 6.27 MLWKK156 pKa = 9.46 DD157 pKa = 3.76 CPWILIHH164 pKa = 7.31
Molecular weight: 19.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.349
IPC2_protein 9.633
IPC_protein 10.526
Toseland 10.789
ProMoST 10.54
Dawson 10.862
Bjellqvist 10.599
Wikipedia 11.096
Rodwell 11.023
Grimsley 10.906
Solomon 11.023
Lehninger 10.994
Nozaki 10.789
DTASelect 10.599
Thurlkill 10.789
EMBOSS 11.199
Sillero 10.804
Patrickios 10.789
IPC_peptide 11.038
IPC2_peptide 9.867
IPC2.peptide.svr19 8.598
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3779
0
3779
1011784
30
1575
267.7
29.2
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
10.477 ± 0.046
0.772 ± 0.013
7.393 ± 0.05
9.176 ± 0.064
3.42 ± 0.028
8.784 ± 0.048
2.032 ± 0.024
4.68 ± 0.033
1.882 ± 0.024
9.177 ± 0.051
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.898 ± 0.017
2.361 ± 0.024
4.691 ± 0.027
2.362 ± 0.025
6.688 ± 0.048
5.517 ± 0.029
6.231 ± 0.031
8.497 ± 0.045
1.152 ± 0.015
2.81 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here