Halalkalicoccus jeotgali (strain DSM 18796 / CECT 7217 / JCM 14584 / KCTC 4019 / B3)

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Stenosarchaea group; Halobacteria; Halobacteriales; Halobacteriaceae; Halalkalicoccus; Halalkalicoccus jeotgali

Average proteome isoelectric point is 5.02

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3779 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|D8JB89|D8JB89_HALJB ATP/cobalamin adenosyltransferase OS=Halalkalicoccus jeotgali (strain DSM 18796 / CECT 7217 / JCM 14584 / KCTC 4019 / B3) OX=795797 GN=HacjB3_15921 PE=4 SV=1
MM1 pKa = 7.88SGEE4 pKa = 3.81EE5 pKa = 3.81ALYY8 pKa = 10.75VVDD11 pKa = 5.94DD12 pKa = 4.07SGHH15 pKa = 4.79VLRR18 pKa = 11.84GVNNGGDD25 pKa = 3.95VTWDD29 pKa = 3.48EE30 pKa = 4.42PQTPGDD36 pKa = 3.84GSDD39 pKa = 2.94ITSVVFTDD47 pKa = 3.78EE48 pKa = 5.61AGFLCDD54 pKa = 3.54TNAGVYY60 pKa = 7.56EE61 pKa = 4.26TTDD64 pKa = 5.58DD65 pKa = 3.63GDD67 pKa = 3.23TWDD70 pKa = 4.54RR71 pKa = 11.84IGIEE75 pKa = 3.81EE76 pKa = 4.91AGPDD80 pKa = 3.66FTDD83 pKa = 3.37IAPARR88 pKa = 11.84RR89 pKa = 11.84DD90 pKa = 4.02AISVSCDD97 pKa = 2.99DD98 pKa = 3.86GTVFRR103 pKa = 11.84YY104 pKa = 10.46DD105 pKa = 3.06GTNWTNRR112 pKa = 11.84YY113 pKa = 9.06IGEE116 pKa = 4.14EE117 pKa = 3.99ALCAIDD123 pKa = 5.31RR124 pKa = 11.84NTDD127 pKa = 2.98TGLLCGEE134 pKa = 5.16DD135 pKa = 3.42GAIYY139 pKa = 9.15EE140 pKa = 4.25QQQGRR145 pKa = 11.84WEE147 pKa = 4.1RR148 pKa = 11.84QPTPIEE154 pKa = 4.11EE155 pKa = 4.32TLHH158 pKa = 6.39GIAIGTEE165 pKa = 4.19YY166 pKa = 10.43PSVAVGEE173 pKa = 4.65DD174 pKa = 3.41GTIIEE179 pKa = 4.65YY180 pKa = 10.36VV181 pKa = 3.13

Molecular weight:
19.6 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|D8J297|D8J297_HALJB Sodium/hydrogen exchanger OS=Halalkalicoccus jeotgali (strain DSM 18796 / CECT 7217 / JCM 14584 / KCTC 4019 / B3) OX=795797 GN=HacjB3_07345 PE=4 SV=1
MM1 pKa = 7.81ILDD4 pKa = 4.01SLLQNFMMRR13 pKa = 11.84SCLMRR18 pKa = 11.84NSAAPISIEE27 pKa = 3.99RR28 pKa = 11.84SSKK31 pKa = 10.95ALISLVRR38 pKa = 11.84TLLSTLLVGLTILAQSSMILTYY60 pKa = 9.55FMRR63 pKa = 11.84IFVVLNSLTWCFATRR78 pKa = 11.84ISLEE82 pKa = 4.22VIILTLTLEE91 pKa = 3.98MLILVMQHH99 pKa = 6.08FIMRR103 pKa = 11.84FSHH106 pKa = 6.33ALIVAEE112 pKa = 4.2RR113 pKa = 11.84HH114 pKa = 5.71LPMLYY119 pKa = 10.38FMRR122 pKa = 11.84RR123 pKa = 11.84FLQMLGLTRR132 pKa = 11.84KK133 pKa = 8.31RR134 pKa = 11.84HH135 pKa = 4.71SMIQAFLDD143 pKa = 3.1QWLSMKK149 pKa = 8.66KK150 pKa = 7.09THH152 pKa = 6.27MLWKK156 pKa = 9.46DD157 pKa = 3.76CPWILIHH164 pKa = 7.31

Molecular weight:
19.31 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3779

0

3779

1011784

30

1575

267.7

29.2

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.477 ± 0.046

0.772 ± 0.013

7.393 ± 0.05

9.176 ± 0.064

3.42 ± 0.028

8.784 ± 0.048

2.032 ± 0.024

4.68 ± 0.033

1.882 ± 0.024

9.177 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.898 ± 0.017

2.361 ± 0.024

4.691 ± 0.027

2.362 ± 0.025

6.688 ± 0.048

5.517 ± 0.029

6.231 ± 0.031

8.497 ± 0.045

1.152 ± 0.015

2.81 ± 0.024

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski